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WorksheetsVitamin Assessment Quiz
Total questions: 155
Worksheet time: 1hrs 18mins
Which of the following is a static test for assessing nutrient status?
Methionine load test
Plasma retinol concentration
Dark adaptation test
Rhodopsin response test
The Relative Dose-Response (RDR) test for vitamin A assesses:
Total vitamin A in diet
Plasma vitamin A changes after supplementation
Retinol-binding protein function
Liver enzyme levels
What is the formula for calculating the RDR value?
(Vit A0 – Vit A5) / Vit A5 × 100
Vit A5 / Vit A0 × 100
(Vit A5 – Vit A0) / Vit A5 × 100
Vit A5 × Vit A0 / 100
Which test is considered the gold standard for Vitamin A status?
Serum retinol test
Retinol-binding protein assay
Conjunctival impression cytology
Liver biopsy
What is the main circulating form of Vitamin D used to assess status?
1,25-dihydroxyvitamin D
Ergocalciferol
25-hydroxyvitamin D
Retinyl palmitate
Vitamin D deficiency in adults can lead to:
Scurvy
Osteomalacia
Rickets
Pellagra
What is the clinical deficiency disease for Vitamin C?
Pellagra
Beriberi
Rickets
Scurvy
A serum Vitamin C concentration below 11.4 μmol/L indicates:
Toxicity
Normal range
Deficiency
Infection
What is the major urinary metabolite used to assess recent Vitamin B6 intake?
PLP
4-pyridoxic acid
Pyridoxine
Homocysteine
Which is the most frequently used biochemical indicator of Vitamin B6 status?
Urinary PL
Methionine test
Plasma PLP
Plasma pyridoxine
Which of the following reflects long-term folate status?
Serum folate
Urinary folate
RBC folate
Homocysteine
A low serum folate concentration is defined as:
<2 ng/mL
<100 ng/mL
<5 μmol/L
<11.4 μmol/L
What is a direct marker of Vitamin B12 status?
Total homocysteine
Serum MMA
Total serum cobalamin
Plasma folate
Elevated methylmalonic acid (MMA) in serum may indicate:
Folate deficiency
Vitamin A toxicity
Vitamin B12 deficiency
Iron overload
A limitation of MMA and tHcy testing is that levels may also be elevated in:
Liver cancer
Renal dysfunction
Obesity
High protein diets
ALT is commonly elevated in which condition?
Asthma
Liver disease
Bone fracture
Dehydration
Which enzyme is elevated in both liver and muscle damage?
ALT
ALP
AST
LDH
ALP levels are normally higher in:
Elderly males
Adolescents during skeletal growth
Postmenopausal women
Diabetic patients
What is the primary function of albumin in blood?
Bone formation
Oxygen transport
Maintenance of osmotic pressure
Hormone synthesis
A serum bilirubin >2 mg/dL indicates:
Hypocalcemia
Hepatitis
Jaundice
Kidney failure
Elevated unconjugated bilirubin usually results from:
Bile duct obstruction
Liver cirrhosis
Hemolysis
Alcoholism
What is the primary waste product used to assess kidney function?
Bilirubin
Uric acid
Blood urea nitrogen (BUN)
Ammonia
Azotemia refers to elevated levels of:
Creatinine
Sodium
BUN
Albumin
What is the normal range of serum calcium?
3.5–5.0 mg/dL
4.5–5.6 mg/dL
9.0–10.5 mg/dL
10.5–13.0 mg/dL
Hyperkalemia is commonly caused by:
Dehydration
Excess dietary intake
Renal failure
Anemia
The major extracellular cation in the body is:
Potassium
Magnesium
Sodium
Chloride
Which electrolyte often mirrors changes in sodium and is important in acid-base balance?
Potassium
Chloride
Calcium
Phosphorus
A normal phosphorus level in adults is:
1.5–2.5 mg/dL
3.0–4.5 mg/dL
5.5–7.0 mg/dL
8.0–10.0 mg/dL
Which blood component is elevated in myocardial infarction and liver disease?
AST
LDH
ALT
ALP
Which test is commonly used in diabetes diagnosis?
