WorksheetsHistopathology- Staining 2- Cytopathology 2
Total questions: 63
Worksheet time: 32mins
What is the use of reticulin stain in histology?
to demonstrate - glycogen
to demonstrate - reticular fibres and basement membrane
to identify acid- fast organisms
all of the above
In reticulin staining, reticulin fibers are stained gives which color to the structure in histology?
red color
blue/ black color
green color
orange color
The detection of ferric iron in tissues, blood smears , or bone marrow smears can be done by using which stain?
reticulin stain
iron stain
PAP stain
H & E stain
What is the clinical application of iron stain in histology?
Hemochromatosis
Liver histopathology
Hemosiderosis
Both A & B
Both A & C
What is the composition of phosphotungstic acid & hematoxylin (PTAH) stain?
phosphotungstic acid with eosin
hematoxylin with phosphotungstic acid
phosphotungstic acid with iron staining
phosphotungstic acid with reticulin stain
After staining the sample with phosphotungstic acid hematoxylin (PTAH) stain, the phosphotungstic acid binds all of the available hematin to form which type of pigment?
white lake pigment
black color in all follicle
blue lake pigment
none
Which specific staining technique, stains the muscle cross striations , fibrin , nuclei and other tissue elements blue?
iron stain
reticulin stain
phosphotungstic acid hematoxylin (PTAH)
PAS stain
What are the clinical application of phosphotungstic acid hematoxylin (PTAH) stain?
contractions bands, as seen in contraction band necrosis
demonstrating striated muscle fibers and mitochondria
tumors of skeletal muscle
gliosis in the central nervous system
All of the above
Fibrin deposits in lesion can be seen by which staining technique in histology?
phosphotungstic acid hematoxylin (PTAH) stain
PAS stain
Reticulin stain
Iron stain
What is the other name of acid fast stain in histology?
reticulin stain
calcium stain
Ziehl Neelsen stain
PAS stain
What all reagents are used in acid fast stain?
Carbol fuchsin
acid alcohol
methylene blue
all of the above
When acid fast bacilli stained with acid fast stain , retain Carbol fuschin , so they appear?
blue color
bright red color
orange color
green color
Only calcium that is bound to an anion (such as PO4 or CO3) can be demonstrated by calcium staining?
true
false
Which of the following stains can be used to detect calcium?
Routine stain (H & E)
Von kossa stain
Alizarin red S
Azan stain
All of the above
Which staining is commonly used in histology to detect abnormal deposit of calcium in the body?
Von Kossa staining
Iron stain
Reticulin stain
all of the above
Calcium forms a green black lake with hematoxylin to give a red color on H & E stain?
true
false
What is the clinical application of Von Kossa stain in histology?
blood vessels
articulations
kidneys
all of the above
The staining of lipids is generally by which staining technique in histology?
PAS stain
Iron staining
Calcium staining
fat staining
In which staining, frozen formalin fixed sections are used instead of paraffin embedded sections in histology?
Iron staining
fat staining
routine staining
reticulin staining
What are the common stains in histology used for fat staining?
Sudan lll
oil red O
iron stain
both A & B
Fat stain used for glyceride impart/ shows which color in histology?
red color
green color
blue color
orange color
Fat staining like , Sudan black B - stains which color in histology?
blue color
black color
red color
green color
What is the process by which all nicks and irregularities in the cutting edge of a knife are removed?
stropping
honing
drilling
brushing
What are the sharpening stones used in the process of honing the knife of the Microtome called?
strops
hones
Jagged edge cause lines or tears in section cutting?
true
false
What are the different types of honing?
Manual procedure
automatic procedure
both A & B
none
Which hone is best for manual honing of Microtome knife?
belgian black vein
arkansas
carborundum
plate glass
Which hone is much coarser than other hones?
Belgian black vein
carborundum
arkansas
plate glass
Which hone is used for badly nicked knives?
arkansas
belgian black vein
carborundum
plate glass
Which hone consists of glass disc or wheel driven by an electric motor?
machine hone
belgian black vein/ Belgium yellow
arkansas
plate glass
In honing, the knife is placed at one end of the hone and pushed diagonally forward with a cutting edge. Which motion is used in honing?
head to toe motion
back end motion
figure of 8 motion
none
Honing process is repeated minimum ------ strokes or until the ragged edges are removed in order to sharpen the microtome blade /knife?
100 strokes
10-20 strokes
50-60 strokes
3-5 strokes
Which of the following statement is incorrect regarding precautions taken during honing the knife of a Microtome?
The hone should be lubricated with oil before using
blade must be kept perfectly flat while honing
pressure should be gentle and steady
after honing , oil should not be wiped off from the knife with xylene
Which of the following statement is incorrect?
