Font size
WorksheetsBiochemistry and Physiology Review Questions
Total questions: 150
Worksheet time: 1hrs 15mins
What is the nature of enzymes?
Protein
Lipid
Coenzyme
Hormone
Which set consists of water-soluble vitamins?
B1, B6, ascorbic acid
Vitamin K, A, C, B1
Vitamin B1, B2, E, and C
Vitamin B2, C, and thiamine
The reaction AB → A + B is catalyzed by which factor?
Hydrolase
Hydratase
Transferase
Catalase
The reaction RCOOH → RH + CO2 is catalyzed by which factor?
Hydrolase
Decarboxylase
Isomerase
Synthetase
Coenzyme A contains which substance?
Riboflavin (vitamin B2)
Pantothenic acid (B5)
Nicotinamide (vitamin PP)
Thiamin (vitamin B1)
Which are the fat-soluble vitamins?
A, B, C, D
A, B, E, K
A, B, D, P
A, D, E, K
Which is an action of vitamin PP (niacin)?
Prevents Beri-Beri
Prevents Seobus disease
Prevents angular stomatitis
Prevents psoriasis
What is the catalytic role of enzymes in reactions?
Decrease activation energy
Increase activation energy
Increase contact between substrate molecules
Prevent reverse reactions
The hypothalamus secretes which type of hormone?
RF
IF
GH
Both A and B
What is an effect of vitamin A?
Prevents Beri-Beri
Prevents scurvy
Converts opsin to rhodopsin
Catalyzes isomerization reactions
How many ATP are formed when electrons are transferred from one FADH2 and one NADH molecule to the final acceptor O2?
3
4
5
6
Which set participates in the composition of NAD?
Adenine, nicotinamide, deoxyribose
Adenylic acid, nicotinamide, ribose
Adenylic acid, pantothenic acid, nicotinamide
Adenine, ribose, thioethanolamine
Which of the following coenzymes does not contain a vitamin?
NAD
FAD
CoA
Lipoic acid
Dehydrogenase enzymes have which coenzymes?
NAD+
NADP+
CoA
FAD
What is the nature of enzymes?
Protein
Lipid
Coenzyme
Hormone
What is the primary role of vitamin B6?
Participates in the visual mechanism
Prevents pellagra
Participates in blood coagulation
Catalyzes transamination and decarboxylation reactions
Which set lists fat-soluble vitamins?
E, A, K, folic acid
D, E, B1, K
K, D, A, tocopherol
A, C, B6, D
Which vitamin participates in the structure of coenzyme A?
Vitamin E
Vitamin B5
Vitamin A
Vitamin B
Deficiency of nicotinamide may cause which disease?
Beri-Beri edema
Scurvy
Pellagra
Corneal keratinization
Which vitamin plays a role in protecting the epithelium?
Vitamin C
Vitamin A
Vitamin B1
Vitamin B12
What is the optimal temperature for enzyme activity in the body?
25°C
30°C
37°C
40°C
At what pH does salivary amylase act optimally?
5
6
7
8
NAD+ and NADP+ are coenzymes of enzymes that catalyze which reactions?
Aminotransfer
Electron exchange
Hydrogen transfer
Transfer of –CH3 groups
In cardiovascular disease, which LDH isoenzyme increases predominantly in blood?
LDH 1
LDH 2
LDH 3
LDH 4
To reach maximal reaction rate, the substrate concentration S should be how many times larger than Km?
10 times
20 times
50 times
100 times
Based on chemical nature, into how many types are hormones classified?
2 types
3 types
4 types
5 types
Which cells secrete insulin?
Pancreatic beta cells
Adrenal medulla
Liver cells
Anterior pituitary
Where are steroid hormone receptors located?
On the cell surface
Within the cell membrane
Inside the cell
In mitochondria
Pancreatic hormones belong to which group?
Peptides
Glucids
Amines
Steroids
Thyroid hormones are synthesized from which source?
Free tyrosine
Tyrosine within albumin
Tyrosine within globulin
Tyrosine within fibrin
The adrenal medulla secretes which hormone?
Mineralocorticoid
Glucocorticoid
Insulin
Catecholamine
What is the function of a receptor?
