Review PCR and Gel Electrophoresis

Front

What is the purpose of gel electrophoresis?

Back

Gel electrophoresis separates DNA based on size.

Front

What are the building blocks of DNA?

Back

The building blocks of DNA are nucleotides.

Front

What happens to DNA fragments during gel electrophoresis?

Back

Smaller DNA fragments move faster through the gel than larger fragments.

Front

What laboratory procedure is used to separate DNA fragments?

Back

The laboratory procedure used is gel electrophoresis.

Front

What is the role of agarose gel in gel electrophoresis?

Back

Agarose gel acts as a medium that allows the separation of DNA fragments based on size.

Front

What is PCR (Polymerase Chain Reaction)?

Back

PCR is a technique used to amplify small segments of DNA.

Front

What are the main steps of PCR?

Back

The main steps of PCR are denaturation, annealing, and extension.

Front

What enzyme is commonly used in PCR?

Back

Taq polymerase is commonly used in PCR.

Front

How does gel electrophoresis visualize DNA?

Back

Gel electrophoresis visualizes DNA using a dye that binds to DNA and fluoresces under UV light.

Front

What is the significance of the DNA ladder in gel electrophoresis?

Back

The DNA ladder serves as a size reference for comparing the lengths of DNA fragments.