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WorksheetsLC biology experiments 5th year SOCC review
Total questions: 70
Worksheet time: 37mins
During the course of your studies you examined animal cells under the microscope.
what type of animal cell did you examine?
Human skin cell
Human cheek cell
A bacterial cell
A human white blood cell
What stain did you use to observe the animal cell?
Methylene blue
Iodine
How did you obtain the animal cell?
Licked the slide
Using a scalpel I removed a thin layer of cells
Using a cotton wool bud I swabbed the inside of my mouth
What was added to the slide after the stain?
A drop of water
A piece of tissue
A cover slip
Why is a cover slip added to a microscope slide?
To protect the lens
To preserve the sample
To prevent the sample from drying out
The sample is always observed
Under the highest magnification first
Under the lowest magnification first
If a sample is observed under the x10 magnification lens, the total magnification will be
20
40
100
400
From which plant did you obtain a sample of cells to observe under the microscope?
Geranium
Begonia
Onion
How did you apply the stain to the plant cells?
Used a cotton bud
Used a dropper
Used a mounted needle
How is the cover slip applied to a sample?
Supported by a pencil at 90 degrees and dropped rapidly over the cells
Supported by the finger and thumb and pressed firmly down on the cells
Supported by the finger and thumb then slowly lowered at a 45 degree angle
Why is the cover slip applied in this way?
To hold the cells in place
To prevent air bubbles
To protect the lens
Which diagram represents the structure of the cells you observed under the light microscope?
What type of microscope would allow you to observe the cell ultra structure?
Transmission electron microscope
Hubble microscope
Light microscope
Choose the option with the correct labels
1=eyepiece, 2=nosepiece, 8=light, 12=course focus
9=eyepiece, 2=objective lens, 8=light 12=course focus
9=eyepiece, 2=nosepiece, 8=diaphragm, 12=fine focus
9=eyepiece, 2=nosepiece, 7=diaphragm, 12=course focus
osmosis is a special case of diffusion
True
False
Osmosis is a passive process
True
False
In the experiment to demonstrate osmosis, visking tubing is used as a model for
the cytoplasm
the cell membrane
the cell wall
The control used in the osmosis experiment was
empty visking tubing
visking tubing filled with distilled water in a beaker of sugar solution
visking tubing filled with distilled water in a beaker of distilled water
visking tubing filled with a sugar solution in a beaker of distilled water
a beaker with no visking tubing, filled with distilled water
The results of the osmosis experiment were
I don't know, I wasn't in that day
Both visking tubing did not change at all
The visking tubing with the sugar solution decreased in size and mass
The visking tubing with the distilled water increased in size and mass
The visking tubing with the sugar solution increased in mass and size, while the visking tubing with the water did not change
During the course of your studies, you examined a TS of a divot stem. What does TS stand for?
Total section
Transverse section
Transport section
What is a cotyledon?
A food store found in the stems of plants
A structure used for transport in a plant
A food store found in a seed
A transport structure in a seed
Why did you use a herbaceous stem and not a woody stem?
A woody stem has no vascular bundles
A herbaceous plant is easier to cut through
All woody plants are monocots and a dicot is required for this experiment
The stem was cut using
A knife
A scalpel
A backed blade
Select the correct combination of equipment used in this experiment
Backed blade, paintbrush, clock glass, microscope slide and cover slip, microscope
Scalpel, clock glass, microscope slide and cover slip, mounted needle, microscope
Backed blade, mounted needle, spatula, clock glass, microscope slide and cover slip, microscope
Why were thin sections cut of the stem?
Thin sections are easier to cut
Thin sections allow light to pass through them
taking thin sections means there is more left of the stem for re growth
Which diagram represents what you observed under the microscope?
A
B
Select the correct labels
A=ground tissue, B=xylem C=phloem
A=xylem B=ground tissue C=phloem
A=xylem B=phloem C=ground tissue
A=phloem B=xylem C=ground tissue
During the course of your studies you dissected a sheep’s heart. Why did you wash the heart with cold water before beginning?
To remove bacteria
To remove the smell
To remove blood clots that may obstruct the view of internal structures
How did you distinguish between the left and right side of the heart?
By pinching the sides:the left side is firmer than the right
By locating the coronary artery on the front that runs from the left to right side
All of the above
Where did you make your fist incision, and which tool did you use?
In the left ventricle with a scalpel
In the left atrium with a backed blade
In the right atrium with a scalpel
In the right ventricle with a scalpel
State the location of the bicuspid valve
Between the left atrium and ventricle
Between the right ventricle and atrium
State the location of the entrance to the coronary artery
At the base of the vena cava beside the semi lunar valve
At the base of the aorta beside the semi lunar valve
Select the option with the correct labels below
During the course of your studies you carried out the food tests.
