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WorksheetsMicrobiology
Total questions: 121
Worksheet time: 2hrs 1mins
It is prerequisite for effective disinfection
Cleaning
Disinfection
Sterilization
Antisepsis
Reduce the number of micro-organisms to a level at which they are not harmful
Cleaning
Disinfection
Sterilization
Antisepsis
Disinfection of living tissue- an application of an agent which inhibits the growth of microorganisms
disinfection
Antisepsis
Bacteriostatic agent
Bactericide agent
Sporicide agent
Inhibits the growth of bacteria but doesn’t necessarily kill them
Antisepsis
Bacteriostatic agent
Bactericide agent
sporicide agent
Kills bacteria-most don’t kill endospores
Antisepsis
Bacteriostatic agent
Bactericide agent
Sporicide agent
Kills spores
antisepsis
Bacteriostatic agent
Bactericide agent
Sporicide agent
What the doctor should do before put a respiratory equipment on a patient ?
Cleaning & drying
Cleaning followed by disinfection
Cleaning followed by sterilization
Time required to kill 90% of micro-organisms
D value
Z value
Temperature change at D value required to reduce the micro-organisms population
D value
Z value
Temperature below 100c
Boiling
Autoclave
Pasteurisation
Temperature above 100c
Boiling
Inspissator
Autoclave
Hot water bath
Used to sterilize media
inspissator
Hot water bath
Boiling
Steaming
Kills microorganisms by coagulating their proteins
Dry heat
Moist heat
Kills by oxidation effects and denaturing proteins
Dry heat
Moist heat
To inactivate non sporing bacteria for preparation of vaccines also for sterilization of serum
Inspissator
Hot water bath
Boiling
Steaming
Kills vegetative forms of bacterial pathogens - kills hiv and mycobacteria - doesn’t kill spores
these are characteristics of ?
Inspissator
Hot water bath
Boiling
Steaming
For media containing sugar and gelatin
Boiling
Hot water bath
Steaming
Tyndallisation/ intermittent sterilization
Sterilisation is carried out under pressure at 121c for 15 min
Inspissator
steaming
Intermittent sterilization
Autoclave
Advantage of autoclave
Penetrating power
Non toxic
Fast method
All above
the lab physician applied Bowie dick tape to the article being autoclaved, pale brown stripes appear across the tape which indicates
Process has been satisfactory
Poor heat penetration
Technique which using geobacillus SPORES in it
Mechanical technique
Physical technique
Chemical technique
Biological technique
considered as hot air sterilisation
Non-ionising
Ionising
Ultraviolet rayes and infrared rayes are ..... , x-rays and gamma rayes are .....
Ionizing, Non-ionizing
Non-ionizing, ionizing
Both are ionizing
Both are Non-ionizing
It’s bactericidal, sporicidal and it kills spores
Aldehyde
Phenol
Halogens
Alcohol
destroying anthrax spores in hair and wool
cidex
Glutaraldehyde
Formaldehyde
For postoperative skin disinfection
tincture of iodine
Iodophores
Chlorine
Mercurials
used as disinfectant in swimming pool
Iodine
Chlorine
Mercurial
Copper salts
Marked bacteriostatic
Halogens
Dyes
Heavy metal ions
phenol
Used as droplets in the eyes of newborn to protect against neisseria gonorrhoea
Silver nitrate
Mercurials
Copper salts
Acridine dyes
is Active against viruses ........,
used as fungicide .........
