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BIOCHEMmidterms

Total questions: 65

Worksheet time: 50mins

Name
Class
Date
1.

what enzyme group is responsible for oxidation-reduction reactions

(a)  

2.

what enzyme group is responsible for transfer of functional groups

(a)  

3.

what enzyme group is responsible for hydrolysis reactions

(a)  

4.

what enzyme group is responsible for addition to double bonds or the reverse of that reaction

(a)  

5.

what enzyme group is responsible for isomerization reactions

(a)  

6.

what enzyme group is responsible for formation of bonds with ATP cleavage

(a)  

7.

what enzyme catalyzed formation of ester linkages

(a)  

8.

what enzyme catalyzed removal of carboxyl groups from compounds

(a)  

9.

what enzyme catalyzed hydrolysis of peptide linkages

(a)  

10.

what enzyme catalyzed hydrolysis of phosphate ester linkages

(a)  

11.

presence of this enzyme may indicate prostate cancer

(a)  

12.

presence of this enzyme may indicate liver or bone disease

(a)  

13.

presence of this enzyme may indicate diseases of the pancreas

(a)  

14.

presence of CK may indicate heart attack, what is the enzyme CK?

(a)  

15.

presence of this enzyme may indicate heart attack or hepatitis

(a)  

16.

presence of ALT may indicate hepatitis, what is the enzyme ALT?

(a)  

17.

presence of enzyme may indicate heart attack, liver damage

(a)  

18.

presence of enzyme may indicate acute pancreatitis

(a)  

19.

presence of enzyme may indicate monocytic leukemia

(a)  

20.

directly absorbs into the blood

a)

water-soluble vitamins

b)

fat-soluble vitamins

c)

both

d)

neither

21.

inorganic

a)

water-soluble vitamins

b)

fat-soluble vitamins

c)

both

d)

neither

22.

Essential in small amounts

a)

water-soluble vitamins

b)

fat-soluble vitamins

c)

both

d)

neither

23.

kidneys remove excess in urine

a)

water-soluble vitamins

b)

fat-soluble vitamins

c)

both

d)

neither

24.

needed in periodic doses

a)

water-soluble vitamins

b)

fat-soluble vitamins

c)

both

d)

neither

25.

function as coenzyme

a)

water-soluble vitamins

b)

fat-soluble vitamins

c)

both

d)

neither

26.

what disease causes weakness, feeling tired, changes to hair, sore arms and legs, gum disease, easy bleeding when you're deficient in Vitamin C

(a)  

27.

what disease will you get when you lack in thiamine or vitamin B1

(a)  

28.

what disease will you get when you lack in riboflavin or Vitamin B2

(a)  

29.

what disease will you get when you lack in niacin or B3

(a)  

30.

Vitamin B5 is also known as

(a)  

31.

Vitamin B6 that is found in plants

(a)  

32.

Vitamin B7 is also known as

(a)  

33.

Deficiency in (a)   or Vitamin B9 can lead to megaloblastic anemia

34.

Vitamin B12 is also known as

(a)  

35.

All are caused by lack in Vitamin A except...

a)

Night blindneserophthalmias

b)

xerophthalmia

c)

Bitot’s spots, skeratomalacia

d)

Carotenosis

36.

Deficiency in Vitamin D may lead to

(a)  

37.

Sodium related diseases

a)

Hypokalemia

b)

Hypercalcemia

c)

Both

d)

Neither

38.

Which of the following is one of the earliest enzyme to be discovered

a)

trypsin

b)

sucrase

c)

Both

d)

Neither

39.

Noncompetitive inhibitors are examples of this activity

a)

activation of zymogens

b)

allosteric regulation

c)

genetic control 

d)

all of the above

40.

A. all enzymes are globular proteins. 

B. Catalysts increase the rate of chemical reactions without being used up in the process.

a)

AB = T

b)

AB = F

c)

A = T ; B = F

d)

A = F ; B = T

41.

Which of the following is false?

a)

Optimum pH for enzymes is 7

b)

Optimum temperature for enzymes is 37 degrees Celsius

c)

More substrate means more enzyme activity which is a straight line

d)

More enzyme means more enzyme activity which is a straight line

42.

When the temperature and pH level increases beyond the optimum level to the extremes, the enzyme becomes?

(a)  

43.

1 enzyme is to 1 group of substrate

a)

Absolute Specificity

b)

Relative Specificity

c)

Stereochemical Specificity

d)

Stereophysical

Specificity

44.

apoenzyme is the term used for the protein portion of an enzyme

a)

cofactor

b)

coenzyme

c)

prosthetic group

d)

true

45.

The chemical transformation of the substrate occurs at the active site, aided by functional groups on the enzyme that participate in the making and breaking of chemical bonds. After this, what is released?

a)

product

b)

substrate

c)

coenzyme

d)

apoenzyme

46.

