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Haematology- CBC, DLC, Haemocytometer, Hb estimation

Total questions: 133

Worksheet time: 1hrs 7mins

Name
Class
Date
1.

What is the another name of complete blood count in hematology?

a)

full blood count (FBC)

b)

complete blood cell count

c)

full blood exam (FBE)

d)

all of the above

2.

Which is the most commonly performed test in medicine that provide an overview of patient's general health status?

a)

sputum test

b)

stool test

c)

blood test

d)

urine test

3.

For complete blood count (CBC) which specimen is used?

a)

blood plasma

b)

uncentrifuged whole blood with EDTA

c)

stool sample

d)

none of the above

4.

What is a differential leukocyte count (DLC) test done for?

a)

to calculate the percentage of each WBC in blood

b)

to calculate the percentage of RBC's in blood

c)

to calculate the percentage of plasma in blood

d)

to calculate the percentage of platelets in blood

5.

What are the indications for CBC in hematology?

a)

differentiation of anemias

b)

serve as quality control checks

c)

explain health issues and symptoms like weakness, fever , bruising

d)

see the effect of medications

e)

all of the above

6.

What is the unit of volume of whole blood taken for CBC?

a)

per cu mm

b)

per microlitre

c)

per mm Hg

d)

both A & B

e)

both B & C

7.

In CBC, What is the normal value/ range is of Red blood cell count for men?

a)

1 to 1.5 million cells/mcl

b)

2.5 - 4.5 million cells/ mcl

c)

4.5 - 6.5 millions cells/ mcl

d)

7- 10 million cells/ mcl

8.

What is the normal range of red blood cell count in women?

a)

5 million to 10 million cells/mcl

b)

1.5 to 2.5 million cells/mcl

c)

3.8 - 5.8 million/mcl

d)

none of the above

9.

What is the normal range of platelet in blood (CBC)?

a)

10,000 to 50,000 platelets/mcl

b)

150,000 to 450,000 platelets/mcl

c)

100,000 to 400,000 platelets /mcl

d)

500,000 to 650,000 platelets/mcl

10.

What is the normal range of WBC's in blood (CBC)?

a)

2000 to 8000 per microlitre of blood

b)

4000 to 11,000 per microlitre of blood

c)

11000 to 15,000 per microlitre of blood

d)

none of the above

11.

What is the normal range of Haemoglobin in women?

a)

11 to 13 gm/dl

b)

12 to 16.5 gm/dl

c)

16 to 18 gm/dl

d)

10 to 15 gm/dl

12.

What is the normal range of Haemoglobin for men?

a)

10 - 13 gm/dl

b)

10-14 gm/dl

c)

12-14 gm/dl

d)

14-18 gm/dl

13.

What is the unit of hemoglobin?

a)

per microlitre

b)

grams per mm Hg

c)

grams per decilitre

d)

all of the above

14.

Hemoglobin is a protein in our blood that holds the oxygen?

a)

true

b)

false

15.

What is the hematocrit / PCV (packed cell volume) of blood?

a)

percentage of hemoglobin in blood

b)

percent of WBC in blood

c)

Both A & B

d)

proportion of blood that is made up of cells

e)

all of the above

16.

What is the normal range of PCV (packed cell volume) 9n men?

a)

41.5% to 52.4%

b)

10% to 15%

c)

15% to 30%

d)

35% to 60 %

17.

What is the normal range of PCV (packed cell volume ) in women?

a)

17% to 27%

b)

37% to 47%

c)

47% to 57%

d)

17% to 27%

18.

A high score in PCV (packed cell volume) in complete blood count indicates?

a)

Too little iron to produce red blood cells

b)

dehydrated or have other condition

19.

What is the normal range of mean cell volume(MCV) in blood?

a)

110-150 fl (femoliters)

b)

80-100 fL

c)

50-60fL

d)

20-50 fL

20.

If the MCV is between 80-100 fL then the red cells are called?

a)

microcytic

b)

normocytic

c)

macrocytic

21.

If MCV <80fL (abnormally small) , the RBC are called as?

a)

normocytic

b)

macrocytic

c)

microcytic

22.

If MCV >100fL (abnormally large) RBC are called as?

a)

microcytic

b)

macrocytic

c)

normocytic

23.

What is the range of MCV in iron deficiency anemia or thalessemia?

a)

between 80-100fL

b)

MCV >100fL

c)

MCV <80fL

24.

What is the range of MCV in vitamin B12 and folate deficiency anemia?

a)

between 80-100fL

b)

MCV >100fL

c)

MCV >80fL

25.