Mercurials, copper salts
Silver nitrate, silver sulphadiazine
Acridine dyes, aniline dyes
Ethidium bromide, iodine
Used to prevent infection of burn tissues
Silver sulphadiazine
copper salts
Mercuric chloride
Analine dyes
Ethidium bromide
bactericidal because the interaction with nucleic acids and used as antiseptic to treat mild burns
Acridine dyes
Aniline dyes
Ethidium bromide
Active against gram positive bacteria and has no activity against tubercle bacilli
Acridine dyes
Aniline dyes
Ethidium bromide
Bacteriostatic, intercalated between base pairs of DNA, form a fluorescent complex with DNA
ALL these characteristics belongs to
Acriflavine
Aniline dyes
Ethidium bromide
Mercuric chloride
Not active against Gram-negative bacilli
Hydrogen peroxide
Surfactant
Ethylene oxide (EO) gas
Formaldehyde gas
Decomposes in light and broken down by catalase
Hydrogen peroxide
Formaldehyde gas
Ethylene oxide
Ozone gas
Used for furmigation of operation theatre
Hydrogen peroxide
Formaldehyde gas
Ethylene oxide
Ozone gas
Used for heat sensitive instruments, the materials must be well aerated after sterilization , it’s mutagenic and carcinogenic
Ethylene oxide
Ozone gas
Hydrogen peroxide
surfactant
Aearation is necessary in ..........
Ethylene oxide
Hydrogen peroxide
Ozone gas
All above
Aeration is NOT necessary in
Hydrogen peroxide-ozone gas
Ozone gas only
Formaldehyde-hydrogen peroxide
All
Destroy natural gum rubber (latex)
Ozone gas
Hydrogen peroxide
Ethylene oxide
Disposable-carcinogenic
Candle filter
Asbestos filter
Sintered glass filter
Membrane filter
If we want to prepare a solution for parenteral use , we should use
Candle filter
Asbestos filter
Sintered glass filter
Membrane filter
medium incubation
10-12 days
7-10 days
13-17 days
15-20 days
Transmitted by Airborne
Influenza v
Chicken pox
Rubella
Cytomegalovirus
Virus has very long incubation period with no symptoms.........,
virus remains completely latent with periodic reactivation...........,
Virus can be continuously detected: mild or no clinical symptoms ..........,
Chronic infection, latency, slow V infection
slow V infection, chronic infection, latency
Latency, slow v infection , chronic
Slow v infection , latency, chronic
Mechanism of Cell damage and death can be arranged as
Shut off-competition-integration-mutation-inflammation -host immune response
Shut off-integration-mutation-inflammation-competition -host immune response
Shut off-competition-mutation- integration-inflammation -host immune response
None
Playing the major role in clearing virus infection ..........,
protect against reinfection...........
Humoral immunity, cellular immunity
Cellular immunity, humoral immunity
None
Peak 7-10 days post infection and resolve ongoing virus infections
IFN-NK-ADCC
CTL
Antiviral ab
One of them causing a reduced response to an antigen, may be
due to genetic factors, pre-natal infection, molecular mimicry
Immune tolerance
Down-regulation of MHC class I
Infection of immuno-sequestered sites
Antigenic variation
Aggregates of stainable substances and usually consist of viral capsid proteins
interfero
Inclusion bodies
both
after we did a gram stain for a patient it appears that he have a gram-positive red & spore forming bacteria, which of the following it could be
Listeria
Bacillus
Corynebacterium
Lactobacillus
A small Gram-positive bacteria that doesn’t have spores and it looks like Chinese letters
Cornyebacteria
Clostridum
Bacillus
Listeria
The Elek’s test shows positive to toxigenicity to which bacteria?
Clostridium
Bacillus
Cornyebacteria
Lactobacillus
A patient had fever and muscle pain and the gram staining shows bacteria with motility and tumbling movement
Listeria monocytogenes
Lactobacillus
Clostridium
Propionibacterium
we did a gram staining and it shows that the bacteria is anaerobic which of the following it could be
B.anthracis
B.cereus
Lactobacillus
Cl.tetani
Which of the following clostridium caused gangrene
Cl.tetani
Cl.botulinum
Cl.perfringens
Cl.difficile
Cause diphtheroids
Cornyebacteria
Bacillus
Cl.tetani
Listeria
Normal flora of female genital tract
Listeria monocytogenes
Lactobacillus
Listeria
A female, 18 years old ate a poorly cooked meats then she had a fever and diarrhea which of the following could be the responsible bacteria?