Creatinine
Hemoglobin
Fasting plasma glucose (FPG)
LDL cholesterol
Which test is used to assess epithelial changes in Vitamin A deficiency?
Liver biopsy
Dark adaptation test
Conjunctival impression cytology
Serum retinol-binding protein
Which vitamin is essential for collagen formation and iron absorption?
Vitamin D
Vitamin C
Vitamin B12
Vitamin A
Which form of vitamin B6 is most abundant in plasma and used for assessment?
Pyridoxamine
Pyridoxine
Pyridoxal 5′-phosphate (PLP)
Pyridoxal
What is the main disadvantage of using RBC PLP for vitamin B6 status?
Too expensive
Invasive technique
Interference by hemoglobin binding
Easily altered by hydration status
What is the normal lifespan of erythrocytes, affecting RBC folate measurements?
60 days
100 days
120 days
180 days
What is the function of folate in DNA synthesis?
Methyl group donor for proteins
Iron carrier
Synthesis of purines and pyrimidines
Cholesterol conversion
Which vitamin is mainly obtained from animal-based foods or fortified products?
Vitamin C
Vitamin B12
Vitamin B6
Folate
What is the major circulating protein that transports Vitamin A in plasma?
Albumin
Hemoglobin
Transferrin
Retinol-binding protein
In MRDR test, which compound is given orally?
Retinyl palmitate
Dehydroretinol
Beta-carotene
Isotopic retinol
In the dark adaptation test, which photopigment is directly involved?
Melanin
Rhodopsin
Retinaldehyde
Carotene
A result of low plasma PLP may occur due to all the following EXCEPT:
Coronary heart disease
Pregnancy
Asthma
High protein diet
Which condition is associated with low RBC folate?
Hyperparathyroidism
Megaloblastic anemia
Osteoporosis
Hypocalcemia
What method is used for minimally invasive total body vitamin A assessment?
Plasma retinol test
Retinol isotope dilution
Serum RBP
Conjunctival test
Which enzyme is NOT primarily associated with liver function?
ALT
AST
LDH
Creatine kinase
A high BUN level can be caused by:
Excess water intake
Liver disease
Dehydration
Hypokalemia
Which of the following is a product of protein metabolism?
Creatinine
Uric acid
Urea
Homocysteine
An increased serum creatinine level is a sign of:
Good hydration
Liver disease
Renal impairment
Bile duct obstruction
Which condition may result in hypokalemia?
Addison’s disease
Diuretic use
Kidney failure
Hemolysis
Which ion is essential in nerve impulse transmission?
Calcium
Phosphate
Potassium
Chloride
Which electrolyte imbalance is seen in vomiting and diarrhea?
Hypernatremia
Hyponatremia
Hyperkalemia
Hypercalcemia
A low serum chloride (hypochloremia) may indicate:
Metabolic acidosis
Dehydration
Alkalosis
High blood pressure
Hyperphosphatemia is commonly seen in:
Vitamin D deficiency
Rickets
Renal failure
Pregnancy
Which of these markers is influenced by kidney function and may lead to false results in elderly?
Plasma PLP
MMA and tHcy
ALT and AST
Total cholesterol
Which condition would most likely result in elevated ALT and AST?
Dehydration
Viral hepatitis
Iron deficiency
Hypocalcemia
ALT is found in greatest concentrations in which organ?
Pancreas
Heart
Liver
Muscle
ALP can be elevated in all EXCEPT:
Liver cirrhosis
Pregnancy
Bone growth
Diabetes
A low serum albumin level may indicate:
Iron deficiency
Malnutrition
Elevated calcium
Overhydration
What condition is associated with elevated conjugated (direct) bilirubin?
Bile duct obstruction
Hemolytic anemia
Fasting
Bone fracture
Unconjugated bilirubin is bound to:
Urea
Globulin
Albumin
Calcium
Elevated BUN and creatinine are both indicative of:
Liver disease
Pancreatitis
Renal dysfunction
Rickets
What is the main difference between BUN and creatinine?
