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WorksheetsFinal Exam Review- MDL 328A
Total questions: 35
Worksheet time: 18mins
What is the principle of the reagent strip test for urine protein?
Copper reduction
An enzymatic reaction
The toluidine reaction
Protein error of indicators
The intensity of colour produced in the Biuret reaction depends most on which of the following?
Molecular weight of the protein
Which protein are present
The number of peptide bonds
The A/G ratio
What is the result to the rate of an enzymatic reaction with increase in temperature from 25oC to 35oC?
Half
Double
Remains the same
Decreases
In monoclonal gammopathy, a large increase in which protein fraction is excepted on serum protein electrophoresis?
Albumin
Alpha 1
Alpha 2
Gamma
To what major class of enzyme does amylase belong?
Hydrolase
Oxidoreductase
Transferase
Isomerase
Which lipoprotein carries most of the endogenous triglycerides?
LDL
VLDL
HDL
Chylomicrons
LD catalyzes the conversion of lactic acid to which substance?
Pyruvate
Citric acid
Oxaloacetic acid
Malic acid
Which isoenzyme of CK has the slowest electrophoretic mobility at pH 8.6?
MM
MB
BB
Macro CK
How many subunits are LD isoenzymes composed of?
1
2
3
4
Which of the following apolipoproteins is essential for chylomicron formation?
B-100
B-48
CII
AI
Which protein listed migrates in the beta region at pH 8.6?
Haptoglobin
Anti-trypsin
IgG
Transferrin
The enzyme that is increased significantly during pernicious anemia is:
LD
CK
AST
ALP
Which 2 molecules combine to form ketone bodies?
Acetyl CoA and a ketoacid
Acetyl CoA and a fatty acid
Acetyl CoA and glycerol
2 Acetyl CoA
Which urine protein result would correlate best with a patient with nephrotic syndrome?
The lipoprotein that contains the greatest amount of protein is called:
The protein component of a lipoprotein is referred to as:
Terpene
Apolipoprotein
Prostaglandin
Phospholipid
Which protein is found in serum, CSF, interstitial fluid, and amniotic fluid?
Transferrin
Marcoglobulin
Albumin
Beta-globulin
Which enzyme can be estimated in both serum and urine?
ALP
LD
CK
AMY
A type of cancer diagnosed in part by ID of a paraprotein in blood or urine is:
Multiple myeloma
Bence-Jones proteins
Hypogammaglobulinemia
Wilson disease
Which term describes a condition that shows a split in the albumin band on protein electrophoresis?
Analbuminemia
Anodic albuminemia
Prealbuminemia
Bisalbuminemia
At pH 8.6, what causes the cathodal movement of gamma-globulins?
Endosmosis
Wick flow
A net positive charge
Cathodal sample application
Which is the most sensitive marker for alcoholic liver disease?
GLD
ALT
AST
GGT
Which protein is most responsible for maintaining osmotic pressure?
Which protein has the highest dye-binding capacity?
Albumin
Alpha globulin
Beta globulin
Gamma globulin
What is the reference range for serum albumin?
3.5-5.5 g/L
35-55 g/L
38-50 mmol/L
35-55 mg/L
The routine Biuret method is NOT used for urine or CSF protein determinations due to which of the following reasons?
Absorbance shifts
Interferences present in sample
Precipitation of protein
Poor sensitivity
Exudate fluid would most likely be formed as a result of which of the following?
Infection
Blocked lymphatic drainage
Disturbance of cardiac function
Increased hydrostatic pressure
Under specified conditions of pH and temperature control, which of the following statements describes the Katal unit?
The time require to utilize one mole of substrate
Utilization of 1 mole of substrate per second
Milligrams of product formed per hour
Utilization of 1 micromole of substrate per minute
When analytical procedures use enzymes as reagents, which statement is correct?
The timing of the final reading is usually not critical
Excess substrate is required to ensure zero order kinetics
Readings must be taken at frequent intervals
Readings must be taken during maximum and constant rate period
What best describes triglycerides?
Anhydrides of glycerol and fatty acids
Chain of amino acids
Utilized for steroid production
Hydrophilic
Which enzyme is most specific for diagnosing acute pancreatitis?
AMY
AST
LD
CK
Which protein fraction is most likely to increase in nephrotic syndrome?
Gamma globulin
Beta globulin
Alpha 2 globulin
Albumin
Which enzyme is commonly used as a marker for myocardial infarction?
LDH
ALP
CK-MB
AMY
Which lipoprotein is primarily responsible for transporting cholesterol from tissues to the liver?
VLDL
HDL
LDL
Chylomicrons
Which lipoprotein is primarily responsible for transporting dietary triglycerides?
Chylomicrons
VLDL
LDL
HDL