High specificity is to Lock and Key Theory just like flexible conformation is to?

(a)  

47.

occurs when an inhibitor forms a covalent bond with a specific functional group of an enzyme, thereby inactivating it. 

(a)  

48.

Frank is a reversible enzyme competitor, he binds in the active sight to prevent the happening of enzyme substrate complex. what kind of reversible competitor is he?

(a)  

49.

A. Isoenzymes are slightly different forms of the same enzyme produced by different tissues

B. Noncompetitive inhibitor bind reversibly to the enzyme at a site other than the active site, changing the 3D shape of the enzyme and the active site, so that the normal substrate no longer fits correctly

a)

AB = T

b)

AB = F

c)

A = T ; B = F

d)

A = F ; B = T

50.

A. RNA is found mainly in the cytoplasm of living cells

B. DNA and RNA are polymers consisting of 2 repeating subunits called nucleotides , which are made of three components

a)

AB = T

b)

AB = F

c)

A = T ; B = F

d)

A = F ; B = T

51.

A. Uracil is a pyrimidine have two carbon nitrogen rings.

B. The bases covalent bond to each other in a specific way: A covalent bonds to T, and G covalent bonds to C, forming a set of complementary base pairs

a)

AB = T

b)

AB = F

c)

A = T ; B = F

d)

A = F ; B = T

52.

A. The base sequence of a DNA strand is always written from the 5′ end to the 3′ end.

B. normal human cell contains 36 chromosomes

a)

AB = T

b)

AB = F

c)

A = T ; B = F

d)

A = F ; B = T

53.

A. The Okazaki fragments along the lagging strand are joined by an enzyme called RNA Primer, which removes the RNA primer and replaces it with the correct nucleotides.

B. The gaps or breaks between segments in daughter strand at replication are called nicks, and the DNA fragments separated by the nicks are called Okazaki fragments

a)

AB = T

b)

AB = F

c)

A = T ; B = F

d)

A = F ; B = T

54.

This enzyme catalyzes the separation and unwinding of the nucleic acid strands at a specific point called a replication fork.

(a)  

55.

obtained an X-ray crystal structure (“Photo 51”) of a sample of DNA which contained structural features

a)

Friedrich Miescher

b)

Albrecht Kossel

c)

Rosalind Franklin

d)

James D. Watson and Francis H. C. Crick

e)

Oswald Avery, Colin MacLeod, and Maclyn McCarty

56.

an enzyme that synthesize DNA by the process of replication, which is important for maintaining and transferring genetic information from one generation to another

(a)  

57.

A. The central dogma of molecular biology states that DNA contains instructions for making a protein, which are copied by RNA. Then, RNA uses the instructions to make a protein.

B. PCR or Polymerase Chain Reaction can be used on degraded DNA samples

a)

AB = T

b)

AB = F

c)

A = T ; B = F

d)

A = F ; B = T

58.

delivers individual amino acids to the site of protein synthesis.

a)

dRNA

b)

tRNA

c)

mRNA

d)

mRNA

59.

A. tRNA is the smallest of nucleic acids and has a short life span

B. an enzyme called RNA polymerase proceeds along the DNA template adding nucleotides by base pairing with the DNA template in a manner similar to DNA replication, with the difference that an RNA strand is being synthesized that does not remain bound to the DNA template. 

a)

AB = T

b)

AB = F

c)

A = T ; B = F

d)

A = F ; B = T

60.

What is DNA to Proteins?

a)

A. Translation

b)

B. Transcription

c)

C. Replication

d)

D. Both A and B

61.

Start Codon which also codes for methionine

a)

AUG

b)

UAA

c)

UGA

d)

UAG

62.

Arrange the following from start to finish of Transcription

A. Initiation

B. Synthesis of DNA segments

C.  Unwinding of double helix

D. Chain elongation

E. Closing the nicks

F. Termination

a)

ADF

b)

ADE

c)

CBE

d)

CBF

63.

A. Degeneracy is a feature where most amino acids are represented by only one codon

B. Any changes resulting in an incorrect base sequence on DNA is considered as mutation

a)

AB = T

b)

AB = F

c)

A = T ; B = F

d)

A = F ; B = T

64.

A. Recombinant DNA is produced when segments of DNA from one organism are introduced into the genetic material of another organism.

B. Chemiosmosis is the movement of ions across a semipermeable membrane bound structure, down their electrochemical gradient

a)

AB = T

b)

AB = F

c)

A = T ; B = F

d)

A = F ; B = T

65.

Citrate is an intermediate of the citric acid cycle

a)

Oxaloacetate

b)

Isocitrate

c)

True

d)

Acetyl CoA