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WorksheetsBacteriology
Total questions: 96
Worksheet time: 48mins
Name
Class
Date
1.
Which of the following is not taken as a specimen to diagnose staphylococcal infection?
a)
Pus: Suppurative lesion
b)
Blood: meningitis
c)
Sputum: respiratory infection
d)
Faeces/ Vomit: Food poisoning
2.
What is 'Z' in the following statement?
Treponema pallidum is the causative organism of Z.
a)
Pyogenic meningitis
b)
Chancroid
c)
Syphilis
d)
Vibrio cholerae
3.
Which pathogen is Gram-negative, short, rigid, curved rods that are actively motile by a polar flagellum?
HINT: A causative organism of Cholera
a)
N. meningitidis and N. gonorrhoeae
b)
Pseudomonas aeruginosa
c)
Vibrio cholerae
d)
H. influenzae
4.
Which of the following CORRECTLY denotes the following?
Gram-negative bacteria which are highly infectious, parasites of the human intestine, and cause bacillary dysentery in man.
a)
Shigella
b)
E.coli
c)
Enterobacter
d)
Proteus
5.
Identify the following bacteria
HINT: Motile, Gram-negative bacilli, characterized by swarming growth on agar.
a)
E.coli
b)
Enterobacter
c)
Proteus
d)
None of the above
6.
What is Xin the following statement?
Esch. coli is a parasite inhabiting only the human or animal intestine. Many strains of Esch. coli contain X.
a)
Klebsiella
b)
Flagella
c)
Proteus
d)
Shigella
7.
Which bacteria show club-shaped swellings?
HINT: One of its members causes diphtheria in humans
a)
Pseudomonas
b)
Corynebacterium
c)
Vibrio
d)
Shigella
8.
What is the other name of Pneumococcus?
a)
Enterobacter pneumoniae
b)
Staphylococcus pneumoniae
c)
Streptococcus pneumoniae
d)
None of the above
9.
Which of the following is the most Important pathogen causing pneumonia and otitis media in children?
a)
Pneumococci
b)
Micrococci
c)
Corynebacteria
d)
Klebsiella
10.
What is the route of injection in the Mantoux test?
a)
Subcutaneous
b)
intradermal
c)
Intravenous
d)
Intramuscular
11.
Which of the following is a rapid method in the diagnosis of tuberculosis?
a)
Widal test
b)
PCR
c)
VDRL
d)
ELISA
12.
Which of the following is TRUE about the growth of M.tuberculosis on culture media?
Colonies appear in 3-5 days.
colonies appear in less than 1 week
colonies appear in 2-8 weeks
colonies grow slowly
a)
1, 3 and 4
b)
l and 2
c)
l and 3
d)
3 and 4
13.
Which of the following selective culture media is used for the detection of M. tuberculosis?
a)
TCBS
b)
Taurocholate media
c)
Loeffler's serum slope media
d)
Lowenstein Jensen Media
14.
Which of the following is the most sensitive (10 bacilli per ml) for the detection of M. tuberculosis?
a)
Gram staining
b)
Acid fast staining
c)
Culture
d)
Biochemical reaction
15.
What is 'B" in the following statement?
In the Petroff method of specimen concentration, the deposit is neutralized by adding B.
a)
8% hydrochloric acid
b)
4% hydrochloric acid
c)
4% Sodium hydroxide
d)
8% Sodium hydroxide
16.
What is ‘A’ in the following sentence?
Under the Petroff method of specimen concentration sputum is mixed with an equal volume of ‘A
a)
8% hydrochloric acid
b)
4% hydrochloric acid
c)
4% Sodium hydroxide
d)
8% Sodium hydroxide
17.
What is the reason of sample concentration in the detection of M. tuberculosis?
a)
To achieve Homogenization of specimen
b)
To concentrate the bacilli in a small volume without inactivation
c)
To kill other bacteria present in the specimen
d)
All of the above
18.
Which of the following method is used to concentrate the specimen of sputum for TB detection?
a)
Petroff method
b)
Centrifugation
c)
Flotation
d)
Sedimentation
19.
What do you say about the following statement?
Purulent part of the sputum is used for making a smear for detection of tubercle bacilli in Ziehl Neelsen staining.
a)
Always false
b)
Sometimes true
c)
True In some conditions
d)
Always true
20.
What is the minimum number of fields that must be examined before a negative report of tuberculosis is prepared?
a)
3.0
b)
30.0
c)
300.0
d)
3000.0
21.
What form does the CSF specimen take on standing in case of tuberculous meningitis?
a)
Net
b)
Ant
c)
Spider web clot
d)
Spider
22.
