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Biokimia II

Total questions: 50

Worksheet time: 37mins

Name
Class
Date
1.

DNA merupakan penyimpan utama material genetik sebagai sandi untuk menyampaikan Informasi yang diperlukan. Setiap Nukleotida terdiri atas....

a)

Basa Nitrogen Purin atau Pirimidin, Gula pentosa, dan Phosfat group

b)

Basa Nitrogen Purin atau Pirimidin, Gula Deoksiribosa, dan Phosfat group

c)

Basa Nitrogen Purin, Pirimidin dan Gula pentosa

2.

Basa Purine dan pyrimide yang terikat kepada gula deoxyribose, dapat berputar bebas. DNA membentuk pasangan basa yang spesifik A-T memiliki 3 ikatan hidrogen, G-C memiliki 2 ikatan Hidrogen

a)

Benar

b)

Salah

3.

Informasi yang salah tentang perbedaan antara DNA dan RNA adalah...

a)

Pada DNA basa Purin terdapa Adenin dan Guanin, sedangkan pada RNA memiliki basa Sitosin dan Urasil

b)

Gula pada DNA merupakan gula Ribosa, sedangkan pada RNA merupakan deoksiribosa

c)

Enzim penghidrolisis pada DNA adalah DNase, sedangkan pada RNA terdapat RNase.

4.

Daerah gene yang tidak dirubah menjadi mature mRNA dan tidak menjadi protein adalah...

a)

Exon

b)

Intron

c)

Codon

d)

Domain

5.

Enzim yang berperan untuk memutuskan ikatan phosphodiester pada DNA adalah..

a)

DNA Helicase

b)

Isomerase

c)

Topoisomerase

d)

DNA Polimerase

6.

Enzim yang berperan untuk membuka double strands DNA adalah..

a)

DNA Helicase

b)

Isomerase

c)

Topoisomerase

d)

DNA Polimerase

7.

Enzim yang berperan untuk menstabilkan single strand DNA setelah dibuka adalah...

a)

DNA Polimerase

b)

SSB

c)

SBS

d)

RNA Polimerase

8.

Daerah fragmen pada satu strand yang disintesis secara continously merupakan...

a)

Lagging Strand

b)

Okazaki Fragments

c)

Leading Strand

9.

tRNA dimodifikasi terlebih dahulu sebelum keluar dari Nukleus, dibantu oleh

a)

RNA Polimerase II

b)

RNA Polimerase III

c)

Aplha-Factor

d)

Aplha-Factor 2

10.

Asam amino sebelum dibawa keribosom terlebih dahulu diaktivitasi oleh....

a)

aminoacyl-tRNA synthetase

b)

RNA Polimerase III

c)

Hydrolytic editing

d)

ATP

11.

Sub unit pada Ribosom yang merupakan tempat pengikatan tRNA-Peptidil adalah..

a)

A

b)

P

c)

E

12.

................... merupakan bagian tRNA yang penting dalam proses translasi (non-anticodon) dan mutasi pada bagian ini bisa menyebabkan disease

a)

D Loop

b)

Acceptor Stem

c)

T Loop

d)

Identity Element

13.

Sub unit kecil pada Ribosom yang merupakan tempat pengikatan tRNA– Aminoasil

a)

A

b)

E

c)

P

14.

Which sequence of DNA bases would pair with this partiaal strand ATG TGA CAG

a)

ATG TGA CAG

b)

GTA AGT GAC

c)

TAC ACT GTC

d)

CAT TCA CTG

15.

Which of the following units are repeatedly joined together to form a strand of DNA?

a)

amino acids

b)

nucleotides

c)

polysaccharides

d)

fatty acids

16.

Why is DNA important?

a)

it is very small and very complicated

b)

it serves as the blueprint for traits of all living things

c)

it's in everything

d)

because we eat it every day for energy

17.

The DNA sequence ATCAGCGCTGGC is part of a gene. How many amino acids are coded for by this message?

a)

4

b)

6

c)

8

d)

10

18.

DNA sequence is ACAGTGC. How would this be coded on mRNA?

a)

UGUCACG

b)

CACUGUA

c)

TGTCACG

d)

GUGACAU

19.

Where does translation take place?

a)

Nucleus

b)

Ribosome

c)

Golgi Body

d)

Cytoplasm

20.

An enzyme speeds up a reaction by

a)

lowering the activation energy.

b)

raising the activation energy.

c)

absorbing energy.

d)

releasing energy.

21.

What type of macromolecules does an enzyme belong?

a)

Protein

b)

Lipid

c)

Nucleic Acid

d)

Carbohydrate

22.

Dalam Siklus Transkripsi RNA Polimerase Bakteri, terdapat 7 tahapan siklus. Tahapan............. merupakan proses pembukaan rantai DNA.

a)

1

b)

2

c)

3

d)

4

23.

Berikut ini yang bukan merupakan karakteristik dari Promoter Sequences adalah...

a)

Satu gen Satu Promotor

b)

Promoter memiliki dua hexameric Sekuens DNA, -35 dan -10

c)

Weak promoter menghasilkan gene yang melimpah

d)

Strong promoter menghasilkan gene yang melimpah

24.

