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WorksheetsBioscience ADE Technical Assessment Review
Total questions: 120
Worksheet time: 3600secs
Identify the following piece of laboratory glassware:
Flask
Beaker
Graduated Cylinder
Florence Flask
Identify the following piece of laboratory glassware:
Beaker
Erlenmeyer Flask
Graduated Cylinder
Florence Beaker
Identify the following piece of laboratory glassware:
Volumetric Flask
Beaker
Erlenmeyer Flask
Graduated Cylinder
What is the most proper procedure for the handling of broken laboratory glassware?
Notify the laboratory technician so that they can come clean it up.
Use the designated dust pan and brush to sweep up the glass pieces, then dispose of it in the container labeled "Broken Glass".
You do not dispose of your own broken glass. You collect the pieces and inform the tech so it can be fixed.
Try to reuse the items that has been broken because glassware is very expensive.
It depend on how broken the glassware is. Small cracks do not matter but larger ones require disposal of the glass.
Identify the following piece of laboratory glassware:
Crucible
Petri Dish
Watch Glass
Contact Lense
Identify the following piece of laboratory glassware:
Glass Rod
Pipette
Test Tube
Syringe
Why is it necessary to clean all materials and glassware after working in the lab?
Dirty materials and glassware will start to smell bad and will forever contain a unique odor when using.
Chemical residue left on the materials and glassware may affect later experiments or cause a chemical reaction.
You actually do not need to wash your materials or glassware after lab as long as you remember to do it before the next lab.
Some chemicals in the lab may slowly dissolve glass, and if you leave them long enough holes will form.
Which piece of laboratory glassware should never be used to mix or dissolve any solutes?
Wheaton Bottle
Erlenmeyer Flask
Beaker
Graduated Cylinder
Which piece of glassware is designed to easily mix, seal, and pour solutions due to its wide body and narrow neck?
Erlenmeyer Flask
Beaker
Graduated Cylinder
Glass Pipette
Watch Glass
Which piece of glassware is used to hold, mix, and heat solutions; but generally is not used for storage of prepared solutions?
Serological Pipette
Volumetric Flask
Cylinder
Flask
Beaker
Which piece of glassware is not usually used to heat solutions in?
Beaker
Graduated Cylinder
Wheaton Bottle
Flask
True or False: Before working with glassware, always inspect it for flaws and defects. Glass should be removed from service if damage is discovered.
True
False
Select all of the following that are correct when handling and storing glassware safely:
Never carry a flask by its neck.
Never carry a beaker by its side.
Carry wet glassware upside down to allow moisture of evaporate while carrying.
Always use two hands carrying any glassware; one hand under the glass for support.
When handling hot or cold glassware, always wear insulated gloves.
Select all of the following that are correct when handling and storing glassware safely:
Keep laboratory glassware away from shelf edges.
Place glassware towards the back of benches or hoods.
Place glassware towards the front of benches or hoods for quick access.
Don't let glass instruments roll around in drawers.
Identify the following piece of laboratory equipment/instrumentation:
Table Top Balance
Analytical Balance
Spring Balance
Triple-Beam Balance
Identify the following piece of laboratory equipment/instrumentation:
Spring Balance
Triple-Beam Balance
Table Top Balance
Analytical Balance
Dry solutes should never be directly placed on a balance. What should be used when weighing dry solutes?
Petri Dish
Scale Plate
Chemical Scoop
Weigh Boat/Paper
Which one of the following is a unit of measurement used by a laboratory balance?
gram
meter
liter
kiloliter
milliliter
Which one of the following is a unit of measurement used by a laboratory balance?
meter
milligram
liter
deciliter
milliliter
What must you never do when working with a balance?
Check balance for damaged glass and power cords prior to use.
Clean the scale plate and surroundings with a brush before use.
Press down on the scale plate of the balance.
Press the zero/tare button when you have finished weighing solutes.
Identify the instrument in the biotechnology lab that is used to measure mass.
Beaker
Pipette
Balance
Flask
Weigh Boat
What is the proper way to negate the mass that a weigh boat contains so that you can measure chemicals in the lab most accurately?
Do not use a weigh boat but instead measure directly on the scale plate of the balance.
Place the weigh boat on the scale and write down the mass of the weigh boat.
Place the weigh boat on the scale. Proceed to measure out the solute and then add 0.05g more of solute.
Place the weigh boat on the scale and press the zero/tare button.
Which type of balance should be used to mass an object to produce an error smaller than 0.01g?
