Gel Electrophoresis Basics Worksheet

Each band in a DNA electrophoresis gel is made up of one molecule of DNA.

• True

• False

Gel Electrophoresis can tell you the sequence of a particular DNA fragment.

• True

• False

You can see DNA on a gel because DNA is naturally fluorescent.

• True

• False

DNA moves through a gel because it is negatively charged and is attracted to the positive electrode.

• True

• False

The speed at which DNA moves through a gel is diretly related to its size.

• True

• False

An electrophoresis gel used for DNA is usually made from agarose which is a protein obtained from seaweed.

• True

• False

When visualizing your gel, you can tell the size of the DNA fragments by seeing how wide each band is.

• True

• False

A gel is placed in a liquid called running buffer because it is an insulator and will portect the user from electric shock.

• True

• False

Which DNA fragment, A, B, C, D, E, F is the largest?

How do you know when the DNA fragment is the largest?

• It travels the furthest down the gel.

• It is the closest to the wells.

• It has the darkest band.

• It has the thickest band.