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WorksheetsMicrobiology Worksheet – Zoonoses and Bacterial Diseases
Total questions: 74
Worksheet time: 37mins
The pathogenicity of anthrax is caused by which factors?
the exotoxin, edema factor and protective antigen
adhesion, colonization, enterotoxin production
invasion, histamine‑sensitizing factor
endotoxin, fatty acids of the cell wall
microcolonial, tetanospasmin and tetanolysin
The source of infection in anthrax is mainly:
fleas
pliers
animals
only humans
food supply
For microbiological diagnosis of tularemia, which is used?
bioassay
microscopy of blood smears
Widal reaction
Byrne sample
Dick test
Mode of transmission of tularemia:
direct contact with patients
inoculation
sexual transmission
vertical transmission
inhalation
Resistance of Brucella:
not sensitive to high temperature
preserved during boiling
not sensitive to disinfectants
long‑term storage at low temperature
not sensitive to UV
Allergic method of diagnosis of brucellosis:
Dick sample
Chic sample
test with tularemia
brucellin skin test
sample with antitoxin
Antigens of Brucella include:
Vi‑antigen
protective antigen
H antigen
K antigen
peptidoglycan
Standard agglutination test reveals:
precipitin
agglutinogens
complement‑binding antibodies
thrombocytopathy
hemolysins
Ingestion of the tularemia pathogen causes which form of infection?
intestinal form
pulmonary form
septic form
cutaneous form
generalized form
When the airborne pathway of infection with tularemia occurs, which form develops?
pulmonary form
intestinal form
septic form
cutaneous form
generalized form
When the contact pathway of infection with tularemia occurs, which form develops?
intestinal form
ulcer‑bubonic form
septic form
pulmonary form
generalized form
Immunity in tularemia is:
antitoxic
unstable, weakly expressed
non‑sterile, turning into sterile
transmitted passively from the mother
accompanied by allergy of the body
Allergic method of diagnosis of tularemia:
Dick sample
Chic sample
Mantoux reaction
Burne test
sample with tularin
Specific prevention of brucellosis is carried out using:
neutralization of products and raw materials of animal origin
bacteriophages
killed vaccine
live vaccine
toxoid
When airborne pathway transmission of anthrax occurs, which form develops?
intestinal form
pulmonary form
bubonic form
septic form
skin form
Heddleson reaction is used in the diagnosis of brucellosis in:
bacteria carriers
mass surveys
allergy detection
phage typing
neutralization reactions
Rickettsias are cultivated:
on simple nutrient media
on enriched nutrient media
in chicken embryo fibroblasts
on yolk‑salt sugar
in the yolk sac of a chicken embryo
Borrelia are transmitted:
sexual transmission
through insect bites
alimentary transmission
airborne transmission
inhalation of dust
Borrelia, based on type of respiration, are:
facultative anaerobes
obligate anaerobes
obligate aerobes
facultative aerobes
hematuria
Diagnostic value in Brill’s disease have which serum immunoglobulins?
IgM
IgG
IgA
IgE
The causative agent of Q fever most often affects which organ system?
Gastrointestinal tract
Lungs
Urogenital system
Skin and mucous membranes
The causative agent of recurrent typhus is cultivated on media containing which additive?
Bile
Serum and ascitic fluid
Bismuth
Magenta dye
Salt
Cultivation of pale Treponema is carried out in media containing what component?
Bismuth sulfite
Magenta dye
Glucose
Brain tissue
Bile
The source of infection in syphilis is what host?
People
Cows
Pigs
Birds
Amphibians
Primary syphilis is characterized by which key clinical finding?
Roseolous rash
Gummatous infiltrates in organs
Hard chancre
"Necklace of Venus"
Seborrhea
Secondary syphilis is characterized by which manifestation?
Allergic reactions of cellular type
Nausea
Neurological damage symptoms
Rashes on the skin and mucous membranes
Formation of a chancre
Tertiary syphilis is characterized by which process?
Formation of gummatous infiltrates
Formation of the chancre
Diarrhea
Roseolous rash
Nausea
What structure ensures the infectivity of chlamydia?
Taurus inclusions
Glycogen bodies
Elementary corpuscles
Aggregated bodies
Initial corpuscles
For the cultivation of mycoplasmas, which antibiotic class is added to the nutrient medium?
Beta‑lactams
Aminoglycosides
Macrolides
Tetracyclines
Glycopeptides
Specific prevention of urogenital chlamydia: which statement is correct?
