microbe that can only grow when oxygen isn't present

(a)  

microbe that can only grow when oxygen is present

(a)  

microbe that can grow in oxygen but doesn't require oxygen for energy metabolism

(a)  

microbe that's flexible and can grow with or without oxygen

(a)  

bacteria with optimal growth between -5 and 15°C

(a)  

bacteria with optimal growth between 45 and 75°C

(a)  

bacteria with optimal growth between 25 and 45°C

(a)  

bacteria with optimal growth between 25 and 45°C

(a)  

a bacterium that grows at a pH of 10+

(a)  

a bacterium that grows at a pH of 4

(a)  

a bacterium that grows at a pH of 7

(a)  

these microbes can grow in excessive sugar concentrations

(a)  

these microbes require a high concentration of sodium chloride in order to grow (i.e. Halobacterium salinarium)

(a)  

these microbes can grow in moderate concentration of sodium chloride

(a)  

a cell that has more solutes than the surrounding environment is

(a)  

a cell with fewer solutes than the surrounding environment is

(a)  

water flows (into/out of) a cell that's hypotonic

(a)  

water flows (into/out of) a cell that's hypertonic

(a)  

when the temperature increases past the optimal, the hydrogen bonding in the RNA breaks down and the proteins denature in this structure

(a)  

when the temperature increases past the optimal, lipids can be destroyed (freeze and fracture) affecting which structure?

(a)  

What temperature results in white colonies of Serratia?

(a)  

when the temperature decreases past the optimal, the activity of this molecule will slow down

(a)  

when the temperature increases past the optimal, these denature

(a)  

what temperature results in pinkish/red colonies of Serratia?

(a)  

higher absorbance = (more/less) cell growth

(a)  

refers to the concentration of hydrogen ions

(a)  

a cell with the same solute concentration as its environment

(a)  

compound that kills/inhibits any type of microorganisms

(a)  

compound that kills/inhibits bacteria

(a)  

compound that kills/inhibits viruses

(a)  

Penicillin targets what?

(a)  

Ampicillin targets what?

(a)  

Bacitracin targets what?

(a)  

Tetracycline targets what?

(a)  

Streptomycin targets what?

(a)  

Chloramphenicol targets what?

(a)  

substance that inhibits/kills microorganisms but is gentle enough to apply to living tissue

(a)  

chemical agents applied to inanimate objects to kill microbes

(a)  

compound that kills/inhibits fungi

(a)  

compound that kills/inhibits animals

(a)  

Which antibiotics target the DNA and RNA synthesis of bacteria?

(a)  

Which antibiotics target the protein synthesis of bacteria?

(a)  

A substance that has been oxidized has (a)   an electron

Fermentation is a form of energy metabolism where the final electron acceptor is (a)   .

rxn that breaks down a molecule using water

(a)  

test that examines protein hydrolysis

(a)  

test that examines the deamination of an amino acid

(a)  

The API 20 E tests for

(a)  

The API 20 E tests for bacteria that do

(a)  

Which test examines aerobic/anaerobic respiration?

(a)  

Which tests examine protein utilization?

(a)  

Which tests examine carbon utilization?

(a)  

anaerobic respiration is a form of energy metabolism where the final electron acceptor is

(a)  

aerobic respiration is a form of energy metabolism where the final electron acceptor is

(a)  

What molecule is hydrolyzed by proteases?

(a)  

What molecule is hydrolyzed by amylase?

(a)  

What molecule is hydrolyzed by lipase?

(a)  

What molecule is deaminated into phenylpyruvic acid and ammonia?

(a)  

What molecule is degraded in indole, ammonia, and pyruvic acid?

(a)  

What molecule splits into carbon dioxide and ammonia when it's hydrolyzed?

(a)  

mixed acid fermentation test uses what reagent to indicate a positive rxn?

(a)  

What enzyme is tested for with hydrogen peroxide?

(a)  

the 2,3 butanediol fermentation test uses what reagent to indicate a positive rxn?

(a)  

what enzyme is tested for in an oxidation test?

(a)  

name a test that must be done under anaerobic conditions in the API20E

(a)  

The generation of cellular energy, ATP, can have metabolic rxns that require a particular amount of which physical property?

(a)  

When using a fluid thioglycollate media which type of bacteria would be present at the bottom of the media?

(a)  

Name a test that examines nitrogen utilization in the API20E

(a)  

What's the lowest taxonomic level that 16s rRNA sequence analysis is useful for identification?

(a)  

In DNA extraction, the 1st step is to...

(a)  

In DNA extraction, the 2remove lipids and proteinsnd step is to...

(a)  

In DNA extraction, the 3rd step is to...

(a)  

What PCR reagent indicates to the Taq polymerase which part of the DNA should be replicated (amplified)?

(a)  

What PCR reagent provides the nucleotides/basepairs that are used by the Taq polymerase to make copies of the DNA target?

(a)  

Which phase of PCR raises the temperature to break the DNA into single strands?

(a)  

Which phase of PCR allows for the primers to form hydrogen bonds with the single stranded DNA?

(a)  

Which phase of PCR uses the Taq polymerase to add complementary nucleotides to the single strand of DNA?

(a)  

When identifying a bacteria using BLAST, what metric is used?

(a)  

the most widely used molecular tool in biology

(a)  

the most widely used bioinformatic tool in biology

(a)  

genetic locus typically used to identify bacteria and archae

(a)  

2 genetic loci typically used to identify fungi and other eukaryotes

(a)  

PCR reagent that stabilizes the molecular rxns

(a)  

PCR reagent that creates covalent bonds to make the new DNA strand

(a)  

PCR reagent that hybridizes with the DNA at the location where amplification will occur

(a)  

PCR reagent that is the building blocks of the new DNA

(a)  

type of PCR used to quantify how much RNA is being produced

(a)  

type of PCR used to amplify a large amount of a DNA target and then sequenced

(a)  

type of PCR used as a diagnostic to distinguish between different microbes

(a)  

PCR phase with an optimal temperature of 72°C

(a)  

PCR phase with an optimal temperature of 95°C

denaturation

(a)  

PCR phase with an optimal temperature between 50 and 60°C

(a)  

BLAST result used as a comparison between searches

(a)  

BLAST result illustrating how much of the inputted sequence is used in the alignment

(a)  

BLAST result that's the number of alignments that occur by chance

(a)  

BLAST result illustrating how well the inputted sequence matches to sequences in the database

(a)  

The E. coli used in lab is either resistant to streptomycin or ampicillin. Which type of plasmid is responsible for this trait?

(a)  

The streptomycin resistance gene is located on

(a)  

The ampicillin resistance gene is located on

(a)  

The nalidixic acid resistance gene is located on

(a)  

What resistance gene is transferred during conjugation?

(a)  

Mutations create variation in which microbial organisms?

(a)  

horizontal gene transfer creates variation in which microbial organisms?

(a)  

crossing over and independent assortment create variation in which microbial organisms?

(a)  

mutation that changes the DNA and changes the protein by shortening or truncating it

(a)  

mutation that changes the DNA but doesn't change the protein

(a)