Acts like an enzyme
Binds specifically to a hormone
Forms a pore to let a specific hormone pass through
Forms a complex to increase hormone solubility
What is the significance of anabolism and catabolism?
Provide new materials for cell construction
Help provide energy for activity
Enable cells of organisms to function normally and maintain life
All of the above
What is phosphorylation?
Breaking a phosphate bond
Adding a phosphate group to an inorganic molecule
Adding a phosphate group to an organic molecule
An energy-releasing reaction
Which statement best defines an oxidizing agent?
Donates electrons; its element’s oxidation number increases after the reaction
Donates electrons; its element’s oxidation number decreases after the reaction
Accepts electrons; its element’s oxidation number increases after the reaction
Accepts electrons; its element’s oxidation number decreases after the reaction
Which pair of concepts are equivalent?
Oxidation process and oxidation
Oxidation process and oxidizing agent
Reduction process and oxidation
Oxidation process and reducing agent
How are electrons transferred from one molecule to another?
Directly, by hydrogen atoms, hydric iron; directly bound to oxygen
Indirectly, directly, by hydrogen atoms, directly to oxygen
Indirectly, directly, by carbon atoms, by hydrogen atoms
Directly, by hydrogen atoms, by carbon atoms, by hydrogen atoms
What is the first substance entering the tricarboxylic acid (TCA) cycle?
Acetyl-CoA
ATP
NADH
Glycolysis
Which compound is not a high-energy compound?
Glucose 6-phosphate
Phosphoenolpyruvate
Acetyl-CoA
1,3-Bisphosphoglycerate
Which of the following is a high-energy bond?
Glycosidic bond
Ester phosphate bond
Peptide bond
Acyl phosphate bond
What is the P/O ratio for succinate?
1.5
1.25
2.5
2.25
How many ATP does 1 NADH produce?
2.5
1.5
2
3
If 1 NADH yields 3 ATP and 1 FADH2 yields 2 ATP, how many ATP are produced from one molecule of glucose?
38
30
29
21
How many ATP does the Krebs cycle produce?
2
3
4
5
Which of the following is not a product of the cellular respiratory chain?
O2 production
CO2 production
H2O production
H2O2 production
Which enzyme does not have a detoxification role in the body?
Hydroperoxidase
Peroxidase
Oxidase
Catalase
Which enzyme does not participate in the cellular respiratory chain?
Phosphorylase
Peroxidase
Dehydrogenase
Catalase
Which statement about free energy is correct?
The portion of cellular energy not used is called free energy
The free energy of a system is directly proportional to its entropy
A spontaneous reaction occurs with a decrease in free energy
Entropy is lowest when the system reaches equilibrium
Which product is not a product of the citric acid cycle?
CO2
NADH
FADH2
H2O
The Q cycle occurs at which complex?
Complex I
Complex II
Complex III
Complex IV
Which substance is not a membrane-bound electron carrier?
Coenzyme Q
NADH
Cytochrome
Fe–S protein
In which stages are reducing equivalents formed?
Stages 1, 3, 4
Stages 2, 5, 7
Stages 1, 6, 8
Stages 4, 6, 8
The cellular respiratory chain belongs to which stage of catabolism?
Stage 1
Stage 2
Stage 3
Stage 4
Which process belongs to the common degradative (convergent) stage of metabolism?
Proteins degraded to amino acids
Glucose degraded to pyruvate
Beta-oxidation of fatty acids
Citric acid cycle
Regarding metabolism, which statement is correct?
Catabolism is the process of breaking down complex nutrients
The free energy of catabolism is stored entirely in phosphate form
Catabolism supplies high‑energy electrons to NAD+ and NADP+
Entropy is lowest when the system reaches equilibrium
What is intermediary metabolism?
A biosynthetic process occurring in three stages
Energy is released mostly in stage 1
The half‑life of brain triglyceride is 200 days
Acetyl‑CoA is oxidized in the citric cycle to CO2 and H2O
What is anabolism?
The process converting dietary specific macromolecules into the body’s specific macromolecules
The process converting dietary specific macromolecules into simple structural units
The hydrolysis of dietary macromolecules into specific simple units
Transfer of body macromolecular units into dietary macromolecules
In catabolic reactions, what percentage of the released energy is captured as ATP?