Provides for administration of immunoglobulins
Is not developed
Based on isolation of patients
Is fully effective
Leads to lifelong immunity
Type of respiration of urogenital mycoplasmas is best described as:
Facultative anaerobes
Obligate anaerobes
Psychrophiles
Microaerophiles
Capnophiles
Detection in serum of immunoglobulins M and G against Chlamydia trachomatis antigens indicates:
Acute disease
Chronic infection
Carriage
Post‑vaccination immunity
Convalescence
Urogenital mycoplasmosis of pregnant women most commonly leads to:
Creation of passive immunity in the fetus
Stillbirth
Development of heart defects
Development of the Hutchinson triad
No teratogenic effect
Recurrent typhoid occurs as which clinical course?
Sepsis
Septicopyemia
Recurrent infection
Secondary infection
Reinfection
The source of infection in endemic typhus is:
People
Horses
Goats
Amphibians
Rodents
The vector of infection in epidemic typhus is:
Fleas
Pliers
Mosquitoes
Lice
Bugs
Leptospira grow on nutrient media containing which component?
Bismuth
Serum
Bile
Protein
Casein
What property of Chlamydia trachomatis allows detection inside infected cells?
Ability to cause fusion of infected cells
Simple storage in tissues
Formation of characteristic inclusions
Presence of volutin granules
The vector of infection in endemic typhus is:
Fleas
Ticks
Mosquitoes
Lice
Bugs
Specific prevention of epidemic typhus is carried out:
Toxoid
Phages
Antibiotics
Vaccine
Interferons
The main factor of pathogenicity of chlamydia:
The lipopolysaccharide of the cell wall
Acid resistance
Anti-phagocytic activity
Toxin with a membrane-damaging mechanism of action
Enterotoxin
By the type of respiration Mycoplasma is:
Facultative anaerobes
Obligate anaerobes
Obligate aerobes
Microaerophiles
Capnophile
The genus Ureaplasma differs from the genus Mycoplasma by ability:
Form ammonia from urea
Break down carbohydrates
Form a cell wall
Color by Gram
Grow on nutrient media
The alternation of attacks of fever and anorexia in recurrent typhoid fever is associated with:
The ability of Borrelia to persist in the cytoplasm of phagocytes
A decrease in the immune status
The tendency of Borrelia to lose their pathogenic properties
The formation of L-forms of bacteria
Rapid formation of antibiotic resistance
Four months after the operation, the doctor noted yellowing of the sclera, mucous membranes, and skin and found that the patient was transfused donor blood. What preliminary diagnosis did the doctor make to the patient?
Hepatitis B
Yersiniosis
Hepatitis A
Sepsis
Leptospirosis
After suffering from hepatitis B, hepatocellular carcinoma developed in the patient. In what type of infection is the viral genome included in the genome of the host cell, causing cancer?
Integrated
Latent
Abortive
Acute
Chronic
During the epidemiological examination of the patients, the doctor found out that they were transfused a small amount of donor blood of one series during the treatment. What viral hepatitis is diagnosed in patients?
Hepatitis B
Hepatitis C
Hepatitis A
Hepatitis D (hepatitis Delta)
Hepatitis E
What high-sensitivity reaction should be used to test donor blood for the presence of hepatitis B antigens?
Solid-phase enzyme immunoassay
Radioimmunoassay
Indirect hemagglutination reaction
Complement binding reaction
Indirect immune fluorescence reaction
In a laboratory study of the material from a patient with jaundice, the doctor received the following data: HBsAg positive, HBeAg negative. What diagnosis did the doctor make based on the results?
Hepatitis C
Hepatitis B, history-hepatitis B
Chronic hepatitis B with low replication activity
Relapse of hepatitis B
Repeated infection with hepatitis B
In the genome of the smallpox vaccine virus, scientists have integrated the gene of the hepatitis B virus, which is responsible for the synthesis of HBsAg. The recombinant antivirus is planned to be used in a vaccine preparation. What type of vaccine does the vaccine thus obtained belong to?
Genetic engineering
Combined
Associated
Synthetic
Chemical
After examining the patient and according to the anamnesis, the doctor diagnosed the patient with hepatitis D (Delta). What result of laboratory research can confirm the diagnosis?
