Worksheets2026 Second Semester Biology Practice Final
Total questions: 164
Worksheet time: 2hrs 36mins
Nathan is curious about how animals, plants, water, and sunlight all affect each other in a forest. He wants to know what field of study would help him understand the interaction of the living and non-living parts of an ecosystem. What is this field called?
the study of the interaction of animals with plants
the study of plants
the study of life
the study of the interaction of the living and non-living parts of an ecosystem
Lily is learning about the natural world in her science class. She wants to understand how living things are organized, from the largest group to the smallest. Which shows the levels of organization from largest to smallest?
Biomes, Biosphere, Ecosystems, Communities, Populations, Organisms
Biosphere, Ecosystems, Biomes, Communities, Populations, Organisms
Biosphere, Biomes, Ecosystems, Communities, Populations, Organisms
Organisms, Populations, Communities, Ecosystems, Biomes, Biosphere
Noah is studying the number of gazelle and lions in the savannah over time, as shown in the illustration below. Which claim is supported by the predator and prey illustration?
More predators allowed for more prey to survive in a given area
When there are more gazelles present there can be more lions present in the next few months
The is no correlation (relationship) between predators and prey
When there are more lions present there can be more gazelles present in the next few months
Jessica is studying a local forest ecosystem for her biology project. She wants to find out the maximum number of deer that can be sustained by the forest without depleting its resources. The term for this maximum number is _____________.
Density dependent limiting factors
Biotic Capacity
Carrying capacity
A limiting factor
Kelsey is studying a local ecosystem where owls eat mice and mice eat grass and grains. If the owl population suddenly grows, then most likely
the number of mice will decrease and the amount of grass will not change.
the number of mice will increase and the amount of grass will not change.
the number of mice will increase and the amount of grass will increase.
the number of mice will decrease and the amount of grass will increase.
Casper is studying two different areas in a forest. In area A, he observes that life starts on bare rock with no soil, and over many years, different plants and trees gradually appear. In area B, he notices that after a fire, grasses and small plants quickly grow back because the soil is still present, and eventually, the forest returns. What is the main difference between the two ecological concepts Jackson is observing?
Both show primary succession and the gradual changes that occur when climate changes
Both types of succession shown happens after a man made disturbance
Primary succession in picture A is different from secondary succession in B because it lacks soil and takes longer than secondary succession to occur
Primary succession in picture B is different from secondary succession in A because it lacks soil and takes longer than secondary succession to occur
Henry is studying an energy pyramid in his science class. He notices that as energy moves from the grass to the rabbits, then to the snakes, and finally to the hawk, some energy is transferred while some is lost at each step. About how much energy is transferred to each trophic level and how much energy is lost?
90% transferred and 10% lost
10% transferred and 90% lost
0% lost and 100% transferred
50% transferred and 50% lost
Liam is studying world population trends for his science project. In 2023, he notes that the current human population is ABOUT 8 billion people. According to the figures below, what type of population growth do humans show and is that type of growth sustainable for the human population (in other words can the human population keep growing like that)?
Humans show a logistic growth curve and have sustainably reached their carrying capacity
Humans show an unsustainable exponential growth curve
Humans show an exponential growth curve and have sustainably reached their carrying capacity
Humans show a sustainable exponential growth curve
Zoe and Sam are investigating the relationship between human population density and biodiversity. The amount of forest cover was used as a measure of biodiversity in an area. The students averaged human population density and forest cover from locations with a range of population densities. The graph shows the students’ results. Based on the graph, if the population density increases from 1 to 500 people/km2, the forest cover will likely
Eli is studying how different animals are related by looking at the hemoglobin protein, which helps transport oxygen in red blood cells. He finds a table showing the number of amino acid differences in hemoglobin between humans and three other species. Which statement is supported by evidence in the data table below?
Humans are more closely related to species A since that species has the least number of amino acid differences.
Species C is more closely related to humans since it has the most number of amino acid differences.
Humans are more closely related to species B since there are only 17 amino acid differences.
