WorksheetsB2 Semester Exam 2020
Total questions: 50
Worksheet time: 27mins
Name
Class
Date
1.
Determines the amount and length of activity for a given protein in a test organism
a)
activity assay
b)
PK/PD assay
c)
toxicology assay
d)
stability assay
2.
When multiple samples of known concentrations are measured and graphed so that the concentration of an unknown can be determined is a
a)
standard curve
b)
abnormal curve
c)
ELISA
d)
isoelectric point
3.
Prism-like structure that separates light waves
a)
grating
b)
visible light spectrum
c)
nanometer
d)
a.u.
4.
What organism can naturally produce amylase?
a)
cat
b)
algae
c)
fungi
d)
dog
5.
Which of the following is an indicator used in protein concetration determinations?
a)
ELISA indicator
b)
BPA solution
c)
Bradford reagent
d)
BCIP
6.
What is amylase?
a)
an enzyme
b)
a sugar
c)
a polysaccharide
d)
a starch
7.
Phase of the product pipeline where large volume production of the drug occurs
a)
research and development
b)
manufacturing
c)
clinical trials
8.
Phase of the product pipeline where toxicity and efficacy studies are performed on mice
a)
research and development
b)
manufacturing
c)
clinical trials
9.
Unit of light absorbance
a)
mg/ml
b)
absorbance
c)
a.u.
d)
transmittance
10.
A mass spectrometer may be used for all of the following except?
a)
determing amino acid composition
b)
determining the molecular mass of a compound
c)
determining the chemical analysis of paints dyes, poisons
d)
determining the genetic code of a DNA sample
11.
Which instrument is used for determining the concentration of melanin pigment
a)
UV spec
b)
VIS spec
12.
The pH at which a protein's charge becomes neutral and the protein precipitates out of solution.
a)
amino acid
b)
isoelectric point
c)
density
d)
LJ
13.
What type of experiment is used to test experimental procedures in a limited basis before a full experimental study?
a)
concentration assay
b)
pilot study
c)
western blot
d)
gel electrophoresis
14.
All unauthorized experiments are prohibited.
a)
True
b)
False
15.
A tech sees a 55 kDA band on a PAGE gel and suspects it's amylase. How can she confirm it is?
a)
Eastern blot
b)
Northern blot
c)
Southern blot
d)
Western blot
16.
If the concentation of lactase needed to be measured using Bradford reagent, which instrument would be used?
a)
thermocycler
b)
spectrophotometer
c)
fermenter
d)
capillary detector
17.
Molecules, produced by bacteria or fungus, which inhibit bacterial growth
a)
formulation
b)
transfection
c)
assay
d)
antibiotic
18.
A way to determine how long the pharmaceutical is effective and how it should be stored
a)
activity assay
b)
PK/PD assay
c)
toxicology assay
d)
stability assay
19.
Approved eye protection devices (such as goggles) are worn in the laboratory
a)
to avoid eye strain
b)
to improve your vision
c)
only if you don’t have corrective glasses
d)
any time chemicals, heat or glassware are used
20.
Light with wavelengths between 200-350 nm
a)
mg/ml
b)
grating
c)
spectrophotometer
d)
UV light
21.
The concentration of protein in samples can be estimated by comparing to a
a)
bell curve
b)
standard curve
c)
average curve
d)
milligram curve
22.
A spectrophotometer must be calibrated before every use.
a)
True
b)
False
23.
Which indicator was used to detect amylase because it turns from brown to blue in the presence of starch?
a)
acid
b)
pipet
c)
iodine
d)
casein
24.
Herbal therapies have been used in some cultures for hundreds of years, but caution should be taken for all of these reasons, EXCEPT
a)
they're not subject to FDA approval
b)
adverse effect may occur from the wrong dose
c)
they taste better
d)
certain combos could be harmful
25.
ELISAs are frequently used to assay for proteins during the manufacturing because
a)
they are very general
b)
they are very specific
c)
only occasional samples need to be taken
d)
a simple indicator test is sufficient
26.
A test for determining the presence or a particular characteristic of a protein
a)
formulation
b)
transfection
c)
assay
d)
antibiotic
27.
Helps researchers measure the amount of protein present
a)
concetration assay
b)
PK/PD assay
c)
toxicology assay
d)
stability assay
28.
Flammable materials, like alcohol, should never be dispensed or used near
a)
an open door
b)
an open flame
c)
another student
d)
a sink
29.
The absorbance of known concentrations of protein on a standard curve allows the determination of?
a)
protein size
b)
protein shape
c)
protein concentration
d)
protein activity
30.
Which instrument can recognize the molecular weight of a compound?
a)
UV spec
b)
VIS spec
c)
mass spec
d)
IR spec
31.
An instrument that shines light and measures the amount of light transmitted through a sample
a)
mg/ml
b)
grating
c)
spectrophotometer
d)
transmittance
32.
Unit of protein concentration
a)
mg/ml
b)
grating
c)
spectrophotometer
d)
transmittance
33.
Which instrument is used for checking for the presence of an unknown colorless protein
a)
UV spec
b)
VIS spec
34.
Instead of multi-tasking, humans generally take turns completing tasks, which is called
a)
multi-tasking
b)
procrastination
c)
vibe checking
d)
switch tasking
35.
Horseplay or practical jokes in the laboratory are
a)
okay if you are working alone
b)
not dangerous
c)
okay
d)
always against the rules
36.
What is the most convenient way to determine the absorbance spectrum of a single sample?
a)
UV/Vis spec
b)
Balance
c)
pH paper
d)
pH meter
37.
The wavelengths of light that the human eye can see
a)
mg/ml
b)
visible light spectrum
c)
spectrophotometer
d)
transmittance
38.
Used to determine what are safe and unsafe levels of a given drug
a)
concetration assay
b)
ELISA
c)
toxicology assay
d)
stability assay
39.
If a lab experiment is not completed, you should
a)
make up some results
b)
come in during lunch and finish while eating lunch
c)
sneak in after school and work alone
d)
discuss the issue with your instructor
40.
Never leave a lit burner unattended.
a)
True
b)
False
41.
Chipped or cracked glassware is okay to use.
a)
True
b)
False
42.
If a laboratory fire erupts, immediately
a)
notify your instructor
b)
run for the fire extinguisher
c)
throw water on the fire
d)
open the windows
43.
Recombinant DNA
a)
is used to produce 100's of other DNA products
b)
can be found in fungus cells
c)
is produced by pasting DNA from 2 sources together
d)
is larger than a typical chromosome
44.
Phase of the product pipeline where safety and efficacy studies on human subjects are monitored by the FDA
a)
research and development
b)
manufacturing
c)
clinical trials
45.
The following footwear is best in the laboratory.
a)
shoes appropriate for the weather
b)
open-toed shoes
c)
closed-toed shoes
d)
sandals
46.
If you do not understand a direction or part of a lab procedure, you should
a)
skip it and go on to the next part.
b)
ask the instructor before proceeding
c)
try several methods until something works
d)
figure it out as you do the lab
47.
The amount of transmittance is related to the amount of absorbance by?
a)
T/A equation
b)
transmittance assays
c)
absorbance indication
d)
Beer's law
48.
Amount of light that passes through a sample
a)
UV light
b)
visible light spectrum
c)
transmittance
d)
a.u.
49.
The wavelength at which a sample absorbs the most light
a)
absorbance
b)
Lamba(max)
c)
nanometer
d)
a.u.
50.
You are heating a piece of glass and now want to pick it up. You should
a)
pour cold water on it
b)
use tongs
c)
pick up the end that looks cooler
d)
use a rag or paper towels
100 %
