WorksheetsThe Quizziz That I'm not Familiar With
Total questions: 75
Worksheet time: 38mins
If the chromogen used for ALP assay is PNPP, the yellow product is due to:
p-nitropyruvate
p-nitrophthalate
p-nitrophenol
p-nitrophosphate
p-nitrophrenolate
Elevated blood levels of ammonia occur in all of the following disorders
EXCEPT:
hepatic cirrhosis
diabetes mellitus
Reye’s syndrome
renal failure
Which reagent would not dissolve indirect bilirubin?
caffeine-benzoate
cetrimide
methanoic acid
dimethylsulfoxide (DMSO)
A breakdown product of hemoglobin is:
Bilirubin
Bence Jones protein
Hematoxylin
Lipoprotein
The old name for ALT is:
sGPT
sGOT
sGNT
sGTT
Mesobilirubinogen, urobilinogen and stercobilinogen are collectively known
as:
mesobilirubins
urobilins
bilirubin products
urobilinogen
Which of the following organs/tissues produce AST?
heart
RBCs
kidneys
liver
skeletal muscles
Which hepatic enzymes are generally HIGHER among obese people?
ALP
GGT
ALT
AST
All of these
Post-hepatic jaundice may be due to:
Viral hepatitis
Crigler-Najjar syndrome
Rotor syndrome
Gilbert syndrome
Which reagent would not dissolve indirect bilirubin?
dimethylsulfoxide (DMSO)
methanoic acid
cetrimide
caffeine-benzoate
It is one of the best tests for bilirubin:
Jendrassik – Grof
Isaacson – Jensey
Reitman and Frankel
Biuret
The specific protein for the transport of copper present in plasma is:
Ceruloplasmin
Transferrin
Immunoglobulin
Albumin
Increased levels of indirect bilirubin only is seen in:
Acute quartan malaria
Pancreatic tumor
Bile duct obstruction
Gallstones
The indicator enzyme in sGOT assay be found in the _____________ cycle.
tricarbonylate cycle
tricarbonate cycle
tricarotinic cycle
tricarboxylate cycle
If the total bilibirubin is 3.1 mg/dL and the conjugated bilirubin is 2.0 mg/dL.,
unconjugated bilirubin is
2.2 mg/dL
1.1 mg/dL
0.5 mg/dL
5.1 mg/dL
Type I glycogen storage disease is due to a deficiency in glucose-6-
phoshatase in the liver. This condition is called:
Pompe’s disease
Hers disease
Gaucher’s disease
von Gierke’s disease
If the chromogen used for ALP assay is PNPP, what wavelength is used in the
absorbance measurement?
410 nm
630 nm
380 nm
555 nm
455 nm
The coenzyme required in reactions involving transaminases is derived from:
pyridoxine
riboflavin
thiamine
biotin
niacin
Which of the following is not a function of the liver?
production of hippurate and bile
production of bile acids
25-hydroxylation of vitamin D
storage of iron
Choose among these hepatic enzymes that belong to Class 1 enzymes.
ALP only
5'-NT
OCT
LDH
Which hepatic enzymes are generally HIGHER in men than women?
ALT
GGT
ALP
AST
All of these
Which of the following serum constituents is greatly decreased if a blood
specimen is left standing at room temperature for 8 hours before processing?
Glucose
Carbon dioxide
TAG
Bilirubin
Oxidation of urobilinogen by microorganisms will produce:
porphobilinogen
urobilin
stercobilinogen
protoporphyrin
Which of the following enzyme pairs cannot be used in the diagnosis of liver 1/1
disorders?
ACP & ALS
LDH & AST
GGT & 5’-NT
ALP & LAP
Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) are
both elevated in which of the following disease?
urobilinogen
diazobilirubin
viral hepatitis
muscular dystrophy
A critically ill patient becomes comatose. The physician believes the coma is 1/1
due to hepatic failure. The assay most helpful in this diagnosis is:
AST
GCT
Ammonia
ALT
Which hepatic enzymes is UNAFFECTED by race?
