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htmle-draft8

Total questions: 132

Worksheet time: 1hrs 6mins

Name
Class
Date
1.
Eosin Y
a)
Red
b)
Yellow
c)
Green
d)
Blue
2.
What is the mordant of Heidenhain's Hematoxylin?
a)
Ferric Ammonium Chloride
b)
Mercuric Chloride
c)
Iron salts
d)
Ferric Ammonium Sulfate
e)
Alcoholic Iodine solution
3.
What is the mordant of Weigert's Hematoxylin?
a)
Ferric Ammonium Chloride
b)
Mercuric Chloride
c)
Iron salts
d)
Ferric Ammonium Sulfate
e)
Alcoholic Iodine solution
4.
What ripens Harris Hematoxylin?
a)
Ferric Ammonium Chloride
b)
Mercuric Chloride
c)
Iron salts
d)
Ferric Ammonium Sulfate
e)
Alcoholic Iodine solution
5.
What ripens Ehrlich's Hematoxylin?
a)
Mercuric Chloride
b)
Iron salts
c)
Ferric Ammonium Sulfate
d)
Alcoholic Iodine solution
e)
Sodium Iodate
6.
What ripens Cole's Hematoxylin?
a)
Mercuric Chloride
b)
Iron salts
c)
Ferric Ammonium Sulfate
d)
Alcoholic Iodine solution
e)
Sodium Iodate
7.
used as ripening agents and oxidizing agents
a)
Mercuric Chloride
b)
Iron salts
c)
Ferric Ammonium Sulfate
d)
Alcoholic Iodine solution
e)
Sodium Iodate
8.
Neutral red
a)
Red
b)
Yellow
c)
Green
d)
Blue
9.
Safranin O
a)
Red
b)
Yellow
c)
Green
d)
Blue
10.
Carmine and hematoxylin
a)
Red
b)
Yellow
c)
Green
d)
Blue
11.
Methylene
a)
Red
b)
Yellow
c)
Green
d)
Blue
12.
Toluidine
a)
Red
b)
Yellow
c)
Green
d)
Blue
13.
Celestine
a)
Red
b)
Yellow
c)
Green
d)
Blue
14.
Eosin B
a)
Red
b)
Yellow
c)
Green
d)
Blue
15.
Phloxine B
a)
Red
b)
Yellow
c)
Green
d)
Blue
16.
Picric acid
a)
Red
b)
Yellow
c)
Green
d)
Blue
17.
Orange G
a)
Red
b)
Yellow
c)
Green
d)
Blue
18.
Rose bengal
a)
Red
b)
Yellow
c)
Green
d)
Blue
19.
Light green >) SF
a)
Red
b)
Yellow
c)
Green
d)
Blue
20.
Lissamine
a)
Red
b)
Yellow
c)
Green
d)
Blue
21.
Eosin Y, Eosin B, Phloxine B
a)
Cytoplasmic stain
b)
Nuclear stain
22.
Picric acid
a)
Cytoplasmic stain
b)
Nuclear stain
23.
Orange G
a)
Cytoplasmic stain
b)
Nuclear stain
24.
Rose bengal
a)
Cytoplasmic stain
b)
Nuclear stain
25.
Light green SF, Lissamine green
a)
Cytoplasmic stain
b)
Nuclear stain
26.
Neutral red
a)
Cytoplasmic stain
b)
Nuclear stain
27.
Safranin O
a)
Cytoplasmic stain
b)
Nuclear stain
28.
Carmine and hematoxylin
a)
Cytoplasmic stain
b)
Nuclear stain
29.
Methylene blue, toluidine blue, celestine blue
a)
Cytoplasmic stain
b)
Nuclear stain
30.
In H&E, Nuclei is
a)
Blue to black
b)
Dark blue
c)
Pale pink
d)
Bright orange - red
31.
In H&E, karyosome is
a)
Blue to black
b)
Dark blue
c)
Pale pink
d)
Bright orange - red
32.
