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WorksheetsENV 111 (pt3)
Total questions: 34
Worksheet time: 17mins
Name
Class
Date
1.
Which one of the following is not a characteristic of bacteria?
a)
They are prokaryotic.
b)
They have peptidoglycan cell walls.
c)
They are of the same shape.
d)
They grow by binary fission.
2.
The application of iodine in Gram stain technique is to
a)
increase the affinity of primary stain for bacterial cells
b)
stain those bacterial cells that had been decolorized
c)
decolorize the stain taken up by bacterial cells
d)
form a complex with the counterstain, safranin
3.
___ is an acidic dye.
a)
Safranin
b)
Eosin
c)
Crystal violet
d)
Malachite green
4.
On applying malachite green with heat on Bacillus smear and counterstaining with safranin, the green structures observed under the microscope are
a)
cell wall
b)
capsules
c)
endospores
d)
flagella
5.
Which one of the following is FALSE about bacterial glycocalyx?
a)
It is made inside the cell and secreted to the cell surface.
b)
It is made up either polysaccharide or polypeptide, or both.
c)
Firmly attached to the cell wall, it is described as a capsule.
d)
Capsule can be determined by using Gram staining.
6.
Bacteria that have a tuft of flagella at each end of the cell are referred to as
a)
monotrichous
b)
peritrichous
c)
amphitrichous
d)
lophotrichous
7.
All of the following are examples of bioremediation EXCEPT
a)
Application of oil-degrading bacteria to an oil spill.
b)
Bacterial production of human protein such as interferon.
c)
The use of bacterial enzymes as drain cleaners.
d)
The use of insecticides to control mosquitoes.
8.
The lag phase of bacterial growth corresponds to a period of
a)
synthesis of enzymes and various molecules
b)
equilibrium of the bacterial population
c)
growth or exponential growth phase
d)
slowing down of metabolic activities
9.
Microaerophiles are microbes that grow in ___ than that in the air.
a)
nitrogen concentration higher
b)
carbon dioxide concentration higher
c)
oxygen concentration lower
d)
oxygen concentration higher
10.
Viruses are entities that contain
a)
both of the nucleic acids, DNA and RNA
b)
protein coat that surrounds the nucleic acid
c)
enzymes of their own for metabolism
d)
single type of nucleic acids, the RNA
11.
Viruses may be classified into different morphological types on the basis of their
a)
nucleic acid
b)
spike
c)
capsid
d)
tail fibre
12.
Phage lysozyme is released during ___ stage.
a)
penetration
b)
attachment
c)
assembly
d)
maturation
13.
The final stage of viral multiplication is the ___ stage.
a)
penetration
b)
biosynthesis
c)
maturation
d)
release
14.
Systemic mycoses are fungal infections ___
a)
of hair and nails
b)
beneath the skin
c)
deep within the body
d)
surface epidermal cells
15.
Yeast infection is most frequently caused by
a)
Candida albicans
b)
Rhizopus
c)
Aspergillus
d)
Penicillium
16.
Ringworm, a ___ mycosis, is caused by ___
a)
systemic, Tinea pedis
b)
cutaneous, Tinea capitis
c)
subcutaneous, Tinea unguium
d)
systemic, Tinea solium
17.
___ is the causative agent of the disease pneumonia.
a)
Chlamydophila psittaci
b)
Chlamydophila pneumoniae
c)
Rickettsia rickettsii
d)
Chlamydia trachomatis
18.
The following are the factors influencing incubation period of an infection EXCEPT the
a)
pathogen
b)
pathogenicity
c)
number of pathogen
d)
environment
19.
All of the following are diseases spread by direct contact transmission EXCEPT
a)
influenza
b)
measles
c)
tetanus
d)
syphilis
20.
Which of the following pairs is MISMATCHED?
a)
Mycobacterium tuberculosis – tuberculosis
b)
Clostridium botulinum – tetanus
c)
Plasmodium vivax – malaria
d)
Aedes aegypti – dengue
21.
Which of the following pairs is MISMATCHED?
a)
Dermatophytes – fungi that infect only the epidermis, hair and nails.
b)
Saprophytes – organisms that obtain nutrients from dead organic matter.
c)
Parasites – organisms that obtain nutrients from living host.
d)
Leucocytes – red blood cells involved in transport of O₂ and CO₂.
22.
Exotoxins attacking the lining of the gastrointestinal tract are called
a)
cardiotoxins
b)
hepatotoxins
c)
enterotoxins
d)
neurotoxins
23.
The portal of entry of Salmonella typhi, which causes ___, is through the ___ tract.
a)
typhoid fever
b)
salmonellosis
c)
candidiasis
d)
shigellosis
24.
Incineration uses the principle of
a)
filtration
b)
pasteurization
c)
dry heat sterilization
d)
autoclaving
25.
Which of the following is most likely to be bactericidal?
a)
Membrane filtration
b)
Ionizing radiation
c)
Freeze-drying
d)
Deep-freezing
26.
Which one of the following statements is FALSE about the transfer of bacteria from one culture medium to another?
a)
The transfer is done by using sterile loop.
b)
The transfer is done by using plastic pipette.
c)
The transfer is to keep the bacteria alive.
d)
The transfer is done in an aseptic manner.
27.
Given below are procedures for the spread plate method EXCEPT
a)
Bacterial suspension is introduced into a sterile Petri dish.
b)
The bacterial suspension is evenly spread over the solidified agar.
c)
The plate is then incubated when the agar surface appears dry.
d)
Bacterial suspension is introduced onto a solidified agar medium.
28.
Which one of the following statements is FALSE about turbidity used in estimation of bacterial numbers?
a)
The instrument used to measure turbidity is spectrophotometer.
b)
As turbidity increases, more light will reach the photoelectric cell.
c)
Turbidity is a result of bacterial growth on a solidified medium.
d)
Turbidity is an indirect method used in estimation of bacterial numbers.
29.
The culture media designed to increase numbers of desired microbes to detectable levels is known as
a)
selective media
b)
reducing media
c)
enrichment media
d)
culture media
30.
Which one of the following media would be used to differentiate colonies of desired microbes from others?
a)
Selective media
b)
Enrichment media
c)
Reducing media
d)
Differential media
31.
In complex media, the energy, carbon, nitrogen, and sulfur requirements are provided by
a)
yeast extracts in the media
b)
protein in the media
c)
agar added to the media
d)
sodium chloride in the media
32.
Bismuth sulfite agar is used to encourage the growth of
a)
Salmonella typhi from faeces
b)
Escherichia coli from water
c)
Streptococcus pyogenes
d)
Staphylococcus aureus
33.
The purpose of adding sodium thioglycolate to a culture medium is to
a)
increase the oxygen in the culture medium
b)
supply sodium to the culture medium
c)
deplete the oxygen in the culture medium
d)
increase the pH of the culture medium
34.
Deep-freezing is a process in which a pure culture of microbes is placed in a suspending liquid and quick-frozen at temperatures ranging from
a)
50° to 95°C
b)
-50° to +95°C
c)
-50° to -95°C
d)
-54° to -72°C
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