Mean cell hemoglobin conv=centration (MCHC) is the measure of the average concentration of hemoglobin inside a single red blood cell?

a)

false

b)

true

26.

What is the normal range of MCHC in blood?

a)

42- 66 g/dl

b)

02-16g/dl

c)

12-26g/dl

d)

32-36g/dl

27.

If the MCHC is normal , the cell is?

a)

hyperchromic

b)

hypochromic

c)

normochromic

28.

MCHC is used to differentiate normochromic from ----- anemia?

a)

hyperchromic

b)

hypochromic

29.

In iron deficiency anemia and in thalessemia , the MCHC are less than normal , so the cells are?

a)

hypochromic

b)

hyperchromic

30.

There are hyperchromic RBCs in blood?

a)

true

b)

false

31.

Mean cell hemoglobin (MCH) indicate?

a)

protein in your blood

b)

the number of WBC

c)

proportion of blood that is made up of cells

d)

mean of Hb content in RBC

32.

What is the normal range of MCH in blood?

a)

27-32 pg

b)

17-22 pg

c)

07-12 pg

d)

47-52 pg

33.

If MCH is high, shows ----- RBCs?

a)

Microcytic

b)

Macrocytic

34.

Microcytic red cells would have a ---- MCH value?

a)

low MCH value

b)

High MCH value

35.

Red cell distribution width (RDW) reflects the degree of variation in size and shape of red blood cells?

a)

true

b)

false

36.

Red cell distribution width (RDW) is calculated by?

a)

automated analyzers

b)

manual analyzers

37.

Anisocytosis indicates what the level of RDW (red cell distrubution width)?

a)

low RDW

b)

High RDW

38.

What is the normal range of ESR in CBC for men?

a)

0-44 mm/hr

b)

0-42mm/hr

c)

0-22 mm/hr

d)

0-55 mm/hr

39.

What is the normal range of ESR in CBC for women?

a)

0-19 mm/hr

b)

0-29mm/hr

c)

0-39mm/hr

d)

0-59mm/hr

40.

Normally , red blood cells settle relatively slowly, A faster than normal rate in ESR may indicates?

a)

inflammation in the body

b)

low hemoglobin

c)

high count of WBC

d)

all of the above

41.

Which test measures how quickly erythrocytes (RBC) settle at the bottom of a test tube that contains a blood sample?

a)

red cell count

b)

MCHC

c)

PCV

d)

ESR

42.

Which type of nucleated cells are present in WBC's that can be found in circulation?

a)

heterogenous

b)

homogenous

43.

What is the role of WBC in human body?

a)

Phagocytosis and immunity

b)

Defense against infection

c)

Fight from diseases/ foreign bodies

d)

All of the above

44.

WBCs are found in?

a)

kidney

b)

blood

c)

liver

d)

stomach

45.

Which type of abnormal morphology is seen in neutropils in severe bacterial infection?

a)

coarse granules in cytoplasm

b)

small & regular granules in cytoplasm

c)

no granules

d)

none of the above

46.

Presence of vacuoles in neutrophils indicates which type of condition?

a)

bacterial infection

b)

leukemia

c)

phagocytosis

d)

all of the above

47.

In which condition / disease, premature leukocytes called blast cells are present?

a)

leukaemia

b)

neutropenia

c)

eosinophil

d)

Basophil

48.

Which is the most common white blood cells (WBC) in peripheral blood smear?

a)

basophil

b)

neutrophil

c)

eosinophil

d)

monocyte

49.

What is the diameter of Neutrophils?

a)

6-12 micromitre

b)

3-6 micromitre

c)

12-15 micromitre

d)

4-8 micromitre

50.

Which type of WBC has a single nucleus with several indentations resulting in the nucleus being divided into multiple lobes , which are also called polymorphs?

a)

eosinophil

b)

basophil

c)

Monocyte

d)

Neutrophil

51.

In neutrophils , the granules are very fine and stain lightly with both acidic (pink red) and basic (blue) dyes?

a)

true

b)

false

52.

What is the normal percentage of neutrophil in blood smear?

a)

5-10%

b)

60-70%

c)

85-90%

d)

2-3%

53.

What is the absolute count per microlitre of neutrophils?

a)

50-100

b)

1000-1200

c)

400-800

d)

3000-6000

54.

Which type of white blood cell are also called polymorphs?

a)

Eosinophils

b)

Basophils

c)

Neutrophils

d)

Lymphocytes

55.