Listeria monocytogenes
Cl.tetani
Cl.difficle
propionibaterium
Which of the following is True about Listeria monocytogenes
It’s a gram-Positive cocci
Gram-negative bacilli
Cause acne
Can tolerate in cold temperature
Capsulated in vivo and forms spores in vitro also stained with acid fast spore stain
Bacillus
Clostridium
Listeria
Cornyebacteria
Tetaospasmin produced by
Cl.tetani
Cl.difficile
Cl.botulinum
Cl.perfringens
If there’s an infection of the umbilical mostly caused by
B.anthracis
Cl.tetani
B.cereus
Infant botulism
Sub-terminal endospores - performed toxin in the food and the toxin acts on voluntary and autonomic nervous system
Cl.tetani
Cl.difficile
Cl.perfringens
Cl.botulinum
Feeding of honey to the baby it’s a possible cause of
Infant botulism
Neonatal tetanus
B.cereus
B.anthracis
We use Lecithinase test and stormy clot formation to diagnose it
Cl.perfringens
Cl.botulinum
Cl.titani
Cl.difficile
Associated with broad-spectrum antibiotic therapy, causing pseudomembranous colitis, its spores are major source of nasocomial outbreaks
Cl.titani
Cl.perfringens
Cl.difficile
Cl.botulinum
A sample with gram + cocci and catalase (-) and beta hemolytic on blood agar also it’s bacitracin sensitive
S.pneumoniae
S.aureus
S.epidermidis
S.pyogens
Sample with gram + cocci bacteria and alpha hemolytic also sensitive to optochin test
S.pneumonie
S.aureus
S.pyogenes
Listeria
A gram + cocci, catalase + , with Negative coagulase test
S.epidermidis
S.aureus
S.pyogens
S.pneumonie
Protein A found in ?
S.aureus
S.pyogens
S.pneumonie
Protein M found in?
S.pyogens
S.aureus
S.epidermidis
S.pneumonie
One of the following is Catalase function
Cleaves DNA&RNA
Degradation of H2o2 to O2
All above
Cause urinary tract infection in sexually active young women
S.saprophyticus
S.aureus
S.epidermidis
S.pyogens
It’s a disease characterized by sore throat and an erythematous rash
Necrotizing fasciitis
Scarlet fever
Toxic shock syndrome
Follicular tonsillitis
Patient with sore throat and as complication he had arthritis and carditis, the reason could be
Acute rheumatic fever (ARF)
Acute glomerulonephritis
Scarlet fever
Cellulitis
Gram+ cocci, encapsulated, diplococci .. All these characteristics belongs to
S.pyogens
S.viridans
S.agalactiae
S.pneumonie
non-hemolytic, normal inhibitant of intestine , nosocomial infection, classified as group D streptococci
Enterococci
S.pneumonia
S.viridans
S.epidermidis
Which of the following wants to be sterile from micro organisms
Skin
Large intestine
Mouth
Blood
Which of the following considers pathogens in normal flora of respiratory tract
Streptococcus aureus
S.neisseria
S.epidermidis
Heamophilus influenza
Which of the following consider the most common cause of HAI
Parasites
Fungi
Bacteria
Virus
Which of the following is the major cause of HAI
CRE
MRSA
VRE
Which of the following not usually used to prevent transmission of micro organisms spread by direct contact
Gloves
Hand washing
Mask
Gown
When Placing a urinary catheter we should do
Sterile technique
Antiseptic technique
Clean technique
The doctor wants to take a blood sample from the patient, he should take it
In early morning
During fever
Both
The (best) site of CSF puncture
L4-L5
L2-L3
L3-L4
L5-S1
Clean catch Is the preferred method in
Urine sample
Cervical swab
Stool sample
Sputum
Used mainly for collection of urine from infants
Catheterization
Mid steam clean catch
Suprapubic aspiration
Which bacteria produces vitamins B12 and K
E.coli
Neisseria
Brucellosis
40% of Neonatal meningitis caused by
E.coli
B.anthracis
Neisseria meningitidis
Which of the following cause pediatric diarrhea
Enteropathogenic E.coli
Enteroinvasive E.coli
