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Biotechnology

Total questions: 70

Worksheet time: 1hrs 16mins

Name
Class
Date
1.
The process of changing the genetic material of a living organism 
a)
Genetic Modification
b)
Taxonomy
c)
Adapting
d)
Bioremediation
2.

This is the practice of producing a genetically identical cell or organism

a)

Cloning

b)

Gel Electrophoresis

c)

Genetic Engineering

d)

DNA Fingerprinting

3.
A lab technique used in crime fighting to prove a suspects innocence or guilt is known as:
a)
genetic engineering
b)
PCR
c)
electrophoresis
d)
gene therapy
4.
What is recombinant DNA?
a)
Adding DNA from one organism into the DNA of another
b)
DNA which has been changed over generations by natural selection
c)
DNA that causes genetic disorders
d)
DNA that has been sequenced
5.

Why is Dolly the sheep considered a clone?

a)

the source of Dolly's DNA is a single cell taken from an adult donor

b)

the DNA molecules in each of Dolly's cells are different

c)

Dolly has a mix of genes from her foster mother and the sheep that donated a nucleus of one of its cells

d)

Dolly is genetically identical to her offspring

6.
Another name for GMO (Genetically Modified Organsim) is ____________.
a)
Transgenic
b)
Transplanted
c)
Transducted
d)
Plasmid
7.
True or False: Gel electrophoresis allow scientist to compare DNA of different individuals.
a)
True
b)
False
8.

The laboratory process that makes many copies of DNA

a)

PCR

b)

Electrophoresis

c)

Gene Therapy

9.

The process that has been used for centruries to create purebred animals such as black labs is called

a)

recombinant DNA

b)

cloning

c)

selective breeding

10.

T or F Clones like Dolly are created asexually from a donor cells DNA.

a)

True

b)

False

11.

Problems with cloning are

a)

creating too many embryos that are unneeded

b)

premature aging and a high failure rate

c)

they have a tendency to overpopulate

12.

The insertion of a gene of interest into a bacterial DNA loop is called

a)

recombinant DNA

b)

insertion technique

c)

mutation propagation

13.

Which of the following is an example of a GMO

a)

Bt Corn

b)

Black Lab Puppies

c)

Dolly the sheep

14.

The graphic shown here is called a

a)

PCR

b)

DNA fingerprint

c)

electrophoresis gel

15.

A company that creates hair dye would most likely employ a bio-technician for which job?

a)

researching trends

b)

developing new colors

c)

creating cost effective packaging

d)

developing a product that will be safe for consumers

16.

Which are most closely related to biotechnology?

a)

medicine and agriculture

b)

construction and engineering

c)

water treatment and electricity generation

d)

communication and information technology

17.

Which is a concern of scientists when genetically modifying plants?

a)

Plants will pass on diseases to animals.

b)

There will be a decrease in biodiversity.

c)

Plants will have a longer growing season.

d)

There will be a decrease in revenue for pesticide manufacturers.

18.

How does biotechnology most likely affect North Carolina’s population?

a)

Jobs are created and attract more people to the area.

b)

Research advancement causes people in need of medicines to move to the area.

c)

People are not concerned with biotechnology so the overall population is not impacted.

d)

The issue causes controversy and people hesitate to live in a state where a large amount of research takes place.

19.

Which best describes a controversial issue associated with the use of genetically modified crops?

a)

the use of genetically modified crops to increase potential yield

b)

the short term use of genetically modified crops in famine stricken countries

c)

the development of genetically modified crops which are resistant to herbicides

d)

the long term effects which may arise from the use of genetically modified crops

20.

Which area of biotechnology would most likely create ethical issues within human society?

a)

insulin production by bacteria

b)

organ cloning for use in transplants

c)

genetic engineering to improve agricultural yields

d)

DNA and forensic testing of crime scene evidence

21.

Which was most likely the earliest way biotechnology was studied by humans?

a)

finding evidence of crimes

b)

making new medicines to cure sicknesses

c)

discovering the cause of hereditary diseases

d)

breeding better varieties of crops and livestock

22.