Which specimen is taken for detection of pulmonary tuberculosis in children?
a)
Gastric washing
b)
Saliva
c)
Nasophrangeal swab
d)
Tissue biopsy
23.
Which specimen is taken for the detection of pulmonary tuberculosis?
a)
Aspirated fluid
b)
Tissue biopsy
c)
Sputum
d)
CSF
24.
Where does M. tuberculosis cause tuberculosis?
a)
Lungs
b)
Kidney
c)
Bone
d)
Meninges
e)
All of the above
25.
What is the most common route of infection of Mycobacterium tuberculosis in humans?
a)
Inhalation
b)
Ingestion
c)
Contact
d)
Inoculation
26.
What is the color of M.tuberculosis bacilli and other cells respectively on Ziehl Neelsen staining?
a)
Blue, Red
b)
Purple, Pink
c)
Red, Blue
d)
Pink, purple
27.
What is the mode of transmission of M. tuberculosis?
a)
Vertical transmission
b)
Droplet transmission
c)
Faecoral transmission
d)
Vector borne disease
28.
Which of the following can't be used to detect M.tuberculosis?
a)
Ziel Neelsen stain
b)
Auramine Rhodamine stain
c)
Acridine orange stain
d)
Gram stain
29.
Which of the following is NOT true about Mycobacteria?
a)
Slender bacilli
b)
Aerobic, non motile
c)
Fast growing organism
d)
Non sporing And non capsulated
30.
Which of the following species of mycobacterium is responsible for leprosy in humans?
a)
M. bovis
b)
M. tuberculosis
c)
M. leprae
d)
M. africanum
31.
Which of the following species of mycobacterium is responsible for tuberculosis in humans?
a)
M. bovis
b)
M. tuberculosis
c)
M. leprae
d)
Both A and B
32.
Which animal is the main reservoir of M. tuberculosis?
a)
Cats
b)
Cattle
c)
Humans
d)
Birds
33.
Which stain is used to visualize mycobacteria?
a)
Gram stain
b)
Acid fast stain
c)
Giemsa Stain
d)
Albert stain
34.
Which of the following is TRUE about mycobacteria?
a)
Gram negative cocci
b)
Acid fast bacilli
c)
Gram positive cocci
d)
All of the above
35.
What is the characteristic colony feature of H. influenzae on culture?
a)
Swarming growth
b)
Hemolysis of RBCs
c)
Satellitismm around S. aureus
d)
Darting motility
36.
Which of the following culture media is NOT used to grow H.influenzae?
a)
Chocolate agar
b)
Blood agar
c)
Nutrient agar
d)
None of the above
37.
What can be used to diagnose H.influenzae?
a)
Gram staining
b)
Immmunofluorescence
c)
Latex agglutination
d)
All of the above
38.
What do you say about the following statement?
H. influenzae is very sensitive to temperatures. So, clinical specimens should never be refrigerated
a)
Totally true
b)
Partially true
c)
Totally false
d)
Sometimes true
39.
Which of the following specimen is NOT used for the diagnosis of H.influenzae?
a)
Cerebrospinal fluid (CSF)
b)
Blood
c)
Throat swab
d)
Sputum
e)
None of these
40.
Which of the following is NOT caused by H.influenzae?
a)
Meningitis
b)
Dysentry
c)
Pneumonia and bronchitis
d)
Otitis media
41.
Which of the following statement is TRUE?
H. influenzae are often called coccobacilli
This is because of pleomorphism
a)
1.0
b)
2.0
c)
l and 2
d)
None
42.
Which of the following is a feature of H. influenzae?
a)
Gram positive cocci
b)
Motile
c)
Pleomorphism
d)
All of the above
43.
Which of the following is the causative organism of chancroid?
a)
H. influenzae
b)
T. pallidum
c)
S. sonnei
d)
H. ducreyi
44.
What type of colonies are formed by Shigella on MacConkey's agar?
a)
Pink colonies
b)
Colorless or pale
c)
Red colony
d)
Green colony
45.
Which medium is used for the selective enrichment of Shigella?
a)
Mannitol salt agar
b)
Selenite F broth
c)
Loeffler's serum slope
d)
TCBS
46.
Which media is used for growing shigella?
a)
MacConkey Agar
b)
Deoxycholate Citrate Agar (DCA)
c)
Xylose Lysine Desoxycholate (XLD) Agar
d)
All of the above
47.
