BIOTECHNOLOGY-rDNA technology-grade XII

Which of the following would you choose as safest and least cumbersome selectable marker

• ampicillin resistant gene

• beta galactosidase resistant gene

• tetracycline resistant gene

• kanamycin resistant gene

If you want to isolate a particular gene from a plasmid or genome of an organism which of the following enzymes has to be used

• DNA polymerase

• Lysozyme

• DNA ligase

• restriction enzymes

Arrange the following enzymes/process in the order of their use for a certain experiment in genetic engineering.

1.Ligase

2.Restriction enzymes

3.Desired gene

4.Transformation

5.Expression.

• 3-2-1-4-5

• 1-2-4-5-3

• 3-1-4-5-2

• 2-5-4-3-1

In the year 1963,the two enzymes responsible for inhibiting the growth of bacteriophage in E.coli were isolated

• polymerase

• ligase

• antibiotic resistance enzymes

• molecular scissors

In a DNA gel the fragments of 2kb and 3kb will be

• 2kb nearer to cathode and 3kb nearer to anode.

• 2kb closer to the wells while 3kb towards the opposite ends

• 2kb towards the opposite ends of gel and 3kb closer to the wells

• 2kb away from cathode while 3kb towards anode

If the beta galactosidase gene is not activated, means that there will be color in the colonies of

• Non- recombinants

• Recombinants

• Non- transformants

• Transformants

If you start with 10 molecules of dsDNA, what will be the number of DNA molecules after 10 cycles of PCR.

• 10240

• 512

• 5120

• 1024

Biolistic method makes use of microparticles coated with DNA bombarded at cells to be transformed. These particles are made up of

• selenium or platinum

• silicon or gold

• tungsten or gold

• zinc or tungsten

The microbial source for restriction enzyme PstI is

• Pseudomonas stuarii

• Propionibacerium stuarii

• Providencia stuartii

• Pseudomonas salmon

The term for replacing a defective mutant allelle with a functional allelle

• Gene amplification

• Gene splicing

• Gene therapy

• Gene mutation