ALT
GGT
AST
ALP
The sensitive enzymatic indicator for intravascular hemolysis and acute
myocardial infarction is:
alanine aminotransferase (ALT)
Gamma-glutamyl transferase (GGT)
alkaline phosphatase (ALP)
aspartate aminotransferase (AST)
Which of the following enzymes are used in the diagnosis of pancreatic
cancer?
Aspartate aminotransferase (AST) and leucine aminopeptidase (LAP)
5’-nucleotidase (5’-NT) and gamma-glutamyl transferase (GGT)
Amylase (AMS) and lipase (LPS)
alanine aminotransferase (ALT) and lactate dehydrogenase (LD)
Which enzyme ratio is the best indicator of acute or chronic hepatitis:
LD1 /LD5 ratio
AST/ALT
CK-MB/total CK ratio
Amylase/lipase ratio
The most widely used methods for bilirubin measurement are those based on 1/1
the :
Jaffe reaction
8-hydroxyquinoline reation
Jendrassik Grof method
Schales and Schales method
Type I glycogen storage disease is due to a deficiency in glucose-6-
phoshatase in the liver. This condition is called:
Pompe’s disease
Gaucher’s diseaseGaucher’s disease
von Gierke’s disease
Hers disease
One of the following is used to assess liver function. Which one is it?
Hippuric Acid Test
Para-aminohippurate test
Inulin Clearance test
Creatinine Clearance Tes
Which of the following tests has NO clinical application in assessing liver
function?
Gamma-glutamyl transferase
Triglyceride
Amylase
Lactate dehydrogenase isoenzymes
Which hepatic enzymes are AFFECTED by dietary intake?
AST
ALT
GGT
ALP
Choose the enzymes used to diagnose hepatobiliary diseases.
AST only
ALT only
AST & ALT
ALP & ACP
5'-NT
A serum sample was assayed for bilirubin at 10AM and the result was 12
mg/dL. The same sample was retested at 3PM. The result now is 8 mg/dL/. The
most likely explanation for this discrepancy is:
The reagent has deteriorated
A calculation error in the first assay
The sample was exposed to light
The sample was not refrigerated
Which hepatic enzyme has DIURNAL VARIATION?
AST
ALT
GGT
ALP
The transport material associated with bilirubin in the liver is?
ligandin
alpha 1 -globulin
albumin
beta-globulin
All of the following methods have been used for the measurement of of
serum bilirubin EXCEPT:
Bilirubin oxidase
excretion
Bilirubinometer BSP
Jendrassik-Grof
The polarity and water solubility of bile acids are increased upon conjugated
with:
Either glycine or taurine
Neither glycine not taurine
Taurine
Glycine
Acetaminophen is particularly toxic to the:
Heart
Kidney
Liver
Spleen
The indicator enzyme in sGPT assay be found in the _____________ pathway.
glyoxylic
glycolytic
glycoxylic
glycogenolytic
Which of the following enzymes are used in the diagnosis of acute hepatitis?
5’-nucleotidase (5’N) and gamma-glutamyl transferase (GGT)
Aspartate aminotransferase (AST) and creatine kinase (CK)
Amylase (AMS) and lipase (LPS)
Aspartate aminotransferase (AST) and alanine aminotransferase (ALT)
Reduction of bilirubin occurs in the:
Small intestine
Spleen
Liver
Colon
In the Jendrassik – Grof method for the determination of serum bilirubin
concentration, quantitation is obtained by measuring the green color of:
urobilinogen
bilirubin glucuronide
diazobilirubin
azobilirubin
What enzyme system is responsible in the oxidation of bilirubin?
leucine aminopeptidase
glucose-6-phosphate dehydrogenase
carbamoyl phosphate synthetase
bacterial oxidases
What substance gives feces its normal color:
Stercobilin
Urobilin
Uroerythrin
Urochrome
The most heat labile fraction of alkaline phosphatase is obtained from:
Bone
Placenta
Intestine
Liver
In which of the following disease states is unconjugated bilirubin NOT a majo
serum component?
