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Worksheets5th session
Total questions: 71
Worksheet time: 37mins
What is the most logical sequence of steps for splicing foreign DNA into a plasmid and What is the most logical sequence of steps for splicinWhat is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I. Transform bacteria with a recombinant DNA molecule.
II. Cut the plasmid DNA using restriction enzymes (endonucleases).
III. Extract plasmid DNA from bacterial cells.
IV. Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.
V. Use ligase to seal plasmid DNA to non-plasmid DNA.
II, III, V, IV, I
III, II, IV, V, I
III, IV, V, I, II
IV, V, I, II, III
Why are yeast cells frequently used as hosts for cloning?
They easily form colonies.
They can remove exons from mRNA.
They do not have plasmids.
They are eukaryotic cells.
Which of the following processes is most like the formation of identical twins?
cell cloning
therapeutic cloning
use of adult stem cells
organismal cloning
In DNA technology, the term vector can refer to
the enzyme that cuts DNA into restriction fragments
the sticky end of a DNA fragment.
a SNP marker.
a plasmid used to transfer DNA into a living cell.
Which of the following tools of DNA technology is incorrectly paired with its use?
electrophoresis- separation of DNA fragments
DNA ligase-cutting DNA, creating sticky ends of restriction fragments
DNA polymerase- polymerase chain reaction to amplify sections of DNA
reverse transcriptase- production of cDNA from mRNA
6. Bioinformatics includes
I. using computer programs to align DNA sequences
II. creating recombinant DNA from separate species
III. developing computer-based tools for genome analysis
IV. using mathematical tools to make sense of biological systems
I and II
II and III
II and IV
I, III, and IV
Which of the following can be duplicated in a genome?
only DNA sequences
only entire sets of chromosomes
only entire chromosomes
DNA sequences, chromosomes, or sets of chromosomes
The Linnaeus classification system grouped organisms by
linear hierarchy of the scala naturae
increasingly more general categories
increasingly more complex categories
environmental location
Darwin used the phrase "descent with modification" to explain
unity of life
descent of all organisms from a single, ancient ancestor.
that habitat differences stimulate change in organisms
evolution of the unity and diversity of life
Which of the following statements describe evolution?
Individuals evolve in response to their environment.
The match between organism and their environment decreases.
Natural selection chooses the most popular trait.
Population evolve in response to their environment.
Arrange the following in order from most general to most specific.
1. natural selection
2. microevolution
3. intrasexual selection
4. evolution
5. sexual selection
4, 1, 2, 3, 5
4, 2, 1, 3, 5
4, 2, 1, 5, 3
1, 4, 2, 5, 3
Natural selection changes allele frequencies because some survive and reproduce better than others.
alleles
loci
species
individuals
What does the biological species concept use as the primary criterion for determining species boundaries?
geographic isolation
niche differences
gene flow
morphological similarity
The largest unit within which gene flow can readily occur is.
a population
a species
the entire range of a genus
the hybrid zone
What is the term given to the theory which put forth the idea that living organisms could arise by incubating nonliving components?
spontaneous generation
natural selection
evolution
preformation
Who was the Augustinian monk that conducted a decade of experiments on the garden pea, eventually showing that traits are passed from parents to offspring in predictable ways?
Francis Crick
Alfred Wallace
Hippocrates
Gregor Mendel
Genetics is defined as the branch of biology associated with.
heredity and variation
mutation and recession
transcription and translation
diploid and haploid
The observable feature of an organism is referred to as
genotype
phenotype
prototype
karyotype
Genetic information contained in DNA that encodes for an amino acid is referred to as an
allele
trait
nucleotide
codon
A primary discovery to generate recombinant DNA molecules was the use of
Xray diffraction
restriction enzymes
microscopes
bioinformatic
is an organism produced by biotechnology that involves the transfer of hereditary traits across species
transgenic organism
mutant
clone
vecto
The study of the structure, function, and evolution of genes and genomes is referred to as
genomics
proteomics
bioinformatics
genetics
Organisms that are well understood from a scientific standpoint and are often used in basic biological research are often called
clones
vectors
recombinant DNA technology
model organisms
DNA, as genetic material/transforming principle was experimently proved by which of the following scientists?
Friedrick Meischer and Griffith
Watson and Crick
Rosalin and Willkins
Avery Mcleod and Mc Carty
The breaking of hydrogen bonds in a dna molecule when subjected to high temperature is known as
Renaturation
Denaturation
Annealing
Ionisation
Which term describes DNA replication?
Conservative
Semiconservative
Dispersive
None of these
RNA is synthesized on a DNA template in a process called, which utilizes the enzyme
translation, RNA polymerase
transcription, DNA polymerase
transcription, RNA polymerase
replication, DNA polymerase
Type of mutation in which a pyrimidine is substituted for a purine
Transition
Transversion
Frameshift
Conversion
Inversion
A base change that changes a codon for an amino acid to a stop codon
Missense
Silent
Nonsense
Synonymous
Frameshift
An enzyme that recognizes a specific (palindromic) sequence and cuts within a DNA molecule is called a(n):
exonuclease
methylase
modification enzyme
restriction endonuclease
Which is the correct order, from smallest to largest number of base pairs?
plasmid, transposon, chromosomal DNA
chromosomal DNA, transposon, plasmid
transposon, plasmid, chromosomal DNA
plasmid, chromosomal DNA transposon
Which of the following people conducted the experiments that demonstrated that DNA is the genetic material of bacteriophages?
Hershey and Chase
Pauling
Franklin
Watson and Crick
Which of the following options best depicts the flow of information when a gene directs the synthesis of a cellular component?
