WorksheetsBiochemistry full course
Total questions: 100
Worksheet time: 55mins
Which of the following are the 4 essential elements that make up 96% of living matter?
H, O, N, P
C, O, N, H
C, O, S, H
O, S, P, N
Which macromolecules contain nitrogen? (Always True, not Sometimes)
Carbohydrates
Lipids
Nucleic Acids
Proteins
Which macromolecule contains phosphorus? (Always true, not Sometimes true)
Carbohydrates
Lipids
Nucleic Acids
Proteins
What is the main purpose of carbohydrates in your body?
energy storage
insulation/fat storage
as catalysts
quick energy
To separate a mixture of salt and water what separation method is used?
Filtration
Distillation
Chromatography
Evaporation to dryness
Which of the following techniques can we use to separate homogeneous mixtures?
Crystallisation
Distillation
Evaporation
All of the above
In preparation for an electrophoresis procedure, enzymes are added to DNA in order to
convert the DNA into gel
cut the DNA into fragments
change the color of the DNA
produce longer sections of DNA
In preparation for an electrophoresis procedure, enzymes are added to DNA in order to
convert the DNA into gel
cut the DNA into fragments
change the color of the DNA
produce longer sections of DNA
For which of the following would it NOT be appropriate to use electrophoresis as part of analysis?
Separating proteins from a cell
DNA fingerprinting
Separation of enzymes based on their activity
Paternity test
What is the function of sodium dodecyl sulfate in electrophoresis?
to track the passage of the sample
a detergent that will denature proteins
a reducing agent
to increase the density of the sample
What is the function of glycerol in electrophoresis?
to track the passage of the sample
a detergent that will denature proteins
a reducing agent
to increase the density of the sample density
What is ethidium bromide used for during electrophoresis?
used to denature proteins
used as a tracking gel
used to visualised DNA fragments in uv light
Select correct name for the amino acid shown
Ala
Cys
Trp
His
Select correct names for the amino acids shown
Lys and Leu
Met and Trp
Ala and Val
Asp and Glu
Select correct name for the amino acid shown
Gly
Ala
Leu
Val
Which of the following amino acid is NOT shown in the picture above?
Asp
Asn
Arg
Gln
Select correct name for the amino acid shown
Glu
Gln
Asn
Arg
Which of the following is the name of a side chain of an amino acid shown above?
N-butyl amine
Imidazole
Guanidino
Indole
Select correct name for the amino acid shown
Pro
Trp
Tyr
Thr
Select correct name for the amino acid shown
Ala
Asp
Asn
Arg
Which one of the following amino acids is shown in the picture above?
Ala
Asp
Leu
Lys
Which of the following best explains why enzymes are necessary for reactions in our cells?
The reactions are too slow to meet the needs of the cell if enzymes are missing.
Enzymes supply the oxygen necessary for the reactions.
Enzymes change reactants from solid to liquid during the reactions.
The reactions take up too much space in the cell if enzymes are missing.
The ____________________ mechanism describes how each substrate must fit into the enzyme.
lock and key
hammer and nail
jigsaw puzzle
seesaw
The _____________________ mechanism describes how an enzyme changes shape once the substrate is bonded.
hammer and nail
induced fit
jigsaw puzzle
lock and key
___________________ can bind to the binding site A and inhibit the enzymatic activity.
Competitive inhibitor
Allosteric regulator
Non-Competitive inhibitor
Cofactor
What is cofactor?
A non-protein portion that is required for proper functioning of an enzyme.
A protein that is required for proper functioning of an enzyme.
A non-protein portion that decreases the rate of reaction of the enzyme.
A protein that decreases the rate of reaction of the enzyme.
Most coenzymes may be included in classes of foodstuffs known as:
Minerals
Fats
Vitamins
Metallic ions
Which is the correct comparison about coenzyme and prosthetic group?
Both are organic cofactor.
Both are inorganic cofactor.
Coenzyme is organic cofactor while prosthetic groups is inorganic cofactor.
Prosthetic group binds loosely to enzyme while coenzyme binds tightly at the active sites of enzyme.
What impedes enzymatic reactions by binding to the enzyme and causes conformational changes of the active site?
non-competitive inhibitor
competitive inhibitor
coenzymes
cofactors
Organic molecules that temporarily bind to enzymes and enhance an enzyme's ability are called:
Cofactors
Coenzymes
Allosteric Inhibitors
Allosteric Activators
Competitive and non-competitive inhibitors always _______ the enzymatic reaction rates.
decreases
increases
stabilize
cancel
A diet lacking in ___________ leads to the development of a disease known as scurvy.
Vitamin A
Vitamin B
Vitamin C
Vitamin D
Vitamin D's major function:
To prevent birth defects in unborn children
To keep the bones supplies with Calcium
To help the red blood cells absorb iron
Calcium helps to:
Prevent birth defects
Vision
Build strong bones
Which foods are the best source of Vitamin C:
Fresh fruits and vegetables
Cooked fruits and vegetables
Animal foods
Which vitamins are fat soluble:
B complex and C
Iron and Calcium
A,D,E,K
What does Vitamin A do?
It helps your metabolism.
It helps you see.
