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WorksheetsHaematology- CBC, DLC, Haemocytometer, Hb estimation
Total questions: 133
Worksheet time: 1hrs 7mins
What is the another name of complete blood count in hematology?
full blood count (FBC)
complete blood cell count
full blood exam (FBE)
all of the above
Which is the most commonly performed test in medicine that provide an overview of patient's general health status?
sputum test
stool test
blood test
urine test
For complete blood count (CBC) which specimen is used?
blood plasma
uncentrifuged whole blood with EDTA
stool sample
none of the above
What is a differential leukocyte count (DLC) test done for?
to calculate the percentage of each WBC in blood
to calculate the percentage of RBC's in blood
to calculate the percentage of plasma in blood
to calculate the percentage of platelets in blood
What are the indications for CBC in hematology?
differentiation of anemias
serve as quality control checks
explain health issues and symptoms like weakness, fever , bruising
see the effect of medications
all of the above
What is the unit of volume of whole blood taken for CBC?
per cu mm
per microlitre
per mm Hg
both A & B
both B & C
In CBC, What is the normal value/ range is of Red blood cell count for men?
1 to 1.5 million cells/mcl
2.5 - 4.5 million cells/ mcl
4.5 - 6.5 millions cells/ mcl
7- 10 million cells/ mcl
What is the normal range of red blood cell count in women?
5 million to 10 million cells/mcl
1.5 to 2.5 million cells/mcl
3.8 - 5.8 million/mcl
none of the above
What is the normal range of platelet in blood (CBC)?
10,000 to 50,000 platelets/mcl
150,000 to 450,000 platelets/mcl
100,000 to 400,000 platelets /mcl
500,000 to 650,000 platelets/mcl
What is the normal range of WBC's in blood (CBC)?
2000 to 8000 per microlitre of blood
4000 to 11,000 per microlitre of blood
11000 to 15,000 per microlitre of blood
none of the above
What is the normal range of Haemoglobin in women?
11 to 13 gm/dl
12 to 16.5 gm/dl
16 to 18 gm/dl
10 to 15 gm/dl
What is the normal range of Haemoglobin for men?
10 - 13 gm/dl
10-14 gm/dl
12-14 gm/dl
14-18 gm/dl
What is the unit of hemoglobin?
per microlitre
grams per mm Hg
grams per decilitre
all of the above
Hemoglobin is a protein in our blood that holds the oxygen?
true
false
What is the hematocrit / PCV (packed cell volume) of blood?
percentage of hemoglobin in blood
percent of WBC in blood
Both A & B
proportion of blood that is made up of cells
all of the above
What is the normal range of PCV (packed cell volume) 9n men?
41.5% to 52.4%
10% to 15%
15% to 30%
35% to 60 %
What is the normal range of PCV (packed cell volume ) in women?
17% to 27%
37% to 47%
47% to 57%
17% to 27%
A high score in PCV (packed cell volume) in complete blood count indicates?
Too little iron to produce red blood cells
dehydrated or have other condition
What is the normal range of mean cell volume(MCV) in blood?
110-150 fl (femoliters)
80-100 fL
50-60fL
20-50 fL
If the MCV is between 80-100 fL then the red cells are called?
microcytic
normocytic
macrocytic
If MCV <80fL (abnormally small) , the RBC are called as?
normocytic
macrocytic
microcytic
If MCV >100fL (abnormally large) RBC are called as?
microcytic
macrocytic
normocytic
What is the range of MCV in iron deficiency anemia or thalessemia?
between 80-100fL
MCV >100fL
MCV <80fL
What is the range of MCV in vitamin B12 and folate deficiency anemia?
between 80-100fL
MCV >100fL
MCV >80fL
Mean cell hemoglobin conv=centration (MCHC) is the measure of the average concentration of hemoglobin inside a single red blood cell?
false
true
What is the normal range of MCHC in blood?
42- 66 g/dl
02-16g/dl
12-26g/dl
32-36g/dl
If the MCHC is normal , the cell is?
hyperchromic
hypochromic
normochromic
MCHC is used to differentiate normochromic from ----- anemia?
hyperchromic
hypochromic
In iron deficiency anemia and in thalessemia , the MCHC are less than normal , so the cells are?
hypochromic
hyperchromic
There are hyperchromic RBCs in blood?
true
false
Mean cell hemoglobin (MCH) indicate?
protein in your blood
the number of WBC
proportion of blood that is made up of cells
mean of Hb content in RBC
What is the normal range of MCH in blood?
