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Microbial Growth

Total questions: 55

Worksheet time: 28mins

Name
Class
Date
1.

Bacteria make necessary enzymes for growth/reproduction.

a)

Lag

b)

Log

c)

Stationary

d)

Death

e)

Long-term Stationary (LTS)

2.

Most binary fission occurs.

a)

Lag

b)

Log

c)

Stationary

d)

Death

e)

Long-term Stationary (LTS)

3.

#New cells = #Dying cells

a)

Lag

b)

Log

c)

Stationary

d)

Death

e)

Long-term Stationary (LTS)

4.

No nutrients or space remain for growth.

a)

Lag

b)

Log

c)

Stationary

d)

Death

e)

Long-term Stationary (LTS)

5.

Spores are formed or new protective genes are activated.

a)

Lag

b)

Log

c)

Stationary

d)

Death

e)

Long-term Stationary (LTS)

6.

Culture media provide microbes with:

a)

CO2

b)

O2

c)

nutrients

d)

agar

7.

Chemicals released by bacteria in a biofilm that allow quorum sensing:

a)

neurotransmitters

b)

chemotaxants

c)

autoinducers

d)

planktonic juices

8.

Bacteria growing in natural environments form:

a)

colonies

b)

pure cultures

c)

spores

d)

biofilms

9.

Bacteria growing in lab environments form:

a)

colonies

b)

gangs

c)

spores

d)

biofilms

10.

The biosafety level (BSL) of most introductory microbiology labs is:

a)

BSL-1

b)

BSL-4

c)

BSL-2

d)

BSL-3

11.

Bacteria communicate cell-to-cell using:

a)

planktonic phrasing

b)

subculturing

c)

quorum sensing

d)

inoculation

12.

A:

a)

obligate aerobe

b)

obligate anaerobe

c)

facultative anaerobe

d)

microaerophile

e)

aerotolerant

13.

B:

a)

obligate aerobe

b)

obligate anaerobe

c)

facultative anaerobe

d)

microaerophile

e)

aerotolerant

14.

C:

a)

obligate aerobe

b)

obligate anaerobe

c)

facultative anaerobe

d)

microaerophile

e)

aerotolerant

15.

D:

a)

obligate aerobe

b)

obligate anaerobe

c)

facultative anaerobe

d)

microaerophile

e)

aerotolerant

16.

E:

a)

obligate aerobe

b)

obligate anaerobe

c)

facultative anaerobe

d)

microaerophile

e)

aerotolerant

17.

Biofilms are associated with more than 50% of infections.

a)

True

b)

False

18.

The two most common types of media used in the laboratory are:

a)

chemically defined & simple

b)

agar & broth

c)

simple & complex

d)

differential & selective

19.

Microbes can be preserved for long periods of time by:

a)

pasteurization

b)

lyophilization

c)

incubation

d)

refrigeration

20.

A single colony:

a)

is grown to provide a pure culture

b)

starts as a single bacterial cell

c)

consists of identical "clones"

d)

all of these

21.

Which of the following are not associated with food spoilage?

a)

none of these cause food spoilage

b)

archaea

c)

hyperthermophiles

d)

psychrophiles

22.

Is H2O2 (hydrogen peroxide) toxic?

a)

No

b)

Yes, it is always toxic

c)

Not if you produce catalase and peroxidase

d)

Not if you produce superoxide dismutase

23.

Fastidious organisms:

a)

are motile

b)

are anaerobic

c)

require many growth factors

d)

grow slowly

24.

The addition of which of the following ingredients to a culture media would neutralize pH?

a)

salt

b)

heat

c)

sugar

d)

buffers

25.

An organism can synthesize ATP and carbohydrates but can’t make proteins or nucleic acids. Which of the following is missing from the culture medium?

a)

Phosphorus

b)

Nitrogen

c)

CO2

d)

O2

26.

CO2 incubators are used to grow:

a)

capnophiles

b)

aerobes

c)

obligate aerobes

d)

viruses

27.

A medium in which the exact chemical composition varies slightly between batches:

a)

nonselective

b)

differential

c)

complex

d)

simple

28.

In nature, only _____ of microbes are culturable.

a)

39%

b)

3%

c)

1%

d)

12%

29.

Free-swimming bacteria that are not part of a microcommunity are called:

a)

outsiders

b)

mature

c)

planktonic

d)

bacteriums

30.

Why is it that organisms that use and/or tolerate oxygen must have superoxide dismutase?

a)

Oxygen free radicals are toxic

b)

To produce the final electron acceptor superoxide from water

c)

To form metabolic water from oxygen and hydrogen ions

d)

Dismutation requires oxygen

31.

