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Biotechnology 1 Semester 1 Final Exam Review Game

Total questions: 85

Worksheet time: 43mins

Name
Class
Date
1.

Which action best describes the function of the second stop on a micropipette plunger?

a)

To draw up the liquid into the tip.

b)

To eject the pipette tip from the barrel.

c)

To calibrate the volume of the pipette.

d)

To eject the last remaining drop of liquid from the tip.

2.

You need to measure exactly 250 uL. Which micropipette would be the most appropriate choice?

a)

P20

b)

P200

c)

P1000

d)

P10

3.

Convert 450 uL into milliliters (mL).

a)

4.5 mL

b)

0.45 mL

c)

0.045 mL

d)

45 mL

4.

Which of the following is considered the least accurate tool for measuring a specific volume of liquid?

a)

Volumetric Flask

b)

Graduated Cylinder

c)

Micropipette

d)

Beaker

5.

What is the primary purpose of "taring" or "zeroing" a balance before weighing a chemical in a weigh boat?

a)

To ensure the balance is level on the table.

b)

To subtract the mass of the weigh boat so only the chemical is measured.

c)

To convert grams into milligrams automatically.

d)

To calibrate the balance for the day.

6.

Which piece of equipment uses high-pressure steam and heat to sterilize glassware and biohazardous waste?

a)

Centrifuge

b)

Incubator

c)

Autoclave

d)

Fume Hood

7.

You are working with a volatile chemical that releases toxic fumes. Where should you perform this work?

a)

On the open lab bench

b)

In a laminar flow hood

c)

In a chemical fume hood

d)

Next to an open window

8.

According to the pH scale, a solution with a pH of 9 is considered:

a)

Strongly Acidic

b)

Weakly Acidic

c)

Neutral

d)

Basic (Alkaline)

9.

What is the pH of a solution with a hydrogen ion concentration [H+] of 1×10−31 \times 10^{-3} ?

a)

3

b)

10

c)

-3

d)

7

10.

Which of the following best describes the function of a buffer solution?

a)

It changes the color of a solution to indicate pH.

b)

It increases the rate of a reaction.

c)

It resists changes in pH when small amounts of acid or base are added.

d)

It completely neutralizes any acid added to it.

11.

What information does a Safety Data Sheet (SDS) provide?

a)

The price and catalog number of the chemical.

b)

Hazards, handling, storage, and emergency procedures for a chemical.

c)

The experimental protocol for using the chemical.

d)

The expiration date of the specific bottle.

12.

The GHS pictogram featuring a flame over a circle represents which hazard?

a)

Flammable

b)

Oxidizer

c)

Explosive

d)

Corrosive

13.

Which Biosafety Level (BSL) is appropriate for working with moderate-risk agents that are present in the community and associated with human disease (e.g., Staphylococcus aureus)?

a)

BSL-1

b)

BSL-2

c)

BSL-3

d)

BSL-4

14.

When interpreting a line graph, the variable that is manipulated by the scientist (the cause) is plotted on the:

a)

X-axis

b)

Y-axis

c)

Legend

d)

Title

15.

Which type of graph is best suited for showing the percentage breakdown of a whole (e.g., the composition of trash in a lab)?

a)

Line Graph

b)

Bar Graph

c)

Pie Chart

d)

Scatter Plot

16.

A student records the color of a bacterial colony as "bright orange." This is an example of:

a)

Quantitative data

b)

Qualitative data

c)

Statistical data

d)

Numerical data

17.

Calculate the molar mass of Sodium Hydroxide (NaOH). (Atomic masses: Na=22.99, O=16.00, H=1.01)

a)

40.00 g/mol

b)

58.44 g/mol

c)

36.46 g/mol

d)

24.00 g/mol

18.

How many grams of solute are needed to make 500 mL of a 10% (w/v) solution?

a)

5 g

b)

10 g

c)

50 g

d)

100 g

19.

Which formula is used to calculate the volume needed to dilute a stock solution to a lower concentration?

a)

D=m/vD = m/v

b)

C1V1=C2V2C1V1 = C2V2

c)

A2+B2=C2A^2 + B^2 = C^2

d)

pH=−log⁡[H+]pH = -\log[H^+]

20.

You have a 10M stock solution. You need to make 1.0 L of a 1M solution. How much stock solution do you need?

a)

10 mL

b)

100 mL

c)

500 mL

d)

1000 mL

21.

In a salt water solution, the water is considered the:

a)

Solute

b)

Solvent

c)

Precipitate

d)

Buffer

22.

