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Tissue and tissue culture

Total questions: 117

Worksheet time: 1hrs 4mins

Name
Class
Date
1.

) In plant tissue culture technique surface sterilization of explant is made by…………

a)

Hydrochloric acid

b)

Sulphuric acid

c)

Mercury chloride

d)

Nitric acid

2.

The two strand of double helix model of DNA are holdind together by……..

a)

Sugar & nitrogen base

b)

Sugar &phosphate group

c)

Phosphate &nitrogen base

d)

Phosphate &phosphate group

3.

..……….proposed definite model of molecular structure

a)

Watson

b)

Crick

c)

Watson &crick

d)

Daniel

4.

Agrobacterium cell consist of ………..as independent replication circular DNA

a)

Cosmid

b)

Plasmid

c)

Ti-Plasmid

d)

L-Plasmid

5.

Restriction Endonucleases are ……………enzyme

a)

Bacterial

b)

Viral

c)

Fungal

d)

Algal

6.

The term ‘Totipotency’ was given by _____________.

a)

T.H. Morgan

b)

Haberlandt

c)

Schwann

d)

De Robertis

7.

In PCR reaction .....enyme is used.

a)

Taq polymerase

b)

Reductase

c)

Phosphatse

d)

Dehydrogenase

8.

The method of producing thousands of plants through tissue culture is called

a)

somatic hybridiastion

b)

totipotency

c)

micro-propagation

9.

The capacity to generate a whole plant from any cell/explant

a)

micropropagation

b)

Tissue culture

c)

Totipotency

d)

somatic hybridisation

10.

In plant tissue culture, what is the term ORGANOGENESIS means?

a)

Formation of callus culture

b)

Formation of root & shoot from callus culture

c)

Genesis of organ

d)

None of the above

11.

Ability of a plant to endure extreme conditions by changing growth and development of plant organs

a)

Petiole

b)

Micropropagation

c)

Pathogenic

d)

Plasticity

12.

Plant tissue culture is a practice used to propagate plants under sterile conditions, often to produce ________ of a plant.

a)

DNA

b)

Bacteria

c)

Cloning

d)

Chromosome

13.

What is the name of naturally occurring Auxin in plant?

a)

1-napthaleneacetic acid (NAA)

b)

2,4-Dichlorophenoxyacetic acid (2,4-D)

c)

Indole 3-acetic acid (IAA)

d)

1-napthoxyacetic acid (NOA)

14.

In Plant Tissue Culture, Totipotency means

a)

Flowering in culture medium

b)

Development of Organ from Tissue under medium

c)

Development of fruit

d)

None

15.

Micropropagation is widely used in ________ and in floriculture.

a)

Wilderness

b)

Deforestation

c)

Sustainability

d)

Forestry

16.

Plant tissue culture is a practice used to propagate plants under sterile conditions, often to produce ________ of a plant.

a)

DNA

b)

Bacteria

c)

Cloning

d)

Chromosome

17.

In palnt tissue Cultue, Which part shows maximum totipotency?

a)

Xylem

b)

Phloem

c)

Meristem

d)

Stem

18.

In Plant Tissue Culture, the callus is grown in plantlet by altering the concentration of _______

a)

Amino acid

b)

Glucose

c)

Hormone

d)

Al of the above

19.

Factors Associated in Somatic embryogenesis

a)

Light intensities

b)

particular wavelengths

c)

photoperiods

d)

gamma ray

20.

BASIC MEDIA COMPONENTS FOR PLANT CELL CULTURE IN MS MEDIA FOR GLYCINE

a)

2mg/l

b)

12mg/ml

c)

7mg/ml

d)

190mg/ml

21.

Disadvantages of Micropropagation

a)

expensive and time consuming

b)

expensive

c)

time consuming

22.

How to Propagate?

a)

Induction of direct regeneration of shoots from organ cultures.

b)

Induction of direct regeneration of roots from organ cultures.

c)

Induction of direct regeneration of stomata from organ cultures.

d)

Induction of direct regeneration of xilem from organ cultures.

23.

Plant parts (cell, tissue, organ) used in tissue culture called as the

a)

import

b)

implant

c)

explant

d)

export

24.

Scientist known as the father of tissue culture

a)

Scleiden

b)

Murashige

c)

Gottlieb Haberlandt

d)

Simon

25.

Stages of Callus Culture

a)

induction > proliferation > morphogenesis

b)

proliferation > induction > morphogenesis

c)

morphogenesis > induction > proliferation > mutation

d)

induction > mutation > proliferation > morphogenesis

26.

What does it means by micropropagation produces genetically identical plants. The production of

a)

true to types plants

b)

somaclones

c)

somaclonal variation

d)

false to types plants

27.