Table Top Balance
Triple-Beam Balance
Analytical Balance
Spring Balance
Which type of balance is used in standard preparative work when an error of +/- 0.02g is acceptable?
Table Top Balance
Analytical Balance
Spring Balance
Triple-Beam Balance
This piece of glassware is used to measure exactly 50 ml of liquid with a high degree of accuracy.
50 ml Beaker
50 ml volumetric flask
100 ml Erlenmeyer flask
Pasteur pipette
Which of the following is an appropriate piece of glassware to use when measuring 12 mls of ethanol?
100 ml beaker
25 ml graduated cylinder
50 ml graduated cylinder
25 ml volumetric flask
You should always use a __________ what heating a solution on a hot plate.
Fume hood
Bunsen burner
Centrifuge
Thermometer
Identify the following piece of laboratory equipment/instrumentation:
pH Meter
Hot plate/stirrer
Table Top Balance
Shaker
The following safety symbol is indicating what type of warning?
Thermometer required
Low temperature
High temperature
Fire danger
Before opening a centrifuge that just ran you should:
Tell your instructor
Insure the load has completely stopped
Insure tubes are labeled and secured
Count all of the tubes.
What is one common use for and incubator in a lab?
Dry glassware
Grow cultures
Clean glassware
Separate solids
What is the optimal growth temperature for the model organism Escherichia coli to incubate at?
27°C
25°C
37°C
40C
This piece of equipment is used to sterilize glassware in a laboratory:
Dishwasher
Centrifuge
Incubator
Autoclave
When trying to evacuate noxious exhaust from a reaction or experiment what lab equipment should be used?
Fan
Fume Hood
Laminar Flow Hood
Open Window
As absorbance goes up, transmittance
Remains the same
Doubles
Goes up
Goes down
The official piece of lab equipment that is intended for testing samples in a spectrometer is:
Test Tube
Beaker
Cuvette
Scoopula
What does PPE stand for?
Protection Protocol Equipment
Preventative Protocol Equipment
Personal Protective Equipment
Personal Protocol Experiment
Which piece of Equipment should be put on without contaminating the outer surface?
Goggles
Gloves
Apron
Lab Coat
Which of the following would not be good examples of wearing proper PPE?
Wearing an unbuttoned lab coat
Wearing safety glasses over prescription glasses
Wearing gloves that are one size to big
Wearing hiking boots
Safety equipment is often issued to help employees in protecting themselves from hazards of their __________________.
home environments
school environments
toxic environments
Work environments
In the event that a lab coat is not available, a student can request to use a(an) _______________ to protect their clothing during a lab experiment.
art smock
fire blanket
lab apron
nothing needs to be requested
Which of the following would not be good examples of wearing proper PPE?
goggles that cover the eyes and strap adjusted to fit
hair pulled up in a high pony tail
wearing gloves that have the fingers ripped off
wearing rubber rain boots
Which biosafety level characterizes most high school laboratories?
Level 4
Level 1
Level 2
Level 3
Which biosafety level would be used to characterize a facility that contains the small pox virus?
Level 1
Level 2
Level 3
Level 4
Where do you look to find information about a chemical you may be working with?
Occupational Safety and Health Administration
Hazardous Communication System
Globally Harmonized System
Safety Data Sheet
What should you do every time you finish a lab?
Wash your hands
Tie your hair back
Check out with your instructor
Turn in your lab work
Whenever a spill occurs the first thing you should do is what?
Wash your hands
Tell your neighbors
Notify your instructor
Clean it up
What is the most common type of lab spill incident size in a school?
more than 10 liters
5 to 10 liters
less than a liter
a liter to 5 liter
Which of the following is the primary role of the Food and Drug Administration (FDA)?
To create quality systems for each type of drug industry and insure compliance.
To pass laws that determine where food and drugs can be produced.
To test food and drug products to determine if they are ready to be sold to consumers.
To regulate and monitor the production, use and testing of drugs.
Which of the following documentation types is the most appropriate to use when preparing to make a solution?
An equipment operation procedure
A standard operation procedure
A calibration log
A material specification sheet
Which of the following is the primary use of the first stop on a micropipettor plunger?
To insure that an accurate amount of material is loaded into the micropipettor.
To insure that the correct sized micropipettor is being used.
To insure that the correct sized pipette tip is being used.
To insure that an accurate amount of material is dispensed from the micropipettor.
When are micropipettors used to measure liquids?