Which unit is not typically used to express concentration in a biotech lab?

a)

Molarity (M)

b)

Percent mass/volume (%)

c)

Micrograms per microliter (ug/uL)

d)

Degrees Celsius

23.

When using a centrifuge, what is the most important safety rule?

a)

Run it with the lid open to watch the samples.

b)

Balance the tubes symmetrically across the rotor.

c)

Use glass tubes only.

d)

Stop the rotor with your hand to save time.

24.

Gel electrophoresis separates DNA molecules primarily based on their:

a)

Size (length in base pairs)

b)

Color

c)

pH

d)

Temperature

25.

In gel electrophoresis, DNA is negatively charged, so it will migrate toward the:

a)

Negative electrode (Black)

b)

Positive electrode (Red)

c)

Center of the gel

d)

Bottom of the well

26.

What does the acronym "PPE" stand for?

a)

Professional Pipetting Equipment

b)

Personal Protective Equipment

c)

Private Protocol Entry

d)

Primary Procedure Elements

27.

If you spill a hazardous chemical on your skin, what is the recommended minimum time to flush the area with water?

a)

1 minute

b)

5 minutes

c)

15 minutes

d)

60 seconds

28.

Which of the following is a quantitative observation?

a)

The solution turned cloudy.

b)

The reaction produced a foul smell.

c)

The temperature rose by 5.2 degrees Celsius.

d)

The precipitate was white.

29.

What is the standard scientific notation for the number 0.0056?

a)

5.6 x 103

b)

5.6x10−35.6 x 10^{-3}

c)

56 x 10-4

d)

0.56 x 10−210^{-2}

30.

A Standard Operating Procedure (SOP) is best described as:

a)

A log of who entered the lab.

b)

A step-by-step set of instructions to perform a specific routine task.

c)

A list of all chemicals in the storage room.

d)

A report of experimental results.

31.

When plotting enzyme activity vs. temperature, the peak of the curve represents:

a)

The point where the enzyme is denatured.

b)

The optimal temperature for enzyme activity.

c)

The point where the enzyme freezes.

d)

The lowest reaction rate.

32.

What happens to an enzyme if it is heated significantly beyond its optimal temperature?

a)

It works faster.

b)

It replicates itself.

c)

It denatures (loses its shape) and stops working.

d)

It becomes a buffer.

33.

If you measure 12.5 mL of water, how many microliters (uL) is this?

a)

125 uL

b)

1,250 uL

c)

12,500 uL

d)

0.0125 uL

34.

Which pictogram represents a substance that is acutely toxic (fatal or toxic)?

a)

Skull and Crossbones

b)

Exclamation Mark

c)

Gas Cylinder

d)

Corrosion

35.

A student falsifies data to make their results match their hypothesis. This is an example of:

a)

Good data management.

b)

Scientific misconduct/Unethical behavior.

c)

Troubleshooting.

d)

Peer review.

36.

Calculate the mass of NaCl needed to make 0.5 L of a 2 M solution. (Molar Mass of NaCl = 58.44 g/mol).

a)

29.22 g

b)

58.44 g

c)

116.88 g

d)

233.76 g

37.

Which of the following is the correct definition of Molarity?

a)

Moles of solute per liter of solution.

b)

Grams of solute per milliliter of solvent.

c)

Moles of solvent per liter of solute.

d)

Grams of solute per liter of solution.

38.

When should you label a container containing a chemical solution you prepared?

a)

After you use it.

b)

Only if you plan to keep it overnight.

c)

Immediately before or after filling the container.

d)

When the instructor checks your station.

39.

Which piece of equipment is used to grow bacterial cultures at a specific controlled temperature?

a)

Autoclave

b)

Centrifuge

c)

Incubator

d)

Refrigerator

40.

What is the dependent variable in an experiment testing how different amounts of fertilizer affect plant height?

a)

The amount of fertilizer.

b)

The type of plant.

c)

The height of the plant.

d)

The amount of water given.

41.

Which of the following pH values represents the strongest acid?

a)

pH 6

b)

pH 13

c)

pH 2

d)

pH 8

42.

Which glassware is designed to hold and mix chemicals but is not precise enough for volumetric measurements?

a)

Volumetric Flask

b)

Erlenmeyer Flask

c)

Volumetric Pipette

d)

Graduated Pipette

43.

To measure 1.2 mL of liquid most accurately, which pipette should you use?

a)

A P1000 micropipette set to 1000 followed by a P200 set to 200

b)

A 10 mL serological pipette

c)

A 5 mL transfer pipette

d)

A P20 micropipette

44.