In H&E, cytoplasm is
a)
Blue to black
b)
Dark blue
c)
Pale pink
d)
Bright orange - red
33.
In H&E, RBC, Eosinophilic granules and keratin is
a)
Blue to black
b)
Dark blue
c)
Pale pink
d)
Bright orange - red
34.
IN H&E, Calcium and decalcified bone is
a)
Purplish blue
b)
Pink
c)
Deep pink
35.
IN H&E, Decalcified Bone Matrix, Collagen and Osteoid is
a)
Purplish blue
b)
Pink
c)
Deep pink
36.
IN H&E, muscle fibers is
a)
Purplish blue
b)
Pink
c)
Deep pink
37.
When restaining an old section, slide is immersed in xylene for how long?
a)
6 hours
b)
12 hours
c)
24 hours
d)
48 hours
e)
forever
38.
When restaining an old section,, section is in Xylene for how long in order to remove th remaining balsam and subsequently brought down to water?
a)
5 to 10 minutes
b)
15 minutes
c)
5 minutes only
d)
30 minutes
39.
When restaining an old section,, section is placed in 0.5 Potassium Permanganate solution for how long then rinsed with water?
a)
5 to 10 minutes
b)
15 minutes
c)
5 minutes only
d)
30 minutes
40.
When restaining an old section,, section is Immersed in 5% Oxalic Acid for for how long then rinsed with water?
a)
5 to 10 minutes
b)
15 minutes
c)
5 minutes only
d)
30 minutes
41.
- Cell nuclei, Cytoplasmic inclusions and muscle striations appear black.
a)
Heidenhain's Iron hematoxylin method
b)
Celestine blue-haemalum sequence staining
c)
Mallory's Phloxine methylene blue staining
42.
- an oxazine dye used as an alternative to Iron Hematoxylin nuclear stain
a)
Heidenhain's Iron hematoxylin method
b)
Celestine blue-haemalum sequence staining
c)
Mallory's Phloxine methylene blue staining
43.
__ forms a lake with iron alum, acting as a mordant to bind Hematoxylin
a)
Heidenhain's Iron hematoxylin method
b)
Celestine blue-haemalum sequence staining
c)
Mallory's Phloxine methylene blue staining
44.
- Cell Nuclei appears Blue
a)
Heidenhain's Iron hematoxylin method
b)
Celestine blue-haemalum sequence staining
c)
Mallory's Phloxine methylene blue staining
45.
- originally known as Eosin-Methylene Blue method.
a)
Heidenhain's Iron hematoxylin method
b)
Celestine blue-haemalum sequence staining
c)
Mallory's Phloxine methylene blue staining
46.
– those came from substances taken from Coal Tar. Derived from Hydrocarbon Benzene; known as Aniline Dye.
a)
Artificial or synthetic dyes
b)
Natural dyes
c)
Chromophores
d)
Auxochrome
47.
- Simple Benzene compounds which contain these substances are called
a)
Artificial or synthetic dyes
b)
Natural dyes
c)
Chromophores
d)
Auxochrome
e)
Chromogen
48.
- The colors are not permanent, it requires the addition of an ....
a)
Artificial or synthetic dyes
b)
Natural dyes
c)
Chromophores
d)
Auxochrome
e)
Chromogen
49.
– auxiliary radical that imparts electrolytic dissociation, alters the shade of the dye, forms salts with another compound and ultimately retains the color
a)
Artificial or synthetic dyes
b)
Natural dyes
c)
Chromophores
d)
Auxochrome
e)
Chromogen
50.
those from plants and animals. Previously applied to wool or nylon
a)
Artificial or synthetic dyes
b)
Natural dyes
c)
Chromophores
d)
Auxochrome
e)
Chromogen
51.
From the core or the heartwood of Hematoxylin campechianum (Mexican Tree)
a)
Hematoxylin
b)
Cochineal dyes
c)
Orcein
52.
Vegetable dye from certain lichens which are normally colorless.
a)
Hematoxylin
b)
Cochineal dyes
c)
Orcein
53.