For liquid volumes of any size
For liquid volumes of 1 ml or less
For liquid volumes of 1µl or less
For liquid volumes of 1L or less
Why is DNA, deoxyribonucleic acid, an important molecule in modern science?
Is found everywhere on earth.
Is found in all things on earth.
Is found in every known living organism on earth.
Is found throughout the universe.
Which of the following statements accurately describes the function of an SOP document?
An SOP provides a guideline that can be modified to suit the needs of a researcher
An SOP provides a variety of methods that allow a researcher to choose the best method to produce product.
An SOP provides a list of materials that could be used to produce a product.
An SOP provides the accepted method to complete a process and/or product
Why is having a Quality System an FDA required action?
A Quality System allows a company to manufacture all type of products.
A Quality System meets the government’s duty to insure safe medical products
A Quality System demonstrates a company’s method of making compliant products
A Quality System prevents the FDA from inspecting a company.
Which of the following statements accurately describes the function of Quality Assurance?
A company department that insures that the quality system is followed and maintained
A company department that insures that the quality system is followed and maintained
A company department that develops products using a quality system.
A company department that interacts with government agencies to determine regulations
Which one of the following is NOT a use of bacteriological media?
Provide the nutrients necessary for bacterial growth
Sterilize agar plates against the growth of mold
Allow for the isolation of specific bacteria
Stimulate the growth of a large amount of bacteria in a short amount of time
Which of these ingredients of Nutrient Agar does not provide a source of energy and nutrition for microbes?
Agar
Peptone
Beef Extract
All of these provide energy and nutrition
The study and manipulation of living things or their component molecules, cells, tissues, or organs.
Biochemistry
Bioinformatics
Biotechnology
Biology
Which of the following is NOT a curdling agent used to make cheese?
Chymosin
Emporase
Rennin
Amylase
Which domain of biotechnology does transgenic plants and animals fall under?
Agricultural Biotechnology
Diagnostic Research Biotechnology
Industrial and Environmental Biotechnology
Medical/Pharmaceutical Biotechnology
Which domain of biotechnology does DNA identification and DNA fingerprinting, forensics falls under?
Agricultural Biotechnology
Diagnostic Research Biotechnology
Industrial and Environmental Biotechnology
Medical/Pharmaceutical Biotechnology
Which domain of biotechnology does the production of insulin fall under?
Agricultural Biotechnology
Diagnostic Research Biotechnology
Industrial and Environmental Biotechnology
Medical/Pharmaceutical Biotechnology
Which domain of biotechnology does fermented foods and beverages fall under?
Agricultural Biotechnology
Diagnostic Research Biotechnology
Industrial and Environmental Biotechnology
Medical/Pharmaceutical Biotechnology
Which of the following is NOT an aspect of setting up a legal scientific lab book
Dating every page
Numbering every page
Maintaining a table of contents
Editing using white out
What is the correct order of the components of a lab report?
Problem/question, hypothesis, material, procedures, data/observations, analysis, conclusion
Hypothesis, problem/question, material, procedures, analysis, data/observations, conclusion
Problem/question, material, hypothesis, procedures, analysis, data/observations, conclusion
Problem/question, data/observations, material, procedures , hypothesis, analysis, conclusion
Which of the following examples would NOT be criteria for an article being reliable?
The author being an expert in the field
The article authored by a scientific organization
The author by an intelligent individual
The author sites the experimental process used and data
What is the best source of information used in scientific research?
An article published in a peer-reviewed science journal.
A blog of a graduate student at a prestigious university
An interview by a news reporter of a Nobel laureate
A Wikipedia article on the topic.
Which of the following sets of key terms would be used to locate relevant articles for a student doing research on protein synthesis?
Nucleotides, rRNA, translation
Amino acids, cytosine, thymine
Amino acids, transcription, mRNA
Nucleotides, cytosine, adenine
Which of the following sets of key terms would be used to locate relevant articles for a student doing research on DNA replication?
Ribosome, transcription, translation
DNA polymerase, deoxyribonucleotides, ligase
RNA polymerase, transcription, DNA polymerase
Leading strand, lagging strand. ribonucleotides
The device that produces magnified images of structures that is too small to see with the unaided eye.
Microscope
Nano Drop
Spectrophotometer
Stereoscope
Testable questions have all of the following characteristics EXCEPT:
They are based on information or observation.
They involve the development of experiment that will isolate specific variables and control what is being tested.
They can produce measurable and comparable numerical results
They always produce a clear answer once tested.
Evaluate the following hypothesis: If hand sanitizer is effective then it will inhibit bacterial growth.