Detection of hepatitis B and D markers in blood
Detection of hepatitis A and B markers in blood
Detection of hepatitis E and D markers in blood
Detection of hepatitis C and D markers in blood
Detection of hepatitis F and D markers in blood
The patient has jaundice, fever, lack of appetite, and the liver is increased by 3 cm. What reaction, first of all, must be put to clarify the diagnosis?
ELISA for HBs-antigen detection
Vidal reaction
Weil reaction
Wasserman reaction
RNGA with chlamydia diagnosticum
In a 25-year-old patient, the doctor noted a fever and jaundice. In a laboratory study of the patient’s blood, the doctor did not detect either HBsAg or antibodies to HBsAg. Which of the additional tests can most reliably confirm infection with the hepatitis B pathogen?
Detection of antibodies against HBsAg
Identify HBsAg
Detection of antibodies against HBeAg
Revealing HBxAg
Detection of Delta-antigen
A patient was admitted to the infectious diseases hospital with complaints of general malaise, fever up to 38 °C, and jaundice. Given that a few months ago the patient received a blood transfusion, the doctor made a preliminary diagnosis of viral hepatitis B. What are the main methods of laboratory diagnosis of this disease?
Serological and molecular genetic
Virological: accumulation of virus in cell culture and indication of pathogen by CPD
Detection of virions in blood by electron microscopy
Virological: identification of virus in laboratory animals by neutralization reaction
Virological: accumulation of virus in chicken embryo
When checking the blood of one of the donors, the doctor identified HBsAg. The donor said that he had recently been treated and had been given a parenteral infusion of solutions. Evaluate the quality of donor blood for transfusion.
Blood cannot be used for transfusion
Blood can be used for transfusion
For transfusion, you can only use blood plasma
For transfusion, you can only use red blood cell mass
Blood may be transfused only after pre-treatment
In a patient with severe viral hepatitis, antibodies against the pathogens of hepatitis B and D were found in the blood. It is known that the Delta virus cannot reproduce itself in hepatocytes. By what process is it possible to reproduce the hepatitis D virus in the presence of the hepatitis B virus?
Unilateral complementation of viruses
Interference of viruses
Genetic reactivation of defective viruses
Phenotypic mixing
Mutations in the genome of the Delta virus
A patient presented with subfebrile temperature, malaise, and jaundice. From anamnesis, three months ago the patient underwent intravenous manipulation. The preliminary diagnosis is viral hepatitis. What studies should be conducted to confirm or reject the diagnosis?
Determination of total HBs antigen in the patient's blood serum
Bacteriological examination of the patient's feces
Investigation of nasopharyngeal flush for the presence of the hepatitis A pathogen
Investigation of nasopharyngeal flush for the presence of the hepatitis C pathogen
Determination of the total pool of IgA
A patient was admitted with general weakness, subfebrile temperature, and yellowing of the sclera and skin. Two weeks earlier the patient was resting at sea and eating seafood without thermal processing. Detection of which markers will confirm the diagnosis of acute hepatitis E?
IgM anti-HAV
IgM anti-HEV
IgG anti-HEV
IgG anti-HAV
IgA anti-HEV
Patient M. was admitted with general malaise, fever up to 38 °C, and jaundice. Given that a few months ago he received a blood transfusion, the doctor suspected hepatitis B. What are the main methods of laboratory diagnosis of hepatitis B?
Virus isolation on cell culture and identification by CPD
Detection of virions in blood by electron microscopy
Virus isolation in laboratory animals in RVN (viral neutralization reaction)
Serological and gene diagnostics
Virus isolation on chicken embryo
In a 25-year-old patient, the doctor noted fever and jaundice. In a laboratory study of the patient’s blood, neither HBsAg nor antibodies to HBsAg were detected. Which additional test can most reliably confirm infection with the hepatitis B pathogen in this situation?
Detection of antibodies against HBsAg
Identify HBsAg
Detection of antibodies against HBeAg
Revealing HBxAg
Detection of Delta-antigen
After a laboratory examination of a patient with recurrent opportunistic infections, the doctor diagnosed HIV infection. The result of what study allowed the diagnosis to be confirmed?
Result of ELISA
Result of hemagglutination inhibition reaction
Result of passive hemagglutination reaction
Result of complement binding reaction
In a laboratory study of the patient’s blood serum using ELISA, the virologist identified antibodies to HIV. What additional research is needed to confirm the diagnosis of HIV infection?