What is the normal percentage of eosinophil present in blood smear?

a)

45-50%

b)

60-70%

c)

4-5%

d)

1-3%

56.

What is the absolute count per microlitre of Eosiniphil in blood smear?

a)

1000-2000

b)

100-400

c)

3000-4000

d)

200-800

57.

Which type of white blood cell have large granules and stai a bright red with acidic dyes (like eosin)?

a)

Basophil

b)

Lymphocyte

c)

Neutrophil

d)

Eosinophil

58.

Which type of white blood cells are rarely seen in peripheral blood?

a)

Lympocyte

b)

Eosinophil

c)

basophil

d)

Monocytes

59.

Which white blood cell have the cytoplasm contains large coarse granules that stain blue purple with basic dyes and are PAS positive?

a)

Neutrophils

b)

Eosinophils

c)

RBCs

d)

Basophils

60.

What is the normal percentage os Basophils present in blood smear?

a)

2-3%

b)

0-1%

c)

15-20%

d)

60-70%

61.

Which is the most common type of white blood cell in the peripheral blood?

a)

Eosinophil

b)

Basophil

c)

Lymphocyte

d)

Neutrophils

62.

What is the normal percentage of Lymphocytes in blood smear?

a)

20-30%

b)

70-80%

c)

15-20%

d)

1-2%

63.

Which type of WBC have scanty of cytoplasm and forms a thin peripheral rim around the nucleus . It is clear blue in colour (basophilic)and granules are absent?

a)

Neutrophils

b)

Basophils

c)

Lymphocytes

d)

Monocytes

64.

Which type of WBC has absolute count per microlitre 1500-3000?

a)

Lymphocyte

b)

Basophils

c)

Eosinophils

d)

Neutrophils

65.

Which type of WBC normally absent or present in low numbers in the peripheral blood?

a)

monocytes

b)

lymphocytes

c)

Neutrophil

d)

All of the above

66.

In which type of WBC , the nucleus iss present ecentrically?

a)

Basophil

b)

Monocytes

c)

Lymphocytes

d)

Neutrophil

67.

Which WBCs the cytoplasm are like frosted glass in appearance?

a)

Monocytes

b)

Lymphocytes

c)

Basophils

d)

Eosinophil

68.

What is the mormal percentage of monocytes in peripheral blood smear?

a)

30-40%

b)

1-5%

c)

10-15%

d)

8-10%

69.

What is the absolute count of monocytes?

a)

900-1200

b)

1000-1200

c)

100-700

d)

3000-4000

70.

In DLC , each cells are observed in microscope and the counting is continued till ------ cells to give the average number of white blood cells.

a)

1000

b)

100

c)

10

d)

200

71.

In DLC recording, which methods can be used?

a)

record on cell counter

b)

record on a piece of paper in tabular form

c)

record in memory

d)

all of the above

e)

both A & B

72.

With Leishman stain, the cells are observed as?

a)

platelets: violet coloured granules

b)

Neutrophil: purple coloured nuclei with pink cytoplasm

c)

red cell: pink colour

d)

all of the above

73.

Violet coloured granules are present in which cell, if stained with Leishman stain?

a)

RBC

b)

Platelets

c)

Lymphocytes

d)

Neutrophil

74.

What is the main clinical significance of DLC?

a)

to diagnose malignant blood disorders

b)

diagnosis of infections

c)

diagnosis of inflammatory conditions

d)

diagnosis of allergic reactions

e)

all of the above

75.

What is the normal count of neutrophil in percentage?

a)

20-30%

b)

60-70%

c)

6-7%

d)

80-90%

76.

What is the normal value of lymphocytes in percentage?

a)

60-70%

b)

20-30%

c)

6-7%

d)

0-2%

77.

What is the normal count of neutrophil in per cu mm?

a)

100-400

b)

3000-6000

c)

1500-3000

d)

5000-8000

78.

What is the normal value of basophil in percentage?

a)

20-30%

b)

6-7%

c)

60-70%

d)

0-1%

79.

What is the meaninig of the term penia used as suffix in medical terms?

a)

increased in number

b)

lack or to have a deficiency

c)

affection

d)

more than normal

80.

What is the meaning of the term Lymphocytosis?

a)

Decrease in Lymphocytes

b)

Increase in Lymphocytes

81.

What is the meaning of the term Neutrophilia?

a)

Increased number of Neutrophils

b)

Decreased number of Neutrophils

82.

