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WorksheetsAg Biotech #2
Total questions: 50
Worksheet time: 25mins
The goals of biotechnology include making plants
Higher yielding
Heathier
Added nutrition
All of the above
Developing countries may benefit from biotechnology through
Increased food availability
Better nutrition
Vaccinations and disease prevention
All of the above
All genetic information needed to make an entire organism is contained in
Protein
Cells
DNA
Plastids
Once a gene has been transferred into an organism, the organism may now be referred to as
Transformed
Transgenic
Both a and b
Neither a nor b
A genetically identical organism is referred to as a
Clone
Miracle of science
Dogma
Gamete
Gene transfer in plants is commonly mediated through the bacterium
Bacillus thuringiensis
Escherichia coli
Agrobacterium
None of the above
The building blocks of proteins are known as
Carbohydrates
Cytokinin
Sugars
Amino acids
The bases in DNA bind in the following manner
A with T, G with U
A with T, G with C
A with U, T with C
C with G, T with U
The four bases found in RNA are:
Cytosine, Thymine, Adenine, Guanine
Cytosine, Uracil, Adenine, Guanine
Cytosine, Uracil, Thymine, Guanine
Cytosine, Thymine, Uracil, Guanine
What is the complementary DNA strand for the sequence GACATGGC?
GACATGGC
TGCGATTG
CTGTACCG
GUCUTAAC
Rank the following in order from largest to smallest: Protein, Chromosome, Nucleotide, cell
Cell, chromosome, protein, nucleotide
Protein, cell, nucleotide, chromosome
Chromosome, protein, cell, nucleotide
Nucleotide, protein, chromosome, cell
What is the name of the laboratory technique that rapidly produces millions to copies of a specific DNA sequence?
Gel electrophoresis
Polymerase Chain Reaction (PCR)
Recombination
Centrifugation
What is the name of the technique that is common in laboratories that will separate DNA fragments according to their size?
Gel electrophoresis
Polymerase Chain Reaction (PCR)
Recombination
Centrifugation
When separating DNA fragments according to their size, which molecules will travel the fastest?
Larger
Positive charged
Smaller
Negative charged
When the Arctic Apple hit the market in the fall of 2017, it was marketed as an apple that does not ____________.
Brown
Have seeds
Get infected with worms
Have skin
The three organizations that regulate biotech foods are ________________.
PDQ, FCE, EPA
USDA, EPA, FDA
FDA, EPC, USQA
EPA, USDA, FPD
Potency refers to the cells ability to differentiate. Which of the following has the most specific limitation in terms of what it can differentiate into (in other words, which cell type can only differentiate into one type of cell)?
Unipotent
Multipotent
Oligopotent
Totipotent
Gene therapy treatment of severe combined immunodeficiency (SCID) includes removing bone marrow from the patient. Then, a normal gene for the enzyme adenosine deaminase is introduced into the cells using a _____________. The enzyme reverse transcriptiionase will then allow the RNA to become DNA.
Introvirus
Exovirus
Retrovirus
Antiretrovirus
In order for intracellular proteins to be extracted and purified, cell membranes must be broken. Some common methods for completing this are mechanical, chemical and ________________.
Swirling
Inverting
Fermenting
Sonicating
Using electricity to create temporary holes in cellular membranes to allow foreign DNA into cells is called ________________.
Jolting
Hertzattion
Electroporation
Splicing
____________ is an enzyme that was historically harvested from the stomach lining of cows. This was used in cheese production. Today, more than 75% of the cheese produced uses a version of that enzymes that was produced in genetically engineered bacteria.
Glucosinolate
Sodium dodecyl sulfate
Solinane
Renin
Posttranscriptional gene silencing results from RNA molecules that inhibit gene __________________.
Transfer
Expression
Replication
Positioning
____________ gives scientists the ability to determine function of genes, as well as any mutations that may cause a disease, disorder or cancer.
Gene profiling
Gene mapping
Gene regulation
Gene shuffling
In the Flavr Savr Tomato, antisense RNA ____________ the gene polygalacturonase.
Increases
Silences
Improves
Slightly decreases
Okazaki fragments are small fragments of DNA that form on the ____________ strand during DNA replication.
Leading
Lagging
Twisted
Prime
The genotype of a rose with a red flower is Rr, where "R" is dominant. The "r" would be considered to be
Co-dominant
Recessive
Co-recessive
Incomplete dominance
Rr and RR would have
The same phenotype
Different phenotypes
A blend of the two phenotypes
Ability to exhibit both phenotypes simultaneously
Cell division, which results in the creation of two genetically identical cells, is called
Meiosis
Mitosis
Both a and b
Neither a nor b
A corn plant has the genotype BbRr where BB and Bb plants are resistant to corn borers while bb plants are not AND RR and Rr plants are resistant to Roundup Herbicide while rr plants are not. A corn breeder crosses the BbRr plant with a bbrr plant and produces hundreds of offspring. They grow hundreds of plants from these seeds. What fraction of these offspring will be resistant to corn borers AND Roundup?