Which of the following is NOT shown on microscopic examination in the fecal sample containing shigella?
a)
Plenty of pus cells
b)
RBCs & a dead background
c)
Motile bacteria
d)
None of the above
48.
What is shown on macroscopic examination of a sample of Shigella?
a)
Scanty faeces
b)
Mucus
c)
Bright red due to blood
d)
All of the above
49.
Which transport media is used for the specimen in case of shigella?
a)
Buffered glycerol water
b)
Cary's blair media
c)
Amie's media
d)
Stuart's Media
e)
Both A and B
50.
Which of the following specimen is used for diagnosis of shigella?
a)
Urine sample
b)
Feces sample
c)
Nasopharyngeal swab
d)
CSF
51.
What are the clinical features of Shigella infection?
Abdominal cramps
Watery diarrhea with blood and Mucus
Dehydration
Whitening of skin over hands and feet
a)
1 and 4
b)
1, 3 and 4
c)
1, 2 and 3
d)
2,3 and 4
52.
What is the incubation period for shigella bacilli?
a)
1-2 days
b)
3-4 days
c)
1 week
d)
15 days
53.
What is the mode of transmission for shigella?
a)
Droplet transmission
b)
Faeco-oral transmission
c)
Vector borne transmission
d)
Air borne transmission
54.
Whats not true about shigella?
a)
Short rod shaped bacilli
b)
Non-spore-forming
c)
Non-motile
d)
Capsulated
55.
Which of the following is responsible for bacillary dysentery?
a)
Klebsiella
b)
Shigella spp.
c)
Pseudomonas aeruginosa
d)
E. coli.
56.
Which of the following best describes Shigella?
a)
Gram positive cocci
b)
Gram positive bacilli
c)
Gram negative cocci
d)
Gram negative bacilli
57.
Which of the following is TRUE about Salmonella typhi?
a)
Catalase positive
b)
Oxidase negative
c)
MR positive
d)
All of the above
58.
On what principle is the Widal test based?
a)
Antigen is detection using neutralization assay
b)
Antigen binds to its soluble antibody and forms visible precipitates
c)
Antigen binds to the RBC and form agglutination
d)
None of the above
59.
Which of the following serological test is used to diagnose typhoid?
a)
QBC
b)
VDRL
c)
Widal test
d)
All of the above
60.
What are the characteristics of colonies of salmonella on MacConkey’'s agar or DCA?
a)
Pink and non lactose fermenting
b)
Pale and non-lactose fermenting
c)
Pink and lactose fermenting
d)
Pale and lactose fermenting
61.
Which of the following is least preferred for the detection of Salmonella typhi?
a)
Blood culture
b)
Widal test
c)
Urine culture
d)
Feaces Culture
62.
What is the color of S. typhi colonies on Wilson-Blair medium?
a)
Brown
b)
Black
c)
Red
d)
Yellow
63.
Which of the following selective enrichment media is used for diagnosis of salmonella in faeces?
a)
Loeffler's serum slope media
b)
LJ media
c)
TCBS
d)
Selenite and tetrathionate broth
64.
Which of the following is NOT used to culture the fecal sample in the diagnosis of Salmonella typhi?
a)
MacConkey’s agar
b)
DCA
c)
XLD
d)
Mannitol salt agar
65.
In the diagnosis of typhoid, when do you expect the faeces culture to be positive?
a)
As soon as illness start
b)
Ist week
c)
Before second week of illness
d)
After the second week of illness.
66.
A. Taurocholate broth is inhibitory to Gram positive bacteria
B. Therefore, Gram-negative bacilli are selected out, hence used for blood culture in typhoid fever.
a)
Part A is right but part B is wrong
b)
Part B is right but part A is wrong
c)
Both are right
d)
Both are wrong
67.
Blood culture for the detection of typhoid is most accurate in
a)
Ist week
b)
2nd week
c)
3rd week
d)
After 3 weeks
68.
Glucose broth and taurocholate broth are subcultured on--------------
a)
Blood agar
b)
Mac Conkey's media
c)
Blood agar and Mac Conkey's media respectively
d)
Mac Conkey's media and Blood agar respectively
69.
Blood for salmonella detection is cultured on
a)
Glucose broth
b)
Taurocholate broth
c)
Both A and B
d)
Peptone water
70.
A. Blood culture in typhoid fever will be most effective after 3rd week for diagnosis of typhoid
B. This is because salmonella has an incubation of 21 days.
a)
Part A is right but part B is wrong
b)
Part B is right but part A is wrong
c)
Both are right
d)
Both are wrong
71.