Species A and C are more closely related since they only have 31 amino acid differences.
Sereen is studying a chart that compares amino acid sequences in hemoglobin from eight different mammals. She notices that each column in the chart is labeled with a number. What do these numbers represent on the chart?
Location of each nucleotide in a DNA sequence
It does not mean anything to a molecular biologist it is just simply a place holder
Location of the amino acid in the polypeptide
Location of the fatty acids in the lipids of the organisms
Hudson is studying a chart that compares the hemoglobin sequences from eight different mammals. The chart uses LETTERS to represent certain components in the sequences. What do the LETTERS represent on the chart below?
Nucleotides
Monosaccharides
Amino acids
Fatty Acids
Juan Pablo is studying a chart that shows the amino acid sequences in hemoglobin from eight different mammals. What is Juan Pablo comparing on the chart below?
The cytochrome-c protein between species
The hemoglobin protein within a population
The hemoglobin protein between species
The cytochrome-c protein within a population
Archer is studying the genetic relationships between different animals for his biology project. He wants to know: In this example, which organism would be MOST related to the human?
Bear
Chimpanzee
Mouse
Shrew
A certain species of rabbit exhibits a lethal condition when the individual carries 2 recessive alleles (think naked Mr. Bigglesworth). If a male rabbit is a heterozygous carrier for a lethal recessive disorder, and a female rabbit is also a hybrid for the trait, what are the chances that their offspring will exhibit (show) the lethal condition?
0%
25%
50%
100%
In a forest, a population of rabbits shows differences in fur color, ear length, and running speed. Why is it ADVANTAGEOUS for this population of rabbits to have such variation?
Variation means the organisms are better able to hide in different areas of their habitat.
Variation makes it easier for organisms to recognize members of their own families.
Variation increases the likelihood that at least some individuals will survive if there is a change in the environment.
Variation in a population provides males with a wider selection of females when mating.
Charli is visiting a natural history museum and sees an exhibit comparing the embryos of different vertebrates, like fish, salamanders, tortoises, chickens, pigs, cows, rabbits, and humans. She reads that embryological comparisons show that:
gorillas begin life as a fish and then develop into gorillas.
as they develop into fetus stage they look more similar showing evidence of common descent
all vertebrate embryo’s look alike in their early stages, and provide evidence for descent with modification
rabbit embryos look like adult fish.
Matthew is visiting a natural history museum and sees an exhibit comparing the evolutionary relationships between different animals. He wonders: Which two organisms are the LEAST closely related?
Human and a Macaque
Human and a chicken
Human and a frog
Human and a lamprey
Eisley is studying a phylogenetic tree in her biology class. She wonders what this tree shows about the evolutionary relationship between all the organisms in the tree. What does the phylogenetic tree reveal?
The close relationship between vertebrates (having a backbone) and invertebrates (not having a backbone)
It shows the evolutionary relationships of living things that have derived (come) from a common ancestor (common descent).
It shows that all vertebrates have hair
All fish have jaws
During a study at a local farm, Aiden observed a population of bunnies over several generations. He tracked the frequency of two alleles in the gene pool. What happened to the allele frequency of the alleles in the gene pool?
The dominant allele frequency increased while the recessive allele frequency decreased in the population
The dominant allele frequency decreased while the recessive allele frequency increased in the population
Both alleles decreased in the population
The allele frequency did not change during the simulation
Miles and Boone are breeding rabbits as part of a genetics experiment. Both of their rabbits have the genotype Ff, where the combination ff results in a lethal condition. What fraction of their offspring will exhibit the lethal condition?
100%
75%
50%
25%
Lucy and Audrey are breeding rabbits as part of a science project. They have a pair of rabbits with the genotype Ff (where F represents furred and f represents naked). What are the phenotype ratios for this genetic cross?
3/4 Furred bunnies, ¼ naked bunnies
2/4 furred rabbits, 2/4 naked rabbits
¼ FF, 2/4 Ff, ¼ ff
3/4 FF , ¼ ff
Graelyn and Madi are breeding their pet rabbits, both of which have the genotype Ff for fur. What are the genotype ratios for this genetic cross?