27-32 pg
17-22 pg
07-12 pg
47-52 pg
If MCH is high, shows ----- RBCs?
Microcytic
Macrocytic
Microcytic red cells would have a ---- MCH value?
low MCH value
High MCH value
Red cell distribution width (RDW) reflects the degree of variation in size and shape of red blood cells?
true
false
Red cell distribution width (RDW) is calculated by?
automated analyzers
manual analyzers
Anisocytosis indicates what the level of RDW (red cell distrubution width)?
low RDW
High RDW
What is the normal range of ESR in CBC for men?
0-44 mm/hr
0-42mm/hr
0-22 mm/hr
0-55 mm/hr
What is the normal range of ESR in CBC for women?
0-19 mm/hr
0-29mm/hr
0-39mm/hr
0-59mm/hr
Normally , red blood cells settle relatively slowly, A faster than normal rate in ESR may indicates?
inflammation in the body
low hemoglobin
high count of WBC
all of the above
Which test measures how quickly erythrocytes (RBC) settle at the bottom of a test tube that contains a blood sample?
red cell count
MCHC
PCV
ESR
Which type of nucleated cells are present in WBC's that can be found in circulation?
heterogenous
homogenous
What is the role of WBC in human body?
Phagocytosis and immunity
Defense against infection
Fight from diseases/ foreign bodies
All of the above
WBCs are found in?
kidney
blood
liver
stomach
Which type of abnormal morphology is seen in neutropils in severe bacterial infection?
coarse granules in cytoplasm
small & regular granules in cytoplasm
no granules
none of the above
Presence of vacuoles in neutrophils indicates which type of condition?
bacterial infection
leukemia
phagocytosis
all of the above
In which condition / disease, premature leukocytes called blast cells are present?
leukaemia
neutropenia
eosinophil
Basophil
Which is the most common white blood cells (WBC) in peripheral blood smear?
basophil
neutrophil
eosinophil
monocyte
What is the diameter of Neutrophils?
6-12 micromitre
3-6 micromitre
12-15 micromitre
4-8 micromitre
Which type of WBC has a single nucleus with several indentations resulting in the nucleus being divided into multiple lobes , which are also called polymorphs?
eosinophil
basophil
Monocyte
Neutrophil
In neutrophils , the granules are very fine and stain lightly with both acidic (pink red) and basic (blue) dyes?
true
false
What is the normal percentage of neutrophil in blood smear?
5-10%
60-70%
85-90%
2-3%
What is the absolute count per microlitre of neutrophils?
50-100
1000-1200
400-800
3000-6000
Which type of white blood cell are also called polymorphs?
Eosinophils
Basophils
Neutrophils
Lymphocytes
What is the normal percentage of eosinophil present in blood smear?
45-50%
60-70%
4-5%
1-3%
What is the absolute count per microlitre of Eosiniphil in blood smear?
1000-2000
100-400
3000-4000
200-800
Which type of white blood cell have large granules and stai a bright red with acidic dyes (like eosin)?
Basophil
Lymphocyte
Neutrophil
Eosinophil
Which type of white blood cells are rarely seen in peripheral blood?
Lympocyte
Eosinophil
basophil
Monocytes
Which white blood cell have the cytoplasm contains large coarse granules that stain blue purple with basic dyes and are PAS positive?
Neutrophils
Eosinophils
RBCs
Basophils
What is the normal percentage os Basophils present in blood smear?
2-3%
0-1%
15-20%
60-70%
Which is the most common type of white blood cell in the peripheral blood?
Eosinophil
Basophil
Lymphocyte
Neutrophils
What is the normal percentage of Lymphocytes in blood smear?
20-30%
70-80%
15-20%
1-2%
Which type of WBC have scanty of cytoplasm and forms a thin peripheral rim around the nucleus . It is clear blue in colour (basophilic)and granules are absent?
Neutrophils
Basophils
Lymphocytes
Monocytes
Which type of WBC has absolute count per microlitre 1500-3000?
Lymphocyte
Basophils
Eosinophils
Neutrophils
Which type of WBC normally absent or present in low numbers in the peripheral blood?
monocytes
lymphocytes
Neutrophil
All of the above
In which type of WBC , the nucleus iss present ecentrically?
Basophil
Monocytes
Lymphocytes
Neutrophil
Which WBCs the cytoplasm are like frosted glass in appearance?