A. Salmonellae grow on ordinary culture media at an optimum temperature of 37 degree celsius
B. Salmonellae produce circular colonies
a)
Part A is right but part B is wrong
b)
Part B is right but part A is wrong
c)
Both are right
d)
Both are wrong
72.
The specimen used for detection of the Salmonella include all EXCEPT
a)
Blood
b)
Pus
c)
Urine
d)
Faeces
73.
Which of the following is NOT caused by Salmonella?
a)
Enteric fever
b)
Gastroenteritis
c)
Septicemia
d)
Meningitis
74.
Which of the following species of Salmonella is the causative agent of typhoid?
a)
Salmonella enterica
b)
Salmonella bongori.
c)
Salmonella typhi
d)
All of them
75.
Which of the following media is NOT used to culture S.pneumoniae?
a)
Blood agar
b)
Nutrient broth
c)
Glucose broth
d)
All of them
76.
CSF specimen should be refrigerated in case of delay in processing
a)
True
b)
False
c)
Maybe
d)
Depending on the operator
77.
Which of the specimen is used for the detection of pneumococci?
a)
Sputum
b)
Cerebrospinal fluid (CSF)
c)
Pleural exudate
d)
All of the above
78.
What is true about S.pneumoniae?
a)
Flame shaped cocci
b)
Capsulated
c)
Non-motile and non-sporing.
d)
All of the above
79.
What is the morphology of S.pyrogen and S. pneumoniae respectively?
a)
Grape like Cluster, Diplococci
b)
Diplococci, Single cocci
c)
Chain, Diplococci
d)
Singly, Chain
80.
Which of the following is TRUE about S. pneumoniae?
a)
Gram positive cocci
b)
Gram positive bacilli
c)
Gram negative bacilli
d)
Gram negative cocci
81.
Which of the following is not useful to diagnose streptococcus?
a)
Throat Swab
b)
Exudate
c)
Pus
d)
CSF
82.
What is the selective media used for streptococci?
a)
Mannitol salt agar
b)
Aminoglycoside rich media
c)
TCBS media
d)
Any of the above
83.
Which of the following transport medium is used for streptococcal infections?
a)
Amie's
b)
Cary Blair's media
c)
Pike's media
d)
Stuart's media
84.
Which of the following is true about streptococcus?
a)
Gram positive
b)
Rod shaped
c)
Arranged in grape like clusters
d)
Motile
85.
Which of the following is NOT true about micrococci?
a)
Coagulase positive
b)
Catalase positive
c)
Gram positive
d)
Break down carbohydrate oxidatively
86.
What is the color of the micrococci colonies on ordinary culture media?
a)
Yellow
b)
White
c)
Black
d)
Green
87.
What is the appearance of Micrococci on gram staining?
a)
Grape like Cluster form
b)
Chain form
c)
Irregular clusters
d)
Any of the above
88.
Which of the following statement is TRUE?
a)
Micrococci are gram negative cocci
b)
Micrococci are gram negative bacilli
c)
Micrococci are gram positive bacilli
d)
Micrococci are gram positive cocci
89.
Which of the following biochemical test result is NOT true for staphylococcus?
a)
Catalase positive
b)
Oxidase negative
c)
Ferment carbohydrate
d)
Mannitol negative
90.
Which of the following test is used to distinguish streptococcus and staphylococcus?
a)
Catalase
b)
Oxidase
c)
Gram staining
d)
All of the above
91.
What is the color of colonies of staphylococcus on MacConkey's medium?
a)
Pink due to non-lactose fermentation
b)
Pale due to non-lactose fermentation
c)
Pink due to lactose fermentation
d)
Pale due to lactose fermentation
92.
Which of the following culture media is used for staphylococcus?
a)
Nutrient agar
b)
Blood agar
c)
Mannitol salt agar
d)
All of the above
93.
Which of the following selective media is used for Staphylococcus aureus?
a)
TCBS
b)
Tryptic Soy Agar
c)
Mannitol salt agar
d)
LJ media
94.
Which type of hemolysis is shown by Staphylococcus culture on blood agar?
a)
Alpha hemolysis
b)
Beta hemolysis
c)
Gamma hemolysis
d)
All of these
95.
What of the following is NOT the characteristic of Staphylococcus aureus on culture media?
a)
Circular, smooth, convex, opaque colonies
b)
Golden-yellow pigment
c)
No haemolysis on sheep blood agar
d)
None of the above
96.
What is the appearance of staphylococcus appedrs on gram staining?
a)
Purple color cocci in cluster
b)
Pink color cocci in chain
c)
Purple color bacilli in cluster
d)
Purple color bacilli in cluster
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