Exploring Recombinant DNA Technology

What is one of the primary uses of recombinant DNA technology?

• To create new species

• To develop vaccines

• To destroy harmful bacteria

• To increase plant growth

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NGSS.HS-LS1-1

What is the function of restriction enzymes in genetic engineering?

• To replicate DNA

• To cut DNA at specific sequences

• To join DNA fragments

• To mutate DNA

Which type of restriction enzyme cut leaves jagged edges known as sticky ends?

• HaeIII

• EcoRI

• Both EcoRI and BamHI

• BamHI

What are plasmids?

• Rings of DNA in bacteria

• Linear DNA strands in bacteria

• Proteins in cells

• Enzymes that cut DNA

How is a gene of interest inserted into a plasmid?

• By heating the plasmid

• By cutting the plasmid with the same restriction enzyme

• By using a virus

• By using a different restriction enzyme

What role does DNA ligase play in creating recombinant DNA?

• It mutates the DNA

• It acts as glue to join DNA fragments

• It replicates the DNA

• It cuts the DNA

What is the result of a successful ligation in recombinant DNA technology?

• A recombinant plasmid

• A self-ligated plasmid

• A destroyed plasmid

• An inactive plasmid

What happens if a plasmid undergoes self-ligation?

• The gene of interest is expressed

• The plasmid is destroyed

• The gene of interest is not inserted

• The plasmid becomes inactive

What is an inversion in the context of recombinant DNA technology?

• When the plasmid is cut incorrectly

• When the gene of interest is inserted backwards

• When the plasmid is not cut

• When the gene of interest is not expressed

How does recombinant DNA technology impact our daily lives?

• By increasing plant growth

• By destroying harmful bacteria

• By developing vaccines and medications

• By creating new species

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