40%
50%
60%
70%
In catabolism, at which stage is the carbon skeleton of alpha‑keto acids formed?
Stage 1
Stage 2
Stage 3
Stages 2 and 3
Which processes are included in metabolism?
Digestion of food
Absorption of digestive products
Intermediary metabolism
All of A, B, and C
Catabolism consists of how many stages?
4 stages
2 stages
5 stages
3 stages
Which process is not part of overall metabolism?
Digestion of food
Absorption of digestive products
Intermediary metabolism
Formation of reducing sugars
Each metabolic pathway can take multiple forms; which is the most common form?
Linear pathway
Cyclic pathway
Spiral pathway
None of the above
Under aerobic conditions, the convergent product at the end of stage 2 of macromolecule degradation is
Pyruvate
Acetyl‑CoA
H2O
CO2
At which stage of catabolism is the carbon skeleton of alpha‑keto acids formed?
Stage 1
Stage 2
Stage 3
Stages 2 and 3
What is the first substrate‑level phosphorylation in glycolysis?
Formation of 3‑phosphoglycerate
Formation of ADP from AMP
Catalyzed by phosphofructokinase
Processes are irreversible
The enzyme that adds a phosphate group to fructose‑6‑phosphate in glycolysis does so for what main purpose?
This step produces ATP
To regulate glycolysis
To produce fructose‑2,6‑bisphosphate
It is phosphofructokinase‑2
In anaerobic metabolism, pyruvate is reduced to lactate. This process produces
ATP
FAD
NAD+
H2O
The enzyme that converts phosphodiacetone (dihydroxyacetone phosphate) to glyceraldehyde‑3‑phosphate belongs to which class?
Oxidoreductase
Transferase
Lyase
Isomerase
Five hundred molecules of glucose labeled with 14C are supplied to cells that produce lactate. How many molecules of ATP labeled with 14C are formed from these glucose molecules?
500
250
750
800
What is the proper sequence in aerobic respiration?
Acetyl‑CoA, electron transport, glycolysis, Krebs cycle
Acetyl‑CoA, glycolysis, Krebs cycle, electron transport
Krebs cycle, glycolysis, electron transport, Acetyl‑CoA
Glycolysis, Acetyl‑CoA, Krebs cycle, electron transport
Which principal substance in muscle stimulates cAMP formation to promote glycogen breakdown and inhibit glycogen synthesis?
Calmodulin
Insulin
Glucose
Glucagon
Which pair are the main products of the pentose phosphate pathway?
NAD+ and ribose
NADH and glucose
NAD+ and glucose
NADPH and ribose
What is the main substrate for gluconeogenesis?
Galactose
Glycerol
Mannitol
Sucrose
What is the role of glucagon?
Stimulates the citric acid cycle
Stimulates the pentose phosphate pathway
Stimulates glycogen synthesis
Stimulates gluconeogenesis
What is hexokinase?
Predominantly active in the liver
Has low affinity for glucose
Phosphorylates large amounts of glucose
Is inhibited by glucose‑6‑phosphate
How many ATP are consumed in glycolysis up to the formation of fructose‑1,6‑bisphosphate when starting from free glucose?
0
1
3
4
Which of the following sugars is non‑reducing?
Fructose
Sucrose
Glucose
Lactose
Which sugar is called milk sugar?
Galactose
Sucrose
Lactose
Fructose
Which substance is used to assess glomerular filtration function?
Heparin
Dextrin
Inulin
Insulin
Which substance is commonly used to replace blood plasma?
Inulin
Dextrin
Inulin
Lactose
What is the role of heparin?
Coagulates blood and clarifies plasma
Anticoagulant and clarifies plasma
Anticoagulant and clouds plasma
Coagulates blood and clouds plasma
Which substance is produced in muscle cells under anaerobic conditions?
ATP
Pyruvate
Lactate
Acetyl‑CoA
Under anaerobic conditions, pyruvate is converted to lactate; this process generates which molecule?
