MBT Restriction Enzymes & PCR

The DNA base sequences recognized by Endonuclease are called

• Restriction Site

• Recognition Site

• Fragmentation Site

• Cutting Site

How do we name Restriction Enzymes ?

• First letter in Cap for Genus, next two letter in small for species, next one letter for strain and roman numeral for order of discovery

• First two letter in Cap for Genus, next one letter in small for species, next one letter for strain and English numeral for order of discovery

• First two letter in Cap for Genus, next one letter in capital for species, next one letter for strain and roman numeral for order of discovery

• First two letter in Cap for Genus, next one letter in small for species, next one letter for strain and roman numeral for order of discovery

Restriction Enzymes were primarily used as :

• Defense by Protozoa against antibiotics

• Tool by Virus to cleave Bacterial genome

• Defense by Bacteria against bacteriophages viruses

• All of the above

Which RE's from above make blunt cuts?

• Alul

• HaeIII

• BamHI

• HindIII

• EcorRI

Which RE's from above make sticky cuts?

• Alul

• HaeIII

• BamHI

• HindIII

• EcorRI

If a restriction enzyme cuts linear DNA 3 times, how many DNA fragments were made?

• 1

• 2

• 3

• 4

______ seals together fragments of DNA through phosphodiester bonds.

• Restriction Endonucleases

• DNA Ligase

• Recognition sites

• Viral DNA

When creating a recombinant plasmid, why are many copies of the plasmid made?

• Because the plasmid has been amplified through PCR

• Because recombinant plasmids are made by bacteria in response to a viral attack

• Because many recombinant plasmids will not correctly take up the target DNA

• Because the DNA will be cut into restriction fragments

If a restriction enzyme cuts a plasmid 3 times, how many DNA fragments are made?

• 1

• 2

• 3

• 4

What is NOT needed for a PCR reaction?

• DNA template

• Taq polymerase

• Nucleosides

• Primase