Which is a genetic engineering advancement directly related to a career in biotechnology?

a)

improving solar energy collection

b)

transporting textiles at higher rates

c)

creating crop foods that resist insect pests

d)

repairing historical sites with quality materials

23.
Which field would most likely NOT incorporate  biotechnology?
a)
Agriculture 
b)
Genetics 
c)
Medicine
d)
Mining 
24.
_________________ is NOT a common ethical  concern related to human cloning.
a)
That humans will be created who exist solely as a supply of organs for transplants
b)
That clones will be more prone to abnormal development
c)
That parents will pay to have a child that is a clone of a famous athlete
d)
That cloned humans will have no personality
25.
Which of these is not a way in which biotechnology can benefit agriculture?
a)
increase crop population
b)
reducing the loss of crops to insects
c)
producing better-tasting fruits
d)
improving farm machinery
26.
A genome is
a)
part of a chromosome
b)
a gene inserted into a chromosome of bacteria
c)
a sequence of units in a short segment of DNA
d)
the complete sequence of an organism’s DNA  
27.
___________ changes the genetic material of a living organism.
a)
Crossbreeding 
b)
Genetic Modification 
c)
Bioremediation
d)
Genome
28.
______________uses bacteria to clean up our surrounding.
a)
Genome
b)
Bioremediation 
c)
Genetic Modification 
d)
Crossbreeding 
29.
Which of the following would be an example of gene therapy technology?
a)
development of a nasal spray that contains copies of the normal gene that is defective in person with cystic fibrosis
b)
cutting DNA into fragments with restriction enzymes
c)
modifying E. coli to produce indigo dye for coloring denim blue jeans
d)
separation DNA fragments using gel electrophoresis
30.
The Human Genome Project has made diagnosing genetic disorders easier.Once a genetic disorder is diagnosed, ____ can be used as a possible treatment.
a)
cell cultures
b)
gene therapy
c)
DNA fingerprinting
d)
PCR
31.
The entire collection of genes within human cells is referred to as the ______.
a)
human genome
b)
karyotype
c)
pedigree
d)
gene map
32.
This science allows us to alter the DNA of an orange tree so the fruit contains more vitamin C.
a)
Physical science
b)
Biotechnology
c)
Biology
d)
Geology
33.
Which of the below is an ethical issue in the field biotechnology?
a)
Outrageous cost - very expensive
b)
We can live longer and healthier lives
c)
Sometimes the harm outweighs the good
d)
Public opinion of the technology
34.
Which of the below biotechnological advances is MOST beneficial to Florida FARMERS?
a)
Development of disease resistant plants for higher yields
b)
Development of more effective human vaccines
c)
The ability to create more antibiotics from bacteria
d)
Producing enzymes to eliminate pollutants
35.

GEAC stands for

a)

Genome Engineering Action Committee

b)

Ground Environment Action Committee

c)

Genetic Engineering Approval Committee

d)

Genetic and Environment Approval Committee.

36.

α – 1 – antitrypsin is

a)

an antacid

b)

an enzyme

c)

used to treat arthritis

d)

used to treat emphysema.

37.

The site of production of ADA in the body is

a)

erythrocytes

b)

lymphocytes

c)

blood plasma

d)

osteocytes.

38.

Golden rice is

a)

a variety of rice grown along the yellow river in China

b)

long stored rice having yellow colour tint

c)

a transgenic rice having gene for β -carotene

d)

wild variety of rice with yellow coloured grains.

39.

In RNAi, genes are silenced using

a)

ssDNA

b)

dsDNA

c)

dsRNA

d)

ssRNA.

40.

Which one of the following is not used as biofertiliser ?

a)

Bacillus thuringiensis

b)

Anabaena

c)

Nostoc

d)

Rhizobium

41.

Which variety of rice was patented by a U.S. company even though the highest number of varieties of this rice are found in India ?

a)

Sharbati Sonora

b)

Co-667

c)

Basmati

d)

LermaRojo

42.

C-peptide of human insulin is

a)

a part of mature insulin molecule

b)

responsible for formation of disulphide bridges

c)

removed during maturation of pro-insulin to insulin

d)

responsible for its biological activity.

Answer:

43.

Which of the following genes were introduced in cotton to protect it from cotton bollworms ?

a)

Cry Ac and CryAb

b)

BtAc and Btab

c)

CrylAc and CryllAb

d)

Nif genes

44.

