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WorksheetsBiotech review
Total questions: 68
Worksheet time: 56mins
What is involved in creating genetically modified bacteria?
allowing them to reproduce freely
changing their food source
using biotechnology techniques
growing them on selected plants
Biotechnology uses a procedure called genetic engineering. Which best describes genetic engineering?
making artificial DNA in the lab from chemicals
cutting out pieces of DNA from an organism under a microscope
adding DNA from one organism into the DNA of another organism
What type of science allows us to alter the DNA of a corn plant so that it will contain more protein?
biology
biotechnology
geology
physiology
Bacteria use restriction enzymes to _____.
destroy foreign protein
synthesize DNA
synthesize RNA
destroy foreign DNA
synthesize protein
When a typical restriction enzyme cuts a DNA molecule, the cuts are staggered so that the DNA fragments have single-stranded ends. This is important in recombinant DNA work because ______.
the fragments will bond to other fragments with complementary single-stranded ends
the single-stranded ends serve as starting points for DNA replication
only single-stranded DNA segments can code for proteins,
it enables researchers to use the fragments as introns
it allows a cell to recognize fragments produced by the enzyme,
In recombinant DNA technology, the term vector refers to ______.
the enzyme that cuts DNA into restriction fragments
a heat-resistant DNA polymerase
a plasmid or other agent used to transfer DNA into a living cell
a DNA probe used to locate a particular gene
the sticky ends of a DNA fragment
Genetic profiles are used to determine whether Sam could be the father of Becky's baby. Sam is not the father if __________ genetic profile shows some bands not present in __________ genetic profile.
the baby's ... Becky's
Sam's ... the baby's
the baby's ... Sam's or Becky's
Becky's ... the baby's
the baby's ... Sam's
Which of the following would be considered a transgenic organism?
a bacterium that has been treated with a compound that affects the expression of many of its genes
a human treated with insulin produced by E. coli bacteria
a fern grown in cell culture from a single fern root cell
a rat with rabbit hemoglobin genes
All of the listed responses are correct.
What does a spectrophotometer measure?
color change
abs of light
mass of solute
volume of a solution
Convert 3mL to uL
3000 uL
300 uL
0.003 uL
0.03 uL
Which pipet should you use to measure 7mL of water?
green
blue
p1000
p20
What is the range of a p1000?
2-20uL
20-200uL
200-1000uL
200-2000uL
Biotechnology is the manipulation of living organisms or their components to produce useful, usually commercial products.
True
False
What is the name of the process by which DNA is taken up from the environment?
transduction
translation
transformation
conjugation
What is the name of the process by which bacteria shuffle DNA between one another?
transformation
conjugation
translation
transduction
Which is the process by which DNA is transferred from one bacteria to another by a virus?
transduction
conjugation
transformation
What is the name for a sequence of DNA that allows RNA polymerase to bind so that transcription can occur?
ori
selection marker
gene of interest
promoter
What is the name of the part of the plasmid that carries the gene under study?
ori
promoter
gene of interest
selection marker
What is the name for the part of the plasmid used to select for successful transformants?
ori
selection gene
gene of interest
multiple cloning site
What is the name of the plasmid part where replication starts?
ori
selection gene
gene of interest
promoter
What are the two main uses of plasmids?
genetic modification
analyzing proteins
protein production
growing bacteria
What is a multiple cloning site?
a place where lots of genes are inserted in a plasmid
a company that has many locations where it clones things
a short DNA sequence that contains many restriction sites
a place where many sheep are cloned
What is a recombinant bacterium?
One that contains non-native DNA (DNA from another source)
One that has swapped its genetic information with another organism
any bacteria that has a plasmid
a bacteria that has changed the order of the genes in its chromosome
What is the main focus of biochemisty?
proteins and their function
genes and their function
connecting genes to the proteins they make
What is the main focus of genetics?
proteins and their function
genes and their function
connecting genes to the proteins they make
What is the main focus of molecular biology?
proteins and their function
genes and their function
connecting genes to the proteins they make
What is the name of the process that uses DNA as a template to create RNA?
(a)
What holds DNA strands together?
(a)
What is the name of the nitrogenous base that pairs up with adenine?
adenine, A
thymine, T
guanine, G
cytosine, C
What is the name of the nitrogenous base that pairs up with guanine?
adenine, A
thymine, T
guanine, G
cytosine, C
What is the name of the nitrogenous base that pairs up with thymine?
adenine, A
thymine, T
guanine, G
cytosine, C
What is the name of the nitrogenous base that pairs up with cytosine?
adenine, A
thymine, T
guanine, G
cytosine, C
Which part of a nucleotide is the start of a DNA molecule?
phosphate group
sugar
phosphate
hydrogen bond
What is meant by "antiparallel"?
The two strands arecomplimentary
The strands run in opposite directions
A always pairs with T and G always pairs with C
All strands are double helices
On which strand can DNA be replicated continuously?
complementary strand
lagging strand
replication fork
leading strand
What is transcription?
The process by which DNA replicates itself
The process by which DNA is used as a template for RNA
The process by which DNA strands bind together
The process by which RNA is used as a template for protein expression
What type of genome does a retrovirus have?
DNA
RNA
protein
carbohydrate
What domain of life is the most abundant form and diverse of life on Earth?
Archa
Eukarya
Plantae
Bacteria
Why are model organisms used in science?
because they are simplified systems that can be easily manipulated
because they show us exactly how diseases work in all organisms
because they be mad cute like models, y'all
What microorganism was first used as a model system to understand diseases?
Influenza b
Malaria
Yersenia pestis
Anthrax
Who are the two men known as the founders of microbiology?
Koh
Pasteur
Mendel
Anthrax
What is the difference between Gram-positive and Gram-negative bacteria?
One is only present in pathogenic bacteria
Gram positive has thick layers of peptidoglycan
Gram negative has thick layers of peptidoglycan
One tastes better on s'mores than the other
What type of bacteria is pictured?
bacillus
coccus
spirillum
virus
What type of bacteria is pictured?
bacillus
coccus
spirillum
virus
What type of bacteria is pictured?
bacillus
coccus
spirillum
virus
Nucleus
Which organelle belongs in animal cells only?
Chloroplast
Cell Wall
Many Small Vacuole
Nucleus
Animal Cell
Plant Cell
A tool that measures degrees is called ____.
ruler
scale
thermometer
What measures volume of a liquid?
ruler
scale
graduated cylinder
An instrument that uses a lens to make tiny things look big is a ________.
microscope
compass
beaker
What does this tool protect?
Goggles protect the eyes.
Googles protect clothing.
Goggles protect your hands.
Which science tool(s) would you use that involve milliliters (mL)?
beaker and graduated cylinder
beaker and ruler
graduated cylinder and meter stick
triple beam balance
