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Worksheets2026 Biology Midterm Review
Total questions: 209
Worksheet time: 5hrs 8mins
Which list correctly orders the levels of biological organization from smallest to largest?
Atoms, organelles, cells, tissues, organ, organ system, organism
Organelles, atoms, tissues, cells, organ system, organism
Atoms, cells, organelles, tissues, organ system, organism
Cells, atoms, organelles, tissues, organs, organisms
A scientist wants to test how light intensity affects plant growth. Which choice correctly identifies the variables?
Independent: soil type; Dependent: light intensity
Independent: watering schedule; Dependent: fertilizer brand
Independent: light intensity; Dependent: plant growth
Independent: plant growth; Dependent: light intensity
Which feature distinguishes prokaryotic cells from eukaryotic cells?
Eukaryotes are less complex and evolved first
Prokaryotes lack membrane-bound organelles and a nucleus
Prokaryotes have chloroplasts and large central vacuoles
Eukaryotes have cell walls and free-floating DNA
Which pairing of macromolecule with its main function is correct?
Lipids: long-term energy storage and insulation
Proteins: store genetic information in the nucleus
Carbohydrates: long-term energy storage in membranes
Nucleic acids: catalyze reactions and form hormones
In the osmosis diagram showing a semipermeable membrane, water molecules move from the side with lower solute concentration toward the side with higher solute concentration. What is the best explanation for this movement?
Water moves to equalize solute concentrations across the membrane
Water moves randomly and equally in both directions always
Water follows its concentration gradient across any membrane
Solutes push water molecules through protein pumps using ATP
A cell is placed in a hypertonic solution as depicted in the tonicity diagram. What change will most likely occur to the cell?
Cell will gain solutes and expand
Cell will have no net water movement
Cell will shrink due to water loss
Cell will swell and possibly burst
In the facilitated diffusion diagram, particles move through a channel protein from high concentration to low concentration. Which statement distinguishes this process from active transport?
It requires ATP to move molecules
It does not require cellular energy
It uses carrier proteins and ATP
It moves against the concentration gradient
Exocytosis is illustrated by vesicles fusing with the plasma membrane and releasing contents outside the cell. Which step most directly uses the phospholipid bilayer’s properties?
Vesicle formation from the Golgi apparatus
Fusion of vesicle membrane with cell membrane
Binding of cargo to receptor proteins
Transport of cargo along microtubules
In the active transport diagram, a membrane pump moves ions from low to high concentration and shows ATP converting to ADP + Pi. What is the immediate role of ATP here?
Maintains membrane fluidity during pumping
Phosphorylates the pump to drive conformational change
Provides energy to open a channel for diffusion
Binds solute to increase its affinity for the pump
Which balanced equation represents photosynthesis in plants?
Glucose → ethanol + carbon dioxide + ATP
Oxygen + glucose → carbon dioxide + water + ATP
Carbon dioxide + water → oxygen + glucose
Glucose + oxygen → carbon dioxide + water + energy
Cellular respiration primarily occurs in which organelle of eukaryotic cells?
Golgi apparatus
Chloroplast
Ribosome
Mitochondria
A runner sprints and then feels muscle burn due to lactic acid. Which scenario best explains the energy pathway and oxygen conditions?
Aerobic respiration with limited oxygen, ethanol fermentation
Anaerobic respiration with no oxygen, lactic acid fermentation
Anaerobic respiration with oxygen present, oxidative phosphorylation
Aerobic respiration with abundant oxygen, high ATP yield
Compare ATP yields: Which statement is most accurate for typical cells?
Aerobic respiration produces 2 ATP and no byproducts
Fermentation produces more ATP than aerobic respiration
Anaerobic respiration produces about 36 ATP per glucose
Aerobic respiration produces about 36 ATP per glucose
Using the normal male karyotype diagram, which pair represents the sex chromosomes for a biological male?
X and Y chromosomes
Two X chromosomes
Two Y chromosomes
W and Z chromosomes
Look at the trisomy 21 diagram. What specific abnormality is indicated at chromosome pair 21?
An extra chromosome present
A missing chromosome present
A translocation to X present
A duplicated arm missing
Which process produces haploid gametes that can lead to karyotypes with trisomies if nondisjunction occurs?