Tissue culture technique is an efficient tool used in plant breeding for the following reasons EXCEPT

a)

mass propagation of specific clones.

b)

useful for plant reproduced by asexual.

c)

production of high yield diseased plants.

d)

production of healthy or diseased-free plants.

28.

Healthy plants can be grown from diseased plant by

a)

meristem culture

b)

leaf culture

c)

stem culture

d)

root culture

29.

Which of the following statements are the APPLICATIONS of plant tissue culture technique.


I Micropropagation

II Genetic improvement

III Germplasm preservation

IV Production of synthetic seed

a)

I and II

b)

II and III

c)

I, II and III

d)

All of the above.

30.

Apical meristems can be found in

a)

The leaves

b)

The tips of the shoots and roots

c)

The xylem cells

d)

The epidermal cells

31.

Apical meristems can be found in

a)

The leaves

b)

The tips of the shoots and roots

c)

The xylem cells

d)

The epidermal cells

32.

Specific type of plant tissue that transports water and minerals from the roots, through the stems, to leaves

a)

vascular plant

b)

nonvascular plant

c)

xylem

d)

phloem

33.

Specific type of plant tissue that transports food (sugar) made in the leaves to the entire plant

a)

vascular plant

b)

nonvascular plant

c)

xylem

d)

phloem

34.

Sieve tubes and companion cells are present in

a)

xylem

b)

phloem

c)

cambium

d)

cork

35.

Other than transporting water, what is a function of xylem?

a)

making food

b)

transporting food

c)

helping support the plant

d)

catching light

36.

A tissue whose cells are capable of dividing and re-dividing is called

a)

complex tissue

b)

permanent tissue

c)

connective tissue

d)

meristematic tissue

37.

Xylem and phloem are examples of

a)

epidermal tissue

b)

connective tissue

c)

protective tissue

d)

vascular tissue

38.

The size of the stem increases in the width due to

a)

apical

b)

intercalary

c)

lateral

d)

vascular bundle

39.

Packaging tissue

a)

parenchyma

b)

collenchyma

c)

xylem

d)

phloem

40.

The nuclei of meristematic cells are

a)

small

b)

large

c)

medium sized

d)

no nucleus

41.

Which of the following is an undifferentiated embryonic tissue found in the active growth regions of plants such as tips of roots and stems?

a)

Parenchyma tissue

b)

Collenchyma tissue

c)

Meristematic tissue

d)

Permanent tissue

42.
What is a tissue
a)
A group of cells that share a common goal
b)
a tissue that has a function
c)
A group of cells that share a common function, structure or both
d)
A group of cells
43.

What type of ground tissue is this?

a)

Parenchyma

b)

Schlerenchyma

c)

Collenchyma

44.
What type of cells give the edge of this celery flexible support?
a)
Parenchyma
b)
Sclerenchyma
c)
Collenchyma
45.

What type of ground tissue is this?

a)

Parenchyma

b)

Collenchyma

c)

Schlerenchyma

46.
which tissue provide support and mechanical strength to the plant?
a)
parenchyma tissue
b)
sclerenchyma tissue
c)
xylem tissue
d)
collenchyma tisssue
47.
What type of cells are shown here? Note:  These are alive at maturity.
a)
Parenchyma
b)
Sclerenchyma
c)
Collenchyma
48.
which of this tissues support the young plants and woody plants?
a)
collenchyma tissue
b)
parenchyma tissue
c)
sclerenchyma tissue
d)
xylem tissue
49.

Both a plant and animal cells have a cell wall

a)

True

b)

False

50.

Do meristematic tissues have vacuoles present in them?

a)

Yes

b)

No

51.

It helps water Hyacinth to float on water. What type of parenchyma tissue is it?

a)

Chlorenchyma

b)

collenchyma

c)

Aerenchyma

d)

schlerenchyma

52.

I am a kind of parenchymatous cell, and I perform photosynthesis. Who am I?

a)

Collenchyma

b)

Aerenchyma

c)

Chlorenchyma

d)

Sclerenchyma

53.

There is more Intercellular space found in this tissue

a)

Collenchyma

b)

Sclerenchyma

c)

Parenchyma

d)

Epidermis

54.
This is the type of meristem found at the tips of roots and stems that causes primary growth?
a)
Lateral meristem
b)
Epidermis
c)
Intercalary meristem
d)
Apical meristem
55.
Sclerenchyma cells are ____________ at maturity
a)
Living
b)
Non-living
56.

Which is not a function of epidermis?

a)

protection from adverse condition

b)

gaseous exchange

c)

conduction of water

d)

transpiration

57.

Which muscles act involuntarily?

a)

striated muscles

b)

cardiac muscles

c)

skeletal muscles

d)

All the above

58.

The muscular tissue which function throughout the life

continuously without fatigue is

a)

skeletal muscle

b)

cardiac muscle

c)

smooth muscle

d)

voluntary muscle

59.

