WorksheetsMCB100 Module 2
Total questions: 72
Worksheet time: 59mins
contains membrane-bound organelles such as a nucleus in which the welldefined chromosomes (bodies containing the hereditary material) are located, mitochondria, and an endoplasmic reticulum.
Eukaryotic Cell
Prokaryotic Cell
Their DNA is found in the cell’s nucleus, which is separated from the cytoplasm by a nuclear membrane, and the DNA is found in multiple chromosomes.
Eukaryotic Cell
Prokaryotic Cell
Their DNA is consistently associated with chromosomal proteins called histones and with non-histones.
Eukaryotic Cell
Prokaryotic Cell
Their cell walls, when present, are chemically simple. Cell division usually involves mitosis, in which chromosomes replicate and an identical set is distributed into each of two nuclei.
Eukaryotic Cell
Prokaryotic Cell
include protozoa, fungi, plants, and animals. These organisms are grouped into the biological domain Eukarya.
Eukaryotic Cell
Prokaryotic Cell
unicellular organisms that lack organelles or other internal membrane-bound structures.
Eukaryotic Cell
Prokaryotic Cell
does not have a true nucleus or membrane-bound organelles.
Eukaryotic Cell
Prokaryotic Cell
Their DNA is not enclosed within a membrane and is usually a singular circularly arranged chromosome
Eukaryotic Cell
Prokaryotic Cell
Prokaryotes do not have a nucleus, but, instead, generally have a single chromosome: a piece of circular, double-stranded DNA located in an area of the cell called the (a) .
Prokaryotes, their cell walls almost always contain the
polysaccharide (a) .
Prokaryotes usually divide by (a) .
Organisms within the domains Bacteria and Archaea
are based on the ___ cell.
Eukaryotic Cell
Prokaryotic Cell
(a) are collective of one or more types of microorganisms that can grow on many different surfaces.
A biofilm is defined as a community of micro-organisms irreversibly attached to a surface and encased in an (a) .
(a) are usually round but can be oval, elongated, or flattened on one side.
Cocci that remain in pairs after dividing are called (a)
Cocci that divide and remain attached in chainlike patterns are called (a) .
Cocci that divide in two planes and remain in groups of four are known as (a) .
Cocci that divide in three planes and remain attached in cube-like groups of eight are called (a) .
Cocci that divide in multiple planes and form grapelike clusters or broad sheets are called (a) .
(a) divide only across their short axis
Most bacilli appear as single rods, called (a) .
Bacilli that apear in pairs after division.
(a)
Bacilli that occur in chains.
(a)
Bacilli that are oval and look so much like cocci that they are called (a) .
Bacteria that look like curved rods are called (a) .
(a) , have a helical shape, like a corkscrew, and rigid bodies.
Another group of spirals are helical and flexible; they are called (a) .
the genus (a) ; and triangular cells.
they maintain a single shape
(a)
they can have many shapes
(a)
is a viscous (sticky), gelatinous polymer that is external to the cell wall and composed of polysaccharide, polypeptide, or both. Its chemical composition varies widely with the species.
(a)
If the substance is organized and is firmly attached to the cell wall, the glycocalyx is described as a (a) .
If the substance is unorganized and only loosely attached to the cell wall, the glycocalyx is described as a (a) .
are long filamentous appendages that propel bacteria.
(a)
Flagella may be distributed over the entire cell.
(a)
Flagella may be at one or both poles or ends of the cell.
(a)
Flagella may be a single flagellum at one pole
(a)
A tuft of flagella coming from one pole.
(a)
flagella at both poles of the cell.
(a)
___ bacteria have a lipopolysaccharide-lipoprotein phospholipid outer membrane surrounding a thin peptidoglycan layer.
Gram-negative
Gram-positive
In gram stain, it combines with peptidoglycan.
crystal violet–iodine complex
decolorizer
In gram stain, it removes the lipid outer membrane of gram-negative bacteria and washes out the crystal violet.
crystal violet–iodine complex
decolorizer
(a) is a bacterial genus that naturally lacks cell walls.
Archaea have (a) ; they lack peptidoglycan.
Acid-fast cell walls have a layer of (a) outside a thin peptidoglycan layer.
In the presence of lysozyme, gram-positive cell walls are destroyed, and the remaining cellular contents are referred to as a (a) .
In the presence of lysozyme, gram-negative cell walls are not completely destroyed, and the remaining cellular contents are referred to as a (a) .
(a) are gram-positive or gram-negative bacteria that do not make a cell wall.
(a) , irregular infoldings of the plasma membrane, are artifacts, not true cell structures.
Plasma membranes can be destroyed by (a) .
In (a) , energy is expended to modify chemicals and transport them across the membrane.
The cytoplasm of a prokaryote contains numerous ___ ribosomes; ribosomes consist of rRNA and protein.
70S
80S
60S
50S
reserve deposits found in prokaryotic and eukaryotic cells.
(a)
(a) are resting structures formed by some bacteria; they allow survival during adverse environmental conditions.
The process of endospore formation is called ___.
sporulation
germination
The process of endospore formation is called ___.
sporulation
germination
the return of an endospore to its vegetative state is called ___.
sporulation
germination
Cell morphology tells us little about phylogenetic relationships. However, morphological characteristics are still useful in identifying bacteria (example: differences in such structures as endospores or flagella can be helpful).
Morphological Characterization
Differential Staining
Biochemical Tests
Microscopic examination of a Gram stain or an acid-fast stain is used to obtain information quickly in the clinical environment.
Morphological Characterization
Differential Staining
Biochemical Tests
Enzymatic activities are widely used to differentiate bacteria.
Morphological Characterization
Differential Staining
Biochemical Tests
Such tools are designed to perform several biochemical tests simultaneously and can identify bacteria within 4 to 24 hours.
(a)
is the science that studies serum and immune responses that are evident in serum.
Serological Testing
ELISA
Western Blot
A reaction between the known antibodies and the bacteria provides identification of the bacteria.
Serological Testing
ELISA
Western Blot
Is a laboratory method used to detect specific protein molecules from among a mixture of proteins. This mixture can include all the proteins associated with a particular tissue or cell type.
Serological Testing
ELISA
Western Blot
is the identification of bacterial species and strains by determining their susceptibility to various phages.
Phage typing
Flow cytometry
DNA fingerprints
NAATs (nucleic acid amplification tests)
nucleic acid hybridization.
measures physical and chemical characteristics of cells.
Phage typing
Flow cytometry
DNA fingerprints
NAATs (nucleic acid amplification tests)
nucleic acid hybridization.
The number and sizes of DNA fragments, produced by restriction enzymes are used to determine genetic similarities.
Phage typing
Flow cytometry
DNA fingerprints
NAATs (nucleic acid amplification tests)
nucleic acid hybridization.
can be used to amplify a small amount of microbial DNA in a sample. The presence or identification of an organism is indicated by amplified DNA.
Phage typing
Flow cytometry
DNA fingerprints
NAATs (nucleic acid amplification tests)
nucleic acid hybridization.
Single strands of DNA, or of DNA and RNA, from related organisms will hydrogen-bond to form a double-stranded molecule; this bonding is called ___.
Phage typing
Flow cytometry
DNA fingerprints
NAATs (nucleic acid amplification tests)
nucleic acid hybridization.
DNA chips, and FISH are examples of nucleic acid hybridization techniques.
Southern blotting
Dichotomous keys
are used for the identification of organisms. Cladograms show phylogenetic relationships among organisms.
Southern blotting
Dichotomous keys