The term philia is used in medical terms to indicate?

a)

Increased condition

b)

More than normal

c)

Decreased condition

d)

Lack or deficiency

e)

Both A & B

83.

The term monocytosis is used in which condition?

a)

Increase in monocyte count

b)

Decrease in monocyte count

84.

The term Basopenia means?

a)

More than normal count of basophil

b)

Less than normal count of basophil

85.

Which technique is used to count the total cell in blood or some other biological fluids?

a)

Hemoglobin

b)

Haemocytometry

c)

Photocytometry

d)

Nanocytometry

86.

Which equipment is used to count the total cell present in blood manually?

a)

Haemometer

b)

Globulocytometer

c)

Haematocytometer

d)

Microscope

87.

What are the advantages of haemocytometer?

a)

Simplest method

b)

most convenient

c)

cheap

d)

all of the above

88.

In the term haemocytometer , the word haemo means?

a)

fluid

b)

blood

c)

cell

d)

all of the above

89.

In the term Haemocytometer , the word cyto means?

a)

cell

b)

blood

c)

fluid

d)

tissue

90.

Which of the following types of equipment are present in a haemocytometer box?

a)

Neubauer's counting chamber

b)

coverslips

c)

2 pipettes

d)

diluting fluids

e)

all of the above

91.

What are the 2 types of pipettes used for diluting samples in haemocytometer?

a)

cell & tissue pipette

b)

white pipette and red pipette

c)

RBC pipette & WBC pipette

d)

Both B & C

92.

The coverslips in the haemocytometer box are placed over the ------ ?

a)

RBC Pipette

b)

WBC pipette

c)

Neubauer's Counting chamber

93.

WBC & RBC pipettes have different colored mouthpiece?

a)

true

b)

false

94.

Neubauer's slide with a coverslip over it is called?

a)

coverslip

b)

haemo chamber

c)

slides chamber

d)

Neubauer's chamber

95.

How many vertical troughs are there in the counting chamber (Neubauer's chamber)?

a)

2

b)

4

c)

6

d)

5

96.

How many horizontal trough are present in Neubauer's chamber of Haematocytometer?

a)

1

b)

2

c)

4

d)

3

97.

Which area in Neubauer's chamber have counting grid area used for counting cells in haemocytometer?

a)

side walls

b)

central platform

98.

What is the width & length of each large square in counting grid areas of Neubaur's chambers?

a)

3mm x 3mm

b)

2mm x 3mm

c)

2mm x 2 mm

d)

4mm x 4mm

99.

What are the dimensions of each of the smaller squares in the counting grid area of Neubauer's chamber?

a)

2 mm long & 2 mm wide

b)

2mm long & 1 mm wide

c)

2 mm long & 4mm wide

d)

1 mm long & 1 mm wide

100.

In counting grid of Neubauer's chamber in haematocytometer , the larger square is divided into how many smaller squares?

a)

6

b)

9

c)

8

d)

10

101.

What is the total number if smallest squares present at four corners in the counting chamber of Haematocytometer?

a)

40

b)

12

c)

64

d)

32

102.

What is the width of one corner square in counting chamber used for counting WBC?

a)

1mm

b)

0.5mm

c)

3mm

d)

5mm

103.

What is the depth of Neubauer's chamber in hemocytometer?

a)

0.5mm

b)

0.1mm

c)

1mm

d)

4mm

104.

How will you calculate the volume of diluted blood in 4 small squares?

a)

Area x height

b)

Area x depth

c)

No. of cell x area

d)

none of the above

105.

In counting chamber (Neubauer's chamber) in haemocytometer , the central square is sub divided into how many even smaller squares?

a)

6

b)

2

c)

4

d)

5

106.

In haemocytometer , which area in counting chamber is used for counting platelets?

a)

4 corner square

b)

central square

c)

one corner square in central square

d)

all of the above

107.

What is the length of one corner small square of central square used for calculating RBC count?

a)

1/8 mm

b)

1/5mm

c)

1/10mm

d)

1/4mm

108.

What is the depth of the chamber in counting grid?

a)

0.6mm

b)

0.4mm

c)

0.1mm

d)

0.2mm

109.

What is the volume of diluted blood in 4 corner and 1 central small square will be = Area x depth , so what is the volume of diluted blood?

a)

1/20mm

b)

1/40mm

c)

1/60mm

d)

1/50mm

110.

What are the errors that can occur during Haemocytometery / cell counting?

a)

Improper filling of chambers

b)

Human error

c)

statistical error

d)

All of the above

111.

