wayground logo

Free Printable Worksheets

NEW

Font size

S
M
L
XL
Worksheets

Ag Biotech #2

Total questions: 50

Worksheet time: 25mins

Name
Class
Date
1.

The goals of biotechnology include making plants

a)

Higher yielding

b)

Heathier

c)

Added nutrition

d)

All of the above

2.

Developing countries may benefit from biotechnology through

a)

Increased food availability

b)

Better nutrition

c)

Vaccinations and disease prevention

d)

All of the above

3.

All genetic information needed to make an entire organism is contained in

a)

Protein

b)

Cells

c)

DNA

d)

Plastids

4.

Once a gene has been transferred into an organism, the organism may now be referred to as

a)

Transformed

b)

Transgenic

c)

Both a and b

d)

Neither a nor b

5.

A genetically identical organism is referred to as a

a)

Clone

b)

Miracle of science

c)

Dogma

d)

Gamete

6.

Gene transfer in plants is commonly mediated through the bacterium

a)

Bacillus thuringiensis

b)

Escherichia coli

c)

Agrobacterium

d)

None of the above

7.

The building blocks of proteins are known as

a)

Carbohydrates

b)

Cytokinin

c)

Sugars

d)

Amino acids

8.

The bases in DNA bind in the following manner

a)

A with T, G with U

b)

A with T, G with C

c)

A with U, T with C

d)

C with G, T with U

9.

The four bases found in RNA are:

a)

Cytosine, Thymine, Adenine, Guanine

b)

Cytosine, Uracil, Adenine, Guanine

c)

Cytosine, Uracil, Thymine, Guanine

d)

Cytosine, Thymine, Uracil, Guanine

10.

What is the complementary DNA strand for the sequence GACATGGC?

a)

GACATGGC

b)

TGCGATTG

c)

CTGTACCG

d)

GUCUTAAC

11.

Rank the following in order from largest to smallest: Protein, Chromosome, Nucleotide, cell

a)

Cell, chromosome, protein, nucleotide

b)

Protein, cell, nucleotide, chromosome

c)

Chromosome, protein, cell, nucleotide

d)

Nucleotide, protein, chromosome, cell

12.

What is the name of the laboratory technique that rapidly produces millions to copies of a specific DNA sequence?

a)

Gel electrophoresis

b)

Polymerase Chain Reaction (PCR)

c)

Recombination

d)

Centrifugation

13.

What is the name of the technique that is common in laboratories that will separate DNA fragments according to their size?

a)

Gel electrophoresis

b)

Polymerase Chain Reaction (PCR)

c)

Recombination

d)

Centrifugation

14.

When separating DNA fragments according to their size, which molecules will travel the fastest?

a)

Larger

b)

Positive charged

c)

Smaller

d)

Negative charged

15.

When the Arctic Apple hit the market in the fall of 2017, it was marketed as an apple that does not ____________.

a)

Brown

b)

Have seeds

c)

Get infected with worms

d)

Have skin

16.

The three organizations that regulate biotech foods are ________________.

a)

PDQ, FCE, EPA

b)

USDA, EPA, FDA

c)

FDA, EPC, USQA

d)

EPA, USDA, FPD

17.

Potency refers to the cells ability to differentiate. Which of the following has the most specific limitation in terms of what it can differentiate into (in other words, which cell type can only differentiate into one type of cell)?

a)

Unipotent

b)

Multipotent

c)

Oligopotent

d)

Totipotent

18.

Gene therapy treatment of severe combined immunodeficiency (SCID) includes removing bone marrow from the patient. Then, a normal gene for the enzyme adenosine deaminase is introduced into the cells using a _____________. The enzyme reverse transcriptiionase will then allow the RNA to become DNA.

a)

Introvirus

b)

Exovirus

c)

Retrovirus

d)

Antiretrovirus

19.

In order for intracellular proteins to be extracted and purified, cell membranes must be broken. Some common methods for completing this are mechanical, chemical and ________________.

a)

Swirling

b)

Inverting

c)

Fermenting

d)

Sonicating

20.

Using electricity to create temporary holes in cellular membranes to allow foreign DNA into cells is called ________________.

a)

Jolting

b)

Hertzattion

c)

Electroporation

d)

Splicing

21.

____________ is an enzyme that was historically harvested from the stomach lining of cows. This was used in cheese production. Today, more than 75% of the cheese produced uses a version of that enzymes that was produced in genetically engineered bacteria.

a)

Glucosinolate

b)

Sodium dodecyl sulfate

c)

Solinane

d)

Renin

22.

Posttranscriptional gene silencing results from RNA molecules that inhibit gene __________________.

a)

Transfer

b)

Expression

c)

Replication

d)

Positioning

23.

____________ gives scientists the ability to determine function of genes, as well as any mutations that may cause a disease, disorder or cancer.

a)

Gene profiling

b)

Gene mapping

c)

Gene regulation

d)

Gene shuffling

24.

In the Flavr Savr Tomato, antisense RNA ____________ the gene polygalacturonase.

a)

Increases

b)

Silences

c)

Improves

d)

Slightly decreases

25.

Okazaki fragments are small fragments of DNA that form on the ____________ strand during DNA replication.

a)

Leading

b)

Lagging

c)

Twisted

d)

Prime

26.

The genotype of a rose with a red flower is Rr, where "R" is dominant. The "r" would be considered to be

a)

Co-dominant

b)

Recessive

c)

Co-recessive

d)

Incomplete dominance

27.

Rr and RR would have

a)

The same phenotype

b)

Different phenotypes

c)

A blend of the two phenotypes

d)

Ability to exhibit both phenotypes simultaneously

28.

Cell division, which results in the creation of two genetically identical cells, is called

a)

Meiosis

b)

Mitosis

c)

Both a and b

d)

Neither a nor b

29.