1. Stropping is the process of polishing the already fairly sharpened edge after honing
2.Stropping is the final technique for sharpening the knife before cutting sections
3. It brings a somewhat sharp blade to like new condition
4. A paddle strop made up of horse leather is preferred in stropping
1&2
2 & 3
3 & 4
none
Which type of strops are preffered due their ease?
rigid type
flexible type
hanging type
all of the above
Which type of strops are fixed on wooden piece with handle?
rigid type
flexible type
circular type
cubical type
Which of the following statement is true?
1.During stropping , a small quantity of vegetable oil is applied beneath the leather to keep the strops soft.
In the stropping method , the movement is that of 'toe to heel' ?
1
2
1 & 2
none
Which of the following statement is true?
the action of stropping is same as honing
in stropping of knife , about 12 strokes are required
preliminary stropping is done on the canvass side
all of these
In stropping method, the movement is that of ----- ?
heel to toe
toe to heel
Which of the following statement is false?
the knife edge should be oiled or greased after use to prevent rusting
the knife should be washed and flushed with xylene
both A & B
none
Which of the following is true about cytology?
study of cells of body under a microscope
study of tissues of body under a microscope
study of microorganisms in a culture plate
study of macroscopic anatomy of body organs
Which of the following statement is correct?
cytopathology is slower than histopathology
cytopathology is the rapid test than histopathology
both are of almost same speed
depends on the specimen
All are the advantages of cytopathology except?
simple & inexpensive
rapid technique
no risk to the patients
need confirmation by histopathology
Which of the following are advantages of cytopathology?
replace frozen section or biopsy
confirmation by histopathology
determine the size and type of lesion
interpretation based on overall cell/ tissue observation
In exfoliative cytology, which types of samples are collected from the body?
urine
CSF
sputum
all of the above
What do you mean by exfoliative cytology?
a technique of cytology in which palpable lesions are examined
a technique of cytology in which cells that spontaneously shed in body fluids are examined
a technique of cytology in which scrapings are examined
none
Which of the cytological technique takes samples by scraping?
exfoliative cytology
abrasive cytology
FNAC
both B & C
What is the other name of FNAC?
Fast and narrow aspiration cytology
fine needle aspiration cytology
fine needle abrasive cytology
free and numb abrasive cytology
By which of the following cytology techniques , specimen are collected from deep organs?
abrasive cytology
exfoliative cytology
fine needle aspiration cytology
both A & B
Which of the following is true?
All liquids (urine , body cavity fluids etc.) have to be centrifuged
for FNAC and thick cellular discharges , centrifugation is must
the sediment of the centrifuged liquid must be discarded and must not be used for smear preparation
all of these
What should be the speed at which the liquid samples must be centrifuged before smearing?
4000
3000
2000
1000
Which of the following should be added to coagulable fluids to prevent coagulation in cytopathology?
xylene
EDTA
Formaline
None
Exfoliative cells degenerate rapidly. In delay , these specimens should be fixed in ----- and refrigerated until smear is prepared?
10% alcohol
95% alcohol
20% alcohol
all of the above
Which of the following is added to preserve the samples like urine and other fluids that needs centrifugation in cytopathology?
10% formalin
40% ethyl alcohol
50 % ethyl alcohol
none
Which of the following is used as an adhesive to adhere the body fluid specimens to the glass slide?
Mayer's egg albumin
EDTA
Citates
xylene
Which of the following are types of stains commonly used in different laboratories in cytopathology?
papanicolaou stain
Diff Quick (DQ) stain
H & E stains
all of the above
Which of the following stain is more superior for demonstrating the nuclear details, that are most important and specific in making the diagnosis of malignancy?
h & E stain
Diff quick (DQ) stain
Modified ZN stain
Papanicolaou stain
Which stain has advantage of both papanicolaou and modified Romanowsky stain in cytopathology?
PAS stain
H & E stain
Modified ZN Stain
Fouchet's test
Which stain needs one minute to perform and is used for microorganisms , cytoplasm and background material staining?
H & E stain
Modified Romanowsky stain
PAS stain
papanicolaou stain
In which stain, slides are fixed in basic ethanol- based solution in cytopathology?
H & E stain
Modified Romanowsky stain
Papanicolaou stain
Diff quick (DQ) stain
Which of the following is true about PAP stain?
used to differentiate cells in gynecological specimens
includes only basic dyes
cannot stain nucleus
contains only one dye
What are the objectives of papanicolaou stain in cytopathology?
definition of nuclear details
transparency of cytoplasm
differentiation of cells
all of the above
What is the color imparted to cell nuclei by the hematoxylin in PAP staining?
orange
black
Pink
Blue