Monocytes
Lymphocytes
Basophils
Eosinophil
What is the mormal percentage of monocytes in peripheral blood smear?
30-40%
1-5%
10-15%
8-10%
What is the absolute count of monocytes?
900-1200
1000-1200
100-700
3000-4000
In DLC , each cells are observed in microscope and the counting is continued till ------ cells to give the average number of white blood cells.
1000
100
10
200
In DLC recording, which methods can be used?
record on cell counter
record on a piece of paper in tabular form
record in memory
all of the above
both A & B
With Leishman stain, the cells are observed as?
platelets: violet coloured granules
Neutrophil: purple coloured nuclei with pink cytoplasm
red cell: pink colour
all of the above
Violet coloured granules are present in which cell, if stained with Leishman stain?
RBC
Platelets
Lymphocytes
Neutrophil
What is the main clinical significance of DLC?
to diagnose malignant blood disorders
diagnosis of infections
diagnosis of inflammatory conditions
diagnosis of allergic reactions
all of the above
What is the normal count of neutrophil in percentage?
20-30%
60-70%
6-7%
80-90%
What is the normal value of lymphocytes in percentage?
60-70%
20-30%
6-7%
0-2%
What is the normal count of neutrophil in per cu mm?
100-400
3000-6000
1500-3000
5000-8000
What is the normal value of basophil in percentage?
20-30%
6-7%
60-70%
0-1%
What is the meaninig of the term penia used as suffix in medical terms?
increased in number
lack or to have a deficiency
affection
more than normal
What is the meaning of the term Lymphocytosis?
Decrease in Lymphocytes
Increase in Lymphocytes
What is the meaning of the term Neutrophilia?
Increased number of Neutrophils
Decreased number of Neutrophils
The term philia is used in medical terms to indicate?
Increased condition
More than normal
Decreased condition
Lack or deficiency
Both A & B
The term monocytosis is used in which condition?
Increase in monocyte count
Decrease in monocyte count
The term Basopenia means?
More than normal count of basophil
Less than normal count of basophil
Which technique is used to count the total cell in blood or some other biological fluids?
Hemoglobin
Haemocytometry
Photocytometry
Nanocytometry
Which equipment is used to count the total cell present in blood manually?
Haemometer
Globulocytometer
Haematocytometer
Microscope
What are the advantages of haemocytometer?
Simplest method
most convenient
cheap
all of the above
In the term haemocytometer , the word haemo means?
fluid
blood
cell
all of the above
In the term Haemocytometer , the word cyto means?
cell
blood
fluid
tissue
Which of the following types of equipment are present in a haemocytometer box?
Neubauer's counting chamber
coverslips
2 pipettes
diluting fluids
all of the above
What are the 2 types of pipettes used for diluting samples in haemocytometer?
cell & tissue pipette
white pipette and red pipette
RBC pipette & WBC pipette
Both B & C
The coverslips in the haemocytometer box are placed over the ------ ?
RBC Pipette
WBC pipette
Neubauer's Counting chamber
WBC & RBC pipettes have different colored mouthpiece?
true
false
Neubauer's slide with a coverslip over it is called?
coverslip
haemo chamber
slides chamber
Neubauer's chamber
How many vertical troughs are there in the counting chamber (Neubauer's chamber)?
2
4
6
5
How many horizontal trough are present in Neubauer's chamber of Haematocytometer?
1
2
4
3
Which area in Neubauer's chamber have counting grid area used for counting cells in haemocytometer?
side walls
central platform
What is the width & length of each large square in counting grid areas of Neubaur's chambers?
3mm x 3mm
2mm x 3mm
2mm x 2 mm
4mm x 4mm
What are the dimensions of each of the smaller squares in the counting grid area of Neubauer's chamber?
2 mm long & 2 mm wide
2mm long & 1 mm wide
2 mm long & 4mm wide
1 mm long & 1 mm wide
In counting grid of Neubauer's chamber in haematocytometer , the larger square is divided into how many smaller squares?
6
9
8
10
What is the total number if smallest squares present at four corners in the counting chamber of Haematocytometer?
40
12
64
32
What is the width of one corner square in counting chamber used for counting WBC?
1mm
0.5mm
3mm
5mm
What is the depth of Neubauer's chamber in hemocytometer?
0.5mm
0.1mm
1mm
4mm
How will you calculate the volume of diluted blood in 4 small squares?