Treatment with Ammonia and exposure to air produces blue to violet colors.
a)
Hematoxylin
b)
Cochineal dyes
c)
Orcein
54.
- Soluble with Alkali, for Elastic Fibers
a)
Hematoxylin
b)
Cochineal dyes
c)
Orcein
55.
- Litmus came from lichens, treated with Lime and Soda, Exposed to ammonia and air. Used as an indicator
a)
Hematoxylin
b)
Cochineal dyes
c)
Orcein
56.
In orcein, Treatment with Ammonia and exposure to air produce what color?
a)
Red to purple
b)
Blue to violet
c)
Yellow to green
d)
Blue to black
e)
I am color blind
57.
An old histologic dye from the Female Cochineal bug (Coccus Cacti)
a)
Hematoxylin
b)
Cochineal dyes
c)
Orcein
58.
Treated with Alum to produce the dye Carmine
a)
Hematoxylin
b)
Cochineal dyes
c)
Orcein
59.
Best Carmine stain
a)
Powerful Chromatin and Nuclear stain
b)
Add Picric Acid (Picrocarmine
c)
Add Aluminum Chloride
60.
Best Carmine stain for glycogen
a)
Powerful Chromatin and Nuclear stain
b)
Add Picric Acid (Picrocarmine
c)
Add Aluminum Chloride
61.
– used in Neuropathological studies
a)
Powerful Chromatin and Nuclear stain
b)
Add Picric Acid (Picrocarmine
c)
Add Aluminum Chloride
62.
For Fresh Material and Smear Preparation
a)
Powerful Chromatin and Nuclear stain
b)
Add Picric Acid (Picrocarmine
c)
Add Aluminum Chloride
63.
Aluminum chrloride
a)
For Fresh Material and Smear Preparation
b)
Neuropathological studies
c)
Best Carmine stain for glycogen
64.
picric acid
a)
For Fresh Material and Smear Preparation
b)
Neuropathological studies
c)
Best Carmine stain for glycogen
65.
Powerful Chromatin and Nuclear stain
a)
For Fresh Material and Smear Preparation
b)
Neuropathological studies
c)
Best Carmine stain for glycogen
66.
Active coloring agent is Hematin (from the oxidation of the Hematoxylin or Ripening)
a)
Hematoxylin
b)
Cochineal dyes
c)
Orcein
67.
– expose the extract to air or sunlight, takes around 3-4 months
a)
Natural ripening
b)
Artificial ripening
c)
Mordant
d)
Accentuators
68.
Ripened Hematoxylin needs ... like alum, iron, chromium, and copper salts.
a)
Natural ripening
b)
Artificial ripening
c)
Mordant
d)
Accentuators
69.
Adding oxidizing agents (Hydrogen Peroxide, Mercuric Oxide, Potassium Permanganate, Sodium Perborate or Sodium Iodate)
a)
Natural ripening
b)
Artificial ripening
c)
Mordant
d)
Accentuators
70.
Natural ripening, which involve exposing the extract to air or sunlight takes around how many months?
a)
1 to 2 months
b)
3 to 4 months
c)
5 to 6 months
d)
Forever
71.
Coloring agent is on the acidic component
a)
Acid dye
b)
Basic dye
c)
Neutral dye
72.
Basic Cell structures have affinity to Acidic Stains
a)
Acid dye
b)
Basic dye
c)
Neutral dye
73.
Examples: Acid Fuchsin, Picric Acid
a)
Acid dye
b)
Basic dye
c)
Neutral dye
74.
Coloring agent is on the basic component
a)
Acid dye
b)
Basic dye
c)
Neutral dye
75.
Acidic Structures have affinity to Basic Stains
a)
Acid dye
b)
Basic dye
c)
Neutral dye
76.
Examples: Methylene Blue (can be used as an indicator and a dye) (For Bacterial Staining)
a)
Acid dye
b)
Basic dye
c)
Neutral dye
77.
Formed by combining aqueous solutions of acidic and basic dyes
a)
Acid dye
b)
Basic dye
c)
Neutral dye
78.