Name the chemical used to test for starch
Biuret Reagent
Benedicts Solution
Sodium hydroxide
iodine
copper sulfate
During the course of your studies you carried out the food tests.
Name the chemical used to test for protein
Biuret Reagent
Benedicts Solution
Sodium hydroxide
iodine
copper sulfate
During the course of your studies you carried out the food tests.
Name the chemical used to test for reducing sugar
Biuret Reagent
Benedicts Solution
Sodium hydroxide
iodine
copper sulfate
Which of the following tests requires heat?
Test for protein
test for starch
test for reducing sugar
test for fat
What colour change is a positive result for the test for protein?
change to lilac
change to brick red
change to blue black
translucent stain
What colour change is a positive result for the test for reducing sugar?
change to lilac
change to brick red
change to blue black
translucent stain
What colour change is a positive result for the test for starch?
change to lilac
change to brick red
change to blue black
translucent stain
For which of the food tests is the initial colour not blue?
starch
protein
fat
reducing sugar
Which of the following is a suitable control for all food tests?
cereal
cordial diluted with water
7 up
water
During the course of your studies you investigated the effect of pH and temperature on enzymes.
What is the chemical nature of an enzyme?
carbohydrate
lipid
protein
A denatured enzyme is
an enzyme that did not form properly
an incomplete enzyme
an enzyme with the wrong sequence of amino acids
an enzyme that has lost its 3D shape and as a result, its function
Where in the cell are enzymes manufactured?
Nucleus
Ribosome
Mitochondria
cytoplasm
Which enzyme did you use to investigate the effect of temperature and pH?
Sucrase
catalase
lipase
From what source did you obtain the enzyme?
Saliva
cooking oil
celery
liver
What was the substrate of this enzyme?
sucrose
hydrogen peroxide
lipid
What was the product of the action of the enzyme on the substrate?
glucose
fatty acids and glycerol
oxygen and water
How did you measure the rate of enzyme activity?
measured the volume of bubbles formed per minute
measured the formation of the black colour per minute
How did you know that enzyme activity had ceased?
There was no colour change
The celery was used up
no more bubbles were produced
the colour change stopped
How did you keep the pH of the reactants constant?
Used a pH meter
added washing up liquid
used a water bath
used pH buffer
How did you keep the temperature of the reactants constant?
Used a pH meter
added washing up liquid
used a water bath
used pH buffer
During the course of your studies you carried out an experiment to denature an enzyme.
How did you denature the enzyme?
Pass the celery through a blender
Boil the celery solution
During the course of your studies you carried out an experiment to immobilise and test an enzyme.
What enzyme did you use?
catalase
sucrase
lipase
What was the source of this enzyme?
celery
liver
yeast
sugar
What was the substrate of this enzyme?
hydrogen peroxide
sucrose
glucose
celery
What is the product of this enzyme?
oxygen and water
fatty acids and glycerol
glucose
What chemicals did you use to immobilise the enzyme?
Sodium alginate and hydrogen peroxide
hydrogen peroxide and calcium chloride
sodium alginate and calcium chloride
After immoblising the enzyme you tested the activity of the immobilsed and free enzyme.
Select the correct set up for this part of the experiment
A
B
How did you test the activity of the enzyme?
Tested for the production of glucose using Clinistix
Tested for the production of fructose using Clinistix
Tested for the production of glucose using Benedicts solution
During the course of your studies you investigated the production of ethanol by fermentation by yeast.
To what kingdom do yeast belong?
Monera
Fungi
Protista
Plantae
Was oxygen needed for this reaction to take place?
Yes
No
How did you ensure anaerobic conditions were present?
Using distilled water
completely dissolving the glucose in water
boiling the water and cooling it
covering the solution with a layer of oil
From what carbohydrate was the ethanol formed?
starch
fructose
glucose
sucrose
How did you maintain the correct temperature for the duration of the experiment?
Left it on the window sill
used a water bath
How did you know the fermentation reaction had completed?
The colour change
All the yeast was gone
No more bubbles of gas were produced
Apart from ethanol, another product was formed during the reaction. How did you test for this product?
It relit a glowing splint
it burned with a pop
it turned limewater milky
To test for the production of ethanol you used potassium iodide and sodium hypochlorite solutions. What did you do before and after adding these solutions?
Filtered then left at room temperature
Decanted then heated in a water bath
Filtered then left overnight at room temperature
Filtered then heated in a water bath
What indicated a positive result for ethanol?
colour changed to blue black
colour changed to brown
no colour change
formation of orange crystals