While doing work and running, you move your organs such as hands, legs, etc., which among the following is correct?

a)

smooth muscles contract and pull the ligament to move the

bones

b)

smooth muscles contract and pull the tendons to move the

bones

c)

skeletal muscles contract and pull the ligament to move the

bones

d)

skeletal muscles contract and pull the tendon to move the

bones

60.

Voluntary muscles are found in

a)

alimentary canal

b)

limbs

c)

iris of the eye

d)

bronchi of lungs

61.

A person met with an accident in which two long bones of

hand were dislocated. Which among the following may be the possible reason?

a)

tendon break

b)

break of skeletal muscles tissue

c)

ligament break

d)

areolar tissue break

62.

Bone matrix is rich in

a)

fluoride and calcium

b)

calcium and phosphorus

c)

phosphorus and potassium

d)

calcium and potassium

63.

Which of the following helps in repair of tissue and fills up the space inside the organ?

a)

tendon

b)

adipose tissue

c)

areolar tissue

d)

cartilage

64.

Bone matrix is rich in

a)

fluoride and calcium

b)

calcium and phosphorus

c)

phosphorus and potassium

d)

calcium and potassium

65.

Fats are stored in human body as

a)

cuboidal epithelium

b)

adipose tissue

c)

bones

d)

cartilage

66.

Cartilage is not found in

a)

nose

b)

ear

c)

kidney

d)

larynx

67.

These cells are small and flat

a)

Cuboidal

b)

Columnar

c)

Squamous

d)

Pseudostratified

68.

Which of the following is NOT a function of epithelial tissues?

a)

covering exposed surfaces

b)

lining internal passageways

c)

producing glandular secretions

d)

contracting to produce movement

69.

Name the tissue

a)

Pseudostratified

b)

Simple columnar

c)

Transitional

d)

Stratified Squamous

70.

Name the type of epithelium

a)

simple cuboidal

b)

simple squamous

c)

simple columnar

d)

stratified squamous

71.
Used in places where diffusion needs to occur, such as blood vessels and air sacs in the lungs
a)
Cuboidal
b)
Columnar
c)
Simple Squamous
d)
Stratified Squamous
72.
Lines ducts and glands
a)
Columnar
b)
Cuboidal
c)
Simple Squamous
d)
Transitional
73.
Absorbs nutrients in the digestive tract
a)
Columnar
b)
Cuboidal
c)
Simple Squamous
d)
Pseudostratified
74.
Identify:
a)
Pseudostratified
b)
Simple cuboidal
c)
Transitional
d)
Simple Squamous
75.

Which muscle type can contract rapidly with great force, BUT tires easily and must rest after short periods of activity.

a)

skeletal

b)

smooth

c)

cardiac

76.
Nervous tissue
a)
Tissue that is responsible for lining, covering, and glandular tissue
b)
Tissue responsible for protecting, supporting, and binding together other body tissues
c)
Tissues that contracts and shortens to produce movement
d)
Tissue that receives and conducts electrochemical impulses from one part of the body to another
77.
Connective tissue is
a)
The tissue that protects, supports, and binds together other body tissue
b)
Tissue that is the lining, covering, and glandular tissue of the body
c)
Tissue that contracts and shortens to produce movement
78.

Where are dogs worshipped? They have a festival!

a)

India

b)

China

c)

Nepal

79.

People of the Ancient Egypt considered ___________ as sacred and as an important deity because they kept an eye on storms, chaos, and darkness.

a)

cats

b)

dogs

c)

pigs

80.
Where is epithelial tissue found?
a)
glands
b)
covering body surfaces
c)
lines organs
d)
all of the above
81.
The blood stain most likely belongs to 
a)
Bob
b)
Sue
c)
John
d)
Lisa
82.
Artificially changing the genetic material of a cell.
a)
Amino Acid
b)
Nucleic Acid
c)
Phenotype 
d)
Genetic Engineering
83.
The total hereditary material of a cell
a)
Genome
b)
Gene
c)
Genetic Engineering
d)
Hybrid
84.
using living things or parts of living things to produce a product or solve a problem
a)
biotechnology
b)
tissue culturing
c)
bioremediation
d)
transgenic
85.
North Carolina's #1 Biotech Industry is
a)
Biomedical Research
b)
Environmental Biotechnology
c)
Making Clones
d)
Agriculture
86.
Using microbes like bacteria to remove harmful toxins from the environment is called
a)
bioremediation
b)
transgenic
c)
tissue culturing
d)
gene therapy
87.
Using a virus to enter a cell and replace defective genes with healthy genes is called
a)
tissue culturing
b)
bioremediation
c)
cloning
d)
gene therapy
88.
All of the following are advantages of GMO's except
a)
pest resistant crops
b)
more nutritional foods
c)
unknown long term health effects
d)
creating more human insulin
89.
Using biotechnology to change the genetic makeup of an organism is called
a)
Tissue Culturing
b)
Biotechnology
c)
Cloning
d)
Genetic Engineering
90.