A corn plant has the genotype BbRr where BB and Bb plants are resistant to corn borers while bb plants are not AND RR and Rr plants are resistant to Roundup Herbicide while rr plants are not. A corn breeder crosses the BbRr plant with a bbrr plant and produces hundreds of offspring. They grow hundreds of plants from these seeds. What fraction of these offspring will be resistant to corn borers AND Roundup?

a)

1/4 x 1/4 = 1/16

b)

1/2 x 1/2 = 1/4

c)

1/2 x 1/2 = 1

d)

3/4 x 3/4 = 9/16

30.

Below are the five steps needed to create a new corn hybrid that would be genetically resistant to herbicide X. Please place these steps in the proper order. 1- Cross the transgenic corn plant with other corn plants that can make superior hybrids 2- Extract all genes from a strain of bacteria that is resistant to herbicide X 3- Insert the transgene into corn cells growing in tissue culture and generate a cloned plant from those transformed cells 4- Modify the herbicide X resistance gene so that the gene information can be read by the corn plant in order to make resistance proteins in all cells of the plant 5- Isolate and clone the herbicide X resistance gene from the resistant strain of bacteria

a)

2, 5, 4, 3, 1

b)

1, 2, 4, 3, 5

c)

3, 5, 4, 2, 1

d)

4, 5, 2, 3, 1

31.

The ____________ uses tungsten or gold bullets coated with DNA is a method to insert new DNA into desired plant tissues.

a)

Direct gene transfer to protoplasts

b)

Ballistic gene gun

c)

CRISPR

d)

Electroporation

32.

BT corn is resistant to

a)

Herbicides

b)

Drought

c)

Soybean Cyst Nematodes

d)

Corn Borer

33.

Of the following, which is most likely to increase a plant's productivity?

a)

Decreasing the rate of photosynthesis

b)

Increasing the rate of photosynthesis

c)

Maintaining the rate of photosynthesis

d)

Eliminating the need for photosynthesis

34.

Auxin, Gibbrellins, and Cytokinins are all

a)

Plant growth promoters

b)

Plant growth inhibitors

c)

Maintain current plant growth

35.

Ethylene and Abscisic Acid are examples of

a)

Plant growth promoters

b)

Plant growth inhibitors

c)

Maintain current plant growth

36.

The genotype RR would be considered

a)

Homozygous

b)

Heterozygous

c)

Hemizygous

d)

Retrozygous

37.

Artificial selection occurs __________ than natural selection.

a)

Faster

b)

Slower

c)

At the same rate

d)

In tandem

38.

According to __________, the first trait given from the parent to the offspring will not influence the remaining traits to be given.

a)

Law of Segregation

b)

Law of Independent Assortment

c)

Law of Dominance

d)

Law of Separation

39.

__________ is a technology used to directly edit or alter DNA in a very specific manner.

a)

TIGR

b)

FIRMR

c)

CRISPR

d)

WELTR

40.

In bacteria, the plasmid DNA is the circular DNA that is only functional in the organism when it is facing certain conditions such as __________.

a)

Stress due to disease or insects

b)

Stress due to presence of antibiotic

c)

Stress due to environmental conditions

d)

All of the above

41.

Golden Rice was created to contain elevated amounts of __________ to combat nutrient deficiencies that lead to blindness, limited growth, and weakened immune systems common in third world countries.

a)

Vitamin D

b)

Vitamin B12

c)

Vitamin A

d)

Vitamin C

42.

Xenotransplantation is the process of replacing an animal organ with an organ from a human.

a)

True

b)

False

43.

Which of the following is found naturally occurring in living things?

a)

Genes that form new versions or alleles by random mistakes in replication

b)

A genetically modified organism that contains a gene that was transferred from another species

c)

A chromosome that can recombine with other chromosomes

d)

All of the above

44.

Which is NOT true of proteins?

a)

Proteins have very little use in animals

b)

Proteins are the biomolecule that actually performs activities in cells such as catalyzing biochemical reactions, controlling gene expressions, altering cell shapes

c)

The structure of proteins is ultimately determined by the information stored in DNA

d)

All of the above are true statements about proteins

45.

Using the codon chart below, determine the amino acid encoded by the bases CAU.

a)

Threonine

b)

Histidine

c)

Leucine

d)

Tyrosine

46.

There are two genes that a genetic engineer could work with, Gene A and Gene B. Gene A is from algae, is turned on in salt water, and encodes an enzyme that breaks down oil. Gene B is from soil bacteria, is turned on in soil, and encodes an enzyme that breaks down plant material. Which transgene could be used to create a genetically engineered soil bacteria that can clean up oil spills?

a)

Use gene A

b)

Use the promoter from gene B and the coding region from gene A

c)

Use gene B

d)

Use the promoter from gene A and the coding region from gene B

47.

Which plants typically respond best to agrobacterium mediated transfer?

a)

Monocots

b)

Dicots

c)

Tricots

d)

All of the above

48.

In a laboratory setting, it is common to use the same protocol numerous times when conducting an experiment. Therefore, it is common practice to refer to a specific SOP rather than writing out each step in the protocol every time it is used. What does SOP stand for?

a)

Standard operating platform

b)

Standard operating procedure

c)

Standard official protocol

d)

Standard obligatory procedure

49.

The Central Dogma of molecular biology is DNA→RNA→Protein. __________ is what occurs when going from DNA to RNA.

a)

Replication

b)

Transcription

c)

Translation

d)

Reverse transcription

50.

The Central Dogma of molecular biology is DNA→RNA→Protein. __________ is what occurs when going from RNA to Protein.

a)

Replication

b)

Transcription

c)

Translation

d)

Reverse transcription