3/4 Furred bunnies, ¼ naked bunnies
2/4 furred rabbits, 2/4 naked rabbits
¼ FF, 2/4 Ff, ¼ ff
3/4 FF , ¼ ff
In a population of rabbits, some individuals leave behind more living offspring than others, showing a greater reproductive success. These rabbits would be considered to have a high degree of ____________.
Overproduction of offspring
Sexual Selection
Fitness
Genes
In a population of rabbits, natural selection acts on the _____________ rather than on gene combinations that produce that trait.
Genotype
Recessive genotype
Phenotype
Mutations
In a population of rabbits living in a forest, what is the ULTIMATE cause of variation among the rabbits?
asexual reproduction
sexual reproduction
mutation to genes
the cell cycle
Quinne is organizing a massive library of books and wants to categorize them from the broadest group down to the most specific. She wonders: which taxon level would include all levels of classification?
Family
Domain
Kingdom
Genus
Species
Abigail is exploring a tide pool and discovers an unusual organism attached to a rock. She observes that it is eukaryotic, multicellular, and strictly heterotrophic. The organism filters food from the water around it, lacks cell walls, and remains anchored to the sea floor. Abigail learns that it can reproduce by fragmentation but usually reproduces sexually, and that these organisms are often hermaphrodites, with eggs remaining inside the body while sperm is released into the water. Based on these characteristics, to which kingdom does this organism belong?
Animalia
Eubacteria
Archaebacteria
Plantae
Fungi
Carson is studying a tiny organism he found in the digestive tract of chickens and lizards. He observes that it is strictly unicellular, has cell walls made of peptidoglycan (protein-sugars), lacks a nucleus, and contains circular DNA. He also notices the presence of plasmids, which can help the organism resist antibiotics. Based on these characteristics, to which kingdom does this organism belong?
Eubacteria
Animalia
Plantae
Fungi
Protista
Kaiya is examining a sample under the microscope in her biology class. She observes that the organism is multicellular, eukaryotic, and has cell walls made of cellulose. She also notes that it is strictly photoautotrophic and will die without a source of carbon, water, and light energy. Which kingdom does this organism belong to?
Plantae
Animalia
Fungi
Protista
Archaebacteria
Jaylyn is organizing her collection of living things and wants to group them so that each group contains only one specific type of organism. At which taxon level would Abigail find that each group includes only one type of organism?
Family
Domain
Kingdom
Genus
Species
Ruby is observing a sample under the microscope in her biology class. She notices a unicellular organism that has a nucleus, organelles, and performs photosynthesis. The organism also has a flagella, which allows it to swim into the light and evade predators. Based on these characteristics, to which kingdom does this organism belong?
Protista
Animalia
Plantae
Fungi
Archaebacteria
Elliott is a scientist studying organisms that live in extremely salty environments. She discovers a unicellular organism that is prokaryotic and has cell walls without peptidoglycan (protein-sugars). Interestingly, this species can cause waterways to become pink. Based on this information, to which kingdom does this organism belong?
Archaebacteria
Animalia
Plantae
Fungi
Protista
Emily is working in a biology lab and observes the organism shown in the images above under a microscope. She notes that this organism is unicellular, but learns that the kingdom it belongs to also includes multicellular organisms. It is strictly a eukaryotic heterotroph with a cell wall made of chitin. Grace also discovers that these unicellular organisms can reproduce both sexually and asexually. Based on this information, to which kingdom does this organism belong?