Meiosis in germ cells
Mitosis in somatic cells
Binary fission in bacteria
Budding in yeast cells
Based on the diagrams, which statement correctly distinguishes diploid and haploid cells?
Haploid cells have pairs of homologs
Diploid cells have pairs of homologs
Haploid cells equal forty-six chromosomes
Diploid cells have single chromosomes
Which enzyme lays down RNA primers to start DNA synthesis during replication?
Topoisomerase prevents helix supercoils
Helicase initiates strand separation
Ligase seals sugar-phosphate gaps
Primase creates short RNA primers
DNA replication is described as semi-conservative because:
New strands randomly join old fragments
Original strands are fully degraded
Each new molecule has one original strand
Each new molecule has two new strands
Where does transcription occur and what is its product?
Golgi, produces amino acid chains
Ribosome, produces transfer RNA
Nucleus, produces messenger RNA
Cytoplasm, produces polypeptides
Given the DNA strand ATGCATGA, what is the complementary mRNA produced during transcription?
AUGCAUGA
UACGTACT
UACGUACU
TACGTATC
If an mRNA codon is AUG, which anticodon on tRNA pairs with it at the ribosome?
TAC
AUG
CAU
UAC
Which point mutation can introduce an early stop codon into a coding sequence?
Substitution maintaining amino acid
Silent mutation in third base
Missense changing one amino acid
Nonsense creating a termination codon
A BB black cat is crossed with a Bb gray cat. What are the expected genotypes and phenotypes of the kittens?
25% BB black, 25% Bb gray, 50% bb white
50% Bb gray, 50% bb white
25% BB black, 75% Bb gray
50% BB black, 50% Bb gray
In incomplete dominance, which statement best describes the heterozygote’s phenotype?
A random mix of parental traits
A blend of the two homozygotes
Identical to the dominant homozygote
More extreme than either homozygote
A red fish (RR) crossed with a blue fish (BB) produces RB offspring. What phenotypes do RB fish show under codominance?
Red and blue spotted
All red only
Pink blended color
All blue only
Cross TtRR × ttRr. What fraction of offspring will be ttRR?
8/16 of offspring
1/16 of offspring
0/16 of offspring
4/16 of offspring
During which process does crossing over occur, and what is its main benefit?
Meiosis; increases genetic variation
Fertilization; ensures zygote formation
Mitosis; repairs DNA damage
Replication; prevents mutations
In a pedigree where affected sons are common and daughters are mostly unaffected, which inheritance pattern is most likely?
X‑linked recessive
Autosomal dominant
Autosomal codominant
Mitochondrial inheritance
A chromosome image shows a segment removed from one chromosome with no replacement. Which structural mutation is identified?
Translocation mutation
Duplication mutation
Insertion mutation
Deletion mutation
What is the function of the cell membrane?
To control what comes in and out of the cell
To break down food inside the cell
to serve as a barrier
to eat the cells food
Enzymes are an example of
Lipids
Carbohydrate
Protein
Nucleic Acid
What happens when enzymes are heated to a high temperature?
The enzymes die.
The shape of the enzyme are changed and it denatures
The enzymes' amino acid sequence changes.
The enzymes remain the same
What is the optimum temperature for this enzyme?
20 degrees Celsius
45 degrees Celsius
52 degrees Celsius
64 degrees Celsius
What can be inferred using the graph?
Cells can function equally as well at all pH values.
Cells do not function well at pH values that are too high or too low.
Cells function best at high pH values.
Cells function best at low pH values.
What is activation energy?
the energy from the sun
the energy produced by an enzyme
the energy needed to get a reaction going
none of these
Which macromolecule is used as a primary energy source? (This is why our victim who ate Olive Garden would be prepared for the gym!)
carbohydrates
lipids
proteins
nucleic acids
Some proteins are catalysts in chemical reactions, meaning they speed them up. What are these proteins called?
monomers
enzymes
quickens
neutrons
This macromolecule provides the genetic code to pass on to offspring and the template to make proteins.
carbs
lipids
proteins
nucleic acids
Amino acids are the monomer of nucleic acids.
True
False
Monosaccharides are the monomers of carbohydrates.
True
False
Fatty acids are the monomer of carbohydrates.
True
False
What is the role of lipids in living organisms?
to provide energy
long-term energy storage
tissue growth
to store and transmit genetic information
What is the role of proteins in living organisms?
to provide energy
long-term energy storage
tissue growth
to store and transmit genetic information
What type of cells have a nucelar membrane?