BUN is more specific
Creatinine is more stable
Creatinine fluctuates with hydration
BUN is not affected by protein intake
Which of the following can lead to decreased BUN?
High protein diet
Liver failure
Dehydration
Obstruction of the urinary tract
Which condition is associated with elevated serum calcium?
Hypoparathyroidism
Rickets
Hyperparathyroidism
Iron deficiency anemia
Hypokalemia may be caused by all EXCEPT:
Vomiting
Diuretics
Diarrhea
Kidney failure
What condition is most associated with hyperkalemia?
Dehydration
Excess potassium intake
Renal failure
Fasting
Hyponatremia may occur in:
Excess sodium intake
Diarrhea
Fever
Obesity
What does serum CO₂ reflect in blood chemistry tests?
Oxygenation
Base excess
Acid-base balance
Electrolyte levels
Elevated serum phosphorus may be caused by:
Overhydration
Chronic renal failure
Vitamin D deficiency
Diuretics
Which mineral is closely regulated with phosphorus in serum levels?
Magnesium
Calcium
Sodium
Iron
LDH levels are usually elevated during:
Liver enzyme suppression
Dehydration
Tissue breakdown
Muscle relaxation
Which is NOT part of the typical blood chemistry panel?
Creatinine
LDH
Glucose
Vitamin K
Which test is commonly used to screen for cardiovascular risk?
AST
HDL/LDL ratio
ALT
Creatinine
A high fasting triglyceride level can result from all EXCEPT:
Excess alcohol
High carbohydrate diet
Obesity
Iron overload
What is the most commonly used serum protein in assessing protein status?
Prealbumin
Albumin
Retinol-binding protein
Transferrin
The formula for calculating nitrogen balance is:
UUN - PRO / 6.25
PRO x 6.25 - UUN
(PRO / 6.25) - (UUN + 4)
(UUN + 4) / PRO x 6.25
Which indicator is most sensitive for detecting early iron deficiency?
Hemoglobin
Serum ferritin
Hematocrit
Serum iron
Which is NOT a limitation of biochemical tests?
Can reflect long-term intake only
Influenced by non-nutritional factors
Can be technically challenging
Some tests are nonspecific
Creatinine-height index (CHI) is used to assess:
Iron absorption
Calcium metabolism
Muscle mass depletion
Iodine status
What fraction of serum calcium is physiologically active?
Protein-bound
Ionized
Complexed
Bound to albumin
What is the most feasible current approach to assess calcium status?
Serum calcium
Bone mineral content
Urinary calcium
Plasma phosphate
In protein-energy malnutrition, CHI value below 40% indicates:
Mild depletion
Normal status
Severe depletion
Moderate depletion
Which marker increases during early iron-deficient erythropoiesis?
Serum ferritin
Hemoglobin
Soluble transferrin receptor (sTfR)
Hematocrit
Plasma zinc concentration is:
A reliable indicator of zinc status
Affected by stress and infection
Completely unaffected by hormones
Best used after supplementation only
Iron deficiency anemia is associated with:
Increased serum ferritin
Increased MCV
Decreased transferrin saturation
Normal hemoglobin levels
What does the erythrocyte protoporphyrin level indicate?
Protein intake
Early iodine deficiency
Impaired heme synthesis
Serum calcium status
Which urinary biomarker is most widely used to assess iodine status?
Creatinine
UUN
Urinary Iodine (UI)
TSH
Which serum protein has the shortest half-life?
Albumin
Transferrin
Prealbumin
Ferritin
What is the cause of hypercalcemia?
Hypoparathyroidism
Vitamin C deficiency
Hyperthyroidism
Iron deficiency
Which test best indicates iron overload?
Hemoglobin
TIBC
Serum ferritin
MCH
Zinc in hair reflects:
Short-term dietary changes
Diurnal variations
Long-term nutritional status
Serum iron level
Which is the most reliable model to assess iron status in inflammatory conditions?
MCV model
Ferritin model
Body iron model
CHI
Hematocrit is a measure of:
Oxygen capacity
Total blood calcium
% of RBCs in whole blood
Zinc concentration
What could lead to falsely low zinc levels?