Neonatal jaundice
Erythroblastosis fetalis
Gallstones
HDN
In bilirubin determinations, the purpose of adding a concentrated caffeine
solution or methyl alcohol is to:
Allow mixing of B1 and B2
Measure total bilirubin
Precipitate protein
Dissolve conjugated bilirubin
Indirect-reacting bilirubin may be quantified by reacting it initially in which
reagent?
dilute HCl
dilute sulfuric acid
caffeine-benzoate
sodium hydroxide
In the bilirubin oxidase method, bilirubin is oxidized to a colorless compound 1/1
which is:
diazotized
bilierythrin
sulfanilic acid
urobilinogen
biliverdin
Which hepatic enzymes are AFFECTED by physical exertion?
AST
ALT
ALP
GGT
Which of the following functions as a transport protein for bilirubin in the
blood?
alpha1 -globulin
albumin
beta-globulin
gamma-globulin
The greatest activities of serum AST and ALT are seen in:
Acute viral hepatitis
Alcoholic cirrhosis
Metastatic pancreatic carcinoma
Primary biliary cirrhosis
What coenzyme is required in reactions involving transaminases?
PTP
PPP
PLP
PSP
Which method uses sulfanilic acid, HCl and sodium nitrite?
Zimmerman
Diazo
Jaffe
Lowry
Which of these is NOT a liver function test:
Lipid profile
Thymol turbidity test
TP A/G ratio & BCG test
hemoglobin electrophoresis
Which fraction is expected to be elevated in alcoholic cirrhosis of the liver?
ALP
GGT
ACP
LDH
Choose the enzymes used to diagnose parenchymal liver disorders.
ALP & ACP
AST but not ALT
AST & ALT
ALT but not AST
Formation of bile acids is the major mechanism for eliminating:
Triglycerides
Cholesterol
Bilirubin
Phospholipids
Which two physiological conditions are characterized by elevated serum
alkaline phosphatase?
rickets, hyperthyroidism
viral hepatitis, myocardial infarction
growth, third trimester of pregnancy
obstructive jaundice, biliary cirrhosis
What class of compounds are used as substrate for ALP assays?
monophosphatase
paraphosphates
polyphosphates
pyrophosphates
orthophosphates
ALP & LAP concentrations are useful to assess:
Intestinal malabsorption
Kidney function
Diabetes mellitus
Hepatobiliary disease
What is the immediate precursor of biliverdin?
urobilinogen
bilirubin
heme
senescent erythrocytes
Total lactic dehydrogenase (LD) activity, confirmed to fraction 4 and 5 , is
most likely to be associated with:
Hemolytic anemia
Myocardial infarction
Acute viral hepatitis
Pulmonary infarction
All of the following describe the direct bilirubin EXCEPT:
excreted in jaundiced patients’ urine
conjugated in the liver
conjugated with glucuronic acid
insoluble in water
Which hepatic enzymes are usually HIGHER in children than ADULTS?
ALP
GGT
ALT
AST
If the total bilirubin is 4.3 mg/dL and the conjugated bilirubin is 3.1 mg/dL, the
unconjugated bilirubin is:
34.20 umol/L
58.1 4 umol/L
37.62 umol/L
20.52 umol/L
The principle of the tablet test for bilirubin in urine or feces is:
chemical coupling of bile with a diazonium salt to form a brown color
the liberation of oxygen by bile to oxidize orthotolidine to a blue-purple color
chemical coupling of bilirubin with a diazonium salt to form a purple color
the reaction between bile and 2,4- dichloronitrobenzene to a yellow color
Kernicterus is an abnormal accumulation of bilirubin in:
Brain tissue
Liver tissue
Kidney tissue
Heart tissue
What organ produce the enzyme ALT ?
brain
lungs
liver
heart
muscles
The term delta bilirubin refers to:
Water-soluble bilirubin
Direct-reacting bilirubin
Free unconjugated bilirubin
Bilirubin tightly bound to albumin
Increased total bilirubin due to increased direct bilirubin suggests:
obstructive jaundice
hemolytic jaundice
neonatal jaundice
Crigler-Najjar syndrome