.................... merupakan enzim tanpa adanya bagian non-protein dan belum aktif

a)

Active Site

b)

Apoenzyme

c)

Holoenzyme

d)

Cofactor

25.

Berikut ini yang bukan termasuk dalam Cofactor adalah....

a)

Biotin

b)

Gugus Fungsi

c)

Mineral

d)

Pepsin

26.

Tipe enzim yang bekerja berdasarkan jenis ikatan tertentu disebut........

a)

Tipe Absolut

b)

Tipe Group

c)

Tipe Lingkage

d)

Urease

27.

Berikut ini yang termasuk kelompok enzim tipe Group adalah...

a)

Urease

b)

Lipase

c)

Chymotrypsin

d)

Hexokinase

28.

What is the role of Michaelis-Menten equation?

a)

To calculate the free energy

b)

Mathematically explain the relationship of enzyme's velocity and the substrate concentration

c)

Mathematically explain the saturation of enzyme in substrate

29.

What is Vmax ?


(you can choice more than one answer)

a)

With a fix concentration of enzyme, the formation of product will not increase even if you add more substrate

b)

Related to a situation where the Michaelis-Menten plot reaches a plateau ('flat')

c)

A maximum value for Velocity

d)

Maximum substrate that is been converted to product

30.

Apa itu nilai Km?


(Pilihlah lebih dari satu jawaban)

a)

The unit for length is cm. The unit for Km is substrate concentration

b)

Km is the value of [S] when the velocity is half Vmax

c)

Km is Michaelis-Menten constant

d)

Km is kilometer, how far the enzyme can move

31.

In which type of Inhibition inhibitor binds with ES complex

a)

Uncompetitive

b)

Competitive

c)

Non competitive

d)

All of the above

32.

In which type of inhibition inhibitor has the tendency to bind with both E and ES complex

a)

Competitive

b)

Non competitive

c)

Un competitive

d)

Mixed

33.

Which is Michaelis Menten equation

a)

V= Vmax S/(Km+S)

b)

1/V= 1/Vmax+Km/Vmax S

c)

V= Vmax S/2(Km+S)

d)

V=Vmax-KmV/S

34.

Which type of inhibition has structural resembles of I and E

a)

Non competitive

b)

Competitive

c)

Uncompetitive

d)

Non of the above

35.

In to how many groups enzymes are classified as

a)

Four

b)

Five

c)

Six

d)

Eight

36.

Tight binding interaction of enzyme substrate indicates ......... Km value

a)

Large

b)

Initially large and then small

c)

Initially small and the large

d)

Small

37.

Yang merupakan kelas Enzyme Hydrolase adalah....

a)

Oksidase

b)

Reduktase

c)

Kinase

d)

Amylases

38.

What are enzymes??


(Pilih sebanyak-banyaknya)

a)

Catalyst

b)

Protein Moiety only

c)

Most enzymes are globular proteins

d)

Increase reaction rates without being used up

e)

Enzyme can be used therapeutically

39.

Enzymes as Biological Catalysts......

a)

Enzymes are proteins that increase the rate of reaction by lowering the energy of activation

b)

They catalyze nearly all the chemical reactions taking place in the cells of the body.

c)

Not altered or consumed during reaction

d)

Non-Reusable

40.

What is the central dogma?

a)

DNA to RNA to Amino Acid

b)

DNA to RNA to Protein

c)

RNA to DNA to Amino Acid

d)

RNA to DNA to Protein

41.

Ceklislah beberapa jenis RNA...

a)

mRNA

b)

pRNA

c)

cRNA

d)

tRNA

e)

rRNA

42.

What is produced by transcription?

a)

mRNA

b)

tRNA

c)

rRNA

d)

DNA

43.

What is a codon?

a)

A set of 3 nucleotides on mRNA

b)

A whole strand of mRNA

c)

A set of 3 nucleotides on DNA

d)

An amino acid

44.

What type of RNA is responsible for bringing amino acids?

a)

mRNA

b)

tRNA

c)

rRNA

d)

DNA

45.

Where does Transcription take place?

a)

Inside the nucleus

b)

Inside the Ribosome

c)

Inside the cytoplasm

d)

Inside the Rough ER

46.

Which enzyme creates the new mRNA polyer by adding the complementary free RNA nucleotides?

a)

RNA polymerase

b)

Exonuclease

c)

DNA polymerase

d)

DNA Helicase

47.

What salt is used salt fractionation in Enzyme Precipication?

a)

Aluminum sulphate

b)

Sodium chloride

c)

Ammonium Sulphate

d)

Sodium sulphate

48.

This is the chief principle of Dialysis

a)

Capillary action

b)

Osmosis

c)

Adhesion

d)

Cohesion

49.

What is homogenization

a)

Removing the cell debris

b)

Making a homogenous mixture of sample and buffer

c)

Centrifugating the samples

d)

None of the above

50.

Bagian tRNA yang berperan dalam mengikat asam amino (Amino acid attachment) adalah....

a)

1

b)

2

c)

3

d)

4