Helps your genes.
Helps your brain.
What does Vitamin D do?
Helps you see.
Absorbs calcium from food.
Helps you walk.
Helps your brain.
Deficiency of __________ causes Night blindness.
Vitamin B1
Vitamin D
Vitamin A
Having foods rich in this vitamin will prevent Beriberi disease.
Vitamin A
Vitamin C
Vitamin B1
Infection and bleeding of gums can be prevented by having foods rich in ___________.
Iron
Calcium
Vitamin C
Which foods are NOT rich in lipids?
Fats
Oils
Pasta
Butter
Which of the following is NOT a function of a carbohydrate?
quick, accessible energy for workouts
major structural component of biological membranes
structural rigidity in plant cell walls
structural rigidity in insect exoskeletons
Which one of the following is a function of lipids?
storing genetic information in all life
long term energy storage in animals
rigid structural component of fungi cell walls
speeding up chemical reations
Many carbohydrate names end with what suffix: (try and remember the sugars you have heard of)
ase
ese
in
ose
This type of fat (often found in plants) is liquid at room temperature because the crinkly nature of its carbon chains cause them to pack together less densely.
monosaccharide
polysaccharide
saturated fat
unsaturated fat
Which carbohydrate/function pair is NOT correct?
cellulose/rigid structural component of fungi cell wall
chitin/rigid structure component in exoskeletons
glycogen/accessible energy in muscles and liver
starch/storeable energy in plants
Which of the following molecular features would best help you identify a protein by its structure?
rings filled with N and a P
one or two Ns attached to C
a chain of C surrounded by H almost exclusively
a chain or ring of C surrounded by alternating H and OH
Which of the following molecular features would best help you identify a lipid by its structure?
rings filled with N and a P
one or two Ns attached to C
a chain of C surrounded by H almost exclusively
a chain or ring of C surrounded by alternating H and OH
Which function(s) listed below do nucleic acids provide for living things?
short-term energy storage
long-term energy storage
store genetic information
structural support
speed up chemical reactions
Chromatography is used to separate the components of a mixture and can be carried out in a range of different ways.
a) A suitable example of a ‘carrier gas’ in gas chromatography is
Chlorine
Nitrogen
Steam
Not possible to state, as there should be a vacuum
(b) Separation is achieved in gas chromatography due to the components in the mixture having different
Interactions with the stationary phase.
Interactions with the mobile phase.
Colours
Solubility in the moving solvent.
Which gas is the least suitable as a carrier gas in Gas-Liquid Chromatography?
Argon
Carbon Dioxide
Oxygen
Nitrogen
Chromatography may be used to separate mixtures of amino acids. This is best explained by the fact that different amino acids have different interactions with
the mobile phase only
the stationary phase only
the mobile phase and the stationary phase
ninhydrin
In one type of HPLC, the stationary phase is non-polar and a polar solvent is used as the eluent. Which of the following would travel through the chromatography column most quickly?
Tetrachloromethane
Chloromethane
Iodomethane
Hexane
In HPLC (High Performance Liquid Chromatography) which of these factors does not affect the time taken for a component to pass through the column?
Type of detector
Material of stationary phase
Particle size of stationary phase
Temperature of column
In gas chromatography, the time taken for a substance to travel through the column is called
Reference time
Retention time
Separation time
Travelling time
Which of the following columns are not used in HPLC
Analytical column
Separation column
Guard column
Capillary column
Which of the following will improve the efficiency of separation process in liquid chromatography?
Increase in sample size, increase in column diameter
Reduction in sample size, increase in column diameter
Increase in sample size, reduction in column diameter
Reduction in sample size, reduction in column diameter
What type of structural lipid is the major component of membranes
triglycerides
glycerophospholipids
sphingolipids
sterols
An unsaturated fatty acid is typically connect the which carbon on a glycerophospholipid
carbon 1
carbon 2
carbon 3
Which is not a common head group for phospholipids
Serine
Choline
galactose
ethanolamine
Which of the following is NOT a type of sphingolipid?
phosphatidylcholine
sphingomyelin
ganglioside
ceramide
............................. contains alternating residues of D-glucuronic acid and N-acetylglucosamine.
Pectine
Blood Group Antigens
Chondroitin sulfate
Hyaluronic Acid
Heparan sulfate
Under acidic conditions, lipid molecule hydrolysis produces
Glycerol + Water
Glycerol + Fatty acids
Glycerol + Fatty acid salts
Water + Fatty acids
Which is incorrectly paired?
fats - energy storage lipids
steroid hormones - messenger lipids
glycerophospholipids - membrane lipids
cholesterol - protective coating lipids
The ff are unsaturated fatty acids, EXCEPT
Arachidonic acid
Palmitoleic acid
Docosahexanoic acid
Arachidic acid
This lipid makes up 95% of stored lipids in the body.
Triglycerides
Monosacharrides
Cholesterol
Phospholipids
The presence of which element in nucleic acids distinguishes them from proteins?
nitrogen
carbon
sulfur
phosphorus
Triglyceride is composed of:
Glycerol, 2 fatty acids
3 Glycerols and 1 fatty acids
3 Glycerides
Glycerol, 3 fatty acids