DNA → RNA →protein
protein → RNA → DNA
DNA → tRNA →mRNA → protein
RNA → DNA → RNA→ protein
marks the end of a gene and causes transcription to stop.
A terminator
Methionine
RNA ligase
RNA polymerase
Approximately what percentage of the human genome is identical to that of a chimpanzee?
98.8%
92.0%
62.3%
50.0%
Segments of eukaryotic DNA that can move or be copied from one site to another in the genome are called
vectors.
transposable elements.
exons.
plasmids
What is the current standard tool used for DNA profiling by forensic scientists?
DNA microarrays
Gel electrophoresis
STR analysis
PCR
What is the preferred name of the technique used to determine if DNA comes from a particular individual?
DNA profiling
DNA fingerprinting
DNA scrutiny
DNA outline
Golden rice is golden in color because it is rich in
vitamin C.
beta-carotene.
chromium picolinate.
vitamin A.
A vaccine works by
stimulating the immune system.
inhibiting bacterial replication.
inhibiting viral replication.
preventing the translation of mRNA.
The only recombinant cells that can correctly attach sugars to proteins to form glycoprotein products are
yeast cells.
mammalian cells.
E. coli cells.
algal cells.
The type of recombinant bacteria most often used to mass-produce genes is
Escherichia coli.
Pseudomonas aeruginosa.
Agrobacterium tumefaciens.
Saccharomyces cerevisiae.
A collection of DNA fragments obtained from the genome of one organism, inserted by recombinant DNA techniques into the genome of a host organism (one fragment per host genome), and maintained there is called a
DNA file.
genomic library.
DNA collection.
gene bank.
are a major source of restriction enzymes.
Archaea
Parietal cells
Bacteria
Chief cells
The production of multiple identical copies of gene-sized pieces of DNA defines
tissue culturing.
clonal selection.
plasmid transformation.
gene cloning.
When DNA from two sources is combined into one single piece of DNA, it is known as
cloned DNA.
a vector.
a plasmid.
recombinant DNA.
Biotechnology
has been around since the dawn of civilization.
is generally considered more harmful than valuable to society.
is a modern scientific discipline that has existed for only a few decades.
is strictly concerned with the manipulation of DNA.
The carcinogen known to cause the most cases and types of cancer is
alcohol.
ultraviolet light.
tobacco.
plutonium
A gene that can cause cancer when present in a single copy in a cell is called a(n)
enhancer gene.
proto-oncogene.
carcinogen.
oncogene.
The use of cloning to produce special embryonic stem cells is called
reproductive cloning.
regenerative cloning.
transplantational cloning.
therapeutic cloning
The coding regions of a gene (the portions that are expressed as polypeptide sequences) are called
redundant coding sections.
exons.
introns.
proto-oncogenes
The basis of cellular differentiation is
selective gene expression.
mutation.
cloning.
the operon
In a prokaryote, a group of genes with related functions, along with their associated control sequences, defines
an operon.
an allele.
a transposon.
a locus
Any change in the nucleotide sequence of DNA is called
a mutagen.
a mutation.
a base substitution.
an anticodon
Human insulin is being commercially produced from a transgenic species of
Rhizobium
Saccharomyces
Escherichia
Mycobacterium
Scientists at the biotechnology company Genentech created the first recombinant DNA product for use in humans. Approved by the Food and Drug Administration in 1982, this product was
a gene chip
Chymosin
Insulin
human growth hormone
Which area of biotechnology primarily involves using living organisms to process, degrade, and clean up environmental pollution?
Genomics
Bioinformatics
Bioremediation
Marine biotechnology
Studying the entire complement of DNA in an organism's cells is known as
genomics
Bioinformatics
bioremediation
nanotechnology
is customized medicine based on a person's genetics.
Genomics
Bioinformatics
Bioremediation
Pharmacogenomics
involves farm-raising finfish, shellfish, algae, and other aquatic organisms
Biopharming
Aquaculture
Pharmacogenomics
Bioremediation
is the use of normal genes or the replacement of diseased genes for treating human genetic disorders
Genetics
Genomics
Pharmacogenomics
Gene therapy
Selective breeding involves
genetic engineering of animals and plants to improve growth characteristics
mating organisms with desirable characteristics
the use of fermentation to produce biotechnology products
combining sperm and egg cells from different species to produce hybrid organisms
is the use of living organisms or their products to clean up the environment, improve human health, and provide more nutritious and disease-free food
Bioremediation
Biotechnology
Genetic engineering
Biodegradation
Which of the following is the application of biotechnology to industrial processes
Microbial biotechnology
Animal biotechnology
Forensic biotechnology
Medical biotechnology
What three locations account for approximately 95% of biotechnology countries?
Europe, Japan, and South America
Europe, Japan, and North America
Europe, China, and Canada
Canada, Japan, and South America
What technique was most helpful to Watson and Crick in developing their model for the structure of DNA?
radioactive
X-ray crystallography
transgenic animals
electrophoresis
The main technique involved in agricultural biotechnology is.
Tissue culture
Transformation
Plant Breeding
DNA replication
The correct order of steps in PCR is
Extension, Denaturation, Annealing
Annealing, Extension, Denaturation
Denaturation, Annealing, Extension
Extension, Cutting, Denaturation
Which branch of biotechnology focuses on medical and pharmaceutical applications like vaccines and antibiotics?
Green biotechnology
White biotechnology
Red biotechnology
Blue biotechnology
Which of the following techniques is used to introduce foreign DNA into a bacterial cell?
Transformation
Transcription
Translation
Translocation
Which of the following best describes CRISPR-Cas9 technology?
A DNA replication tool
A method for DNA fingerprinting
A gene-editing system
A protein synthesis mechanism