Fungi
Animalia
Plantae
Protista
Archaebacteria
Andi is studying how different types of cells are produced in animals. She learns that somatic cells are normal body cells like skin cells and liver cells, while sex cells are the egg and sperm. She discovers that sex cells are only produced in specific locations, but somatic cells are produced everywhere else in the body. In animals, ___________ are produced by mitosis and ________ are produced by meiosis.
gametes , somatic cells
clones , somatic cells
somatic cells , gametes
body cells, zygotes
Emma noticed that her family members have different eye colors, even though they are all related. What are the different versions of a gene that cause these variations called, and where do they come from?
homozygous dominant due to crossing over
homologous chromosomes due to fusion of egg and sperm
alleles due to mutations during DNA replication
sister chromatids due to succession
Cooper is learning about genetics in school. His teacher explains that the sequence (order) of ________ codes for his traits. What is Cooper learning about?
the sugar phosphates
nucleotides
fatty acids
phosphates only
Justine is building a model of a DNA ladder for her science project. She wants to know: what are the sides, the "backbone" of the DNA ladder made up of?
Sugar and nitrogenous bases
Nitrogenous bases and phosphates
Phosphates and proteins
Sugar and phosphates
Julianne is helping her younger brother with his biology homework. She shows him the DNA image below and asks, "Do you remember the complementary base pairing rules?
Adenine always bonds with Guanine
Adenine always bonds with Cytosine
Adenine always bonds with Thymine
Guanine always bonds with Cytosime
Emme is helping her younger sister with her biology homework. She shows her a diagram of a DNA strand, where one of the bases is labeled 'G'. Emma asks, "According to the complementary base pairing rule, which base should pair with 'G'?" What should G base pair with?
Guanine always bonds with Cytosine
Guanine always bonds with Adenine
Guanine always bonds with Thymine
Adenine always bonds to Thymine
Alyse has two pea plants in her garden. She knows the genetic makeup (genotype) of each plant. What is the relationship between the genotype and the physical appearance (phenotype) of these pea plants?
Genotype determines the phenotype of an organism
Phenotype determines the genotype of an organism
There is no relationship
Phenotype are the traits that you can see
Cade and his wife are planning to have a child, they are both heterozygous for the trait in the pedigree. The pedigree below illustrates an autosomal recessive inheritance pattern in their family. What is the probability that they will have a child with the recessive trait?
0/4 chance
1/4 chance
2/4 chance
3/4 chance
4/4 chance
Colt and Gertrude are a married couple. Their family pedigree shows an autosomal dominant inheritance pattern for a certain trait. What must the genotypes of Colt (#1) and Gertrude (#2) be in the first generation and why?
#1 = Dd. and # 2 = dd because they have children who have the trait and who don't have the trait
#1 = dd. and # 2 = dd because both parents have the trait
#1 = Dd. and # 2 = Dd because they have at least one child that does not have the dominant trait
You can not know their genotype since it is an autosomal recessive inheritance pattern
Des and Owen were performing a lab to test the relative rate of photosynthesis in spinach plants. They set-up their experiment like the three cups pictured below, and wanted to know the effect of carbon on the rate of photosynthesis. They repeated this process 10 times and shared their data (the number of leaf chads that rose to the surface/min) with other groups. Why was sodium bicarbonate needed in the leaf lab? THINK ABOUT THE REACTANTS OF PHOTOSYNTHESIS.
Autumn and Makayla were performing a lab to test the relative rate of photosynthesis in spinach plants. They set-up their experiment like the three cups pictured below, and wanted to know the effect of carbon on the rate of photosynthesis. They repeated this process 10 times and shared their data (the number of leaf chads that rose to the surface/min) with other groups. What was the most likely reason the spinach disks start floating to the surface of the water in cup 2?
Jenni and Gabi were performing a lab to test the relative rate of photosynthesis in spinach plants. They set-up their experiment like the three cups pictured below, and wanted to know the effect of carbon on the rate of photosynthesis. They repeated this process 10 times and shared their data (the number of leaf chads that rose to the surface/min) with other groups. Which cup(s) would be the control group(s) for this experiment?
Jacob and Auden were performing a lab to test the relative rate of photosynthesis in spinach plants. They set-up their experiment like the three cups pictured below, and wanted to know the effect of carbon on the rate of photosynthesis. They repeated this process 10 times and shared their data (the number of leaf chads that rose to the surface/min) with other groups. Why didn’t the spinach leaf chads rise to the surface in cup 3?