NAD+
ATP
FAD
H2O
The enzyme that converts phosphodiacetone to phosphoglyceraldehyde belongs to which class?
Oxidoreductase
Hydrolase
Lyase
Isomerase
Which substance primarily in muscle stimulates cAMP production to activate glycogen breakdown and inhibit glycogen synthesis?
Epinephrine
Glucagon
Insulin
Glucose
Which set contains only saturated fatty acids?
Oleic acid, palmitic acid, arachidonic acid
Stearic acid, palmitic acid, butyric acid
Palmitic acid, linolenic acid, stearic acid
Butyric acid, oleic acid, linolenic acid
Which of the following is a characteristic of tripalmitin?
Highly soluble in water
Abundant in liver
A complex lipid
A simple glyceride
Where are ketone bodies synthesized?
Liver
Muscle
Kidney
Pancreas
Where does β-oxidation occur?
Cytosol and mitochondria
Cytosol and other organelles
In mitochondria
All of the above
What is the composition of linolenic acid?
16 carbons and 1 double bond
18 carbons and 3 double bonds
18 carbons and 1 double bond
18 carbons and 2 double bonds
Which compound is a neutral fat?
Triglyceride
Diglyceride
Monoglyceride
Phosphoric acid
Which lipid component is abundant in LDL?
Triglyceride
Phospholipid
Free fatty acids
Cholesterol
Where is lipoprotein lipase most abundant in the body?
Vascular wall
Intestinal lumen
Liver
Pancreas
Which apolipoprotein is present in chylomicrons?
Apo A‑I
Apo B‑48
Apo B‑100
Apo A‑II
How many carbons does testosterone have?
24 carbons
21 carbons
12 carbons
19 carbons
Degradation of fatty acids to acetoacetyl‑CoA occurs primarily in which organ?
Adipose tissue
Intestine
Liver
Spleen
Where is cholesterol most abundant?
HDL
VLDL
Chylomicrons
LDL
Which is not a function of lipids?
Membrane structure
Energy storage
Transport
Carrying genetic information
Which lipoprotein is synthesized only in the small intestine?
HDL
LDL
Chylomicron
VLDL
Which lipid component is abundant in HDL?
Phospholipid
Free cholesterol
Triglyceride
Triglyceride A
Which lipid component is abundant in chylomicrons?
Phospholipid
Free fatty acids
Endogenous triglyceride
Exogenous triglyceride
Which apolipoprotein is present in both VLDL and LDL?
Apo A‑I
Apo B‑100
Apo A‑II
Apo B‑48
Cholesterol is not a precursor of which of the following?
Bile salts
Aldosterone
Corticosterone
Testosterone
Where does fatty acid synthesis primarily occur?
Liver
Adipose tissue
Outside mitochondria
In mitochondria
Which substance decreases the activity of HMG‑CoA reductase?
Insulin
Glucagon
TSH
Adrenaline
Where is Apo B‑48 abundant?
Chylomicrons
LDL
VLDL
HDL
Which enzyme functions at pH 1–2?
Trypsin
Pepsin
Trypsinogen
Pepsinogen
Which amino acid contains an imino group?
Valine
Tyrosine
Proline
Cystine
Which of the following has the nature of a protein?
Carbohydrate
Vitamin
Triglyceride
Enzyme
Which amino acid contains a sulfhydryl (−SH) group?
Cysteine
Threonine
Cystine
Histidine
What is keratin?
Globulin
Tripeptide
Fibrous protein
Globular protein
Which group consists only of heterocyclic amino acids?
Tyr, His, Pro, Lys
Trp, Tyr, His, Lys
Pro, His, Hyp, Trp
Pro, Phe, His, Trp
Which amino acid is basic?
Trp
Met
Val
Lys
Which substance gives a positive biuret reaction?
Amino acids
Triglyceride
Tetrapeptide
Monosaccharide
Thyroxine (T4) is derived from which compound?
Tyramine
Taurine
Tyrosine
Tryptoamine
Arginase acts on which substrate to produce urea?
Glutamate
Arginine
Glutamine
Ornithine
Which is a product of arginine hydrolysis in the urea cycle?
Ornithine
Citrulline
Glutamine
Aspartate
In what form does NH3 travel in the blood?
