WorksheetsClinical biochemistry part 1 : Advanced - (1-3)
Total questions: 77
Worksheet time: 39mins
Only increased level of sugar in blood can cause disease?
true
false
Select the correct option?
increased blood sugar- hypoglycemia
decreased blood sugar- hyperglycemia
increased blood sugar- hyperglycemia
none
The function of insulin to?
helps in the excretion of glucose
transport glucose from cells to blood
transport oxygen from blood to cells
transport glucose from blood to cells
Diabetes mellitus is caused due to?
deficiency of insulin
resistance of insulin
over activity of insulin
both A & B
Which of the following blood sugar estimation tests are done in pairs?
A. fasting plasma glucose test
B. random plasma glucose test
C. postprandial
all three
C & A
B & C
A & B
Raju, as advised by Dr. Virendra, has to fast for 8 hours before giving sample for blood glucose estimation. Which of the following glucose tests would Raju must have given sample for?
fasting plasma glucose test
random plasma glucose test
postprandial
none
The normal value of fasting blood sugar is?
<100mg/dl
>100mg/dl
100-140 mg/dl
<200 mg/dl
A patient is considered diabetic when is random blood glucose is -----?
<200 mg/dl
100-140 mg/dl
>126 mg/dl
>200 mg/dl
The different ways of estimation blood glucose in plasma are based on the?
properties of glucose
amount of glucose
source of glucose
all of these
The most commonly used method for the blood glucose estimation is?
folin-wu method
glucose oxidase peroxidase method
glucometer
none
The glucose oxidized by glucose oxidase (GOD) to give?
carboxylic acid
gluconic acid
glycolic acid
glycerol
The color of quinoneimines dye , observed in the GOD-POD method of blood glucose estimation is -------in color?
purple
pink
yellow-orange
red
The intensity of colour quinoneimine dye formed in GOD-POD method of blood glucose estimation is?
indirectly proportional to the glucose concentration in the serum
proportional to the glucose concentration in the different organs
proportional to the glucose concentration in the serum
indirectly proportional to the glucose concentration in the different organs
Sodium fluoride vials are used in GOD-POD methods of blood glucose estimation as , NaF------?
aids glycolysis
prevent glycolysis
preserves blood
none
Buffer enzymes reagent used in blood glucose estimation contains which of the following?
GOD
POD
4-AP
Sodium azide
all of these
Glucose reagent in GOD POD method of blood glucose estimation is added in which of the following test tubes?
blank
test
standard
all of these
The intensity of dye formed in GOD POD is measured at?
530 nm blue filter
500 nm green filter
530 nm red filter
530 nm green filter
In hexokinase method of blood glucose estimation?
ATP is converted into ADP
ADP is converted to ATP
Glucose 6 phosphate gives -------in the presence of G-6-PD?
6-phosphogluconate
6-phosphopyruvate
both A & B
none
In hexokinase method of blood glucose estimation , increase in optical density is measured at an interval of?
5-10 min.
10-15 min.
1-3 min.
30-60 sec.
In hexokinase method of blood glucose estimation, the increase in O.D /min. is directly proportional to?
glucose in standard solution
glucose in specimen
glycolysis in specimen
none
In hexokinase method of blood glucose estimation , glucose reagnet is taken in a ?
test tube
beaker
glass bottle
cuvette
Which performing hexokinase method of blood glucose examination, the following procedure is done-
Pipette 1.0ml of glucose reagent in a cuvette and place it in the incubation block of photometer (37 degree celsius) or keep it in a water bath at 37 degree celsius for 1 min.
true
false
GTT is used for the diagnosis of?
diabetes mellitus
insulin resistance
impaired beta cell function
reactive hypoglycemia
all of these
Types of GTT depends on-
source of glucose
type of glucose
route of glucose
all of these
OGGT and IVGTT are types of GTT. Which of these is more common?
OGGT
IVGTT
GTT is specifically performed when?
confirm or exclude diagnosis of diabetes mellitus when fasting blood glucose test result is not definitive
during pregnancy to detect gestational diabetes
both A & B
none
OGTT test is preferably conducted in the evening?
true
false
The sample required in OGTT is?
blood
urine
plasma
CSF
Urine samples are collected at 30 min. intervals for at least 2 hours and blood sample is collected only once?
true
false
In OGTT , qualitative test of urine sample is done to detect the presence of------?
glucose
ketone bodies
both A & B
none
A curve is plotted by plotting plasma glucose level on X-axis and time on Y-axis , which is called glucose tolerance curve (GTC).
true
false
The normal range of glucose after 1 hour of glucose administration is?
>160 mg/dl
<160 mg/dl
>100 mg/dl
<100 mg/dl
A patient will have an impaired glucose tolerance when his blood glucose level after 2 hours of glucose administration is?
200-250 mg/dl
100-140 mg/dl
140-199 mg/dl
250-300 mg/dl
Fasting blood glucose observed in a diabetic individual in OGTT is?
>126 mg/dl
<126 mg/dl
>150 mg/dl
none
What is the significance of using the 100mg/dl glucose standard solution in the GOD-POD method of glucose estimation?
it helps set the colorimeter wavelength
it determines the maximum optical density
it serves as a reference for glucose concentration
it is used to calibrate the micropipette
If 100 mg/dl of glucose standard solution is used in the procedure of glucose estimation & the color obtained in the test tube containing unknown test solution is lighter than that of the standard test tube, which of the following does it indicate?
the glucose concentration in test solution is more than 100 mg/dl
the glucose concentration is test solution is less than 100 mg/dl
the glucose concentration in test solution is inaccurate
glucose is not all present in the test solution
WHat is the formula for estimating the concentration of glucose in the GOD-POD method of glucose estimation?
