4GENBIO2 - GENETIC ENGINEERING

Multiple Choice

Which technique involves the use of molecular methods to modify the traits of a target organism?

• Genetic engineering

• Selective breeding

• Biotechnology

• Natural selection

Open Ended

Explain how selective breeding can lead to increased yield and quality of crops over several generations.

Multiple Select

Which environmental pressures can plants be engineered to tolerate?

• Salinity

• Extreme temperature

• Drought

• Low light

Multiple Select

Which of the following are improved plant characteristics that can result from genetic engineering or selective breeding?

• Increased crop yield

• Resistance to viruses

• Tolerance to drought

• Increased tolerance to insect pests

Multiple Choice

Which plant characteristic was improved as shown in the first image, and how was this improvement measured?

• Increased tolerance to herbicides, measured by plant survival and seed production after spray

• Increased drought resistance, measured by leaf color

• Faster growth rate, measured by plant height

• Larger fruit size, measured by fruit weight

Multiple Choice

Arrange the steps for making recombinant DNA in the correct order based on the images.

• Cut the plasmid using restriction enzymes, seal the foreign DNA with ligase, return plasmid to bacterial cell

• Seal the foreign DNA with ligase, cut the plasmid using restriction enzymes, return plasmid to bacterial cell

• Return plasmid to bacterial cell, cut the plasmid using restriction enzymes, seal the foreign DNA with ligase

• Cut the plasmid using restriction enzymes, return plasmid to bacterial cell, seal the foreign DNA with ligase

Multiple Choice

Which process allows bacterial cells to replicate a desirable gene and produce substances like insulin?

• Gene gun biolistics

• Gene cloning

• Electroporation

• Heat shock treatment

Multiple Choice

Which of the following methods can be used to insert plasmids into cells?

• Gene gun biolistics

• Heat shock treatment

• Electroporation

• All of the above

Open Ended

Describe two reasons why amplifying DNA is important in scientific research.

Open Ended

Which process involved in genetic engineering do you find most interesting, and why?