In case of error due to non uniform suspension used in cell counting. How it is prevented /corrected?

a)

Fill the chamber properly

b)

Repeat the process unless 90% or more cells are free from contact with other cells

c)

cover the chamber with air tight slip cover

d)

all of the above

112.

In haempcytometer , the chamber is filled properly by ----- action?

a)

capillary

b)

venous

c)

arterial

d)

blood pressure

113.

What will you decide, to count and what not to count concerning a cell on a border of chamber in Haemocytometer?

a)

count all the cells at border

b)

do not count all the cells at border

c)

do not count half of the cell that touches a border

d)

all of the above

114.

Careful attention can reduce statistical error between ----- &-----?

a)

25% & 50%

b)

1% & 5%

c)

15% & 20%

d)

10% & 15%

115.

The person counting cells needs to be careful and attentive . All required squares should be counted properly. This can reduce which type of error?

a)

Statistical error

b)

human error

c)

Improper filling of chambers

d)

Non uniform suspensions

116.

What would happen to red blood cells if the haem group was removed from hemoglobin?

a)

Red blood cells would not be able to bind oxygen

b)

Red blood cells would not be able to reproduce

c)

white blood cells would not be able to reproduce

d)

blood clot formation would be inhibited

117.

Hemoglobin transports oxygen from ----- to all parts of the body?

a)

Heart

b)

lungs

c)

kidney

d)

mouth

118.

Hemoglobin transports ---- from all parts of the body back to the lungs?

a)

oxygen

b)

carbon dioxide

c)

nitrogen

d)

waste materials

119.

Hb estimation in the blood can be done by?

a)

Estimating oxygen binding capacity

b)

Estimating color change

c)

Estimating iron content

d)

All of the above

120.

Colorimetric method of hemoglobin estimation does not include?

a)

Sahli's acid hematin method

b)

photoelectric method

c)

Haematocytometer method

d)

cyanmethemoglobin method

121.

Hoe much blood sample is taken from the micropipette to detect hemoglobin in Sahli's method?

a)

10 microlitre

b)

20 microlitre

c)

10gm

d)

20gm

122.

In Sahli's method , acid hematin formed is of?

a)

black color

b)

red color

c)

dark brown color

d)

greyish color

123.

What is the unit of hemoglobin?

a)

gm/dl

b)

mmHg

c)

Microlitre

d)

mm/dl

124.

In Sahli's hemoglobinometer method, matching of color should be done against all except?

a)

natural light

b)

white light

c)

dark background

d)

electrical tube light

125.

Sahli's method is less accurate because of ----- errors?

a)

subjective

b)

calculation

c)

visual

d)

human

126.

In sahli's method, blood sample and ----- HCL acid should be taken in an accurate and precise amount in the Hb tube?

a)

N/100

b)

N/20

c)

N/10

d)

N/15

127.

Cyanmethemoglobin method of hemoglobin estimation is based on?

a)

The color of the formed acid hematin complex that corresponds to the hemoglobin concentration in the blood

b)

formation of methemoglobin by addition of potassium cyanide

c)

Oxygen binding capacity of blood

d)

iron content of blood

128.

Which of the following reagent is used in Cyanmethemoglobin method of hemoglobin detection?

a)

Hydrochloric acid

b)

Hydrogen peroxide

c)

Drabkin's solution

d)

any of these

129.

What is true about Drabkin's solution except?

a)

used for hemoglobin estimation

b)

Clear, pale yellow liquid, unstable to light and stored in refrigerator

c)

Drabkin's solution is pipetted by mouth for Hb estimation

d)

Absorbance of solution must be zero at a wavelength of 540nm

130.

What all make Drabkin's reagent?

a)

potassium ferricyanide

b)

potassium cyanide

c)

potassium dihydrogen phosphate

d)

All of the above

131.

THe following are the benefits of cyanmethemoglobin method over Sahli's method of hemoglobin estimation except?

a)

Internationally accepted

b)

All forms of Hb except sulphemoglobin are converted to cyanmethemoglobin

c)

If the color matching point is passed, the whole procedure has to be repeated

d)

more accurate and reliable

132.

What concentration of Drabkin's solution is used in the cyanmethemoglobin method of hemoglobin detection?

a)

4 ml

b)

5 ml

c)

5.5 ml

d)

6ml

133.

In cyanmethemoglobin method of hemoglobin estimation, absorbance of the solution is measured as?

a)

440nm

b)

540nm

c)

640nm

d)

450nm