Incorrect: Missing what condition is being tested.
Incorrect: Missing what result is expected.
Incorrect: Missing how the results will be measured.
Correct: All parts of a scientific hypothesis are present.
Which of the following is an appropriate hypothesis if a researcher is comparing the effectiveness of cleaning products?
Which cleaning product will work the best?
If a cleaning product is expensive, then it will be easy to find in stores.
If a cleaning product is less expensive, then it will kill more bacteria as measured by the number of colonies in a petri dish.
If a cleaning product is more expensive, then one could expect to see more antibiotic resistant strains of bacteria over time in areas where it is commonly used.
What would be an appropriate negative control in a PCR reaction where the researcher is looking for the gene for insulin?
A sample without the gene for insulin
A sample with the gene for insulin
A sample with DNA from a human cell
A sample with DNA from a transformed yeast cell
Why are procedures important in science?
They allow a different researcher to repeat the experiment
The scientist can trouble shoot problems
They are part of a legal lab notebook
All the above
Which of the following should NOT be done when streaking a petri dish?
Turn the plate upside down in the incubator
Take the lid off and place it on the table prior to streaking
Write important information of the base of the petri dish
Push the swab into the agar when streaking
You are testing the effect of fertilizer on plant vertical growth. What would be an appropriate quantitative measure of this effect?
Description of plants after the fertilizer is added
Count of the number of leaves on each plant
Description of the color leaves
The height of the plant in cm.
You are testing the effect of fertilizer on plant appearance. What would be an appropriate qualitative measure of this effect?
Description of leaf color and shape
Count of the number of leaves on each plant
measurement in cm of the size of each leaf
The height of the plant in cm.
Where should the independent variable be on the graph?
X axis
Y axis
Z axis
In the key
When dealing with bacteria growth over time, which type of graph is appropriate?
Bar graph
Pie chart
Line graph
Double bar graph
Which of the following should NOT be included in a conclusion?
Discussion of the hypothesis
Possible errors
Numbers
Graph
This is the part of the experimental write up where your data is summarized and possible sources of error are addressed.
Procedures
Backgroun
Conclusion
Backgroun
What should be added to a microbial plate prior to disposal?
Sodium Hydroxide
95% ethanol
50% ethanol
A dilute acid
Where should someone dispose of contaminated plastic ware?
Trash can
Garbage dump
Recycling
Biohazard bag
Which of the following is NOT a common characteristic of model organisms used for lab research?
Large size
Short lifespan
Inexpensive
Easy to use
How is a recombinant plasmid different from a naturally occurring plasmid?
Scientists find recombinant plasmids only in E. coli bacteria
Scientists make recombinant plasmids with foreign genes
Scientists use recombinant plasmids to probe for insect DNA
Scientists do not distinguish between recombinant vs. natural
When selecting a restriction enzyme to create a recombinant plasmid, what should you consider?
The restriction enzyme should cut the plasmid twice
The restriction enzyme should cut the plasmid 5+ times
The restriction enzyme should cut the plasmid only once
The restriction enzyme should not cut the plasmid at all
Genes in plasmids can be turned on and off using inducer molecules. Which of the following would happen if an inducer is present?
The inducer would bind to RNA Polymerase, making it start transcription
The inducer would bind to the repressor, removing it from the operator
The inducer would not bind to anything, because it prevents transcription
The inducer would not bind to the operon, but would bind to the nucleus
Which of the following experiments could be performed using the Lac Operon?
How does ultraviolet light effect the growth of transformed E. coli bacteria
How well is lactose nutritional quality maintained in different temperatures
How much inducer molecule is required to maximize protein synthesis
How many milk products contain sugars that cannot be easily metabolized
Where does replication begin within the DNA of a cell?
Replication Fork
Origins of Replication
Replication Initiating Site
Initiator
Which enzyme is responsible for catalyzing the covalent bonding of the 3’ end of a Okazaki fragment to the 5’ end of a growing chain?
Helicase
Polymerase
Ligase
Primase
In PCR denaturation occurs at what temperature?
37°C
59°C
72°C
92°C
In PCR annealing occurs at what temperature?
37°C
59°C
72°C
92°C
What is the process of amplifying segments of a DNA molecule?
Centrifuging
Electrophorogram
Gel Electrophoresis
PCR
What sequence of nucleotides attaches to the area to be replicated to indicate where nucleotides need to be added?
Origin of Replication
Primers
Terminators
Markers
Which piece of equipment is a PCR performed?