Pyruvate
Asparagine
Glutamine
Ammonia
How many grams of protein does the body need per day?
40–50 g
100–200 g
30–60 g
20–80 g
What is the oxidative decarboxylation product of tryptophan?
Serotonin
Epinephrine
Histamine
Melanin
What is the pH of the stomach?
2–3
1–3
6–6.5
4–5
What is the pH of the duodenum?
6–6.5
3–4
2–3
6–7
What is the pH of the large intestine?
5–7
1–3
6–6.5
5.5–7
Where is chymotrypsin secreted?
Liver
Spleen
Pancreas
Kidney
Blood protein increases in which situation?
Hemoconcentration
Diabetes mellitus
Hyperthyroidism
Blood loss
The product of amino acid metabolism excreted in urine of animals is in what form?
Uric acid
Glutamine
Urea
Creatinine
In heme, the two propionic side chains are attached to the porphyrin nucleus at which positions?
1, 2
3, 4
5, 6
7, 8
Which hemoglobin has valine at position 6 of the beta chain?
HbA
HbC
HbS
HbF
Which hemoglobin has lysine at position 6 of the beta chain?
HbA
HbC
HbF
HbS
Which set contains only derivatives that include Fe2+?
HbCO2, HbO2, Hb
HbO2, HbCO, Hb
MetHb, HbCO2, Hb
Hb, HbCO, HbCO2
Which group includes only chromoproteins whose prosthetic group contains a porphyrin nucleus?
Hemoglobin, cytochrome, glycoprotein
Hemoglobin, chlorophyll II, ferritin
Hemoglobin, cytochrome, chlorophyll II
Flavoprotein, cytochrome, chlorophyll II
Sickle cell disease is due to what?
A mutation in the gene encoding the gamma polypeptide chain
A mutation in the gene encoding the two beta polypeptide chains
A mutation in the gene encoding the two alpha polypeptide chains
A mutation in the gene encoding the delta polypeptide chain
On which chromosome is the gene encoding the alpha polypeptide chain located?
Chromosome 16
Chromosome 18
Chromosome 20
Chromosome 12
Which protein stores oxygen for muscle?
Hemoglobin
Albumin
Myoglobin
Collagen
Which form of hemoglobin is present mainly in the fetus?
HbA
HbB
HbC
HbF
How many polypeptide chains in total are present in the quaternary structure of a hemoglobin molecule?
1
2
4
5
Which of the following is a cause of prehepatic jaundice?
Bile duct obstruction
Hepatitis
Hemolysis
Pancreatitis
How many types of bilirubin are there?
1
2
3
4
What is the normal concentration of total bilirubin in serum?
<10 mg/dl
<1 mg/dl
<1 g/l
<1 mg/l
Which is a cause of prehepatic jaundice?
Viral hepatitis
Alcohol
Drugs (e.g., acetaminophen, isoniazid)
Hemolysis
Which is a cause of intrahepatic jaundice?
Neonatal jaundice
Common bile duct stone
Pancreatic head tumor
Viral hepatitis
Which is a cause of posthepatic jaundice?
Neonatal jaundice
Ascaris invading the bile duct
Hemolysis
Cirrhosis
A direct/total bilirubin ratio (conjugated/total) >80% is seen in which condition?
Hemolysis
Pancreatic head tumor
Obstruction
Drug-induced hepatitis
A direct/total bilirubin ratio (conjugated/total) <25% is seen in which condition?
Hemolysis
Neonatal jaundice
Obstruction
Viral hepatitis
In DNA, which nitrogenous base pair is connected by three hydrogen bonds?
Adenine and guanine
Adenine and thymine
Cytosine and guanine
Cytosine and adenine
Which of the following is not a purine base?
Guanine
Caffeine
Adenine
Cytosine
Which of the following is not a pyrimidine base?
Thymine
Cytosine
Uracil
Guanine
Which nitrogenous base contains a methyl group (-CH3) in its structure?
Guanine
Thymine
Uracil
Cytosine
Which of the following is a nucleotide?
Guanosine
Thymidine
Cyclic AMP
Deoxyadenosine