Prokaryotic cells
Eukaryotic cells
Which statement accurately compares eukaryotic cells and prokaryotic cells?
Eukaryotic cells are more complex than prokaryotic cells.
Eukaryotic cells replicate faster than prokaryotic cells.
Only prokaryotic cells have membrane-boudn organelles.
Only prokaryotic cells lack outer membranes and cell walls.
Identify the type of cell:
Eukaryotic cell: Animal
Eukaryotic cell: Plant
Prokaryotic cell: Bacteria
Virus
A oranisms is multicellular, has a nucelar membrane, and membrane-bound organelles, what type of cells does this organism have?
Prokaryotic cells
Eukaryotic cells
Which of these correctly identifies the two cells described in the table?
Cell 1 is eukaryotic, and Cell 2 is prokaryotic.
Cell 1 is prokaryotic and Cell 2 is eukaryotic.
Both cells 1 and 2 are prokaytoic.
Both cells 1 and 2 are eukaryotic.
Which of the following organisms is made up of prokaryotic cells?
Plants
Bacteria
Animals
Fungi
Eukaryotic cells have membrane bound organelles.
True
False
Which of the following organisms is made up of eukaryotic cells?
Plants
Bacteria
Archaea
None of the above
Which type of cell has a cell membrane?
Prokaryotic cells
Eukaryotic cells
Both prokaryotic and eukaryotic cells
Which type of cell has a cytoplasm?
Prokaryotic cells
Eukaryotic cells
Both prokaryotic and eukaryotic cells
The DNA of these cells is circular and not found within a nucleus.
Prokaryotic cells
Eukaryotic cells
Both Prokaryotic and Eukaryotic cells
What is the correct hierarchy of cells?
tissues-cells-organ systems-organs-organisms
cell-organisms-tissues-organs-organ systems
cells-tissues-organs-organ systems-organisms
organs-tissues-cells-organ systems-organisms
Stores food and water like a refrigerator for the cell
Chloroplast
Vacuole
Lysosome
Cytoplasm
This holds water, food, or waste.
lysosome
vacuole
cell wall
cell membrane
This is responsible for removing waste and dead parts from the cell.
mitochondria
lysosome
vacuole
cell wall
Process by which particles tend to move from an area where they are more concentrated to an area where they are less concentrated
Diffusion
Endocytosis
Exocytosis
Active Transport
Process of diffusion in which molecules pass across the membrane through cell membrane channels
Osmosis
Facilitated Diffusion
Active Transport
Endocytosis
Diffusion of water through a selectively permeable membrane
Osmosis
Active Transport
Exocytosis
Endocytosis
When the concentration of two solutions is the same
Hypertonic
Isotonic
Hypertonic
Osmotic pressure
When comparing two solutions, the solution with the greater concentration of solutes
Hypertonic
Isotonic
Hypotonic
Osmotic Pressure
When comparing two solutions, the solution with the lesser concentration of solutes
Hypertonic
Isotonic
Hypotonic
Osmotic Pressure
In passive transport, substances move across the cell membrane ________ the concentration gradient
AGAINST
ALONG
DOWN
BETWEEN
Two examples of passive transport are
Endocytosis and Exocytosis
Diffusion and Osmosis
Endocytosis and Diffusion
Facilitated Diffusion and Pinocytosis
The concentration of red molecules outside the cell is higher than the concentration inside the cell. The red molecules cannot penetrate the cell. The cell is inside a ______.
hypotonic solution
hypertonic solution
Which Type of solution is the middle cell placed in?
Hypertonic
Hypotonic
Isotonic
Robotonic
If the dots represent sugar what direction will the water flow?
Into the cell
Out of the cell
Both equally (equilibrium)
What happens to a cell in a hypotonic solution?
it shrinks
it swells and gets bigger
it stays the same
The products of cellular respiration
CO2
CO2, H2O
CO2, H2O, glucose
CO2, H2O, ATP
The reactants of photosynthesis are
sugar and water
sugar and oxygen
carbon dioxide and water
carbon dioxide and oxygen
What are the products of photosynthesis?
carbon dioxide and water
carbon dioxide and oxygen
glucose and oxygen
glucose and water
The diagram shows homologous chromosomes during prophase I of meiosis. Which of the following correctly describes the process being illustrated?