Can stain Cytoplasm and Nucleus simultaneously and differentially.
a)
Acid dye
b)
Basic dye
c)
Neutral dye
79.
Insoluble to barely soluble to water
a)
Acid dye
b)
Basic dye
c)
Neutral dye
80.
Soluble in Alcohol
a)
Acid dye
b)
Basic dye
c)
Neutral dye
81.
Examples: Romanowsky Dyes in Hematology, Giemsa’s Stain. Irishman’s Stain for Leukocytes differentiation
a)
Acid dye
b)
Basic dye
c)
Neutral dye
82.
can be used as a fixative, can differentiate or stain tissue by itself. Decalcifying agent and a tissue softener
a)
Acid fuchsin
b)
Carbol fuchsin
c)
Picric acid
d)
Hydrochloric acid
83.
– for granules of endocrine cells of the alimentary tract
a)
Hematoxylin
b)
Aluminum hematoxylin
c)
Iron hematoxylin
d)
Phosphotungstic acid hematoxylin
e)
Lead hematoxylin
84.
For differential and regressive staining; Uses Acid Alcohol as differentiating agent
a)
Hematoxylin
b)
Aluminum hematoxylin
c)
Iron hematoxylin
d)
Phosphotungstic acid hematoxylin
e)
Lead hematoxylin
85.
Iron salts are used as ripening agents and oxidizing agents
a)
Hematoxylin
b)
Aluminum hematoxylin
c)
Iron hematoxylin
d)
Phosphotungstic acid hematoxylin
e)
Lead hematoxylin
86.
Mordant used is 1% Aqueous Phosphotungstic Acid
a)
Hematoxylin
b)
Aluminum hematoxylin
c)
Iron hematoxylin
d)
Phosphotungstic acid hematoxylin
e)
Lead hematoxylin
87.
Progressive Stain
a)
Hematoxylin
b)
Aluminum hematoxylin
c)
Iron hematoxylin
d)
Phosphotungstic acid hematoxylin
e)
Lead hematoxylin
88.
Nuclei, Fibrin, muscle striations, Myofibrils and Fibroglia – Blue color Collagen, Bone, Cartilage – Orange-Red, Brownish Red to Deep Brick Red color.
a)
Hematoxylin
b)
Aluminum hematoxylin
c)
Iron hematoxylin
d)
Phosphotungstic acid hematoxylin
e)
Lead hematoxylin
89.
In Phosphotungstic acid hematoxylin, collagen, bone and cartilage is
a)
Blue
b)
Orange-Red, Brownish Red to Deep Brick Red color.
90.
In Phosphotungstic acid hematoxylin, Nuclei, Fibrin, muscle striations, Myofibrils and Fibroglia is
a)
Blue
b)
Orange-Red, Brownish Red to Deep Brick Red color.
91.
Stain becomes more selective for nuclei if acid or an excess of Ferric salts are added.
a)
Hematoxylin
b)
Aluminum hematoxylin
c)
Iron hematoxylin
d)
Phosphotungstic acid hematoxylin
e)
Lead hematoxylin
92.
for routine histologic studies, needs the mordant Iron and Alum.
a)
Hematoxylin
b)
Aluminum hematoxylin
c)
Iron hematoxylin
d)
Phosphotungstic acid hematoxylin
e)
Lead hematoxylin
93.
For Progressive staining and Regressive Staining
a)
Hematoxylin
b)
Aluminum hematoxylin
c)
Iron hematoxylin
d)
Phosphotungstic acid hematoxylin
e)
Lead hematoxylin
94.
Aluminum salts give a blue lake
a)
Hematoxylin
b)
Aluminum hematoxylin
c)
Iron hematoxylin
d)
Phosphotungstic acid hematoxylin
e)
Lead hematoxylin
95.
Ehrlich's hematoxylin falls under..
a)
Hematoxylin
b)
Aluminum hematoxylin
c)
Iron hematoxylin
d)
Phosphotungstic acid hematoxylin
e)
Lead hematoxylin
96.