1/4 x 1/4 = 1/16
1/2 x 1/2 = 1/4
1/2 x 1/2 = 1
3/4 x 3/4 = 9/16
Below are the five steps needed to create a new corn hybrid that would be genetically resistant to herbicide X. Please place these steps in the proper order. 1- Cross the transgenic corn plant with other corn plants that can make superior hybrids 2- Extract all genes from a strain of bacteria that is resistant to herbicide X 3- Insert the transgene into corn cells growing in tissue culture and generate a cloned plant from those transformed cells 4- Modify the herbicide X resistance gene so that the gene information can be read by the corn plant in order to make resistance proteins in all cells of the plant 5- Isolate and clone the herbicide X resistance gene from the resistant strain of bacteria
2, 5, 4, 3, 1
1, 2, 4, 3, 5
3, 5, 4, 2, 1
4, 5, 2, 3, 1
The ____________ uses tungsten or gold bullets coated with DNA is a method to insert new DNA into desired plant tissues.
Direct gene transfer to protoplasts
Ballistic gene gun
CRISPR
Electroporation
BT corn is resistant to
Herbicides
Drought
Soybean Cyst Nematodes
Corn Borer
Of the following, which is most likely to increase a plant's productivity?
Decreasing the rate of photosynthesis
Increasing the rate of photosynthesis
Maintaining the rate of photosynthesis
Eliminating the need for photosynthesis
Auxin, Gibbrellins, and Cytokinins are all
Plant growth promoters
Plant growth inhibitors
Maintain current plant growth
Ethylene and Abscisic Acid are examples of
Plant growth promoters
Plant growth inhibitors
Maintain current plant growth
The genotype RR would be considered
Homozygous
Heterozygous
Hemizygous
Retrozygous
Artificial selection occurs __________ than natural selection.
Faster
Slower
At the same rate
In tandem
According to __________, the first trait given from the parent to the offspring will not influence the remaining traits to be given.
Law of Segregation
Law of Independent Assortment
Law of Dominance
Law of Separation
__________ is a technology used to directly edit or alter DNA in a very specific manner.
TIGR
FIRMR
CRISPR
WELTR
In bacteria, the plasmid DNA is the circular DNA that is only functional in the organism when it is facing certain conditions such as __________.
Stress due to disease or insects
Stress due to presence of antibiotic
Stress due to environmental conditions
All of the above
Golden Rice was created to contain elevated amounts of __________ to combat nutrient deficiencies that lead to blindness, limited growth, and weakened immune systems common in third world countries.
Vitamin D
Vitamin B12
Vitamin A
Vitamin C
Xenotransplantation is the process of replacing an animal organ with an organ from a human.
True
False
Which of the following is found naturally occurring in living things?
Genes that form new versions or alleles by random mistakes in replication
A genetically modified organism that contains a gene that was transferred from another species
A chromosome that can recombine with other chromosomes
All of the above
Which is NOT true of proteins?
Proteins have very little use in animals
Proteins are the biomolecule that actually performs activities in cells such as catalyzing biochemical reactions, controlling gene expressions, altering cell shapes
The structure of proteins is ultimately determined by the information stored in DNA
All of the above are true statements about proteins
Using the codon chart below, determine the amino acid encoded by the bases CAU.
Threonine
Histidine
Leucine
Tyrosine
There are two genes that a genetic engineer could work with, Gene A and Gene B. Gene A is from algae, is turned on in salt water, and encodes an enzyme that breaks down oil. Gene B is from soil bacteria, is turned on in soil, and encodes an enzyme that breaks down plant material. Which transgene could be used to create a genetically engineered soil bacteria that can clean up oil spills?
Use gene A
Use the promoter from gene B and the coding region from gene A
Use gene B
Use the promoter from gene A and the coding region from gene B
Which plants typically respond best to agrobacterium mediated transfer?
Monocots
Dicots
Tricots
All of the above
In a laboratory setting, it is common to use the same protocol numerous times when conducting an experiment. Therefore, it is common practice to refer to a specific SOP rather than writing out each step in the protocol every time it is used. What does SOP stand for?
Standard operating platform
Standard operating procedure
Standard official protocol
Standard obligatory procedure
The Central Dogma of molecular biology is DNA→RNA→Protein. __________ is what occurs when going from DNA to RNA.
Replication
Transcription
Translation
Reverse transcription
The Central Dogma of molecular biology is DNA→RNA→Protein. __________ is what occurs when going from RNA to Protein.
Replication
Transcription
Translation
Reverse transcription