The study of blood serum using immunoblotting
Immunofluorescence reaction
In-depth immunological examination
The study of blood serum in the reaction of immunodiffusion
Electron microscopic examination of blood cells
In order to test the blood of donors for the presence of hepatitis b antigens, it is necessary to apply highly sensitive methods. Which of these reactions should be applied to this purpose?
Immunoelectrophoresis
Indirect hemagglutination
ELISA
Complement bindings
Passive immunofluorescence
In a patient, disease is caused by the hepatitis B virus, which interacts with the cell in an integrative type. Specify the characteristic of the interaction of the virus with the cell in an integrative type of infection.
Nucleic acid is embedded in the cell chromosome and exists there as a provirus (can lead to changes in hereditary variability)
The virus reproduction cycle is blocked at one stage and no new virions are formed
The virus in the cell functions autonomously, forming new virions
The virus enters the cell and dies under the action of lysosomal enzymes
The virus enters the host cell nucleus and amplifies the virus
Using immunoblotting, a virologist revealed antibodies to one of the HIV proteins gp41 in a patient with suspected HIV infection. The result was rated as dubious. What research should be done to confirm the diagnosis?
IFA
Examine the immunogram
RSK
The reaction of radioimmunoprecipitation
Rnga
When examining a 16-year-old drug addict, the doctor revealed sharp weight loss, swollen lymph nodes, low-grade fever, and prolonged diarrhea. The preliminary diagnosis is AIDS. Which study will finally confirm the diagnosis?
Immunoblotting
Indirect hemagglutination reaction
Radioimmune analysis
Linked immunosorbent assay
ELISA
In the initial study of the material from the patient, the virologist used an enzyme linked immunosorbent assay and revealed the patient's antibodies to HIV. What should the doctor do next?
Conduct confirmatory diagnostics using immunoblotting
Prescribe antiretroviral therapy
Re-examine the material after 1 year
Accumulate virus in cell culture
Diagnose with tuberculin
In an 18-year-old boy, the doctor revealed an increase in inguinal lymph nodes, prolonged low-grade fever and candidiasis of the oral cavity. Indicate the most reliable laboratory study that will confirm the fact of infection of the patient with HIV infection.
Western blotting
Radioimmune analysis
Enzyme-linked immunosorbent assay for the detection of antibodies
ELISA
Enzyme-linked immunosorbent assay for the detection of antigens
When studying the immune status of an AIDS patient, it is necessary to determine the level of T-helpers. What reaction will make it possible to identify these cells?
ELISA with labeled monoclonal antibodies
The reaction of rosette formation with sheep erythrocytes
The reaction of rosette formation with red blood cells loaded with C3 complement fraction
Rosette reaction with erythrocytes loaded with IgG
ELISA with labeled antibody-lymphocyte immunoglobulins
When examining a young man at the AIDS Center, the patient received a positive ELISA with HIV antigens. Complaints about the patient’s state of health are absent. What does a positive ELISA testify to?
About HIV infection
About AIDS
About infection with the causative agent of hepatitis B
About the past AIDS disease
About the persistence of the hepatitis B pathogen
To diagnose a patient with HIV infection, the virologist examined the blood serum to detect specific antibodies using an enzyme-linked immunosorbent assay. What enzyme-labeled antibodies were used?
Against human immunoglobulins
Against HIV antigens
Against glycoprotein gp120
Against protein p17
Against glycoprotein gp41
In a patient with pneumonia in sputum, a virologist revealed Pneumocystis carinii. Exceptionally, for what infectious disease can this etiological form of pneumonia develop?
For HIV infection
With the plague
With ornithosis
With Legionellosis
With Q fever
A woman, 35 years old, went to the doctor with complaints of general weakness, fatigue and swollen lymph nodes (lymphadenopathy). From the anamnesis, the doctor found out that the patient had been taking drugs parenterally for several years. Serological blood tests (ELISA) revealed antibodies to HIV 1 in a patient. Repeated blood tests gave a positive result. What should be the actions of a doctor to confirm the diagnosis?
Put the reaction of immunoblotting (western blotting)
Immediately examine all sexual partners in order to identify HIV-infected people
The diagnosis does not require confirmation
Put the Vidal reaction
Put Wasserman reaction
At a blood transfusion station in the blood serum of one of the donors, the doctor identified antibodies to the human immunodeficiency virus. What research does a virologist need to apply to confirm the diagnosis of HIV infection?
Western blotting (immunoblotting)
Electron microscopy
Linked immunosorbent assay
ELISA
Radioimmunoassay