Cole's hematoxylin falls under..
a)
Hematoxylin
b)
Aluminum hematoxylin
c)
Iron hematoxylin
d)
Phosphotungstic acid hematoxylin
e)
Lead hematoxylin
97.
Aluminum hematoxylin
a)
Regressive stain only
b)
Progressive stain only
c)
Regressive and progressive stain
98.
Ehrlich's hematoxylin
a)
Regressive stain only
b)
Progressive stain only
c)
Regressive and progressive stain
99.
Harris hematoxylin
a)
Regressive stain only
b)
Progressive stain only
c)
Regressive and progressive stain
100.
Heidenhain's hematoxylin
a)
Regressive stain only
b)
Progressive stain only
c)
Regressive and progressive stain
101.
Mayer's hematoxylin
a)
Regressive stain only
b)
Progressive stain only
c)
Regressive and progressive stain
102.
Harris hematoxylin falls under..
a)
Hematoxylin
b)
Aluminum hematoxylin
c)
Iron hematoxylin
d)
Phosphotungstic acid hematoxylin
e)
Lead hematoxylin
103.
Mayer's hematoxylin falls under..
a)
Hematoxylin
b)
Aluminum hematoxylin
c)
Iron hematoxylin
d)
Phosphotungstic acid hematoxylin
e)
Lead hematoxylin
104.
Carazzi's hematoxylin falls under..
a)
Hematoxylin
b)
Aluminum hematoxylin
c)
Iron hematoxylin
d)
Phosphotungstic acid hematoxylin
e)
Lead hematoxylin
105.
Weigert's hematoxylin falls under..
a)
Hematoxylin
b)
Aluminum hematoxylin
c)
Iron hematoxylin
d)
Phosphotungstic acid hematoxylin
e)
Lead hematoxylin
106.
Heidenhain's hematoxylin falls under..
a)
Hematoxylin
b)
Aluminum hematoxylin
c)
Iron hematoxylin
d)
Phosphotungstic acid hematoxylin
e)
Lead hematoxylin
107.
Verhoeff hematoxylin falls under..
a)
Hematoxylin
b)
Aluminum hematoxylin
c)
Iron hematoxylin
d)
Phosphotungstic acid hematoxylin
e)
Lead hematoxylin
108.
Loyez hematoxylin falls under..
a)
Hematoxylin
b)
Aluminum hematoxylin
c)
Iron hematoxylin
d)
Phosphotungstic acid hematoxylin
e)
Lead hematoxylin
109.
Regressive Staining
a)
Ehrlich's hematoxylin
b)
Cole's hematoxylin
c)
Harris hematoxylin
d)
Mayer's hematoxylin
e)
Carazzi's Hematoxylin
110.
Cartilage, Cement lines of bones – intensely blue.
a)
Ehrlich's hematoxylin
b)
Cole's hematoxylin
c)
Harris hematoxylin
d)
Mayer's hematoxylin
e)
Carazzi's Hematoxylin
111.
Not ideal for Frozen Sections
a)
Ehrlich's hematoxylin
b)
Cole's hematoxylin
c)
Harris hematoxylin
d)
Mayer's hematoxylin
e)
Carazzi's Hematoxylin
112.
Suitable for tissues undergone acid decalcification
a)
Ehrlich's hematoxylin
b)
Cole's hematoxylin
c)
Harris hematoxylin
d)
Mayer's hematoxylin
e)
Carazzi's Hematoxylin
113.
Ripened by Sodium Iodate
a)
Ehrlich's hematoxylin
b)
Cole's hematoxylin
c)
Harris hematoxylin
d)
Mayer's hematoxylin
e)
Carazzi's Hematoxylin
114.
Used in sequence with Celestine Blue
a)
Ehrlich's hematoxylin
b)
Cole's hematoxylin
c)
Harris hematoxylin
d)
Mayer's hematoxylin
e)
Carazzi's Hematoxylin
115.