OD of test/OD of black * standard concentration
OD of standard - OD of blank/ OD of test * standard concentration
OD of test - OD of blank/ OD of standard - OD of blank * standard concentration
OD of test - OD of standard/ OD of blank * standard solution
Suppose when glucose estimation is done using the GOD/POD method, the results come out to be 30 mg/dl , then which of the following does it indicates?
normal blood sugar level
hyperglycemia
impaired fasting glucose
hypoglycemia
Which of the following are the normal values for fasting blood glucose levels in an adult?
70-110 mg/dl
70-140 mg/dl
50-100 mg/dl
70-210 mg/dl
What is the time period & temperature at which the sample is kept for incubation after the addition of glucose- oxidase peroxidase reagent in the GOD/POD method of glucose estimation?
5 minutes at room temp.
30 minutes at room temp.
10 minutes at room temp.
30 minutes at room 0 degree celcius.
Which of the following is not a component of the GOD - POD method of glucose estimation?
glucose oxidase
peroxidase
chromogenic substrate
hydrochloric acid
Which of the following is the most common chromogenic substrate used in the GOD-POD method?
4-aminoantipyrine and phenol
O-dianisidine
Guaiacol
all of the above
What is the significance of the blank cuvette in the GOD-POD method?
to zero the spectrophotometer or colorimeter
to account for the absorbance of the reaction mixture without the plasma sample
both A & B
none
The optical density of the colored quinoneimine solution is proportional to which of the following?
the concentration of glucose in the sample
the concentration of hydrogen peroxide in the sample
the concentration of the chromogenic substrate in the sample
all of the above
Urea is the best metabolic waste product of-----?
protein
fat
carbs
all of the above
The given option are the metabolic waste product excreted out by the kidney except?
urea
creatinine
phosphoric acid
uric acid
all of the above
The only purpose of the kidney is to filter blood?
true
false
Medically speaking, which term refers to the kidney function?
hepatic
renal
dialysis
urinary
Which of the following is not a function of the kidney?
ensure body does not become too acidic or too basic
produce erythropoietin hormone
storage of metabolic waste product of nucleic acid
activates vitamin D
Which of the following we need to analyse to check the kidney function test?
analysis of urea
analysis of creatinine
analysis of uric acid
all of the above
Clearance test is performed for?
urea
uric acid
creatinine
both A & C
Why is low urea nitrogen is dangerous?
it can indicates postrenal failure
it can indicate renal azotemia
it can indicate prerenal azotemia
it can indicate liver failure
In which organ of the body ammonia is generated?
in some organs of the body during the metabolism of carbohydrates
in all tissues of the body during the metabolism of a variety of compounds
in few tissues during the metabolism of a variety of compounds
none
Where does the ammonia get converted into urea in a human body?
liver
pancreas
stomach
colon
Which of the following is not indicated by elevated urea nitrogen level?
liver failure
prerenal failure
intrinsic renal failure
postrenal failure
Which of the following condition shows high level of urea in the blood?
congestive heart failure
malnutrition
pregnancy
both A & C
What is blood urea nitrogen?
a type of nitrogen only found in the blood urea
a test to assess the levels of nitrogen in urea
the nitrogen from the kidney that can be measured in blood urea
the levels of nitrogen from protein breakdown found in blood
What can high levels of blood urea nitrogen indicates?
high protein diet
kidney disease
internal bleeding
tissue damage
all of the above
What can low levels of blood urea nitrogen indicate?
too much exercise
low protein diet
dehydration
all of the above
Which of the following organ is most likely to be impaired if blood urea nitrogen levels are high?
kidney
lungs
digestive
none
Which of the following method are not used to determine the serum urea nitrogen level?
diacetyl monoxime method
rate of reaction method
berthelot reaction method
none
Which of the given method is used most commonly to determine urea nitrogen level in blood?
rate of reaction method
diacetyl monoxime method
berthelot reaction method
What will be color of complex formed by DAM method?
red colored complex
orange colored complex
blue colored complex
reddish blue colored complex
The intensity of colored complex is measured at?
420 nm
520 nm
620 nm
660 nm
For how long urea is stable in serum?
2 days
3 days
4 days
5 days
At what temperature the urea will be stable in serum for days?
at 4-8 degree C
3-6 degree C
2-8 degree C
at room temperature
In which of the following coloured vial should be containing sample for DAM method for urea nitrogen estimation?
red colored vial
yellow colored vial
black colored vial
blue colored vial
How long the reagents used in DAM method will be stable and can be stored?
till one month
till 1 year
till expiry date
all of the above
What is the correct formula to calculate the urea nitrogen level in blood?
O.D of test/ O.D of std. * 40
O.D of test/ O.D of std. * 30
O.D of test/ O.D of std. * 10
O.D of test/ O.D of std. * 20
Urea standard is used in which of the following prepared test tube to determine urea nitrogen level?
test
blank
standard
all of the above
Which of the following test tube carries sample for the determination of urea nitrogen level?
standard
test
blank
all of the above
Test (T), standard(s), Blank (B) three of the test tube prepared for urea nitrogen level contain which of the following reagent?
urea reagent
acid reagent
DAM reagent
all of the above
Normal reference value for BUN (urea nitrogen) is?
15-20 mg/dl
10-20 mg/dl
5-10 mg/dl
5-20 mg/dl
Which of the given range if average for serum, plasma urea nitrogen level?
14-40 mg/dl
15-20 mg/dl
10-30 mg/dl
20-40 mg/dl
During the estimation of urea nitrogen level, after mixing all the reagents in the test tubes at what temperature we need to keep the test tube in water bath?
120 degree C
100 degree C
70 degree C
90 degree C
How long the prepared test tube should be placed in water bath for proper estimation of urea nitrogen level?
20 min.
15 min.
10 min.
5 min.