Hair growth
Fasting
Inflammation
Supplementation
What is NOT a function of zinc?
Wound healing
Hormone production
Enzyme component
Bone mineralization
What is the main reason for calcium status not being accurately assessed biochemically?
Limited intake
Stable serum levels due to homeostasis
Low absorption
Excess losses
Which hormone is most sensitive to neonatal iodine status?
TSH
T4
Thyroglobulin
Insulin
What does MCV stand for?
Mean Cell Volume
Mean Corpuscular Volume
Maximum Cell Volume
Membrane Cellular Volume
Which protein binds most of the iron in serum?
Albumin
Hemoglobin
Transferrin
Ferritin
Which marker of protein status is most affected by hydration?
Retinol-binding protein
Albumin
CHI
Creatinine excretion
A patient has low serum zinc and high metallothionein. What does it suggest?
Zinc deficiency
Acute infection response
Iodine overload
Poor protein intake
The ratio of serum iron to TIBC is called:
Ferritin index
Iron index
Transferrin saturation
Iron capacity ratio
What is the function of metallothionein in zinc assessment?
Reflects long-term zinc stores
Unreliable for all assessments
Helps differentiate between redistribution and deficiency
Increases with true deficiency only
Urinary zinc analysis is less preferred because:
It is extremely accurate
Not influenced by diseases
It’s only possible with children
Influenced by non-nutritional factors and difficult collection
Which component is essential for thyroid hormone synthesis?
Zinc
Iron
Calcium
Iodine
The creatinine-height index (CHI) compares:
Protein intake vs. excretion
Measured vs. expected urinary creatinine
Serum creatinine vs. plasma creatinine
Dietary protein vs. nitrogen output
Which fraction of serum calcium is inactive?
Ionized
Complexed
Protein-bound
Free
One key drawback of hair zinc measurement is:
It is invasive
It fluctuates daily
Easily contaminated
Requires fasting
Which hormone regulates serum calcium homeostasis?
Insulin
Parathyroid hormone
Thyroxine
Cortisol
Soluble transferrin receptor (sTfR) levels rise in:
Iodine excess
Early iron deficiency
Calcium deficiency
Zinc deficiency
In iron depletion's first stage, which marker decreases?
Hemoglobin
MCV
Serum ferritin
sTfR
The best sample to estimate recent iodine intake in individuals is:
Hair
Saliva
24-hour urine
Feces
Retinol-binding protein is used to assess:
Zinc status
Iodine status
Protein-energy status
Bone mineral density
What condition may cause hypocalcemia?
Hyperthyroidism
Hypervitaminosis D
Hypoparathyroidism
Excess protein intake
Which of the following is a functional indicator of nutrient deficiency?
Serum iron
Plasma calcium
Dark adaptation test
UUN
CHI of 75% indicates:
Normal muscle mass
Moderate depletion
Severe depletion
Mild depletion
What is the primary method of iron loss in premenopausal women?
Urinary excretion
Menstruation
Sweat loss
Hemorrhoids
Metallothionein helps interpret:
Calcium excretion
Plasma zinc levels during stress
TSH in neonates
Urinary iodine
MCV reflects:
Plasma protein concentration
Red blood cell size
Bone mineralization
Zinc-binding capacity
Hemoglobin is not a good indicator of early iron deficiency because:
It is unreliable
It’s too sensitive
It only drops in later stages
It is affected by calcium
Which of the following is LEAST invasive for nutrient assessment?
Plasma zinc
24-hour urine
Hair zinc
Liver biopsy
A person with high protein intake and low phosphate intake may have:
Increased calcium excretion
Decreased zinc excretion
Increased iodine retention
Decreased serum calcium
A major cause of iron overload is:
Poor iron absorption
Multiple blood transfusions
Low dietary fiber
Overuse of calcium
Which value is calculated by dividing hemoglobin by RBC count?
MCV
MCH
TIBC
CHI
Which specimen type is least commonly used in routine biochemical assessment?
Blood
Urine
Saliva
Feces
What is the main protein used for short-term assessment of protein status?