What defines the "Biohazard" symbol?

a)

Three circles overlapping in a triangle formation

b)

A diamond with a flame

c)

A skull and crossbones

d)

A test tube pouring liquid on a hand

45.

If a solution has a density of 1.5 g/mL and you have 20 mL of it, what is the mass?

a)

13.3 g

b)

30 g

c)

20 g

d)

1.5 g

46.

Which step comes first when using a pH meter?

a)

Dip the electrode into the sample

b)

Store the electrode in distilled water

c)

Remove the electrode from the storage buffer and rinse with distilled water

d)

Calibrate the meter with standard buffers

47.

When diluting a strong acid, why should you always add acid to water, not water to acid?

a)

Adding water to acid can cause splashing and rapid heat generation

b)

Adding acid to water makes the solution weaker

c)

It allows the acid to dissolve slower

d)

It prevents the water from evaporating

48.

Which document would you use to record your daily experimental observations, raw data, and thoughts?

a)

SOP

b)

Lab Notebook

c)

MSDS

d)

Calibration Log

49.

An "Exclamation Mark" pictogram on a chemical bottle indicates:

a)

The chemical is radioactive

b)

The chemical is an irritant or skin sensitizer

c)

The chemical is under high pressure

d)

The chemical causes birth defects

50.

What is the mean (average) of the following data set: 2.0, 2.5, 3.0, 2.5?

a)

2.0

b)

2.5

c)

3.0

d)

10.0

51.

Which scientist is credited with producing "Photo 51" using X-ray crystallography, which was essential in identifying the structure of DNA?

a)

James Watson

b)

Rosalind Franklin

c)

Erwin Chargaff

d)

Frederick Griffith

52.

According to Chargaff's Rule, if a DNA molecule contains 20% Adenine, how much Thymine should it contain?

a)

20%

b)

30%

c)

80%

d)

0%

53.

Which bond holds the two separate strands of DNA together (between the nitrogenous bases)?

a)

Peptide Bond

b)

Covalent Bond

c)

Hydrogen Bond

d)

Ionic Bond

54.

A nucleotide, the building block of DNA, consists of which three components?

a)

Sugar, Protein, and Fat

b)

Phosphate group, 5-carbon sugar, and Nitrogenous base

c)

Amino acid, R-group, and Carboxyl group

d)

Glucose, Water, and Acid

55.

What is the correct complementary DNA strand for the following sequence: 3’ A T C C G A 5’?

a)

5’ A T C C G A 3’

b)

5’ T A G G C T 3’

c)

3’ U A G G C U 5’

d)

5’ G C T T A G 3’

56.

In the context of macromolecules, what is the monomer (building block) of a protein?

a)

Monosaccharide

b)

Nucleotide

c)

Amino Acid

d)

Glycerol

57.

What is the primary function of lipids (fats) in a biological system?

a)

Storing genetic information

b)

Long term energy storage and membrane structure

c)

Speeding up chemical reactions

d)

Transporting oxygen

58.

Enzymes work to speed up chemical reactions by:

a)

Increasing the temperature of the solution

b)

Lowering the activation energy required for the reaction

c)

Changing the pH of the solution

d)

Becoming part of the final product

59.

On an enzyme molecule, the specific area where the substrate binds is called the:

a)

Active Site

b)

Product Zone

c)

Nucleus

d)

Allosteric Site

60.

Which of the following is a strict rule for maintaining a legal scientific notebook?

a)

Use a pencil so you can erase mistakes.

b)

Use blue permanent ink only.

c)

Tear out pages if you mess them up completely.

d)

Leave blank lines between days to make it look neat.

61.

If you attach a graph or data printout into your lab notebook, how should you sign it?

a)

Sign only the back of the printout.

b)

Sign the tape that holds it in.

c)

Sign across the edge of the attachment so the signature extends onto the notebook page.

d)

You do not need to sign attached items.

62.

Why is water considered a "polar" molecule?

a)

It freezes at 0 degrees Celsius.

b)

It has a slight positive charge on the hydrogens and a slight negative charge on the oxygen.

c)

It is found at the North and South Poles.

d)

It contains salt.

63.

What is the main difference between tap water and deionized (DI) water?

a)

Tap water has had all organic contaminants removed.

b)

DI water has had ions (like metals and salts) removed through resin cartridges.

c)

Tap water is more pure than DI water.

d)

DI water contains added fluoride for safety.

64.

When preparing a solution, the substance that is dissolved (usually the solid) is called the:

a)

Solvent

b)

Solute

c)

Buffer

d)

Precipitate

65.

