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Biotechnology Practice Exam — Applications of Biotechnology

Total questions: 68

Worksheet time: 34mins

Name
Class
Date
1.

Select the best answer for each scenario. A student is preparing agarose gel for DNA separation. The gel solidifies before being poured. What should be done next?

a)

Add buffer

b)

Reheat until liquid

c)

Add DNA

d)

Increase voltage

2.

Select the best answer for each scenario. No colonies grow on a transformation plate. What should be checked first?

a)

Incubation time

b)

Antibiotic activity and plasmid addition

c)

Gel concentration

d)

Plate labeling

3.

Select the best answer for each scenario. A culture spills on the bench. What is the correct response?

a)

Dry wipe

b)

Leave it

c)

Disinfect and follow biohazard cleanup

d)

Rinse with water

4.

Select the best answer for each scenario. Which tool transfers 20 µL accurately?

a)

Beaker

b)

Micropipette

c)

Dropper

d)

Cylinder

5.

Select the best answer for each scenario. PCR fails to amplify DNA. Most likely cause?

a)

Missing primers

b)

Too much agarose

c)

Gel run too long

d)

Extra buffer

6.

Select the best answer for each scenario. Which control confirms bacteria can grow normally?

a)

Plate without antibiotic

b)

Plate with antibiotic

c)

Plasmid plate

d)

Gel lane

7.

Select the best answer for each scenario. A lab report lacks interpretation of results. What is missing?

a)

Title

b)

Procedure

c)

Discussion

d)

Data

8.

Select the best answer for each scenario. A company wants to reduce chemical waste. Which biotech field applies?

a)

Medical

b)

Industrial

c)

Agricultural

d)

Therapeutic

9.

Select the best answer for each scenario. A pipette tip touches the bench before dispensing. Biggest risk?

a)

Evaporation

b)

Contamination

c)

Tip damage

d)

Volume change

10.

Select the best answer for each scenario. Which practice improves data reliability?

a)

Changing variables

b)

Consistent measurements

c)

Skipping controls

d)

Recording final data only

11.

Select the best answer for each scenario. DNA bands smear on a gel. Likely cause?

a)

Overloading sample

b)

Fresh buffer

c)

Correct voltage

d)

New tray

12.

Select the best answer for each scenario. Which document lists chemical hazards and handling?

a)

Equipment log

b)

SDS

c)

Lab manual

d)

Checklist

13.

Select the best answer for each scenario. Wrong incubator temperature may:

a)

Improve results

b)

Prevent growth

c)

Speed migration

d)

Increase plasmids

14.

Select the best answer for each scenario. Quality control technician role?

a)

Design experiments

b)

Test product safety/consistency

c)

Treat patients

d)

Manage data

15.

Select the best answer for each scenario. Unlabeled samples result in:

a)

Faster work

b)

Data loss/reliability issues

c)

Increased purity

d)

Better accuracy

16.

Select the best answer for each scenario. Sterile airflow equipment?

a)

Autoclave

b)

Biosafety cabinet

c)

Incubator

d)

Centrifuge

17.

Select the best answer for each scenario. DNA floats out of well. What helps next time?

a)

Loading dye

b)

Higher voltage

c)

Thicker gel

d)

Longer run

18.

Select the best answer for each scenario. Main GMO ethical concern?

a)

Cost

b)

Environmental/food safety

c)

Equipment access

d)

Speed

19.

Select the best answer for each scenario. DNA sequence analysis using software is:

a)

Bioinformatics

b)

Microbiology

c)

Biochemistry

d)

Manufacturing

20.

Select the best answer for each scenario. Professional lab behavior?

a)

Clean/store equipment

b)

Skip checks

c)

Leave samples

d)

Share unverified data

21.

Select the best answer for each scenario. Which variable is intentionally changed?

a)

Dependent

b)

Independent

c)

Constant

d)

Random

22.

Select the best answer for each scenario. Which tool separates liquids by density?

a)

Incubator

b)

Centrifuge

c)

Autoclave

d)

Freezer

23.

Select the best answer for each scenario. Which molecule carries DNA instructions?

a)

ATP

b)

mRNA

c)

tRNA

d)

rRNA

24.

Select the best answer for each scenario. Why wear gloves during experiments?

a)

Look professional

b)

Prevent contamination/exposure

c)

Improve grip only

d)

Speed work

25.

Select the best answer for each scenario. Which chart shows trends over time?

a)

Pie

b)

Bar

c)

Line

d)

Table

26.

Select the best answer for each scenario. Which organism is used in fermentation?

a)

Virus

b)

Yeast

c)

Bacteria only

d)

Worm

27.

Select the best answer for each scenario. Function of ribosomes?

a)

DNA storage

b)

Protein synthesis

c)

Energy release

d)

Cell movement

28.

Select the best answer for each scenario. Which process copies DNA?

a)

Replication

b)

Translation

c)

Transcription

d)

Mutation

29.

Select the best answer for each scenario. What does a spectrophotometer measure?

a)

Weight

b)

Light absorbance

c)

Temperature

d)

pH

30.

Select the best answer for each scenario. Which base pairs with adenine?

a)

Cytosine

b)

Thymine (DNA)

c)

Guanine

d)

Uracil (RNA)

31.

Select the best answer for each scenario. Which field focuses on soil and water cleanup?

a)

Environmental biotech

b)

Medical biotech

c)

Therapeutic biotech

d)

Health informatics

32.