Cyerra and Taylor were performing a lab to test the relative rate of photosynthesis in spinach plants. They set-up their experiment like the three cups pictured below, and wanted to know the effect of carbon on the rate of photosynthesis. They repeated this process 10 times and shared their data (the number of leaf chads that rose to the surface/min) with other groups. Remember in an experiment a constant are the factors that remain the same between the test subjects. What would need to stay constant in this experiment? (check off TWO that apply)
Britany and Ellie were performing a lab to test the relative rate of photosynthesis in spinach plants. They set-up their experiment like the three cups pictured below, and wanted to know the effect of carbon on the rate of photosynthesis. They repeated this process 10 times and shared their data (the number of leaf chads that rose to the surface/min) with other groups. What is the independent variable in this experiment? (Think about what they are actually testing in this experiment)
Dylan and Micah were performing a lab to test the relative rate of photosynthesis in spinach plants. They set-up their experiment like the three cups pictured below, and wanted to know the effect of carbon on the rate of photosynthesis. They repeated this process 10 times and shared their data (the number of leaf chads that rose to the surface/min) with other groups. What is the dependent variable in this experiment?
What is the definition of 'Biosphere'?
consists of all life on Earth and all parts of Earth in which life exists
is the layer of gases that surrounds the planet
is the solid outer section of Earth, including the crust and upper mantle
is the sum of all water on, under, and above the surface of the Earth
What is the definition of 'Biome'?
are regional climate communities on land. Biomes are described in terms of abiotic factors and biotic factors.
are small water bodies found in deserts.
are single species living in isolation.
are only found in the ocean.
What are examples of things that prevent populations from growing like disease, predators, and competition, and depend on how big the population is?
Density-dependent limiting factors
Abiotic factors
Carrying capacity
Random events
What are examples of things that limit a population like drought and floods, but do not depend on how big the population is?
Density-independent limiting factors
Density-dependent limiting factors
Carrying capacity
Biotic potential
What is the gradual change that starts from bare rock and once soil develops by pioneer species, leads to a climax community?
Primary Succession
Secondary Succession
Ecological Restoration
Deforestation
What is it called when communities or ecosystems that already have soil gradually regrow after an event like fire or flooding?
Secondary Succession
Primary Succession
Ecological Evolution
Habitat Fragmentation
What is the maximum number of individuals of a species that an environment can support called?
Primary succession
Biosphere
Density-independent factor
Carrying capacity
Which graph depicts exponential growth in a population?
What is a community?
A habitat with abiotic factors
A very small habitat
All the abiotic factors in a specific area
All the different populations that live together in an area
______(A)______ is evidence that supports the theory of evolution. An example would be looking at the forelimbs of vertebrates like birds and alligators. The wing of a bird and the foreleg of an alligator have the same structure and are considered_____(b)_______structures.
(A) Comparative anatomy , (B) homologous
(A) Comparative biochemistry , (B) homologous
(A) Comparative biochemistry , (B) vestigial
(A) Comparative anatomy , (B) vestigial
(A) Comparative embryology , (B) homologous
Which of the following is contributing to an INCREASE in carbon dioxide in the atmosphere?
deforestation
photosynthesis
oceans
all of the above
the loss of animal species
The main human activity that releases greenhouse gases is
using public transportation and there by reduce the affects of greenhouse gases.
burning fossil fuels
texting on cellphones
eating meat
What are examples of renewable energy sources?
Solar
Wind
Coal
Gas
What are examples of non-renewable energy sources?
Geothermal
Coal
Oil
Hydro
In the 1800's why did CO2 levels increase significantly?
New factories were being built that caused more pollution
The civil war caused more pollution
Scientists figured out how to clean up pollution in the air
It did not increase
Viruses can be treated with antibiotics.
true
false
Bacterial infections can be treated with antibiotics.
true
false
