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WorksheetsBiotechnology Practice Exam — Applications of Biotechnology
Total questions: 68
Worksheet time: 34mins
Select the best answer for each scenario. A student is preparing agarose gel for DNA separation. The gel solidifies before being poured. What should be done next?
Add buffer
Reheat until liquid
Add DNA
Increase voltage
Select the best answer for each scenario. No colonies grow on a transformation plate. What should be checked first?
Incubation time
Antibiotic activity and plasmid addition
Gel concentration
Plate labeling
Select the best answer for each scenario. A culture spills on the bench. What is the correct response?
Dry wipe
Leave it
Disinfect and follow biohazard cleanup
Rinse with water
Select the best answer for each scenario. Which tool transfers 20 µL accurately?
Beaker
Micropipette
Dropper
Cylinder
Select the best answer for each scenario. PCR fails to amplify DNA. Most likely cause?
Missing primers
Too much agarose
Gel run too long
Extra buffer
Select the best answer for each scenario. Which control confirms bacteria can grow normally?
Plate without antibiotic
Plate with antibiotic
Plasmid plate
Gel lane
Select the best answer for each scenario. A lab report lacks interpretation of results. What is missing?
Title
Procedure
Discussion
Data
Select the best answer for each scenario. A company wants to reduce chemical waste. Which biotech field applies?
Medical
Industrial
Agricultural
Therapeutic
Select the best answer for each scenario. A pipette tip touches the bench before dispensing. Biggest risk?
Evaporation
Contamination
Tip damage
Volume change
Select the best answer for each scenario. Which practice improves data reliability?
Changing variables
Consistent measurements
Skipping controls
Recording final data only
Select the best answer for each scenario. DNA bands smear on a gel. Likely cause?
Overloading sample
Fresh buffer
Correct voltage
New tray
Select the best answer for each scenario. Which document lists chemical hazards and handling?
Equipment log
SDS
Lab manual
Checklist
Select the best answer for each scenario. Wrong incubator temperature may:
Improve results
Prevent growth
Speed migration
Increase plasmids
Select the best answer for each scenario. Quality control technician role?
Design experiments
Test product safety/consistency
Treat patients
Manage data
Select the best answer for each scenario. Unlabeled samples result in:
Faster work
Data loss/reliability issues
Increased purity
Better accuracy
Select the best answer for each scenario. Sterile airflow equipment?
Autoclave
Biosafety cabinet
Incubator
Centrifuge
Select the best answer for each scenario. DNA floats out of well. What helps next time?
Loading dye
Higher voltage
Thicker gel
Longer run
Select the best answer for each scenario. Main GMO ethical concern?
Cost
Environmental/food safety
Equipment access
Speed
Select the best answer for each scenario. DNA sequence analysis using software is:
Bioinformatics
Microbiology
Biochemistry
Manufacturing
Select the best answer for each scenario. Professional lab behavior?
Clean/store equipment
Skip checks
Leave samples
Share unverified data
Select the best answer for each scenario. Which variable is intentionally changed?
Dependent
Independent
Constant
Random
Select the best answer for each scenario. Which tool separates liquids by density?
Incubator
Centrifuge
Autoclave
Freezer
Select the best answer for each scenario. Which molecule carries DNA instructions?
ATP
mRNA
tRNA
rRNA
Select the best answer for each scenario. Why wear gloves during experiments?
Look professional
Prevent contamination/exposure
Improve grip only
Speed work
Select the best answer for each scenario. Which chart shows trends over time?
Pie
Bar
Line
Table
Select the best answer for each scenario. Which organism is used in fermentation?
Virus
Yeast
Bacteria only
Worm
Select the best answer for each scenario. Function of ribosomes?
DNA storage
Protein synthesis
Energy release
Cell movement
Select the best answer for each scenario. Which process copies DNA?
Replication
Translation
Transcription
Mutation
Select the best answer for each scenario. What does a spectrophotometer measure?
Weight
Light absorbance
Temperature
pH
Select the best answer for each scenario. Which base pairs with adenine?