Select the best answer for each scenario. Which career diagnoses disease through lab testing?

a)

Genetic counselor

b)

Medical lab technician

c)

Bioethicist

d)

Pharmacologist

33.

Select the best answer for each scenario. Which piece sterilizes tools using steam/pressure?

a)

Autoclave

b)

Freezer

c)

Incubator

d)

Oven

34.

Select the best answer for each scenario. What is a phenotype?

a)

Observable traits of an organism

b)

Complete set of genes carried by an organism

c)

A single protein produced by a gene

d)

Conditions present in the growth environment

35.

Which cell type lacks a nucleus?

a)

Plant

b)

Animal

c)

Prokaryotic

d)

Eukaryotic

36.

Why use a negative control?

a)

Confirm no reaction

b)

Show expected outcome

c)

Increase samples

d)

Change variables

37.

What is ligation?

a)

Cutting DNA

b)

Joining DNA fragments

c)

Copying DNA

d)

Separating samples

38.

Which tool views bacteria?

a)

Microscope

b)

Balance

c)

Pipette

d)

pH meter

39.

Which PPE protects eyes?

a)

Mask

b)

Goggles

c)

Gloves

d)

Coat

40.

Which field designs medical devices?

a)

Engineering biotech

b)

Health informatics

c)

Support services

d)

Therapeutic

41.

What is the purpose of a buffer?

a)

Add color

b)

Maintain pH

c)

Increase heat

d)

Stop reaction

42.

Which step comes first in scientific method?

a)

Hypothesis

b)

Observation

c)

Experiment

d)

Conclusion

43.

Which molecule stores cellular energy?

a)

DNA

b)

RNA

c)

ATP

d)

Enzyme

44.

What does GMO stand for?

a)

Genetically Modified Organism

b)

Genetic Molecular Object

c)

General Mutation Option

d)

Growth Model Organism

45.

What does an incubator provide?

a)

Cold storage

b)

Controlled temperature

c)

Sterile airflow

d)

DNA separation

46.

Which role studies disease spread?

a)

Epidemiologist

b)

Geneticist

c)

Microbiologist

d)

Pharmacist

47.

Which lab section explains why the experiment was done?

a)

Results

b)

Introduction

c)

Procedure

d)

Conclusion

48.

Which lab error could cause false results?

a)

Clean equipment

b)

Contaminated reagents

c)

Proper controls

d)

Accurate labeling

49.

Which chart best compares categories?

a)

Line

b)

Pie

c)

Bar

d)

Scatter

50.

Which behavior supports lab safety?

a)

Eating in lab

b)

Wearing PPE

c)

Open shoes

d)

Loose hair

51.

Which job maintains lab instruments?

a)

Lab technician

b)

Geneticist

c)

Bioethicist

d)

Research director

52.

What is transcription?

a)

DNA → RNA

b)

RNA → Protein

c)

DNA → Protein

d)

Protein → RNA

53.

Which symbol shows biohazard?

a)

Flame

b)

Three circles

c)

Skull

d)

Lightning

54.

What is transformation in biotech?

a)

Cell division

b)

DNA uptake by bacteria

c)

DNA replication

d)

Protein synthesis

55.

Which variable is measured?

a)

Independent

b)

Dependent

c)

Constant

d)

Control

56.

Which tool measures microliters?

a)

Burette

b)

Micropipette

c)

Beaker

d)

Flask

57.

Why label samples?

a)

Save time

b)

Prevent mix-ups

c)

Increase volume

d)

Speed reactions

58.

Which field focuses on vaccine development?

a)

Medical biotech

b)

Environmental biotech

c)

Agricultural biotech

d)

Industrial biotech

59.

Which process removes solids from liquids?

a)

Filtration

b)

Freezing

c)

Heating

d)

Mixing

60.

What is the main goal of biotechnology?

a)

Study chemicals

b)

Use living systems to create solutions

c)

Replace biology

d)

Eliminate research

61.
When humans use biological processes to solve a problem, they are using______.
a)
biochemistry
b)
biotechnology
c)
molecular biology
d)
gene therapy
62.

Which of the following are ways that Biotechnology can be used?

a)

Diagnosis of disease

b)

Gene therapy

c)

Production of pharmaceuticals (medicine)

d)

DNA profiling

e)

Agriculture - creating GMOS (Genetically modified organisms)

63.
Artificially changing the genetic material of a cell.
a)
Amino Acid
b)
Nucleic Acid
c)
Phenotype 
d)
Genetic Engineering
64.

Look at the image and choose the correct name...

a)

DNA ligases

b)

Recombinant DNA

c)

Restriction enzymes

d)

Transfer vectors

65.

Gel electrophoresis enables scientists to

a)

separate DNA fragments.

b)

combine DNA fragments.

c)

count the genes in DNA.

d)

insert DNA in cells.

66.

List the stages of genetic engineering in the correct order

a)

Cloning , Isolation , Cutting , Expression

b)

Isolation , Cutting , Cloning , Expression

c)

Expression , Cloning , Isolation , Cutting

d)

Cloning , Isolation , Expression , Ligation

67.

A product of genetic engineering technology is represented below . Which substance was needed to join the insulin gene to the bacterial DNA as shown?

a)

a specific carbohydrate

b)

a specific enzyme

c)

hormones

d)

antibodies

68.

What is the result of step 3?

a)

a new type of molecular base is formed

b)

different types of minerals are joined together

c)

DNA from the bacterial cell is cloned

d)

DNA from different organisms is joined together