Albumin
Prealbumin
Transferrin
Retinol-binding protein
Which condition is associated with zinc deficiency?
Hyperthyroidism
Hypoparathyroidism
Dwarfism
Osteoporosis
What factor increases the risk of iron deficiency during adolescence?
Reduced blood volume
Increased dietary fiber
Rapid growth
Calcium overload
Which test is LEAST useful in diagnosing calcium deficiency?
Bone mineral content
Serum calcium
Urinary calcium
Calcium isotope tracer
Which component measures the amount of iron bound to transferrin?
TIBC
Hematocrit
Serum iron
CHI
What does MCH represent?
Total RBC volume
Hemoglobin per red cell
Calcium per liter
Transferrin concentration
Why is plasma zinc not reliable during acute illness?
Increases rapidly
Not absorbed in illness
Levels fall due to redistribution
Zinc is destroyed by fever
Serum ferritin is a marker of:
Iron stores
Iron excretion
Zinc absorption
Hemoglobin transport
What is the primary function of urinary urea nitrogen (UUN) in assessment?
Iron absorption
Protein catabolism estimation
Calcium loss
Iodine excretion
Transferrin saturation is calculated using:
Serum ferritin / Hemoglobin
MCH x MCV
Serum iron / TIBC
Hematocrit / Serum iron
Zinc levels in hair can be affected by:
TSH
Hair color and age
Serum iodine
Ferritin
The test measuring total iron-binding capacity provides an estimate of:
Hemoglobin concentration
Transferrin availability
Ferritin stores
Plasma zinc
Which test is most useful for assessing population iodine status over time?
TIBC
Serum ferritin
Goiter rate
CHI
What value is typically low in microcytic anemia?
MCV
TIBC
UUN
Serum calcium
What increases erythrocyte protoporphyrin?
Excess calcium
Iron overload
Iron deficiency
Vitamin A deficiency
A normal serum ferritin but low hemoglobin and low MCV may indicate:
Acute infection
Zinc excess
Iron overload
Early-stage protein malnutrition
Which is NOT a limitation of using urinary zinc for status assessment?
Affected by disease
Hard to collect
Reflects long-term intake
Inconsistent excretion
What is the ideal biomarker for diagnosing iron deficiency in inflammation?
CHI
Serum ferritin
sTfR
Albumin
Which of the following decreases during severe protein-energy malnutrition?
UUN
Transferrin
Plasma zinc
All of the above
Which of the following contributes the most to total energy expenditure?
Physical activity
Thermic effect of food
Basal metabolism
Adaptive thermogenesis
What does a bomb calorimeter measure?
Rate of food digestion
Absorption of calories
Heat released from burning food
Physical energy during activity
Which is NOT a component of energy expenditure?
Thermic effect of food
Basal metabolism
Physical activity
Water consumption
What is the typical percentage of energy used for the thermic effect of food?
5%
10%
25%
50%
Which source is used in Malaysia to obtain nutrient data for foods?
USDA Food Composition
EFSA Database
Malaysian Food Composition Database (MYFCD)
FAO Nutrient Atlas
Which of the following is considered the gold standard method for measuring total energy expenditure?
Direct calorimetry
Doubly labeled water
Harris-Benedict Equation
Quick Method
When energy intake equals energy output, the person is said to be in:
Positive energy balance
Negative energy balance
Energy surplus
Energy balance
The Quick Method of estimating energy needs typically uses:
Detailed food diary
Body weight and activity factor
Basal metabolic rate only
Doubly labeled water test
If a person’s TEI is 2000 kcal/day and 30% is from fat, how many kcal comes from fat?
400 kcal
500 kcal
600 kcal
700 kcal
Which macronutrient provides the highest kcal per gram?
Carbohydrate
Protein
Fat
Water
Which of the following methods measures heat released by the body to determine energy expenditure?
Indirect calorimetry
Direct calorimetry
Respiratory quotient
Doubly labeled water
The basal metabolic rate (BMR) is typically measured:
After exercise and a full meal
While standing and active
After an overnight fast and rest
During intense physical activity