Cytosine
Thymine (DNA)
Guanine
Uracil (RNA)
Select the best answer for each scenario. Which field focuses on soil and water cleanup?
Environmental biotech
Medical biotech
Therapeutic biotech
Health informatics
Select the best answer for each scenario. Which career diagnoses disease through lab testing?
Genetic counselor
Medical lab technician
Bioethicist
Pharmacologist
Select the best answer for each scenario. Which piece sterilizes tools using steam/pressure?
Autoclave
Freezer
Incubator
Oven
Select the best answer for each scenario. What is a phenotype?
Observable traits of an organism
Complete set of genes carried by an organism
A single protein produced by a gene
Conditions present in the growth environment
Which cell type lacks a nucleus?
Plant
Animal
Prokaryotic
Eukaryotic
Why use a negative control?
Confirm no reaction
Show expected outcome
Increase samples
Change variables
What is ligation?
Cutting DNA
Joining DNA fragments
Copying DNA
Separating samples
Which tool views bacteria?
Microscope
Balance
Pipette
pH meter
Which PPE protects eyes?
Mask
Goggles
Gloves
Coat
Which field designs medical devices?
Engineering biotech
Health informatics
Support services
Therapeutic
What is the purpose of a buffer?
Add color
Maintain pH
Increase heat
Stop reaction
Which step comes first in scientific method?
Hypothesis
Observation
Experiment
Conclusion
Which molecule stores cellular energy?
DNA
RNA
ATP
Enzyme
What does GMO stand for?
Genetically Modified Organism
Genetic Molecular Object
General Mutation Option
Growth Model Organism
What does an incubator provide?
Cold storage
Controlled temperature
Sterile airflow
DNA separation
Which role studies disease spread?
Epidemiologist
Geneticist
Microbiologist
Pharmacist
Which lab section explains why the experiment was done?
Results
Introduction
Procedure
Conclusion
Which lab error could cause false results?
Clean equipment
Contaminated reagents
Proper controls
Accurate labeling
Which chart best compares categories?
Line
Pie
Bar
Scatter
Which behavior supports lab safety?
Eating in lab
Wearing PPE
Open shoes
Loose hair
Which job maintains lab instruments?
Lab technician
Geneticist
Bioethicist
Research director
What is transcription?
DNA → RNA
RNA → Protein
DNA → Protein
Protein → RNA
Which symbol shows biohazard?
Flame
Three circles
Skull
Lightning
What is transformation in biotech?
Cell division
DNA uptake by bacteria
DNA replication
Protein synthesis
Which variable is measured?
Independent
Dependent
Constant
Control
Which tool measures microliters?
Burette
Micropipette
Beaker
Flask
Why label samples?
Save time
Prevent mix-ups
Increase volume
Speed reactions
Which field focuses on vaccine development?
Medical biotech
Environmental biotech
Agricultural biotech
Industrial biotech
Which process removes solids from liquids?
Filtration
Freezing
Heating
Mixing
What is the main goal of biotechnology?
Study chemicals
Use living systems to create solutions
Replace biology
Eliminate research
Which of the following are ways that Biotechnology can be used?
Diagnosis of disease
Gene therapy
Production of pharmaceuticals (medicine)
DNA profiling
Agriculture - creating GMOS (Genetically modified organisms)
Look at the image and choose the correct name...
DNA ligases
Recombinant DNA
Restriction enzymes
Transfer vectors
Gel electrophoresis enables scientists to
separate DNA fragments.
combine DNA fragments.
count the genes in DNA.
insert DNA in cells.
List the stages of genetic engineering in the correct order
Cloning , Isolation , Cutting , Expression
Isolation , Cutting , Cloning , Expression
Expression , Cloning , Isolation , Cutting
Cloning , Isolation , Expression , Ligation
A product of genetic engineering technology is represented below . Which substance was needed to join the insulin gene to the bacterial DNA as shown?
a specific carbohydrate
a specific enzyme
hormones
antibodies
What is the result of step 3?
a new type of molecular base is formed
different types of minerals are joined together
DNA from the bacterial cell is cloned
DNA from different organisms is joined together