Enteroaggregative E.coli
Enterohaemorragic E.coli
Causes dysentery which is similar to shigellosis
Enteropathogenic E.coli
Enteroinvasive E.coli
Enteroaggregative
Enterohaemorragic
Cause infantile diarrhea
Enterohaemorragic
Enteroinvasive
Enteroaggregative
Enteropathogenic
O157:H7 is the most common strain of
EHEC
EAggEC
EIEC
EPEC
Produce verotoxin
EHEC
EAggEC
EPEC
EIEC
Non-lactose fermenting colonies that cause typhoid and septicaemia
Salmonella
E.coli
Klebsiella spp
B.anthracis
Oxidase negative, catalase positive
reduce nitrate to nitrite
Enterobacteriaceae
E.coli
Salmonella
Vibrio
A female 19 years old, went to the hospital because she had watery diarrhea with no blood, the doctor suspected that the cause was
ETEC
EPEC
EIEC
EHCH
Wich of the following tests used to detect typhoid fever
Widal test
PCR
Elek’s test
Which of the following Cause The 4F’s disease
Salmonella
Shigella
E.coli
klebsiella
The doctor looks at the agarand found swarming ureas positive non-lactose fermenter and very pleomorphic which could be
Proteus
Pseudomonas
Shigella
Klebsiella
Characteristic odour, aerobic bacteria, positive oxidase, produce exopigment (pyocyanin) and it’s a common cause of nosocomial infections
Pseudomonas aeruginoss
B.anthracis
Shigella
Proteus
Comma shaped, single flagellum, does a surface pellicle on alkaline peptone and cause a rise water stool
Vibrio Cholera
Klebsiella
Shigella
Proteus
Helical shape,lophtrichous, associated with gastritis and cancers also produce VacA and CagA
Helicobacter pylori
Yersinia
Heamophilus influenza
Bordetella pertussis
Pathogen of animals, cause bubonic plague and pneumonic plague
heamophilus influenza
Yersinia
Shigella
Helicobacteria
How to do prophylaxis to cornyebacteria and titanus and bordetella?
Tab vaccine
Hib vaccine
DPT vaccine
Ty21a vaccine
They function as facultative intracellular parasites
Yersinia
Neisseria
Brucellosis
Heamophilus influenza
Thayer Martin agar for the growth of which of the following
brucellosis
Neisseria
Bordetella pertussis
Yersinia
A lab physician took a look on a bacteria under microscope, he saw that it contains LOS and Opa which of the following could be this bacteria
Neisseria gonorrhoea
Neisseria meningitidis
Brucellosis
Bordetella pertussis
Ratio between toxic does antibiotic does, or ratio of LD50 to ED50
Selective toxicity
therapeutic index
Broad spectrum
Affect a variety of bacteria both gram-positive and gram-negative
Selective toxicity
Therapeutic index
Broad spectrum
Which of the following block the synthesis of peptidoglycan
Penicillin
Vancomycin
Cephalosporins
All above
Which of the following is an antibiotic that works on B-lactam
Teicoplanin
Vancomycin
Pinicillin
Bacitracin
it’s the last step of peptidoglycan synthesis and used against penicillin-resistance stains
Cephalosporins
Penicillin
vancomycin
Teicoplanin
Inhibition of cell wall synthesis by inhibiting the early step of peptidoglycan synthesis
Bacitracin
Vancomycin
B-lactamase
Cephalosporins
Bind to 30S ribosomal subunit
Aminoglycoside
All
Chloramphenicol
Macrolides
Gentamycin, streptomycin are
Bactericidal,tetracycline
Bacteriostatic,tetracycline
Bacteriostatic,aminoglycosides
Bactericidal,Aminoglycosides
1-Rifampicin, 2-Quinolones and novobiocin, 3- Sulfonamides and Trimethoprim
1-Inhibit RNA synthesis, 2-Inhibit DNA synthesis, 3- inhibit folic acid synthesis
1-Inhibit DNA synthesis, 2-inhibit folic acid synthesis, 3-inhibit RNA synthesis
1- inhibit folic acid synthesis, 2-Inhibit DNA synthesis, 3-Inhibit RNA synthesis,
If we add penicillin with tetracycline the result will be
Indifference
Addition
Synergism
Antagonism
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