GPC 2

Which of the following is essential for the separation of compounds in chromatography?

• Large band broadening

• Differences in retention of compounds

• Identical retention of compounds

• Elution without peak separation

What is the term used to describe the relative rate at which a solute migrates through a chromatographic column?

• Retention time

• Capacity factor (k)

• Elution rate

• Separation factor

Which of the following retention factor (k) values is considered optimal for chromatography separation?

• k < 1

• k > 20-30

• 2 < k < 10

• k = 1

Which of the following is a correct statement about retention time (tR)?

• It is affected by the flow rate of the mobile phase.

• It is the total time required for the mobile phase to pass through the column.

• It is the time taken for a solute to reach the detector.

What is the formula for calculating the plate height (H) in chromatography?

• H=L/N​

• H=N/L

• H=L/σ2​

• H=σ/N​

What is the purpose of determining the theoretical plate number (N) in chromatography?

• To calculate retention factor (k)

• To determine column length (L)

• To measure the efficiency of separation

• To calculate the distribution constant (KD)

Which of the following is true for the separation factor (α) in chromatography?

• It measures the rate of elution.

• It must be greater than 1 for separation to occur.

• It is always less than 1.

• It is used to calculate the plate count (N).

Which of the following correctly describes the relationship between plate count (N) and efficiency of a chromatographic system?

• Higher N indicates lower efficiency.

• Higher N indicates better separation and efficiency.

• Lower N means better separation and efficiency.

• N is unrelated to the efficiency of a chromatographic system.

How is the peak resolution (Rs) calculated in chromatography?

• By measuring the width of the peak at half height.

• By using the difference in retention time and the sum of baseline widths of two peaks.

• By dividing the column length (L) by the plate count (N).

• By calculating the distribution constant (KD).

What does a tailing factor (T) of 1.0 indicate in chromatography?

• The peak is symmetrical.

• The peak has a trailing edge.

• The peak is broadening.

• The peak has an asymmetry.