Segregation of sister chromatids
Mutation in which the DNA content of the gene is altered
Crossing over in which alleles are exchanged
Condensation and segregation of alleles
An organized picture of an individual's chromosomes used to diagnose extra or missing chromosomes
amniocentesis
urine analysis
blood typing
karyotype
One benefit of asexual reproduction is that it produces genetic diversity
true
false
What is the final result of meiosis?
4 genetically different haploid cells
4 genetically identical haploid cells
4 genetically different diploid cells
4 genetically identical diploid cells
Which type of cells are used for mitosis?
Somatic Cells
Germline Cells
Sperm Cells
Gametes
What is the main purpose of mitosis?
Production of gametes
Growth and repair of somatic cells
Introduction of genetic variation
Reduction of chromosome number
What is the significance of crossing over in meiosis?
It increases genetic variation
It reduces genetic variation
It ensures identical daughter cells
It prevents chromosome separation
What is the result of meiosis?
Two identical daughter cells
Four identical daughter cells
Two genetically varied daughter cells
Four genetically varied daughter cells
What is the result of mitosis?
Two identical daughter cells
Four identical daughter cells
Two genetically varied daughter cells
Four genetically varied daughter cells
What is the main purpose of meiosis?
Production of somatic cells
Growth and repair of cells
Introduction of genetic variation
Reduction of chromosome number
In the absence of oxygen, which process comes after glycolysis
fermentation
krebs cycle
electron transport chain
Which process is used to produce beer and wine?
glycolysis
the Krebs Cycle
lactic acid fermentation
alcoholic fermentation
Breathing heavily after running a race is your body's way of
making more citric acid
recharging the electron transport chain
repaying an oxygen debt
restarting glycolysis
Fermentation would be considered a (n) _______ process.
Aerobic
Anaerobic
The rungs in the ladder structure of DNA are...
a pair of amino acids
deoxyribose and phosphate
the phosphates
two nitrogen bases
The nitrogenous bases in DNA are...
deoxyribose, guanine, cytosine, thymine
adenine, guanine, cytosine, uracil
adenine, guanine, cytosine, thymine
amine, guanine, cytosine, thymine
Which is the correct representation of a nucleotide in DNA?
deoxyribose sugar, phosphate group, uracil
deoxyribose sugar, phosphate group, thymine
ribose sugar, phosphate group, thymine
ribose sugar, phosphate group, uracil
Which type of bond (between the bases) can be easily broken, allowing the two sides of DNA to move apart for use in the cell?
hydrogen bonds
covalent bonds
peptide bonds
nitrogen bonds
AAG CTT TGC
TCG ATA CGA
Proteins are chains of amino acids bonded together. Which is the correct sequence for making proteins?
Information is DNA is transferred into RNA and then made into protein
Information in DNA is formed into protein directly.
Information in RNA mutates into DNA and then is made into protein
Information in RNA uses thermal energy to make protein
A DNA segment is changed from AATTAG to AAATAG. This is a(n)
frame shift mutation
substitution
insertion
deletion
What process is shown in the picture?
commensalism
deletion
passive transport
DNA replication
5'-ATGCCAT-3'
During Transcription, DNA is converted into ____
mRNA
tRNA
rRNA
a Protein
During Translation, mRNA is converted into a _______
DNA
Protein
tRNA
rRNA
During transcription, DNA is converted in mRNA inside the _______.
Cell Membrane
Ribosome
Nucleus
Cytoplasm
During Translation, mRNA finds a ________ to make a protein.
Nucleus
Ribosome
Amino Acid
Mitochondria
Match the DNA codon ATC to the correct mRNA codon.
UAG
AUC
TAG
UUG
Match the mRNA codon UUA to the correct tRNA anti-codon
AAT
AAU
AUA
ATA
Use your codon chart to match the mRNA CUU to the correct amino acid
VAL
TYR
LEU
PHE
Use your codon chart to match the mRNA ACU to the correct amino acid.
Thr
Ala
Val
Gly
Use your codon chart to find the amino acid that codes for AGU
Tyr
Gly
Arg
Ser
Use your codon chart and find the amino acid that codes for the mRNA codon UAA
Phe
Tyr
START
STOP
T-G-A-C-C-A
T-G-A-C-C-A-A
AGA-TTC-ATA-GCG
Mutant-
AGA-TTC-AAT-AGC-G
The mutated DNA segment is TTACGCAAC. This is an example of ___ mutation.