Artificially ripened with Alcoholic Iodine solution.
a)
Ehrlich's hematoxylin
b)
Cole's hematoxylin
c)
Harris hematoxylin
d)
Mayer's hematoxylin
e)
Carazzi's Hematoxylin
116.
Regressive and Progressive stain
a)
Ehrlich's hematoxylin
b)
Cole's hematoxylin
c)
Harris hematoxylin
d)
Mayer's hematoxylin
e)
Carazzi's Hematoxylin
117.
Nuclear counterstain, to show Cytoplasmic Glycogen
a)
Ehrlich's hematoxylin
b)
Cole's hematoxylin
c)
Harris hematoxylin
d)
Mayer's hematoxylin
118.
Used in Celestine Blue Hemalum nuclear staining.
a)
Ehrlich's hematoxylin
b)
Cole's hematoxylin
c)
Harris hematoxylin
d)
Mayer's hematoxylin
e)
Carazzi's Hematoxylin
119.
Ripened by Mercuric Chloride
a)
Ehrlich's hematoxylin
b)
Cole's hematoxylin
c)
Harris hematoxylin
d)
Mayer's hematoxylin
e)
Carazzi's Hematoxylin
120.
Filtration is needed and contact time is increased.
a)
Ehrlich's hematoxylin
b)
Cole's hematoxylin
c)
Harris hematoxylin
d)
Mayer's hematoxylin
e)
Carazzi's Hematoxylin
121.
Precipitation in staining solution – deterioration in the nuclear staining property
a)
Ehrlich's hematoxylin
b)
Cole's hematoxylin
c)
Harris hematoxylin
d)
Mayer's hematoxylin
e)
Carazzi's Hematoxylin
122.
Good Regressive stain; Routine Nuclear Staining
a)
Ehrlich's hematoxylin
b)
Cole's hematoxylin
c)
Harris hematoxylin
d)
Mayer's hematoxylin
e)
Carazzi's Hematoxylin
123.
ripened by Potassium Iodide, Frozen Sections
a)
Ehrlich's hematoxylin
b)
Cole's hematoxylin
c)
Harris hematoxylin
d)
Mayer's hematoxylin
e)
Carazzi's Hematoxylin
124.
frozen sections
a)
Weigert's hematoxylin
b)
Heidenhain's hematoxylin
c)
Loyez hematoxylin
d)
Verhoeff hem
125.
Elastic Fibers stained Black.
a)
Weigert's hematoxylin
b)
Heidenhain's hematoxylin
c)
Loyez hematoxylin
d)
Verhoeff hematoxylin
126.
Ferric Ammonium Sulfate is the Mordant.
a)
Weigert's hematoxylin
b)
Heidenhain's hematoxylin
c)
Loyez hematoxylin
d)
Verhoeff hematoxylin
127.
Cytological staining; Regressive Staining
a)
Weigert's hematoxylin
b)
Heidenhain's hematoxylin
c)
Loyez hematoxylin
d)
Verhoeff hematoxylin
128.
Oxidizer used is Potassium Permanganate
a)
Weigert's hematoxylin
b)
Heidenhain's hematoxylin
c)
Loyez hematoxylin
d)
Verhoeff hematoxylin
129.
Chromatins, Chromosomes, Nucleoli, Centrosomes, Mitochondria is demonstrated.
a)
Weigert's hematoxylin
b)
Heidenhain's hematoxylin
c)
Loyez hematoxylin
d)
Verhoeff hematoxylin
130.
Voluntary Muscles striations and Myelin are also well stained.
a)
Weigert's hematoxylin
b)
Heidenhain's hematoxylin
c)
Loyez hematoxylin
d)
Verhoeff hematoxylin
131.
Standard Iron Hematoxylin (Muscle fibers and Connective Tissues)
a)
Weigert's hematoxylin
b)
Heidenhain's hematoxylin
c)
Loyez hematoxylin
d)
Verhoeff hematoxylin
132.
Ferric Ammonium Chloride is the Mordant
a)
Weigert's hematoxylin
b)
Heidenhain's hematoxylin
c)
Loyez hematoxylin
d)
Verhoeff hematoxylin