Which of the following companies started selling humulin in the year 1983 ?

a)

EliLilly

b)

Genetech

c)

GEAC

d)

None of these

45.

Early detection of a disease is possible by

a)

PCR

b)

gene therapy

c)

recombinant DNA technology and ELISA

d)

Both (a) and (c).

46.

Human insulin is being commercially produced from a transgenic species of

a)

Mycobacterium

b)

Rhizobium

c)

Saccharomyces

d)

Escherichia.

47.

‘Flavr Savr’ variety of tomato which remains fresh for a longer period than normal tomato variety

a)

has high amount of enzyme polygalacturonase

b)

has reduced amount of enzyme polygalacturonase

c)

is a pest resistant variety

d)

is rich in vitamin A and prevents night blindness. ‘

48.

The trigger for the activation of the toxin of Bacillus thuringiensis

a)

acidic pH of stomach

b)

high temperature

c)

Alkaline pH of gut

d)

mechanical action in the insect gut

49.

ADA is an enzyme which is deficient in a genetic disorder SCID.what is the full form of ADA?

a)

Adenosine deoxyaminase

b)

Aspartate deaminase

c)

Arginine deaminase

d)

Adenosine deaminase

50.

What was the purpose of the Human Genome Project

a)

to sequence all of the genes in humans

b)

to find every disease in humans

c)

to name all of the nucleotides in humans

d)

to project all of the human's into a genome

51.

What is the main purpose of gel electrophoresis?

a)

mixing of fragments

b)

separation of fragments

c)

to show conduction of electricity through gel.

52.

EFB stands for

(a)  

53.

Enzyme used to open the cell envelope of a bacteria

a)

cellulase

b)

chitinase

c)

lysozyme

d)

ribozyme

54.
Organisms that have DNA from a different species artificially inserted into their cells are called
a)
bioremediated
b)
transgenic
c)
cultured
d)
a clone
55.
Which biotechnology is used to make exact DNA copies of an organism?
a)
Gel electrophoresis
b)
Genetic Engineering
c)
Inbreeding
d)
Cloning
56.
What information must be known before a gene can be inserted into a bacteria?
a)
DNA sequence of the gene
b)
mRNA sequence of the gene
c)
tRNA sequence of the gene
d)
protein sequence of the gene
57.
After finding the location of a gene, how can a scientist take it out of a cell?
a)
Using a microscope
b)
Using PCR
c)
Using scissors
d)
Using enzymes
58.

Ori stands for (a)  

59.

Which among the two is the first restriction endonuclease?

a)

Hind II

b)

Eco RI

60.

Select the correct answer :

Cohen and Boyer extracted antibiotic resistant gene from _______ and inserted in to _________

a)

Ecoli and Salmonella

b)

Salmonella and Ecoli

c)

Influenza and Ecoli

d)

Ecoli and bacteriophage

61.

The strain of the micro organism can be identified from the third letter in the restriction enzyme.

a)

True

b)

False

62.
Which of the following is a protein that acts as DNA scissors?
a)
plasmid
b)
restriction enzyme
c)
ligase
d)
protein expression
63.
Which is true about plasmids?
a)
Plasmids can be cut at specific sequences called restriction sites.
b)
Plasmids contain a promoter sequence that defines where transcription begins.
c)
Plasmids can contain an antibiotic resistance gene.
d)
All of the statements are true about plasmids.
64.
Which of the following terms describes the process of the uptake of recombinant plasmids into bacteria cells?
a)
rDNA technology
b)
transferrence
c)
transformation
d)
rDNA plasmid
65.

What type of cut is this photo?

a)

Blunt

b)

sticky

66.
An organism that contains DNA from another organism is called:
a)
transatlantic
b)
recombinant
c)
binary
d)
selective species
67.
This picture shows how what is made?
a)
Recombinant DNA
b)
PCR
c)
Gel Electrophorisis
d)
Clones
68.
In which step would the enzyme ligase be used?
a)
A
b)
B
c)
C
d)
D
69.

Which of these is the "gene of interest"?

a)

A

b)

B

c)

C

d)

D

70.
A protein of interest is _____________. 
a)
the entire genome of the organism
b)
a section of DNA (a gene) that codes for a protein
c)
a protein that can be used to treat a disease
d)
a protein that has an interesting shape