Centrifuge
Heat Bath
Incubator
Thermocycler
What substance can be used to stain DNA in gel electrophoresis?
Iodine
Ethidium Bromide
Safranin O
Congo Red
Why is it important to use extreme care when handling ethidium bromide?
It can stain your clothes
It's a strong acid
It's a strong base
It's a carcinogen
What database is most frequently used by genetic researchers to identify nucleotide sequences?
NCBI
Flybase
Bing
You’re working for a lab that is looking at the human gene for insulin, what would be the best database to use?
NCBI
Flybase
Bing
Given the following two sequences, determine the score for best alignment:
GCGTAA -CGTACT
GCTAAAACGCACT
1
2
3
4
Which of the following is not a type of mutation seen in alignments?
Addition
Deletion
Inversion
Substitution
Which software program compares a nucleotide sequence with other sequences in databases to determine degrees of similarity?
protein blast
nucleotide blast
blastx
tblastn
Which of the following websites would have free access to a vast number of DNA sequence databases?
www.restrictionmapper.org
www.neb.com (New England Biolabs)
www.biomedical_center.org (Harvard University Health Library)
www.ncbi.nlm.nih.gov (National Center for Biotechnology Information)
What tool is available for comparing a specific DNA sequence with all other known similar sequences?
BLAST
X-ray crystallography
Shot-gun cloning
Northern Blot
What website will you need to use if you would like to perform a BLAST of a nucleotide sequence?
www.ncbi.nlm.nih.gov
www.restrictionmapper.org
www.neb.com
www.biomedical_center.org
Which of the following protein assays can be used in combination with a spec 20 to quantify the amount of protein in solutions (Hint: there is a color change from brown to blue)
Benedict’s assay
Lowry assay
Biuret assay
Bradford assay
This term describes a test done in bioscience to determine something about a substance.
Test
Assay
Amstray
Litmus test
Which of the following is created prior to finding the concentration of an unknown protein solution?
Gel electrophoresis gel
ELISA
Biuret Assay
Standard Curve
This type of buffer is often used to perform dilutions in protein based solutions?
Bradford's reagent
TAE
ethanol
PBS
This value on a spectrophotometer will be given between 100% - 0%
Concentration
Absorbance
Transmittance
Filtration
Which of the following statements gives the correct order of the steps of protein synthesis?
mRNA to tRNA to DNA to protein
tRNA to mRNA to DNA to protein
DNA to tRNA to mRNA to protein
DNA to mRNA to tRNA to protein
Where do the two phases of protein synthesis occur in a cell?
Transcription occurs within the nucleus and translation occurs in the cytoplasm.
Termination occurs in the nucleus and initiation occurs in the cytoplasm
Translation occurs within the nucleus and transcription occurs in the cytoplasm.
Initiation occurs in the nucleus and termination occurs in the cytoplasm
When running SDS-PAGE electrophoresis, the sample buffer composition (for example: denatured vs. non-denatured) determines what characteristics of protein structure?
Determines the secondary structure of the alpha helix and beta sheets
Determines the primary structure of the amino acid chains
Minimizes the effects of secondary and tertiary structures to make the protein linear.
Maximizes the effects of secondary and tertiary structures to make the protein LESS linear
A phylogenetic trees is a diagram that shows:
relatedness of species through a series of single, common ancestors
relatedness of species through a series of overlapping ancestors
relatedness of species through observation of similar behaviors alone
relatedness of species through physical attributes alone
Antibodies are referred to as specific because:
all antibodies are the same from species to species.
each antibody has many antigens that it will bind.
all antibodies bind to all antigens.
each antibody has only one antigen that it will bind.
Plate ELISAs are a powerful immunological technique because:
they rapidly and accurately identify specific proteins of interest.
they rapidly transfer proteins from polyacrylamide to nitrocellulose
they rapidly separate specific proteins using charge and size
they rapidly precipitate proteins to concentrate sample for use in protein assays
ELISA stands for :
Enzyme-Linear ImmunoSorbant Assay
Enzyme-Linked ImmunoSorbant Assay
Enzyme-Linked ImmunoSorbant Antibody
Enzyme-Linked ImmunoSolvent Assay
Antibodies are produced to a particular antigen by an organism’s immune system because:
the organism’s immune system produces antibodies in anticipation of being invaded by pathogens
the organism’s immune system recognizes the protein as self
the organism’s immune system produces antibodies whether it is infected or not
the organism’s immune system recognizes the protein as non-self.
