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2025 Biology Benchmark

Total questions: 40

Worksheet time: 1hrs 3mins

Name
Class
Date
1.

What happens to water when it freezes?

a)

It expands and floats, becoming more dense

b)

It expands and floats, becoming less dense

c)

It contracts and sinks, becoming more dense

d)

It contracts and sinks, becoming less dense

2.

______ is the movement of water from high to low concentration across a membrane.

a)

Cohesion

b)

Diffusion

c)

Osmosis

d)

Adhesion

3.
What word describes when water is attracted to other substances?
a)
cohesion
b)
adhesion
c)
capillary action
d)
surface tension
4.

Why is water considered a polar molecule?

a)

The oxygen end is slightly negative and the hydrogen end is slightly positive

b)

The hydrogen end is slightly negative and the oxygen end is slightly positive

c)

The hydrogen and oxygen are covalently bonded

d)

Because it is hydrophobic

5.

water moves up a thin tube

a)

Polarity

b)

Surface tension

c)

Capillary action

d)

Universal solvent

6.

Label 2 shows a...

a)

Hydrogen bond

b)

Covalent bond

7.

Organize the macromolecules according to their elemental composition.

Categorize the following

Carbohydrates

Lipids

Proteins

Nucleic Acids

CHO
CHON
CHONP
8.

enzymes _________ reactions by _________ activation energy.

a)

speed up / raising

b)

speed up / lowering

c)

slow down / raising

d)

slow down / lowering

9.

Click on the model that correctly depicts the water molecule.

10.

Match each property of water to its description.

a)

A water molecules has regions of positive (+) and negative (-) charge.

1.

Polarity

b)

Water absorbs a lot of heat energy before its temperature rises.

2.

High specific heat

c)

Water molecules stick to other water molecules

3.

Cohesion

d)

Water molecules stick to other substances or surfaces

4.

Adhesion

e)

Water moves up a thin tube, like the tissue of a plant (xylem)

5.

Capillary action

11.

Match each scenario to its most useful macromolecule.

a)

You are about to run a mile.

1.

Carbohydrate

b)

You need enzymes to digest food quickly

2.

Protein

c)

You are a hibernating animal

3.

Lipid

d)

You pass a gene on to your offspring.

4.

Nucleic acid

12.

Match each macromolecule to its structure.

a)

carbon rings called monosaccharides

1.

Carbohydrate

b)

3D folded chains of amino acids

2.

Protein

c)

long, straight chains of hydrocarbon fatty acids

3.

Lipid

d)

chains of nucleotides, usually in a helix shape

4.

Nucleic acid

13.

Match each macromolecule to its function keywords.

a)

insulation, long term energy

1.

lipid

b)

fast, short-term energy

2.

carbohydrate

c)

genetic

3.

nucleic acid

d)

enzymes, muscle, immune system

4.

protein

14.

The cell membrane is composed primarily of which macromolecules?

a)

Lipids

b)

Proteins

c)

Carbohydrates

d)

Nucleic acids

15.
Which is NOT a part of the Cell Theory?
a)

Cells are the smallest unit of life

b)

Cells arise from other living cells

c)

All cells store DNA in a nucleus

d)

All living things are made of one or more cells

16.

This organelle takes food and turns it into ENERGY for plant and animal cells during CELL RESPIRATION.

a)
Chloroplast
b)
Mitochondria
c)
Lysosome
d)
Ribosome
17.

You can see in this picture that these cells have chloroplasts and rigid cell walls. What type of cells are they?

a)
animal cells
b)
plant cells
18.

Match each organelle to its key word.

a)

mitochondria

1.

energy (ATP)

b)

golgi

2.

packages

c)

nucleus

3.

control

d)

ribosomes

4.

proteins

e)

vacuole

5.

storage

19.

Match each organelle to its key word(s).

a)

chloroplast

1.

photosynthesis

b)

ER

2.

transport

c)

vesicle

3.

small package

d)

lysosome

4.

digestive enzymes

e)

cell wall

5.

structural support

20.

In which diagram would water move INTO of the cell?

(black dots = solute)

a)

b)

c)

21.

This diagram shows the _________ transport of solute.

a)

Active

b)

Passive

22.

Going into the cell, the free movement of ANY molecule from HIGH → LOW concentration is called...

(hint: it does NOT require energy)

a)

osmosis

b)

active transport

c)

diffusion

d)

exocytosis

23.

Sort as either Prokaryotic or Eukaryotic

Categorize the following

NO nucleus

Nucleus

Bacteria

Plants, animals, fungi, protists

Free-floating DNA in cytoplasm

Membrane-bound organelles

May have flagella, pili

May have mitochondria, chloroplasts, lysosomes, and more

Small, simple

Large, complex

ALL unicellular

MOSTLY multicellular

Prokaryotic
Eukaryotic
24.

Under a microscope, you see a large cell with a nucleus and a cell wall.

What type of cell is this?

a)

Prokaryotic - Plant

b)

Eukaryotic - Plant

c)

Prokaryotic - Animal

d)

Eukaryotic - Animal

25.

Organize these options into the right categories

Categorize the following

A testable prediction or "educated guess"

"If....Then..."

A WHY explanation backed by a lot of evidence

A WHAT explanation that uses MATH (formulas, equations)

Hypothesis
Theory
Law
26.

Which of these membrane processes requires energy?

a)

facilitated diffusion

b)

passive transport

c)

active transport

d)

protein assisted diffusion

27.

Reorder the phases of mitosis.

a)

b)

c)

d)

1)
2)
3)
4)
28.

Match each phase of the cell cycle to its description.

a)

Interphase

1.

Copy DNA

Grow

b)

Prophase

2.

DNA packed into chromosomes

c)

Metaphase

3.

Chromosomes line up in the middle

d)

Anaphase

4.

Chromatids pulled apart

e)

Telophase

5.

Two nuclei form

29.

The reason organisms grow, and can repair damaged tissue is...

a)

mitosis

b)

photosynthesis

c)

cell respiration

d)

osmosis

30.

Sort options into 1 of the 2 types of cell respiration.

Categorize the following

OXYGEN

NO OXYGEN

MORE ATP made

LESS ATP made

Aerobic
Anaerobic
31.

Drag & drop items to complete the input/output cycle for photosynthesis and cell respiration.

Not all labels will be used.

32.

The purpose of photosynthesis is to make...

a)

b)

c)

d)

33.

The purpose of cell respiration is to make...

a)

b)

c)

d)

34.

Photosynthesis and cell respiration both ​ (a)   energy.

Photosynthesis transforms ​ (b)   energy from the sun into stored ​ (c)   energy (glucose sugar).

Cell respiration transforms chemical energy into ​ (d)   energy (ATP).

Choose from the below words
transform
chemical
radiant
create
destroy
cellular
35.

Label the DNA molecule.

Not all answers will be used.

36.

Categorize each as either DNA or RNA.

Categorize the following

Double-stranded

Single-stranded

A-T, C-G

A-U, C-G

Deoxyribose sugar

Ribose sugar

DNA
RNA
37.

Label the diagram with the 2 steps of protein synthesis.

38.

Which suspect has DNA that matches the blood stain?

a)

1

b)

2

c)

3

39.

Match the DNA code to its mRNA code.

a)

ATACGA

1.

UAUGCU

b)

ACGGTA

2.

UGCCAU

c)

TAAGTT

3.

AUUCAA

40.

What does the question mark represent?

a)

DNA

b)

Protein

c)

mRNA

d)

Chromosomes