You need to make 100 mL of a 30% (w/v) sucrose solution. How much sucrose do you weigh out?

a)

0.3 grams

b)

3.0 grams

c)

30 grams

d)

300 grams

66.

You are making 100 mL of a 60% (v/v) glycerol solution. After pouring 60 mL of glycerol into the cylinder, how much water should you add?

a)

100 mL

b)

60 mL

c)

40 mL

d)

0 mL

67.

What is the specific purpose of "Autoclave Tape"?

a)

To seal the door of the autoclave shut.

b)

To label the name of the bacteria.

c)

To show if the autoclave actually worked by developing black stripes.

d)

To repair broken glass before sterilizing.

68.

Which pipette is best suited for transferring larger volumes of liquid, such as 10 mL or 25 mL?

a)

P1000 Micropipette

b)

Serological Pipette

c)

Transfer Pipette

d)

P20 Micropipette

69.

What is the primary function of a "Water Bath" in the lab?

a)

To wash dirty glassware.

b)

To incubate samples at a constant temperature for a set time.

c)

To freeze samples quickly.

d)

To mix solutions vigorously.

70.

In a Standard Operating Procedure (SOP), which section defines WHO is responsible for performing the task?

a)

Scope

b)

Purpose

c)

Responsibility

d)

References

71.

Which Biosafety Level (BSL) would contain dangerous agents like Ebola or Smallpox, for which there are no vaccines or treatments?

a)

BSL-1

b)

BSL-2

c)

BSL-3

d)

BSL-4

72.

How are agents in a BSL-3 laboratory, such as Tuberculosis or SARS-CoV-1, typically transmitted?

a)

Through skin contact only.

b)

Via inhalation (aerosols).

c)

By eating contaminated food.

d)

Through water sources.

73.

Identify the correct scientific notation for the number 900,000.

a)

9×1059 \times 10^5

b)

9×1069 \times 10^6

c)

90×10490 \times 10^4

d)

0.9×1070.9 \times 10^7

74.

Which of the following is a "Qualitative" observation?

a)

The bacteria colony is 4mm wide.

b)

There are 12 colonies on the plate.

c)

The bacteria is glowing green.

d)

The incubator is at 37 degrees.

75.

What is the source of "Agarose," the sugar used to make gels for electrophoresis?

a)

Corn starch

b)

Animal fat

c)

Seaweed

d)

Bacterial cell walls

76.

Besides size and charge, what other factor influences how DNA separates in a gel?

a)

Color

b)

Shape

c)

Smell

d)

Texture

77.

When removing gloves, what is the first step to ensure safety?

a)

Pull the fingers of one glove with your teeth.

b)

Grasp the outside of one glove at the wrist without touching bare skin.

c)

Wash the gloves with soap and water before taking them off.

d)

Slide a finger under the wrist of the first glove.

78.

Which piece of safety equipment is best used to smother a grease fire or wrap around a person whose clothing is on fire?

a)

Fire Extinguisher

b)

Safety Shower

c)

Fire Blanket

d)

Fume Hood

79.

What does the "NFPA Hazard Diamond" communicate?

a)

The price of the chemical.

b)

The specific storage location in the room.

c)

The health, fire, instability, and specific hazards of a chemical.

d)

The expiration date of the chemical.

80.

You calculate you need 550 uL of solute. You only have a P1000 micropipette. What should the window read (top to bottom)?

a)

0 - 5 - 5

b)

5 - 5 - 0

c)

0 - 5 - 0

d)

0 - 0 - 5

81.

Which of these chemicals are components of a buffer solution?

a)

Salts

b)

Strong Acids

c)

Strong Bases

d)

Indicators

82.

Which biosafety level characterizes most high school laboratories?

a)

BSL-1

b)

BSL-2

c)

BSL-3

d)

BSL-4

83.

What can an acidic buffer solution no longer hold when it reaches buffer capacity?

a)

OH-

b)

H+

c)

H2O

d)

CO3-

84.

A researcher records enzyme activity at different temperatures and plots the results. The graph shows activity increasing up to 45°C and then decreasing at higher temperatures. What trend does this graph illustrate?

a)

  1. Enzyme activity has an optimal temperature

b)

  1. Enzyme activity is independent of temperature

c)

  1. Enzyme activity is independent of temperature

d)

  1. Enzyme activity decreases at all temperatures

85.

What is the mass of CO2 given off from the yeast treated with 20 grams of sucrose?

a)

0 grams

b)

4 grams

c)

6 grams

d)

9 grams

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