Area x height
Area x depth
No. of cell x area
none of the above
In counting chamber (Neubauer's chamber) in haemocytometer , the central square is sub divided into how many even smaller squares?
6
2
4
5
In haemocytometer , which area in counting chamber is used for counting platelets?
4 corner square
central square
one corner square in central square
all of the above
What is the length of one corner small square of central square used for calculating RBC count?
1/8 mm
1/5mm
1/10mm
1/4mm
What is the depth of the chamber in counting grid?
0.6mm
0.4mm
0.1mm
0.2mm
What is the volume of diluted blood in 4 corner and 1 central small square will be = Area x depth , so what is the volume of diluted blood?
1/20mm
1/40mm
1/60mm
1/50mm
What are the errors that can occur during Haemocytometery / cell counting?
Improper filling of chambers
Human error
statistical error
All of the above
In case of error due to non uniform suspension used in cell counting. How it is prevented /corrected?
Fill the chamber properly
Repeat the process unless 90% or more cells are free from contact with other cells
cover the chamber with air tight slip cover
all of the above
In haempcytometer , the chamber is filled properly by ----- action?
capillary
venous
arterial
blood pressure
What will you decide, to count and what not to count concerning a cell on a border of chamber in Haemocytometer?
count all the cells at border
do not count all the cells at border
do not count half of the cell that touches a border
all of the above
Careful attention can reduce statistical error between ----- &-----?
25% & 50%
1% & 5%
15% & 20%
10% & 15%
The person counting cells needs to be careful and attentive . All required squares should be counted properly. This can reduce which type of error?
Statistical error
human error
Improper filling of chambers
Non uniform suspensions
What would happen to red blood cells if the haem group was removed from hemoglobin?
Red blood cells would not be able to bind oxygen
Red blood cells would not be able to reproduce
white blood cells would not be able to reproduce
blood clot formation would be inhibited
Hemoglobin transports oxygen from ----- to all parts of the body?
Heart
lungs
kidney
mouth
Hemoglobin transports ---- from all parts of the body back to the lungs?
oxygen
carbon dioxide
nitrogen
waste materials
Hb estimation in the blood can be done by?
Estimating oxygen binding capacity
Estimating color change
Estimating iron content
All of the above
Colorimetric method of hemoglobin estimation does not include?
Sahli's acid hematin method
photoelectric method
Haematocytometer method
cyanmethemoglobin method
Hoe much blood sample is taken from the micropipette to detect hemoglobin in Sahli's method?
10 microlitre
20 microlitre
10gm
20gm
In Sahli's method , acid hematin formed is of?
black color
red color
dark brown color
greyish color
What is the unit of hemoglobin?
gm/dl
mmHg
Microlitre
mm/dl
In Sahli's hemoglobinometer method, matching of color should be done against all except?
natural light
white light
dark background
electrical tube light
Sahli's method is less accurate because of ----- errors?
subjective
calculation
visual
human
In sahli's method, blood sample and ----- HCL acid should be taken in an accurate and precise amount in the Hb tube?
N/100
N/20
N/10
N/15
Cyanmethemoglobin method of hemoglobin estimation is based on?
The color of the formed acid hematin complex that corresponds to the hemoglobin concentration in the blood
formation of methemoglobin by addition of potassium cyanide
Oxygen binding capacity of blood
iron content of blood
Which of the following reagent is used in Cyanmethemoglobin method of hemoglobin detection?
Hydrochloric acid
Hydrogen peroxide
Drabkin's solution
any of these
What is true about Drabkin's solution except?
used for hemoglobin estimation
Clear, pale yellow liquid, unstable to light and stored in refrigerator
Drabkin's solution is pipetted by mouth for Hb estimation
Absorbance of solution must be zero at a wavelength of 540nm
What all make Drabkin's reagent?
potassium ferricyanide
potassium cyanide
potassium dihydrogen phosphate
All of the above
THe following are the benefits of cyanmethemoglobin method over Sahli's method of hemoglobin estimation except?
Internationally accepted
All forms of Hb except sulphemoglobin are converted to cyanmethemoglobin
If the color matching point is passed, the whole procedure has to be repeated
more accurate and reliable
What concentration of Drabkin's solution is used in the cyanmethemoglobin method of hemoglobin detection?
4 ml
5 ml
5.5 ml
6ml
In cyanmethemoglobin method of hemoglobin estimation, absorbance of the solution is measured as?
440nm
540nm
640nm
450nm