Which of the following conditions do you think would present the greatest problem for bacterial survival?

a)

hypotonic solution

b)

low temperature

c)

neutral pH

d)

hypertonic solution

32.

The normal method of reproduction of bacteria is:

a)

fragmentation

b)

binary fission

c)

transduction

d)

budding

33.

Which of the following would grow slower:

a)

a lab culture of E. coli

b)

the normal microbiota of the colon

34.

Mesophile:

a)

microbes that grow at >80 degrees Celsius

b)

microbes that grow between 20-30 degrees Celsius

c)

microbes that grow between 50-60 degrees Celsius

d)

microbes that grow between -20 & 19 degrees Celsius

e)

microbes that grow between 25-40 degrees Celsius

35.

Thermophile:

a)

microbes that grow at >80 degrees Celsius

b)

microbes that grow between 20-30 degrees Celsius

c)

microbes that grow between 50-60 degrees Celsius

d)

microbes that grow between -20 & 19 degrees Celsius

e)

microbes that grow between 25-40 degrees Celsius

36.

Hyperthermophile:

a)

microbes that grow at >80 degrees Celsius

b)

microbes that grow between 20-30 degrees Celsius

c)

microbes that grow between 50-60 degrees Celsius

d)

microbes that grow between -20 & 19 degrees Celsius

e)

microbes that grow between 25-40 degrees Celsius

37.

Psychrophile:

a)

microbes that grow at >80 degrees Celsius

b)

microbes that grow between 20-30 degrees Celsius

c)

microbes that grow between 50-60 degrees Celsius

d)

microbes that grow between -20 & 19 degrees Celsius

e)

microbes that grow between 25-40 degrees Celsius

38.

Psychrotroph:

a)

microbes that grow at >80 degrees Celsius

b)

microbes that grow between 20-30 degrees Celsius

c)

microbes that grow between 50-60 degrees Celsius

d)

microbes that grow between -20 & 19 degrees Celsius

e)

microbes that grow between 25-40 degrees Celsius

39.

Neutrophile:

a)

microbes that grow best at pH>7

b)

microbes that grow best at pH<7

c)

microbes that grow best at pH=7

40.

Alkaliphile:

a)

microbes that grow best at pH>7

b)

microbes that grow best at pH<7

c)

microbes that grow best at pH=7

41.

Acidophile:

a)

microbes that grow best at pH>7

b)

microbes that grow best at pH<7

c)

microbes that grow best at pH=7

42.

Osmophile:

a)

sugar-loving microbe

b)

salt-loving microbe

c)

methane-loving microbe

43.

Halophile:

a)

sugar-loving microbe

b)

salt-loving microbe

c)

methane-loving microbe

44.

Methanophile:

a)

sugar-loving microbe

b)

salt-loving microbe

c)

methane-loving microbe

45.

The combination of freezing and drying is known as:

a)

desiccation

b)

lyophilization

c)

pasteurization

d)

tyndallization

46.

The time it takes for binary fission to occur is called the

___________________.

a)

lag phase

b)

generation time

c)

stationary phase

d)

woo-hoo phase

47.

The generation time varies from one bacterial species to another.

a)

True

b)

False

48.

In the laboratory under ideal conditions, E. coli has a generation time of about __________ minutes.

a)

5-20

b)

10-20

c)

20-40

d)

100-120

49.

Which phase of binary fission involves normal cell functions besides DNA/cell replication?

a)

r-phase

b)

s-phase

c)

d-phase

d)

i-phase

50.

Which phase of binary fission involves the splitting of one cell into two after z-ring formation?

a)

r-phase

b)

s-phase

c)

d-phase

d)

i-phase

51.

Which phase of binary fission involves DNA replication?

a)

r-phase

b)

s-phase

c)

d-phase

d)

i-phase

52.

Which protein is associated with binary fission?

a)

ftsz

b)

kinase

c)

oxidoreductase

d)

prophase

53.

During which phase of biofilm formation does a planktonic bacterium land on a surface?

a)

maturation 1

b)

maturation 2

c)

irreversible attachment

d)

dispersion

e)

reversible attachment

54.

During which phase of biofilm formation do exopolysaccharides and water channels form?

a)

maturation 1

b)

maturation 2

c)

irreversible attachment

d)

dispersion

e)

reversible attachment

55.
a)

dispersion

b)

irreversible attachment

c)

reversible attachment

d)

maturation 1

e)

maturation 2

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