A nucleotide base is inserted or deleted shifting the entire DNA sequence. Entire protein will be changed.
Substitution Mutation
Silent Mutation
Frameshift Mutation
Translocation
Why are insertion and deletion mutations so harmful?
They change all of the codons from the mutation on down the line, which changes the amino acid sequence
They insert things that an organism doesn't need.
They often delete things that organisms need.
Insertion and deletions are not any more harmful than substitution mutations.
Part of a chromosome
is deleted
Deletion Mutation-Gene
Translocation
Deletion Mutation -Chromosomal
Crossing Over
Materials that can cause mutations
Reversals
Alkaline
Mutagens
Mutaters
A mutation can be...
harmful
helpful
neutral
harmful or helpful, but never neutral
harmful, helpful, AND neutral
The following pedigree chart shows _______ females and ______ males that DO NOT have the trait.
7, 3
4, 4
3, 2
3, 3
57, 93.5
How many males are carriers?
2
3
4
5
Is this pedigree dominant, recessive, or sex-linked recessive?
Dominant
Recessive
Sex-Linked Recessive
Is this pedigree dominant, recessive, or sex-linked recessive?
Dominant
Recessive
Sex-Linked Recessive
T - tall and t = short
In the punnett square below, what belongs in the missing square
tt
Tt
Bb
TT
The blending of two traits is known as ---
incomplete dominance
codominance
A condition in which both alleles for a gene are expressed so that both traits are visible is known as ---
Codominance
Incomplete dominance
What type of inheritance pattern is shown here?
Codominance
Incomplete dominance
A long-haired female rabbit and a short-haired male rabbit produced this offspring with a medium-length coat:
Incomplete dominance
Codominance
Complete dominance
In certain rats, black fur (B) is dominant over white fur (b). If two rats, both heterozygous (Bb) for fur color, are mated, their offspring would be expected to have
three different genotypes and three different colors.
three different genotypes and two different colors.
two different genotypes and three different colors.
four different genotypes and two different colors.
The physical appearance of a trait is known as the-
genotype
phenotype
Homozygous
intelligence
Based off this punnett square, what fraction of the offspring will have wrinkled, yellow seeds?
9/16
3/16
1/16
16/16
What is the genotype for a pea plant homozygous for round seeds (R), and heterozygous for yellow seeds?
RrYy
RRyy
RRYy
rryy
A white mouse with red eyes is crossed with a black mouse with brown eyes. The white mouse is recessive for both of its traits. The black mouse is dominant for both of its traits.
This cross would be
BBEE x bbee
BBEe x BbEe
BbEE x BbEe
BBee x BBEE
In a dihybrid cross, if one parent is heterozygous for both traits (RrYy) and the other parent is homozygous recessive for both traits (rryy), what will be the genotype of the offspring?
The genotype of the offspring will be RrYy, Rryy, rrYy, or rryy.
RRyy
RrYY
rrYY
What is the phenotypic ratio of a dihybrid cross between two individuals who are heterozygous for both traits?
9:3:3:1
1:1:1:1
4:4:2:2
6:2:2:1
Colorblindness and hemophilia alleles are found on
autosomal chromosomes
sex chromosomes
Which sex chromosomes do females have?
XX
XY
Which sex chromosomes do males have?
XX
XY
X-Linked traits are more common in
males
females
Hemophilia is an x-linked recessive trait. Which genotype represents a carrier female?
XHXH
XHXh
XhXh
XHT
XhY
Hemophilia (h) is an x-linked recessive trait. What are the chances that a daughter would be a carrier?
0%
25%
50%
100%
Color-blindness is an x-linked recessive trait. Which genotype represents a colorblind female?
XBXB
XBXb
XbXb
XBY
XbY
Hemophilia (h) is an x-linked recessive trait. What is the genotype of Charles and Marie's child?
XHXH
XHXh
XhXh
XHY
XhY
On a pedigree, this symbol represents a ----
not affected male.
not affected female.
affected male.
affected female.
How are individuals III-2 and II-4 related?
Brother and sister
Dad and daughter
Uncle and niece
Grandfather and granddaughter
If this pedigree shows a recessive disorder, what MUST the genotype of individual 4 be?
HH
Hh
hh