For culturing, plasma from the adult chicken is preferred to mammalian plasma because

a)

it forms a clear and solid coagulum even after dilution

b)

it is too opaque

c)

it doesn't produce solid clots

d)

it forms a semi solid coagulum

91.

Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is

a)

150-300

b)

280-360

c)

300-325

d)

360-400

92.

Accumulation of lactate leads to

a)

increase in pH

b)

no change in pH

c)

reduction in the pH of culture hence loss of cell viability

d)

no loss of cell viability

93.

What is animal tissue culture?

a)

Growth and maintenance of animal cells

b)

Growth and selling of animal cells

c)

Only maintenance of animal cells

d)

All

94.

Culture freshly prepared from isolated tissue is known as _____

a)

Organ culture

b)

Primary culture

c)

Cell line

d)

Histotypic culture

95.

______________is the primary equipment required for animal tissue culture laboratories.

a)

Glasswares

b)

Laminar flow

c)

Sterilizers

d)

All

96.

The material used in culture techniques is important because the surface of the vessel serves as a substrate for cells to grow.

a)

True

b)

False

97.

_________________is one of the limitation of animal tissue culture.

a)

Disposal of biohazards is not easy

b)

Cultured cells are not easy to store

c)

Both

d)

None

98.

The foundation for the development of cell culture technique was laid by

a)

Roux

b)

Arnold

c)

Ross

d)

Harrison

99.

Who is regarded as the father of tissue culture

a)

Harrison

b)

Arnold

c)

Ross

d)

Roux

100.

The limited replicative capacity of human cells in culture is called

a)

Brownian effect

b)

Contact inhibition

c)

None of the above

d)

Hayflick effect

101.

HAT medium used for the selection of fused hybrid cells in hybridoma culture was introduced by

a)

Littlefield and Miller

b)

Kohler and Milstein

c)

Frish and Jentoft

d)

Eagle and Karl

102.

Which of the following is HGPRT+ and survives in HAT medium

a)

B cells

b)

Myeloma cells

c)

Hybrid cells

d)

Both a and c

103.

Use of trypsin to free cells from tissue matrix for cell culture was described by

a)

Jolly and Koch

b)

Beebe and Ewing

c)

Sims and Stillman

d)

Gottieb Maberland

104.

Vero cell line is obtained from Kidney cells of

a)

African green monkey

b)

Chinese hamster

c)

Calf

d)

Swine

105.

Which of the following is easy and rapid method to interpret viability of cells in culture system

a)

Trypan blue dye exclusion

b)

Neutral red assay

c)

Fluorescein dye assay

d)

All of the following

106.

Which of the following virus is used for cell fusion

a)

Sendai virus

b)

Herpes virus

c)

Myxovirus(

d)

All of the following

107.

Which of the following is most commonly used cell fusing agent

a)

PEG

b)

NaNO3

c)

Sendai virus

d)

Polyvinyl alcohal

108.

The ratio of CO2 : O2 used in cell culture system should be

a)

1:5

b)

1:13

c)

1:19

d)

1:25

109.

Which cell line is used for production of recombinant sex hormones

a)

BHK cell line

b)

Vero cell line

c)

Hela cell line

d)

CHO cell line

110.

Which of the following is the oldest cell line

a)

Hela cell line

b)

Vero cell line

c)

CHO cell line

d)

BHK cell line

111.

Optimum pH required for the growth of mammalian cells is

a)

5.3-7.0

b)

6.5-7.0

c)

7.2-7.4

d)

8.1-8.9

112.

For culture of avian cells the optimum temperature requirement is

a)

37 ̊C

b)

40 ̊C

c)

42 ̊C

d)

35 ̊C

113.

The optimum temperature of any cell or organ is called

a)

Critical temperature

b)

Threshold temperature

c)

Ceiling temperature

d)

None of the above

114.

Which of the following is the structural fibre in cell culture system

a)

Collagen

b)

Elastin

c)

Fibronectin

d)

Both a and b

115.

Which of the following is true regarding animal cell culture technique

a)

Lactic acid is source of carbon

b)

Cells have high requirement of L-glutamine

c)

Cholin is necessary for cell adhesion and cytoskeleton

d)

All of the following

116.

Cell culture technique became simpler only after advent of

a)

Antibiotics

b)

Trypsin

c)

Cell culture media

d)

All of the following

117.

Cells used in feeder layer

a)

Should have ability to divide

b)

Have ability to metabolize

c)

These properties are obtained by exposing cells to irradiation

d)

All of the above

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